다중 중합효소 연쇄반응을 이용한 소의 Johne병 진단 기법 확립

김종배,송혜원,김근희,김홍,신광순,김두 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.1

반추수에서 발생하는 Johne병의 조기 진단 방법을 제시하고 이 질병의 원인체와 미생물학적 특징이 유사한 M. bovis, M. avium 등의 mycobacteria감염증을 감별 진단하는 방법을 개발하기 위하여 Mycobacterium 균속의 표준균주를 사용하여 중합효소 연쇄반응을 확립하였다. Johne병으로 의심되는 소의 혈액과 유즙을 채취하여 분리한 단핵구 및 거식세포로부터 genomic DNA를 추출하였다. 각 시료로부터 추출한 DNA를 template로 이용하여 Mycobacterium spp.에 특이적인 16S rDNA primer set를 이용한 PCR을 수행하여 시료내의 mycobacterial DNA 보유 여부를 확인하였다. 한편 mycobacteria 양성으로 확인된 시료는 M. avium complex 균종에 특이한 16S rDNA 염기서열을 기초로하여 제작한 primer set와 M. paratuberculosis 의 IS900 sequence에 특이한 primer set를 이용하여 duplex PCR을 수행하여 Johne병 원인체의 보균 여부를 조사하였으며, 이 결과를 oligonucleotide probe를 사용한 Southern blot hybridization을 통하여 다시 확인하였다. 이와 같은 duplex PCR 기법을 실제 축산 현장에서 수집한 유즙과 말초혈액으로부터 분리한 단핵구 및 거식세포 시료에 적용한 결과 본 연구에서 확립한 duplex PCR기법 유용성을 확인할 수 있었다. Diagnosis of Bovine Johne's Disease Using Multiplex Polymerase Chain Reactions In order to improve the early diagnosis of Johne's disease in ruminants, duplex polymerase chain reaction system for the detection of the etiologic agent of M. paratuberculosis and for the differentiation of other mycobacterial animal pathogens, such as M. bovis and M. avium, was applied. Genomic DNAs were purified from peripheral blood monocytes or milk macrophages and were used as templates in the duplex PCR. Detection of Mycobacterium spp. in the specimen was carried out by PCR using primer set specific to the mycobacterial 16S rDNA. And then, mycobacterial DNA-positive specimens were further differentiated with duplex PCR system which was composed of primer sets specific to 16S rDNA of M. avium complex and IS900 gene of M. paratuberculosis. The results were re-confirmed by Southern blot hybridization with oligonucleotide specific to the internal sequence of IS900 PCR amplicons. The applicability of this duplex PCR system was evaluated with DNAs extracted from clinical specimens of peripheral blood monocytes and milk macrophages. In summary, the duplex PCR amplification system described in this experiment is promising molecular technique for the early diagnosis of Johne's disease in ruminants.

노인 환자에서 경피적 관동맥 중재술 후 조영제 유발 신부전의 발생률, 위험인자와 임상적 의의

김광일,배장환,강현재,채인호,김효수,손대원,김철호,오병희,이명묵,박영배,최윤식 대한노인병학회 2004 대한노인병학회지 Vol.8 No.1

항암화학요법을 받는 암 환자에 대한 Nucare^R의 영양지지효과

조덕연,김현수,곽상혁,강정현,김철희,배광봉,김종숙,박상준,윤환중,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Purpose : The purpose of this study was to evaluate the effect of nutritional support with enteral liquid supplement in cancer patients receving chemotherapy for possible benefit in nutritional, immunologic and golbal function of patients. Patients and Methods : From October 1995 to February 1997, 30 advanced cancer patients receving chemotherapy were divided two roups. The Nycare group, in addition to normal diet, Nucare^R enteral supplement was given for 1week right after chemotherapy for the duration of 2 chemotherapy cycles. Control group received only normal diet without parenteral fluid supplement for 2 chemotherapy cycles. Results : Median ages were 53 end 56 years for Nucare group and Contrl group respectively. Performance scores was less than 2 by ECOG scale. All patients were stage Ⅲ or Ⅳ. The physical parameters, such as weight, arm muscle circumference(AMC) and triceps skin fold(TSF) were decreased in both groups after 2 cycles of anticancer therapy. but it was less severe in Nucare group(p<0.05). Serum transferrin was maintained in mild deficit state in Nucare group, whereas it aggravated form mild to moderate deficit in Control group(p<0.05). Serum albumin level increased in Nucare group without statistical significance. but it decreased from normal to mild deficit in Control group. Serum total protein did not change significantly in Nucare group. but in Control group, serum total protein was decreased from 7.24±0.9 to 6.52±0.5(P<0.05). Total lymphocyte count did not change significantly in both groups. Conclusion : This study shows that the nutritional support with Nucare^R was effective in the prevention of nutritional deficit status in patients receving a nticancer chemotherapy.

