DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

정안침요법(整顔鍼療法)을 응용하여 호전된 후천성 사경환자 치험 2례

박서영,김재수,홍의재,이윤경,이봉효,임성철,정태영,이경민,Park, Seo-Young,Kim, Jae-Su,Hong, Eui-Jae,Lee, Yoon-Kyoung,Lee, Bong-Hyo,Lim, Seong-Chul,Jung, Tae-Young,Lee, Kyung-Min 대한침구의학회 2008 대한침구의학회지 Vol.25 No.4

Objectives : This report intended to estimate effects of Jung-an acupuncture on the acquired torticollis caused by muscle contraction. Methods : The patients were treated by Jung-an acupuncture, herb medication and Physical treatment daily. The improvement of the patients was judged by Toronto Western Spasmodic Torticollis Rating Scale(TWSTRS), Tsui's score and Visual Analogue Scale(VAS). Results : After 7 days of treatment, the 2 patients' neck pain and cervical movement were improved. Also, TWSTRS score, Tsui's score and VAS were all reduced. Conclusions : As the Jung-an acupuncture's theological base is organized with meridian theory and myofacial release therapy, We considered the Jung-an acupuncture is effective not only for the skin disease but also for the muscle problem such as torticollis.

Environmental Microbiology / Microbial Diversity : Calcium Carbonate Precipitation by Bacillus and Sporosarcina Strains Isolated from Concrete and Analysis of the Bacterial Community of Concrete(s)

( Hyun Jung Kim ),( Hyo Jung Eom ),( Chulwoo Park ),( Jaejoon Jung ),( Bora Shin ),( Wook Kim ),( Namhyun Chung ),( In Geol Choi ),( Woojun Park ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.3

Microbially induced calcium carbonate precipitation (CCP) is a long-standing but re-emerging environmental engineering process for production of self-healing concrete, bioremediation, and long-term storage of CO2. CCP-capable bacteria, two Bacillus strains (JH3 and JH7) and one Sporosarcina strain (HYO08), were isolated from two samples of concrete and characterized phylogenetically. Calcium carbonate crystals precipitated by the three strains were morphologically distinct according to field emission scanning electron microscopy. Energy dispersive X-ray spectrometry mapping confirmed biomineralization via extracellular calcium carbonate production. The three strains differed in their physiological characteristics: growth at alkali pH and high NaCl concentrations, and urease activity. Sporosarcina sp. HYO08 and Bacillus sp. JH7 were more alkali- and halotolerant, respectively. Analysis of the community from the same concrete samples using barcoded pyrosequencing revealed that the relative abundance of Bacillus and Sporosarcina species was low, which indicated low culturability of other dominant bacteria. This study suggests that calcium carbonate crystals with different properties can be produced by various CCP-capable strains, and other novel isolates await discovery.

치매방지 작업기구 사용이 항산화효소 활성에 미치는 영향

조경혜,박정은,윤지윤,문세보,박효영,정인옥,이정원 서울여자대학교 자연과학연구소 2001 자연과학연구논문집 Vol.13 No.-

We developed an occupational therapy tool to maintain and improve the cognitive function for the elderly who have senile dementia. 86 elderly people took a mininal mental state examination (MMSE) for the therapy to perform the occupational therapy, and divided into three groups based on avarage ages and MMSE values. The occupational therapy was treated three times per week for 10 weeks. The results showed that there were significant differences in the performing scores before and after the treatment ; the scores for the occupational therapy were increased significantly (p<0.001) and the performing time was shortened from 6-week throughout 10-week regimen. This study was to examine the effect on the activity of antioxidant enzymes by occupational therapy. The activities of antioxidant enzymes such as catalase, Glutathion-6-phosphate dehydrogenase (G6PDH), and superoxide dismutase (SOD) were increased to the normal standard by the occupational therapy for the elderly having dementia. The recovery of activities of antioxidant enzymes was obvious in severe demented elderly compared to those in normal or mild demented elderly. These results suggest that the occupational therapy had an beneficial effect on the protection against the oxidative stress especially for severe demented elderly, and the occupational tool need to be modified so as to improve the protection against the oxidative stress for normal and/or mild demented elderly.

