심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

서울지역 여대생들의 떡에 대한 인식 및 섭취 실태 조사

김영순,오순덕,김향미,신지홍,김현경,배유미,조규영,조성환 高麗大學校 倂設 保健大學 保健科學硏究所 2003 保健科學論集 Vol.29 No.1

This study conducted a survey of the improvement and popularization for Korea Rice Cakes, a Korean traditional food, on two hundred women students in five colleges in Seoul, Korea. It was investigated to get the information of the recognition, preference, and consumption patterns for the succession and improvement of Korean Rice Cakes. The results are as follows: 1. A portion of 41.5% among responders knows from three to six kinds of Korean Rice Cakes, and of 28% can make at least one among the kinds of Korean Rice Cakes. 2. A portion of 91% recognizes that Korean Rice Cakes are one of well-nourished food, but of 42% answers that Korean Rice Cakes cannot be replaced with the principle food. Also, there are no significant differences in the recognition of Korean Rice Cakes as the kinds of religions. 3. The frequency of purchase is about one or twice in a month (35.5%). Consumers (36.5%) intend to purchase Korean Rice Cakes for the commemoration of national holidays or anniversaries. 4. The reasons for the preference of other foods more than Korean Rice Cakes are the easiness of purchase (36.0%), the deliciousness (21.5%), the diverse kindness (17.5%), and the proper package (9.5%). It is important that constant and particular school / home education is need to improve and success Korean Rice Cakes.

항암화학요법 관련 오심과 구토 완화를 위한 비약물요법에 관한 연구동향

소향숙,최자윤,조인숙,김영재,김지영,김애숙,김옥미,김춘심,김현오,설영애,안정옥,이애리,이영자 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: Purposes of this study were to understand the current trends on complementary therapy in relieving chemotherapy-induced nausea and vomiting and to suggest the future research direction. Method: Subjects were selected on CINAHL, MEDLINE, Korean Academy Data Base from 1980 to 2001 which used nausea, vomiting, chemotherapy and complementary therapy as key words in experimental studies. Eight korean articles and twenty-one international articles were analyzed in terms of general characteristics, research methods, and types of complementary therapy. Data were analyzed by using descriptive statistical methods. Result: Since 2000, researchers have more actively used complementary therapy. In subject characteristics, mean age was 35.5 years old, 45% of the researchers were performed with high level of incidence of chemotherapy induced vomiting, 14% of them set limit of consecutive cycle during research, and 65% of them did not comment the selecting criteria of sample. About 60% of them were designed post-test only control group: 35% used INV by Rhodes, 31% used Likert scale, and 24% used VAS for dependent variable. Muscle relaxation therapy was mostly applied for relief of nausea and vomiting. Conclusion: Further studies will be needed to control extrinsic variables affecting nausea and vomiting in research design and to accumulate evidence with studies applying various complementary therapies.