단기배양을 통한 말초혈액 CD34 양성세포의 체외증폭

박상준,김철희,배광봉,김현수,김종숙,윤환중,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Background: It is suggested that clinical practice in the areas of bone marrow transplantation and gene therapy might rely on the ex vivo expansion of hematopoietic stem/progenitor cells. However, the condition for ex vivo expansion of hematopoietic progenitor cells is not well established. The authors pursued a series of experiments to define the proper conditions for the expansion of hematopoietic cells in the short-term liquid suspension culture of mobilized peripheral blood CD34+ cells. Methods: 1.0ml cultures were initiated with 9×10^3 PB CD34+ cells, which were isolated from PB mononuclear cells (MNCs) by high-gradient cell sorting, in 12 well plates with the various combinations of hematopoietic growth factors(HGF). The following recombinant human HGFs were used: stem cell factor(SCF) 100ng/ml, granulocyte colony-stimulating factor(G-CSF) 100ng/ml, GM-CSF(granulocyte, macrophage colony-stimulating factor) 100ng/ml, interleukin-1 beta(IL-1B) 1ng/ml, interleukin-3(IL-3) 20ng/ml, interleukin-6 (IL-6) 100ng/ml. At the end of culture, colony-forming cells were evaluated by semisolid clonogenic assay. Results: 1) Using the high-gradient magnetic sorting system, CD34^+ cells were isolated with a yield of 40 3% 2) In 7 day culture of PB CD34^+ cells(9×10^3 cells), nucleated cells expanded mean 10×10^3(range, 9 to 20×10^3) with the addition of SCF alone, 35×10^3(range, 10 to 60×10^3) with SCF plus G-CSF plus GM-CSF, and 130×10^3(range, 40 to 300×10^3) with the combination of SCF, G-CSF, IL-1, IL-3, IL-6, GM-CSF. In 14 day culture, nucleated cells expanded 10×10^3 to 1,860×10^3 with combination of human hematopoietic growth factors. 3) In 10 day culture without medium change of PB CD34^+ cells, CFU-GM numbers expanded 16. 5 fold(range, 7 to 59 fold) with the addition of SCF plus G-CSF plus Il-1 plus IL-3, 31.3 fold(range, 20.5 to 101.1 fold) with the combination of SCF, G-CSF, IL-1, IL-3, GM-CSF. In 14 day culture with or without medium change of PB CD34^+ cells was inferior to 10 day culture for CFU-GM expansion. 4) There was no significant difference for CFU-GM expansion between five growth factors(SCF,G-CSF,IL-1,IL-3,GM-CSF) and six growth factors(five growth factors plus IL-6). Conclusion: The authors could confirm that short-term suspension culture of peripheral blood CD34+ cells could expand hematopoietic progenitor cells. Ten-day culture with medium change of CD34+ cells with the addition of five growth factors, i.e. SCF, G-CSF, IL-1B, IL-3, and GM-CSF, might be the most efficient in this system.