북한산국립공원 북한산성-인수봉 지역의 산림군집구조

추갑철 ( Gab Cheul Choo ),엄태원 ( Tae Won Um ),김갑태 ( Gab Tae Kim ),박삼봉 ( Sam Bong Park ),안효현 ( Hyo Hyeon An ),김남호 ( Nam Ho Kim ),김희정 ( Hee Jung Kim ) 한국환경생태학회 2008 한국환경생태학회지 Vol.22 No.2

북한산 국립공원지역의 식생구조를 파악하고자, 북한산성에서 인수봉에 이르는 지역에 16개의 방형구(2500㎡)를 설정하여 식생을 조사하였다. 식물군집을 분류한 결과 16개 조사구는 신갈나무 혼효림 군집, 소나무-신갈나무 군집, 신갈나무 우점군집의 3개 군집으로 분류되었다. 북한산 국립공원내 북한산성에서 인수봉에 이르는 지역의 우점수종은 신갈나무로 나타났다. 수종간의 상관관계는 신갈나무와 진달래, 신갈나무와 개옻나무, 진달래와 철쭉, 노린재나무와 철쭉, 당단풍과 함박꽃나무 등의 수종들 간에는 높은 정의상관이 인정 되었고, 물푸레나무와 진달래, 노간주나무와 당단풍, 쪽동백나무와 당단풍 등의 수종들간에 부의 상관이 비교적 높은 편이다. 조사지의 군집별 종 다양성도는 1.23 6~1.319의 범위로 다른 국립공원들의 능선부 식생에 비하여 비교적 높게 나타났다. To investigate the vegetation structure of the region from Bukhansanseong to Insubong in Bukhansan National park, 16 plost(2500㎡) set up with random sampling method were surveyed. Three groups Quercus mongolica -Mixed Broad Ieaved community, Pinus densiflora -Quercus mongolica community, Quercus mongolica community was classified (or communities were classified) by cluster analysis. Quercus mongolica were found as a major woody plant species in Bukhansan National park region. High positive correlation were proved between Quercus mongolica and Rhododendron; Quercus mongolica and Rhus tricocarpa; Rhododendron mucronulatum and Rhododendron schlippenbachi; Symplocos chinensis and Rhododendron schlippenbachii; Acer pseudosieboldianum and Magnolia sieboldii, and relatively high negative correlation was proved between Fraxinus rhynchophylla and Rhododendron mucronulatum; Juniperus rigida and Acer pseudosieboldianum; Styrax obassia and Acer pseudosieboldianum. Species diversity(H`) of investigated groups were ranged from 1.236~1.319 and it was relatively high compared to those of the ridge area of other national parks.

Cd_1-xMn_xTe 단결정의 광학적 특성

박효열,진광수,유병길,주정진,김중환 동의대학교 기초과학연구소 1994 基礎科學硏究論文集 Vol.4 No.1

Cd_1-xMn_xTe single crystals were grown by vertical Bridgman method and Mn mole fraction x was determined by the X-ray diffraction. Band gap and optical transition energies E₁, E₁+ △₁, E₂, △₁as Mn mole fraction x were obtained by measuring optical absorption and reflection at UV-visible region respectively. The change of the valence band spin-orbit splitting △₁at △ point of the Brillouin zone was discussed.

