Saccharomyces cerevisiae에서 Trichoderma Endoglucanase의 발현과 분비

남수완,김병우,신동하,김재범,신지원,정대균,정춘수 동의대학교 기초과학연구소 1999 基礎科學硏究論文集 Vol.9 No.1

The endoglucanase gene, egl6, of Trichoderma sp. was connected with the yeast ADH1 promoter, and the resultant plasmid, pVT-C4, was introduced into three S. cerevisiae host strains (YNN27, 2805, and SEY2102). Among each 80 transformants, the cell growth and expression level of endoglucanase were compared in test-tube cultivation, and three respective transformants for each host cells showing the highest expression level and cell growth were selected. When three recombinant yeast cells were batchwise cultivated for 48 hr in flask, the total activities of endoglucanase expressed were about 1140 unit/1 with 2805/pVT-C4, 1020 unit/l with SEY2102/pVT-C4, and 590 unit/l with (YNN27/pVT-C4. Irrespective of host strain, about 80% of the expressed endoglucanase was detected in the extracellular medium. In addition, it was also found that the recombinant enzyme was secreted into the culture medium as two major forms of lightly and heavily glycosylated proteins.

재조합 Saccharomyces cerevisiae에서 Inulinase와 Invertase의 발현과 분비에 미치는 배양조건의 영향

남수완,신동하,김연희 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

The effects of medium pH and culture temperature on the expression and secretion and secretion of inulinase and invertase were investigated with recombinant Saccharomyces cerevisiae cells. These cells were obtained by transformation of 2μ-based plasmids pYI10 and pYS10 which contain Kluyveromyces marxianus inulinase gene (INU1A) and S. cerevisiae invertase gene (SUC2), respectively, in the downstream of GAL1 promoter. The expression level and localization of inulinase and invertase were not affected significantly by the initial medium pH: secretion efficiencies of inulinase and invertase into the medium were about 90% and 60%, respectively, in the pH ranges of 4.0 to 6.5. However, the expression and secretion of both enzymes were strongly dependent on the culture temperature. The highest expression (7.7 units/mL) and secretion (6.7 units/mL) of inulinase were observed at 28℃ and 30℃. As a consequence of decreased localization of inulinase in the periplasmic space, the secretion efficiency increased from 68% at 20℃ to 95% at 35℃. The total expression level and secretion efficiency of invertase increased from 19 units/mL and 55% at 20℃ to 25 units/mL and 68% at 35℃, respectively. Irrespective of the culture temperature, the invertase activity in the cellular fraction (periplasmic space and cytoplasmic fractions) was kept constant at around 33∼45%.