임대주택법 일부개정법률안의 문제

이순배(Lee, Sun-Bae),김재광(Kim, Jae-Kwang) 한국토지공법학회 2012 土地公法硏究 Vol.58 No.-

The bill, a partial amendment to the Housing Act, is submitted to the Land, Transport and Maritime Affairs Committee of the National Assembly at Congressman Kim Tae-ho’s proposal, with major support of tenants in Kim-hae area. It is argued that the amendment is written for the tenants. The amendment includes some new provisions and other partially amended provisions. The main contents are comprised of provisions regarding the recovery of the full amount of the National Housing Fund, the criteria for appraisal prices relating to the conversion of rental housing for sale and the upper limit, retrospective application of the criteria, approval by the head of a Si, the head of Gun, or the head of Gu of rental conditions, and relevant penal provisions. Those provisions are for lowering the appraisal prices relating to the conversion of rental housing for sale by changing the criteria and ultimately for promoting tenants who reside in public rental housing to own a home. The main point is to retrospectively apply the same criteria for appraisal prices relating to the conversion of rental housing for sale to ten-year public rental housing units, as applied to five-year public rental housing units. Because the appraisal prices relating to the conversion of rental housing for sale directly affects interests between a landlord and a tenant after the tenancy is terminated, it is necessary to analyze the criteria for appraisal prices relating to the conversion of rental housing units for sale, the upper limit, and the timing for the application of the appraisal prices, in terms of the objectives of policies for rental housing and impartiality between landlords and tenants. Especially, after the simulation of the upper limit of appraisal prices relating to the conversion of rental housing for sale in an area such as Pan-gyo where housing prices had dramatically risen, the result turned out that the real prices relating to the conversion of rental housing for sale, if the conversion is made after the termination of ten year rental period, is projected to range from 160 percent to 200 percent of the housing prices (construction cost) at the time of the recruitment of occupants. In cases of ten year rental housing units, since the profits of rental business operators are guaranteed for a rental period by law, the housing prices seems to rise higher. The more housing prices rise in the relevant area, the prices for the conversion of rental housing for sale seems to rise higher. Thus, it turned out that such increase deprived tenants of the opportunity to own a home. Also, the deprivation was against the objectives of the policies for rental housing. 이 논문의 분석대상이 된 임대주택법의 일부개정법률안은 김해지역의 임차인들이 주축이 되어 김태호의원의 대표발의로 국회의 국토해양위원회에 제출되었던 법률안이다. 그래서 임차인들을 위한 개정안이라고 주장하며, 그 주요내용을 보면, 국민주택기금의 전액 환수, 분양전환가격의 산정기준과 상한선 및 소급적 적용, 시장ㆍ군수ㆍ구청장의 임대조건 승인, 임대조건의 승인 및 그 벌칙에 관한 것을 일부 신설ㆍ개정하는 것이다. 주요내용들 중에서 분양전환가격을 산정하는 방식의 변경을 통해 분양전환가격의 인하를 유도하고, 궁극적으로 공공임대주택에서 거주하는 임차인들의 내집마련을 도모하고자하는 것이라고 볼 수 있다. 그 핵심적인 내용은 현재 5년 임대주택에 적용되는 분양전환가격의 산정기준과 상한선을 10년 임대주택에 대하여 동일하게 적용하되, 소급적으로 적용하려는 것이 특징이다. 임대기간 만료 후 분양전환을 할 때 산정되는 분양전환가격의 크기는 임차인과 임대인간의 이해관계에 직접적으로 영향을 미치는 사항이기 때문에 분양전환가격의 산정기준과상한선 및 그 적용시기를 임대주택정책의 목적 및 임차인과 임대인 간의 형평성 등의 관점에서 분석할 필요가 있다. 특히 주택가격이 크게 상승한 (판교)지역에 대한 분양전환가격의 상한선을 시뮬레이션을 해본 결과, 10년 임대기간 만료 후 분양전환이 될 경우 예상되는 실질 분양전환가격은 최초 입주자 모집당시의 주택가격(건설원가)의 160%~200%에 달하는 것으로 나타났다. 10년 임대주택의 경우, 임대기간 중 임대사업자의 이윤까지 보전해주면서 해당 지역에서 주택가격이 상승하면 상승할수록 실질 분양전환가격의 크기는 더욱더 크게 증가함으로써 사실상 임차인들의 내집마련 기회가 박탈되고, 이것은 결국 임대주택정책의 목적에 반하는 것으로 나타났다.