호장근 추출물의 Helicobacter pylori에 대한 생육억제 및 인간 위암세포주에 대한 유전적 손상 유도

박정현,김현정,이지원,임효권,이인선 대한암예방학회 2003 Journal of cancer prevention Vol.8 No.2

미세혈관 협심증 환자의 임상상 및 지표에 관한 연구

박상호,온영근,김현정,이태훈,길효욱,김현건,박수진,송해정,한대희,방덕원,신원용,이내희,김성구,권영주 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background : Microvascular angina or syndrom X is heterogenous and encompasses different pathogenetic entities. Several studies suggest that microvascular angina have good prognosis and occurs predominantly in postmenopausal women. Lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and fibrinogen are the independent risk factor of acute coronary syndrome and are the chief underlying cause of atherosclerosis. We investigated clinical characteristics and markers in patients with microvascular angina. Methods : We studies 23 patients diagnosed with microvascular angina and 16 control group with negative treadmill exercise test and no significant coronary angiogram. We investigated clinical characteristics in patients group and evaluated symptom during a follow-up period of 4.2± 2.5 month. Exercise time in treadmill exercise test was compared between patients and control group. We assessed the level of markers, such as lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and ESR in two groups. Results : There is no difference in age between two groups. Patients group had a tendency to increase in female, hypertension, DM, and smoking history, but there was no statistical significance difference. Exercise duration time was significantly decreased in patients with microvascular angina (p<0,05). Serum lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholestrol, Triglyceride, ESR, Fibrinogen and Homocystein value were no significant difference between microvascular angina and control group. Only CRP was significantly high in patients with microvascular angina (p<0.05). Conclusion : There was no statistical significance difference in age, sex, hypertension, DM and smoking history between two groups but patients group had a tendency to increase the prevalence in hypertension, DM and smoking history, female. Also, Symptom improvement and disappearance in follow-up was presented in 11 patients of 19(57.9%). Exercise tolerance was worse in patients group than control group. CRP value was significantly higher in patients group than control group. There was no statistical significance difference in lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, ESR and Fibrinogen between two groups.

체중감량 프로그램에 따른 체력의 변화에 대한 연구 : by Obese Female College Students in Seoul

박효정,장도석,장은재 동덕여자대학교 2003 생활과학연구 Vol.8 No.-

The purpose of this study was to investigate the effect of the aerobic exercise with calorie restricted diet on the change of the body composition, health-related fitness and performance-related fitness during 4 weeks. Twelve obese female college students ages from 20 to 24 who participated in this program. Variables were body composition; Fat mass, Fat free mass. Fat percentage, PIBW(Percentage of ideal Body Weight), BMI(Body Mass Index), health-related fitness and performance-related fitness. The results were as following; First. Diet was found to have a significant decrease of 266㎉ in the change of energy intake and exercise was found to have a significant consume of 309.71㎉ in the change of energy expenditure. Second, Weight, BMI, PIBW, percent body fat, fat mass were found to have a significant decrease of 5.02%, 5.01%, 5.25%, 2.58%, and 2.61% in the change of the body composition, respectively. (P>0.05) FFM was also decreased but it was not significantly. Third, Grip strength and Sit-ups were found to have a significant increase of 2.69% and 2.78% in the change of health-related fitness. (P>0.05) Back strength, Sitting trunk flexion and VO2max were found to have increase of 1.47%, 0.35% and 0.75% in the change of health-related fitness. Forth, Whole body reaction time was found to have a significant decrease of 2.62% in the change of performance-related fitness. Vertical jump and Closed eyes-foot balance were found to have a increase of 1.96% and 1.79% in the change of performance-related fitness. As the results, the weight control program demonstrated to have a positive effect in the improvement of the body composition, health-related fitness and performance-related fitness in obese female.

다중지능이론을 활용한 프로젝트학습이 초등학생의 학습동기와 사회과 학업성취에 미치는 영향

박성희,허효정 한국교육방법학회 2009 교육방법연구 Vol.21 No.2

The purpose of this research was to examine the effect of project-based learning using multiple intelligence theory on motivation and achievement of social science in elementary school. Ninety-five students of 4th grade in D elementary school participated in the study. The treatment were conducted for 4 weeks and data of pre-post tests with motivation and achievement test were collected. First, the result of ANCOVA with total score of motivation and the results of MANCOVA with sub-factors of motivation showed statistical significance. Besides the results of follow-up univariate F tests showed that the experiment group showed higher scores in the two sub-factors; Intrinsic and continuous motivation. Second, the results of ANCOVA using individual achievement score showed that the experiment group got higher scores than the control group with statistical significance. Besides the result of Mann-Whitney U test of team achievement score showed that the experiment group got higher scores than the control group, specifically in the area of creativity, relativeness, and logic.