전치부 개교를 동반한 골격성 Ⅲ급 부정교합 환자의 악교정 수술 후 교합평면의 변화와 안정성에 관한 연구

신수정,황병남,이정근,이승훈 대한치과교정학회 1999 대한치과교정학회지 Vol.29 No.1

악골의 골격적 부조화를 가진 환자의 교정치료는 악교정 수술을 필요로 하고, 악교정 수술에 의해 교합평면각은 변화된다. 특히 전치부 개교를 동반한 III급 부정교합 환자에서 치아안면 기형을 치료하기 위한 교합평면각의 변화에 대하여 많은 논란이 있어 왔다. 교합평면각을 증가시키는 하악골의 시계 방향 회전(clockwise rotation)은 전치부 개교에 대한 적절한 치료법으로 추천되어 왔고 하악골의 반시계 방향의 회전(counterclockwise rotation)은 하악지 고경을 증가시키는 방향으로 하악골을 회전시킨다는 개념으로 인해 재발(relapse)을 유발하는 불안정한 수술 방법으로 인식되어 왔다. 본 연구는 전치부 개교를 동반한 골격성 Ⅲ급 부정교합 환자에서 교합평면의 반시계 방향회전을 동반한 하악지시상분할 골절단술 시행 후 교합평면각의 변화와 술후 안정성과의 관계를 평가하고자 하였다. 하악지 시상분할 골절단술과 rigid fixation으로 치료받은 환자 25명(평균연령 20.6세)을 대상으로 하여 수술 작전(T1), 술후 2주내(T2), 그리고 술후 6개월 이후(T3)에 촬영한 측모 두부계측 방사선 사진을 통계분석한 결과 (Paired t-test, Pearson correlation analysis), 다음의 결론을 얻었다. 1. 악교정 수술 후 (T2) 하악평면각은 2.9° 감소하였고 SN평면에 대한 하악 교합평면의 각도는 2.7°감소하였다. 술후 6개월경과 후 (T3)후안면 고경의 감소로 인해(P<0.01) 하악 평면각은 1.0° 증가하였으나 하악 교합평면의 경사도는 변화되지 않았다. 2. 악교정 수술 후 시간 경과(T3)에 의해 발생한 수평적 개발은 하악골의 전방 이동량이 B점에서 1.6mm로 수술시 전체 후방 이동량의 약 22%였다. 전안면 고경에서는 수직적 재발이 발생하지 않았으나, 후안면 고경은 감소하였다(P<0.01). 3. 수평적 재발과 상관관계를 보이는 항목은 하악평면각 이었고(P<0.01) 수술 6개월경과 후 후안면 고경의 감소와 상관관계가 있는 요소로는 수술시 하악골의 후방 이동량(P<0.01), 하악지 고경의 증가량(P<0.01), 그리고 하악평면각의 감소량(P<0.01) 등이 있었다. 4. 수술시 하악 교합평면 경사도의 변화량과 술후 재발과는 상관관계가 없었다. The purpose of this study is to investigate the stability of counterclockwise rotation of mandible by sagittal split ramus osteotomy to correct the skeletal Class Ⅲ malocclusion with anterior open bite. Twenty five skeletal Class Ⅲ open bite patients(mean age 20.6 years) who were treated by the sagittal split ramus osteotomies with rigid fixation were examined in this study. Cephalometric radiographs were taken for each patients preoperative (T1), early postoperative(T2), and late postoperative period(T3). Mean postoperative period was 8.0 months. Cephalometric analysis was done and data from T1, T2, T3 were analyzed statistically by Paired t-test and Pearson correlation analysis. The following results were obtained. 1. Mandibular plane angle decreased 2.9° and mandibular occlusal plane angle related to SNplane decreased 2.7° after orthognathic surgery(T2). At 6 months after orthognathic surgery(T3), mandibular plane angle increased 1.0°, but mandibular occlusal plane angle did not changed. 2. The amount of horizontal relapse long time after orthognathic surgery(T3) was 1.6mm at B point and it was 22% of the total posterior movements. There was no vertical relapse in the anterior facial height. 3. The related factor with horizontal relapse at late postoperative period was mandibular plane angle(p<0.01). The related factors with decreasing posterior facial height were amount of mandibular setback(p<0.01), increasing of mandibular ramus height(p<0.01), and decrease of the mandibular plane angle during operation(p<0.01). 4. There was no relationship between the amount of changes in mandibular occlusal plan angle during operation and the amount of relapse after surgery.

기관지천식 환자에서 유도객담을 이용한 기도 염증의 평가

남언정,이종명,김건우,김신우,김능수 경북대학교 병원 2003 경북대학교병원의학연구소논문집 Vol.7 No.1

Objectives : This study was performed to investigate the relationship between cell counts, supernatant and lysate ECP levels in sputum, and physiologic markers in adult asthmatic. Methods : Twenty-two patients with mild to moderate persistent asthma, ten patients with acute exacerbated asthma and nine healthy subjects were enrolled. Sputum was induced by inhalation of hypertonic saline, and homogenized with 0.1% dithiothreitol. A total and differential cell was measured in supernatant fluid. Cell pellet was centrifuged, and ECP(supernatant ECP) was measured in supernatant fluid. Cell pellet was reacted with a cellular lysis buffer to release cell-associated ECP, and ECP(lysate ECP) was measured again in supernatant fluid. The ratio of supernatant to lysate ECP was calculated as an index of eosinophil degranulation. Spirometry and methacholine bronchial challenge tests were also performed as physiological markers of asthma. Results : The patients with acute exacerbated asthma showed significantly higher percentage of sputum neutrophil, eosinophil count, concentration of sputum supernatant ECP and ratio of supernatant to lysate ECP than those of normal controls and stable asthmatic patients(p<0.05, respectively). The level of sputum supernatant ECP, supernatant/lysate ECP ratio, and percentage of neutrophil showed negative correlations with pulmonary functions, but no correlations with a degree of bronchial hyperresponsiveness. There was no significant correlations between of serum ECP level and physiological parameters. Conclusion : These results suggest that both neutrophils and eosinophils play roles in the exacerbation of asthma. The sputum supernatant/lysate ECP ratio might be valuable in assessment of activation status of eosinophils in various hypereosinophilic conditions or diseases.