경연 대회를 통한 초ㆍ중고생의 창의력 신장 효과에 관한 연구

이희복,육근철,류해일,김현섭,김희수,박달원,유병환,김선효,김여상,서광수,변두원,서명석,배성효,박종석,심규철,이성희 공주대학교 과학교육연구소 2001 과학교육연구 Vol.32 No.1

미래 사회를 대비하기 위한 개인의 개성을 존중과 창의적인 능력을 지닌 인간을 양성을 목적으로 충청남도 지역의 초 ㆍ 중고등학생들을 대상으로 한 창의력 경연대회가 공주대학교 과학교육연구소에서 개최되었다. 충청남도 초 ㆍ중 ㆍ 고등학생들을 대상으로 실시한 창의력 경연대회는 창조적 아이디어를 내어 스스로 문제를 해결해 나갈 수 있는 교육 프로그램을 제공하는 좋은 기회였음이 확인되었으나, 일부의 팀에서만 참신하고 재미있는 아이디어들이 제안되었다. 여학생의 참가자 수가 상당히 증가하였으며, 대체적으로 입상권의 학생들의 상당 부분이 여학생이라는 것이 특이할만하다. 학년이 올라감에 따라서 창의적인 아이디어를 내는 양이나 질에 있어서 뒤떨어지는 것으로 분석되었으며, 토론 학습의 적응력이 미흡하고 발표력 및 청취력에 있어서도 떨어지는 것으로 나타났다. 따라서 중 ㆍ고등학교의 학교교육에서도 학생들에게 프로젝트형 탐구학습 프로그램의 적용은 물론 토론식 수습을 통한 학생들의 사고의 전환의 기회를 제공해야 할 것으로 사료된다. The creativity competition was held to foster the creativity of elementary and secondary school students in the Institute of Science Education, Kongju national University. It was found that the creativity competition effected on the improvement of inventing creative ideas and problem solving activities. But, only some competitors participated in semifinal and final contests, presented original and interesting ideas. More female students participated in competition than last year. More female participants were awarded a prize than males. Totally, participants were defective in discussion and communication, and presentation and listening ideas. Increasing grades correlated inversely with creativity and originality. There is need of inquiry project teaming programs and presenting opportunities of conversion of thinking by discussing instructions in school

한국인에서 혈소판 당단백 Ⅲa 유전자 다형성과 급성 관동맥 증후군의 관계

김광일,채인호,김효수,손대원,오병희,이명묵,박영배,최윤식,이영우 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.5

이동 애드 혹 네트워크에서의 환경적응형 QoS AODV 라우팅 프로토콜에 관한 연구

裵振勝,宋東勳,吳世德,鄭燦赫,金玄郁,李光培,文泰洙,郭承郁,河載承 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-

In this paper, we propose a bidirectional mobile Ad Hoc routing protocol based on AODV(ad-hoc on-demand distance vector routing) with QoS(quality of service) support. At presence, wireless mobile communication focuses on how to efficiently support mobility of users more than QoS guarantee. However, in order to satisfy requirement of various applications which have been or will be served, QoS guarantee between end points is a very important issue. The existing AODV routing protocol is implemented to use only BE (Best Effort) service. However, actual wireless mobile environment requires to reduce a waste of communication resources and meet with the real-time change of data paths by setting up routes with QoS support, considering available communication resources at each node in advance. Therefore, in this thesis, we propose an adaptive QoS support method using hello message in order to rapidly meet with the change of available communication resources and to smoothly perform route maintenance. For performance evaluation, we analyzed the average data reception rate. At that time, we used evaluation parameters such as node's mobility-stop time, hello message period, and packet priority, considering the node's mobility and the number of best effort/QoS data flows. As results, we found that with our proposed method the average data reception rate was increased 16.293%, 15.914%, 17.238% for 300 mobility-stop second case and 4.809%, 4.264%, 2.956% for 300 mobility-stop second case.

