Effects of pyridoxine on a high‐fat diet‐induced reduction of cell proliferation and neuroblast differentiation depend on cyclic adenosine monophosphate response element binding protein in the mouse dentate gyrus

Yoo, Dae Young,Kim, Woosuk,Yoo, Ki‐,Yeon,Nam, Sung Min,Chung, Jin Young,Yoon, Yeo Sung,Won, Moo‐,Ho,Hwang, In Koo Wiley Subscription Services, Inc., A Wiley Company 2012 JOURNAL OF NEUROSCIENCE RESEARCH - Vol.90 No.8

<P><B>Abstract</B></P><P>In this study, we challenged pyridoxine to mice fed a high‐fat diet (HFD) and investigated the effects of pyridoxine on HFD‐induced phenotypes such as blood glucose, reduction of cell proliferation and neuroblast differentiation in the dentate gyrus using Ki67 and doublecortin (DCX), respectively. Mice were fed a commercially available low‐fat diet (LFD) as control diet or HFD (60% fat) for 8 weeks. After 5 weeks of LFD or HFD treatment, 350 mg/kg pyridoxine was administered for 3 weeks. The administration of pyridoxine significantly decreased body weight in the HFD‐treated group. In addition, there were no significant differences in hepatic histology and pancreatic insulin‐immunoreactive (‐ir) and glucagon‐ir cells of the HFD‐treated group after pyridoxine treatment. In the HFD‐fed group, Ki67‐positive nuclei and DCX‐ir neuroblasts were significantly decreased in the dentate gyrus compared with those in the LFD‐fed mice. However, the administration of pyridoxine significantly increased Ki67‐positive nuclei and DCX‐ir neuroblasts in the dentate gyrus in both LFD‐ and HFD‐fed mice. In addition, the administration of pyridoxine significantly increased the protein levels of glutamic acid decarboxylase 67 (GAD67) and brain‐derived neurotrophic factor (BDNF) and the immunoreactivity of phosphorylated cyclic AMP response element binding protein (pCREB) compared with the vehicle‐treated LFD‐ and HFD‐fed mice. In contrast, the administration of pyridoxine significantly decreased HFD‐induced malondialdehyde (MDA) levels in the hippocampus. These results showed that pyridoxine supplement reduced the HFD‐induced reduction of cell proliferation and neuroblast differentiation in the dentate gyrus via controlling the levels of GAD67, pCREB, BDNF, and MDA. © 2012 Wiley Periodicals, Inc.</P>

Focal adhesion kinase and src expression in premalignant and malignant skin lesions

( Ju Yeon Choi ),( Jae Yun Lim ),( Han Saem Kim ),( Jung Min ),( Jung In Kim ),( Hyun Min Seo ),( Sang Hyeon Hwang ),( Chong Won Choi ),( Yoon Hwan Kim ),( Jin Hee Sohn ),( Hyunjoo Lee ),( Won Serk Ki 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Focal adhesion kinase (FAK) and Src are non-receptor tyrosine kinases. FAK and Src play a critical role in inducing malignant transformation in tumor cells. Objectives: We performed immunohistochemical staining for total and phosphorylated forms of FAK and Src, to evaluate the role of FAK and Src in the development of premalignant and malignant skin lesions. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Results: Cells positive for t-Src, p-Src-y530, t-FAK and pFAK-s722 were detected in premalignant intra-epithelial lesions (PELs) and squamous cell carcinomas (SCCs), but not in the perilesional skin. There was a tendency towards high correlation between Ki-67 and t-FAK or pFAK-s722, suggestive of the active role of FAK in cell proliferation. Conclusion: Our findings of higher t-Src and p-Src-y530 positive cells in PELs, as compared to SCCs (with higher Ki-67 level), are suggestive of the other role of Src in tumor formation and progression, which requires further investigation.