어린이의 꿈, 공상 및 희망에 대한 경험적 연구(1) : 발달학적 관점에서 고찰 FROM DEVELOPMENTAL PERSPECTIVE

김수정,신민섭,남 민,홍강의 대한소아청소년정신의학회 1997 소아청소년정신의학 Vol.8 No.1

저자들은 서울 시내에 위치한 2개 초등학교 1,3,5학년 학생 273명과 그들의 부모를 대상으로 하여 어린이의 꿈, 공상, 장래 희망 및 평소 상태를 경험적으로 조사하여, 발달학적 관점에서 이들의 특성 변화를 알아보고, 이들간의 상호 연관성을 살펴보았다. 그리고 이를 선행 연구와 비교하여 다음과 같은 결과를 얻었다. 1)어린이의 인지 발달 및 사회화와 더불어 꿈은 발달한다. 즉, 꿈에 동물, 가족 성원, 아는 사람의 출현은 줄고 나 자신, 동성 친구, 모르는 사람의 출현이 증가하며, 보다 현실적이고 정교한 내용으로 발달하였는데 이는 선행 연구에서와 비슷한 결과로 보여진다. 2)공상, 장래 희망, 그리고 평소 상태 역시 어린이의 학년에 따라 변화를 보였다. 즉 공상의 빈도는 학년에 따라 증가하였으며, 공상 내용 및 장래 희망도 보다 자기 중심적이고 현실적인 내용으로 변화하였다. 평소 상태 역시 학년에 따라 변화하였는데, 시험이나 공부에 대한 중압감이 심한 우리 나라의 현 실정을 반영하는 소견을 보였다. 3)꿈, 공상, 장래 희망, 그리고 평소 상태는 상호 연관성을 보였다. 즉, 기분 상태에서는 꿈과 공상, 공상과 평소 상태 사이에 유의한 연관성이 있었다. 또한 행복한 기분 및 무서운 기분은 각각의 기분과 합치되는 꿈, 공상, 장래 희망의 내용과 정적 연관성을 보였다. 내용에 있어서는 같은 내용의 꿈과 공상은 상당 부분 정적 연관성을 보였다. 본 연구는 정상 어린이들의 꿈, 공상, 장래 희망의 특성 변화를 발달학적 측면에서 살펴보고자 한 국내 최초의 시도로서 의의를 가지고 있다. 본 연구 결과를 통해 저자들은 꿈과 공상은 현실-모방-놀이-공상-꿈의 연속선상에 있으며 서로 밀접한 연관성을 가지고 나타남을 입증할 수 있었다. 마지막으로 앞에서 언급한 바와 같이 근래의 꿈 연구자들은 꿈의 심리적 의의에 대하여 서로 상반된 견해를 보이고 있으나, 본 연구 결과에서 꿈이 학년에 따라 특성이 변화한 점, 의미있는 감정 체험에 대한 반응성을 보인 점, 그리고 공상이나 장래 희망과 같은 각성 시의 사고 및 평소 상태와 밀접한 연관성을 보인 점 등은 꿈의 심리적 의의를 지지하는 소견으로 볼 수 있겠다. We examined the developmental characteristics of dream, daydream, and hope of elementary school students in their lst, 3rd, and 5th grades, and studied the correlation between three variables. We summarized the results of this study as follows. 1) The contents of dream developed with the cognitive development socialization process. In detail, appearance of animals, family members and known persons gradually decreased, and appearance of self, same-sex peers, and strangers gradually increased. These results are comparable with the previous studies of dream. 2) Daydream, hope, and current state also developed with age. In detail, frequency of daydream increased, and the contents of daydream and hope hanged toward more self-centered and more realistic. The changes of current state are thought to reflect the specific situation of Korea. 3) There are some correlation among dream, daydream, and hope. Especially happy mood and fearful mood had positive correlation with the relevant contents of dream, daydream, and hope. Also the contents of dream showed positive correlation with the same contents of daydream. This study is the first trial which investigate the developmental characteristics of dream, daydream, and hope of normal children in Korea. We verified that dream and daydream are on the continuous line of 'reality-imitation-play-daydream-dream'. Finally, considering the facts that dream developed with age ; responded to meaningful emotional experiences ; and had the correlation with daydream, hope and current state, the results of this study support the psychological meaning of dream.