중합 광원과 중합 시간이 복합레진의 표면 경도에 미치는 영향

배상만,김대업,이광희,안호영 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.2

플라즈마 아크 광원을 사용하는 광중합기를 저출력 할로겐 광원을 사용하는 전통적인 광중합기와 비교 평가하기 위하여 세 종류의 복합레진을 두께가 2, 3, 4, 5mm인 몰드에 충전하고 레진 상면을 할로겐 광으로 40초간 플라즈마광으로 3, 6, 9초간 조사한 후 레진 상면과 하면의 표면미세경도를 각각 측정하였다. 레진시편 상면의 표면경도와 하면의 표면경도 간의 차이는, 두께 2mm 시편에 할로겐 광을 40초간 조사하였거나 플라즈마광을 9초간 조사한 경우들을 제외하고, 모두 유의하였다(P<0.05). 레진시편 상면의 표면경도는 전체 실험군들에서 서로 유의한 차이가 없었다. 레진시편 하면의 표면경도는 전체적으로 보아 할로겐 광을 40초간 조사한 군들에서 가장 높았고 플라즈마광의 조사시간이 감소함에 따라 감소하였으며 레진시편의 두께가 증가함에 따라 감소하였다. 이상의 결과는 복합레진의 중합깊이 측면에서 볼 때 3, 6, 9초간 조사하는 고출력 플라즈마광의 중합능력이 40초간 조사하는 저출력 할로겐광의 중합능력에 미치지 못함을 시사한다. The purpose of study was to compare the plasma are light with the halogen light in compostie resin curing. Three composite resin materials(Z-100. 3M, USA; Tetric Ceram. Vivadent, Liechtenstein; SureFil, Dentsply, USA) were filled in the teflon molds (4mm in diameter and 2,3,4,5mm in thickness) and cured with esther the conventional low-intensity light curing unit with a halogen lamp (Optilux 360, Demetron, U.S.A) for duration of 40 seconds or with the high-intensity light curing unit with a plasma are lamp (Flipo, Lokki, France) for duration of 3, 6, and 9 seconds. The intensity of halogen light was about 370mW/cm2 and that of plasma light was about 1,900mW/cm2. After one week, the surface hardnesses of both the top and the bottom of the resin samples were measured with a microhardness tester(MXT70, Matsuzawa, Japan). There ware significant differences in the hardness between the top and the bottom of the resin samples except the 2mm thickness samples cured by halogen light for 40s or by plasma light for 9s. There was no significant difference between the hardness values of the top surfaces of the thickness groups. The hardness values of the bottom surfaces decreased as the curing time decreased and as the thickness of resin samples increased, and the three kinds of resin composites showed similar patters. The results suggest that the halogen light for 40 seconds might be able to cure greater depth of resin composites than the plasma light far 3, 6, or 9 seconds.

플라즈마 질화처리된 Ti-6A1-4V 합금의 고온 산화 거동에 관한 연구

김광배,배현근,이종수,김영인 한국 항공대학교 항공산업기술연구소 1998 航空宇宙産業技術硏究所 硏究誌 Vol.8 No.-

Ti-6Al-4V 합금에 d. c. 플라스마 질화처리를 실시하고 600℃, 650℃, 700℃의 온도에서 200시간 등온 산화 시험을 실시하여 플라스마 질화처리를 실시하지 않은 Ti-6Al-4V 합금과의 고온 산화 거동을 비교 연구 하였다. 산화 거동은 산화후 시편의 중량 변화량을 측정하여 분석하였으며, 형성층의 미세조직을 관찰 및 상분석을 위하여 주사 전자 현미경 및 XRD를 이용하였다. Ti-6Al-4V합금은 산화 시험 온도 및 산화 시간 증가에 따라 산화 시험후 무게가 증가함을 보였다. 플라스마 질화처리된 Ti-6Al-4V함급의 경우에도 산화 온도 및 산화 시간 증가에 따라 산화 시험후 무게가 증가함을 보였다. 플라스마 질화에 의해 합금표면에 TiN 및 Ti2N의 질화층을 형성시켰으나 산화 시험 온도 범위에서 Ti02층의 형성을 근본적으로 억제할 수는 없었다. 그러나 플라스마 질화처리된 시편의 경우, 플라스마 질화처리된 시편에 비해서 산화 후 무게 증가량이 현저하게 감소하였다. 이는 질화층위에 산화층이 형성되기는 하지만 질화층이 산소의 내부 확산을 저지하는 장벽으로 작용하여 형성 산화층의 성장이 억제된 것에 기인하는 것으로 판단되었다. Plasma nitriding was performed at Ti-6Al-4V alloy. Isothermal oxidation test was carried out at 600℃, 650℃, 700℃ for 200 hours. Oxidation behavior was studied by measuring weight change of specimen after oxidation test. Microstructure of nitrided and oxidized layer was characterized by SEM, XRD, EPMA analysis. Weight gain rate transition of Ti-6Al-4V alloy is parabolic to linear plasma nitrided layer of Ti-6Al-4V was dissociated proportional to oxidation temperature and time. TiN and Ti₂N layer formed by plasma nitriding didn't supress TiO₂formation on the nitride surface. In the case of plasma nitrided Ti-6Al-4V alloy, weight gain rate was sustained at initial oxidation period but decomposed at high temp. Nitrded layer was thermally unstable.