Cone beam형 전산화 단층촬영장치를 이용한 설공의 해부학적 구조

기민우,황의환,이상래 대한구강악안면방사선학회 2004 Imaging Science in Dentistry Vol.34 No.3

Purpose : To evaluate whether cone beam computed tomography can depict the distribution, position, frequency, relative vertical dimension, and the diameter of the lingual foramen and direction of lingual bone canal. Materials and Methods : Cone beam computed tomography of mandible was performed on 25 males and 25 females with no history of any orthodontic treatments or any other dental surgeries. A statistical comparison was done on the mean values of males and females. Results : In the location and distribution of lingual foramina, median lingual foramen was found in all subjects and lateral lingual foramen in 58%. In the lateral lingual foramen, bilateral type was found in 28% and unilateral type in 30%. In the number of lingual foramina, median lingual foramen had two foramina and lateral lingual foramen had one foramen, mostly. In the relative mean vertical dimension of lingual foramina, median lingual foramen was 0.03 ±0.08, and both lateral lingual foramina was 0.20±0.04. The mean diameter of lingual foramina, median lingual foramen was 0.9mm±0.28, right lateral lingual foramen was 0.92mm±0.23, and left lateral lingual foramen was 0.88 mm±0.27. The most frequent direction of the lingual bone canals, median lingual bone canal proceeded in anteroinferior direction and lateral lingual bone canal in anterosuperolateral direction. Conclusion : Cone beam computed tomography can be helpful for surgery and implantation on the mandibular area. Radiologist should be aware of this anatomical feature and its possible implications.

Ipriflavone 투여가 백서의 실험적 치아이동 후 치주조직의 재형성에 미치는 영향

민지현,조진형,이기헌,황현식 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.5

본 연구는 치아이동 후 ipriflavone 투여가 치주조직의 재형성, 나아가 치아회귀 억제에 미치는 영향을 알아보기 위하여 시행되었다. 웅성 백서 72마리를 대상으로 상악 좌우측 제1, 2구치 사이에 교정용 고무밴드를 끼워 제1, 2구치를 3주간 이동시킨 다음, 교정용 고무밴드 제거 1일 전부터 매일 체중 kg당 50 mg 또는 400 mg의 ipriflavone을 투여한 실험군과 ipriflavone을 투여하지 않은 대조군으로 구분하였다. 교정용 고무밴드를 제거함으로써 회귀가 나타나기 시작한 날로부터 5일 간격으로 15일까지 각각 실험동물을 희생시켜가며 치아회귀 거리를 계측하는 한편 치주조직 재형성 소견을 관찰하였다. Ipriflavone을 투여하고 치아회귀를 관찰한 결과 용량에 관계없이 대조군에 비하여 적은 양의 회귀를 나타내었으며 시간 간격에 따른 치아회귀를 관찰한 결과 대부분의 회귀가 5일째 나타난 대조군과 달리, 늦게 그리고 점진적으로 나타나는 양상을 보였다. Ipriflavone을 투여하고 조직소견을 관찰한 결과 대조군에 비하여 치조골연이 매끄러운 양상을 보였으며 시간 간격에 따른 조직소견을 관찰한 결과 대조군에 비하여 치주인대 재배열이 빠르게 나타나는 양상을 보였다. 이상의 결과는 ipriflavone의 투여가 치주조직의 재형성을 통하여 회귀를 억제함으로 교정적 치아이동 후 보정에 도움이 될 수 있음을 시사하였다. Objective: The purpose of this study was to examine the effect of ipriflavone on periodontal reorganization and prevention of relapse following tooth movement. Methods: Orthodontic rubber bands were inserted between the first and second maxillary molars of 27 white male rats for 3 weeks for experimental tooth movement. From one day before the removal of orthodontic rubber band, ipriflavone was administered 50 or 400 mg/kg daily in each experimental group whereas carboxymethyl cellulose solution was administered in the control group. They were sacrificed at the 5, 10, and 15th day from the day of removal of orthodontic rubber bands. The amount of relapse was evaluated by measuring the interdental space, and the extent of periodontal reorganization was compared through histological examination. Results: In case of ipriflavone administration, the amount and velocity of relapse was less and slower compared to the control group. In addition, the ipriflavone group showed more rapid periodontal reorganization compared to the control group. Conclusions: The results of the present study suggest that ipriflavone administration can be used effectively in the prevention of relapse following orthodontic tooth movement through the acceleration of periodontal reorganization.