Taxol합성에 관한 연구. Ⅱ : 3-(2-Hydroxyethyl)-2, 2, 4-trimethyl-3-cyclohexenone SEM ether의 합성

임남수,신동수,정윤성,김정주,박두천,주우홍 昌原大學校 基礎科學硏究所 1995 基礎科學硏究所論文集 Vol.7 No.-

3-(2-Hydroxyethyl)-2,2,4-trimethyl-3-cyclohexenone SEM ether ??, an intermediate in the synthesis of taxol was provided in eight steps. The keto ketal ?? was obtained in three steps from 1,3-cyclohexanedione. Reaction of the potassium enolate of ?? with N-phenyltrifluoromethanesulfonimide provided triflate ?? in 84% yield. Thus palladium-mediated cross-coupling reaction of enol triflate ?? with vinyltributylstannane afforded diene ?? in a 89% yield. 3-(2-Hydroxyethyl)-2,2,4-trimethyl-3-Cyclohexenone SEM ether was prepared from diene ?? by sequential hydroboration [9-BBN, THF, 89%], deprotection[pTsOH ·H₂O, THF-H₂O,~100%], and silyation [SEMC], iPr₂NEt, nBu₄NI, CH₂Cl₂, 94%]

Enterobacter cloacae에 의한 Cd(Ⅱ) 제거

백종수,원성호,김장규,신상규,김남기 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.1

In order to obtain the basic data for the optimum condition to remove Cd(Ⅱ) biologically, the variation effect of Cd(Ⅱ) concentration and stirring rate on the removal of Cd(Ⅱ) were investigated by Enterobacter cloacae(ATCC 13047). The initial con-centrations of Cd(Ⅱ) were changed 10, 50, 100, and 500 ppm and the stirring rates 100, 150, and 200rpm. The initial pH and experimental temperature were fixed at pH 6.8 and 30℃. The results were obtained as follows : 1) The minimum growth inhibitorn ratio of Enterobacter cloacae was 42% at 10ppm of Cd (Ⅱ) and stirring rate 200rpm. 2) The maximum removal ratio of Cd(Ⅱ) was 8.6% at 200rpm and 50ppm of Cd(Ⅱ) by Enterobacter cloacae.

비강에 발생한 반전성 유두종 1예

도남용,김왕수,박성웅,신태호,전호종,박영진 대한이비인후과학회 1981 대한이비인후과학회지 두경부외과학 Vol.24 No.4

Wolff-Parkinson-White증후군의 임상상 및 전기생리학적 소견

안신기,이문형,장양수,오동진,최인석,하종원,임세중,김병옥,정남식,심원흠,조승연,김성순 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.9

하천제방 根固工크기에 관한 실험연구

文秀男,申文燮 群山大學校 1996 論文集 Vol.23 No.-

An experimental study on straight channel that belongs to steep slope at medium and upper stream solved tractive force from real gauged stage and velocity by hydraulic modeling test. And inducing genearlized Cross Type Flat Concrete Block Foot Protection Work Size in scouring point. The objectives of this experimental study is to provide Foot Protection Work Size for the sake of effective uses for the bank stabilization. And use for data of channel improvement some anticipated scale of foot protection work and so on.