운동장애형 기능성 소화불량증 환자에서의 식도내압검사 성적

황일란,김진홍,이광재,김정민,함기백,조성원 대한소화관운동학회 1998 Journal of Neurogastroenterology and Motility (JNM Vol.4 No.1

아리스톨로크산 함유 생약제에 대한 안전성평가연구 : 3개월간 반복투여독성시험을 통한 신장독성평가

황명실,박미선,문지영,이지선,염영나,이효민,신동환,강진석,윤은경,최미나,육미영,장동덕,길광섭,김승희,양기화 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

본 실험에서는 아리스톨로크산을 함운하고 있는 생약제중 하나인 마두령(.4risfoforfioe JTurruf)의 90일 반복투여독성시헌을 실f,」하였다. 마두령에서 아리스톨로크산을 정량분석한 결과 마두령 단일 건조븐말의 경운 2.112mg/g, 잉상에서 처방되고 있는 마든령복방 (마두령외 9가지 생약제 복합처방) 건조분말꼭 경무에는 0.066m9/5으로 각각 검출되었다. 본 실험에서 용량설정은 마두령 처리군 (저용량 군; 21.3m9/k9/day (임상용량), 중용량군; 2t3mgfg/day, 고용량군; 2430mg딘g b.w./day), 마두령복방 처리군 (427mg/kg b.w./da?; 임상용량) 및 positive control (아리스톨로크산) 처리군 (저용량군;0.05mgag/da17, 중용량군; 0.Smg/kg/day, 고용량군; 5mg./kg/dal·1으료 하여 랫드에게 경구투여하였다. 마두령단방 처궈근 및 마두령복방 처리군에서 저용량군과 중응량군에서는 잉상관찰 및 생화학적 분석에서 패조군과 유의적인 차이가 없었다. 그러나 마두령단방 고용량근에서는 간, 신장의 상대적인 무계가 증가되었고, 신장의 유두이행상피증식 및 암종이 관찰되었으며, 위에서는 편평세포암종이 관찰되었다. 하자만 간손상이나 간암은 유발하지는 않는 것으로 판단되었다. 결론끌으로 본 실험의 결과에서는 아리스톨로크산 함유 생약제인 마두령이 임상용량인 저응량 처리군에서는 독성을 나타내지 않았으나, 고용쏭으로 장기 복용시켰을 깅우 설치류의 전위부위 및 신장에서 독성이 있음을 알 수 있었다. Chines herbs nephropathy (CHN) has been described in young women who had taken a slimming pills containing some chines herbs. Aristolochic acid (AA), suspected substance as the causal factor of CHN, is known a carcinogen. The Aristolochiae fructus (fruit of Aristolochia contorta) was used in Korean Traditional Medicine consists of appropriate amounts of mixed natural products. Subchronic toxicity of A. furctus containing aristolochic acid was investigated in SD rats. The body weight and clinical signs were observed after orally administration of A. futctus at doses of 21.2, 213, and 2130 mg/kg/day; mixture (including 9 other herbs) at dose of 427 mg/kg/day; aristolochic aicd (Ⅰ+Ⅱ) at doses of 0.05, 0.5, and 5 mg/kg/day for 3 months. At the end of the treatment, high dose treated animals revealed a deficit in final body weight about 25% compared with that of control. Organ weights of kidney, liver, testis, or ovary were increased with dose dependent manner. No changes considered to be due to the administration of A. furctus, mixture or aristolochic acid were in hematological and clinical study. However, significant changes at histopathological study of kidney and stomach were boserved in high-dose treated groups. In conclusion, for human health safety it needs appropriate regulatory actions regarding the use of natural herbal medicines known or suspected of containing aristolochic acid.

Atactic Polypropylene에 대한 염회비닐의 그라프트 중합

황택성,민병철,맹기석 忠南大學校 産業技術硏究所 1987 산업기술연구논문집 Vol.2 No.2

Atactic Polypropylene, a byproduct obtained in the manufacture of isotatic polypropylene, has been synthesed under various reaction conditions. In this study, suspension graft polymerizations of Vinylchloride onto Atatic polypropylene were carried out in aqueous with benzoyl peroxide as initiator. The effect on the grafting efficiency and reaction rate was observed by varing APP concentration Also, reactivity onto each hydrogen is the most good tertiary hydrogen among primary, secondary and tertiary. If the vinyl chloride concentration contains small in the polymerization, graftmer have carbonyl group, abnomal structure.

先秦儒學의 根本問題 : 「中」 思想과 關聯하여

閔晃基 혜전대학 1999 出版文化硏究所論文集 Vol.1 No.-

치과용 금합금의 표면처리에 따른 교정용 브라켓의 전단결합강도 변화

민지현,황현식,이기헌,박병주 전남대학교 치과대학 2000 전남치대논문집 Vol.12 No.1

A bracket bonding to the dental gold alloy shows a lower bond strength than the natural teeth, and this can be a possible source of subsequent bond failure. This study aims to evaluate the effect of various gold alloy surface treatment techniques on shear bond strength between the orthodontic adhesives and the gold alloy and to find ways of increasing the bond strength. Two hundred and forty specimens made of the dental gold alloy were divided into twelve groups based on the combination of surface treatment methods(non-surface treatment, sandblasted, sandblasted plus tin-plated, and sandblasted plus intermediate adhesive) and adhesive systems(Ortho-one, Panavia 21, Superbond C&B). The specimens with brackets bonded were placed in distilled water at 37℃ for 24 hours and shear bond strength was measured by a universal testing machine. The results were as follows: 1. All surface-treated groups showed a significantly higher shear bond strength than non-surface-treated groups. 2. The sandblasted plus tin-plated group showed a significantly higher shear bond strength than the sandblasted group only when Panavia 21 was involved. 3. The sandblasted plus intermediate adhesive group showed a significantly higher shear bond strength than sandblasted group regardless of the type of adhesive used. 4. Of the three resin adhesive types, the Superbond C&B showed the highest bond strength, followed by Panavia 21 and Ortho-one. These findings suggest that a combination of sandblasting and intermediate resin treatment is desirable in order to enhance bracket bond strength regardless of adhesive types.

2차원 방사선 규격사진에서 하악골 상행지 고경의 보정분석에 관한 3차원 CT 영상 연구

황현식,김형민,이기헌,임회정 대한치과교정학회 2007 대한치과교정학회지 Vol.37 No.5

안면비대칭 환자의 진단 및 치료계획 수립 시 하악골 상행지 고경의 좌우 차이를 정확히 판정하는 것이 필요하다. 본 연구는 2차원 방사선사진에서 정면 상행지경사도 및 측면 상행지경사도를 이용하여 하악골 상행지 고경을 보정하는 방법이 비대칭 판정에 도움이 되는지 알아보고자 시행되었다. 이부 편위가 있는 안면비대칭자 40명을 대상으로 정모두부방사선규격사진을 촬영하고 좌우 각각의 하악골 상행지 고경을 측정하는 한편 정모 및 측모 방사선사진에서 계측된 정면 상행지 경사도 및 측면 상행지 경사도를 이용하여 상행지 고경을 3차원적 개념으로 보정한 후, 실제 3차원 영상에서의 비대칭 판정 결과와 비교 분석을 시행하였다. 측정결과, 정면 및 측면 상행지 경사도는 이부편위측에 비하여 반대측에서 크게 나타났으며 보정 후 상행지 고경의 좌우 차이는 증가하는 양상을 나타내었다. 보정 전 그리고 보정 후 각각에서 상행지 고경의 좌우비대칭 여부를 판정한 후, 3차원 영상에서의 판정을 기준으로 하여 보정에 따른 sensitivity, specificity 그리고 accuracy 변화를 살펴본 결과, specificity는 44%에서 22%로 감소하였으나 sensitivity는 74%에서 94%로 증가하는 것으로 나타났다. 아울러 accuracy가 보정 전 68%에서 보정 후 78%로 증가하는 것으로 나타나 정면 및 측면 상행지 경사도를 이용하여 상행지 고경을 3차원적 개념으로 보정할 경우, 이는 보다 정확한 비대칭 판정에 유용함을 보여주었다. Objective: Defining right and left side differences in mandibular ramus height is one of the key elements in the diagnosis of facial asymmetry. The purpose of the present study was to evaluate the effect of correction of ramus height with frontal and lateral ramal inclinations (FRI and LRI) in 2-dimensional cephalograms and observe how this affects the diagnostic accuracy of asymmetry. Methods: Frontal and lateral cephalograms were obtained in 40 individuals with chin deviation. FRI and LRI were measured on each side and ramus height measurement was corrected with these inclinations using Pythagorean's theorem. The results of diagnosis before and after correction on cephalograms were compared with the results in 3D CT images. Results: Both FRI and LRI showed greater values in the contralateral side than in the chin-deviated side and these contributed to an increase in the right and left side ramus height differences. After comparison of diagnostic results before and after correction on cephalograms with the results on 3D CT images, the sensitivity increased significantly (from 74 to 94 %) whereas the specificity decreased (from 44 to 22 %). Overall accuracy increased from 68 to 78% with the correction using FRI and LRI. Conclusions: The results of the present study indicate that correction of ramus height with FRI and LRI is useful for an accurate diagnosis of facial asymmetry on frontal cephalograms.