포로 체험의 재구성과 기억의 공유

김미향 ( Kim Mi Hyang ) 아시아문화학술원 2017 인문사회 21 Vol.8 No.4

이 연구는 공적 기억인 역사 속에 숨어 있는 개인의 체험과 기억을, 문학을 통해 해석해 냄으로써 한국전쟁 포로 소설의 특징과 존재 방식을 재구해 내고자 했다. 이를 위해 Ⅱ장에서는 전쟁의 목적조차 잃은 채 과열된 갈등으로 폭력과 살육으로 폭주하는 포로들의 모습을 통해 이데올로기의 허구성을 증명하였고, Ⅲ장에서는 전쟁의 직접성에서 벗어나 일상이 전경화 된 상태에서 포로 체험의 기억을 타자와 공유하면서 공감을 불러일으키는 과정을 확인하였다. 포로들의 삶이 개인의 특별한 체험에 불과하다고 치부하거나 또는 `포로`라는 이름으로 이들의 삶을 일률적으로 규정짓지 않고 포로들 각각의 삶을 통해 그 함축된 의미를 고찰해낸다면 이데올로기 대결의 가장 중심에 놓인 핵심적 인물인 포로들이 전쟁으로 고통 받고 희생된 대표적 `비극적 삶의 주체`임을 증명하게 될 것이다. 그리고 이러한 과정을 통해 역사를 보는 시각은 더 다양해지고 우리 전쟁문학의 스펙트럼은 더욱 넓어질 것이다. In the situation tied to the immediacy of war, reorganization of repetitive captive experiences led to tragic ending by making captives` scars fixed. Also, fierce conflicts among captives in the prison camp resulted in cruel slaughter and violence, and Southern Government which had left them in the danger of murder and violence used them politically under the pretense of the release, never considering captives. Captives forgot the purpose of the war and rushed to the violence and slaughter without telling friends from enemies due to excessive conflicts among them, which obviously reveals unrealistic ideology. On the other hand, starting to share the memory of the captive experiences in a state that daily lives are in the foreground, the characters in Kim`s work who are free themselves from the immediacy of war arouse others` sympathy. However, whether the ending is tragic or reconciliatory, it has in common the fact that they are not courageous soldiers or vicious enemies who engaged in the battle field but are normal people and captives of Korean War. They show us that the violence in the prison camp wasn`t individual choice but inevitable result of the war, along with the tragic ending they chose. This makes us check that the captives, key characters placed in the center of ideology confrontation, may be actually representative victims by the ideology, all of whom were most irrelevant to the ideology.

임신에 대한 간호계와 비간호계 여대생의 의식조사 연구

김미향 순천청암대학 1988 論文集-順川看護專門大學 Vol.12 No.-

During 1st to 15th June, 1987, I have collected data from 158 persons university's girl in Kwang Joo in order to grasp their knowledge about pregnancy. From the data, I have gained the following results: The distribution of the respondent' general education 98 persons and the professional nursing 60 persons. 1. General Characteristics 65.2 percent, the higest of the respondents wanted to marry by means of a love marriage being arrarged by a go-between. 26-30 day type menstruation cycle was 53.8 percent and menarche's mean was 15.2 years old. 2. Knowledge about pregnancy The results that show significant difference are summarized as follows: 83.7 percent of general education and 98.3% of proffssional nursing answered to the question of sex determination factor by male sex chromosome. This showed a significant difference between both group.(x^2=8.43, df=2, p<.05) There was just recognized is general education 48.0 percent and proffssional nursing 81.7% in the expected date of confinement accounts. (E.D.C : Last menstruation month-3 and menstruation first of beginning day+7) This showed a significant difference between both group. (x^2=20.08, df=3, p<.001) High pregnancy frequency give bad effect on mother's health. We found a significant difference between both group. (x^2=9.03. df=3, p<.05) To the question of how they came to know the methods of pregnancy knowledge, general education(62.2%) and professional nursing(83.3%) answered "from a technical books". This showed a considerable difference between both group. (x^2=14.17, df=5, p<.05)

Collagen 성숙가교 생성에 관한 기초연구

김미향 신라대학교 자연과학연구소 1999 自然科學論文集 Vol.7 No.-

Collagen, the major constituent of connective tissue fibriles, is the most common protein in the animal world. It is widely distributed in the various tissues of vertebrates and responsible for their distinctive physiological functions. Normal tensile strength in collagen fibrils is due to intermolecular and intramolecular cross-links, which are known to alter in aging. The initial step in the formation of collagen cross-links is the deamination of specific lysyl and hydroxylysyl residues by lysyl oxidase, yielding reactive aldehydes, allysine and hydroxyallysine. Pyridinoline, a mature cross-link which is stable and non-reducible, is derived from two hydroxyallysine and one hydroxylysine residues of collagen fibrils. L-Ascorbic acid(AsA), commonly known as vitamin C, is an essential vitamin for humans, primates, and guinea pigs. AsA had important biochemical functions with various hydroxylations. In the synthesis of collagens, the enzymatic hydroxylations of proline and lysine are necessary for the production of a stable extracellular matrix and cross-links in the fibrils, respectively. To clarify the specificity of AsA in in vitro aging containing enzymatic reaction, lysyl oxidase was partially purified from the aorta of bovine. When AsA were added to the reaction mixture, pyridinoline contents were lower than the control. Moreover, it was observed that AsA is rapidly oxidized within 24 hr under this experimental condition. Supplementation with AsA may decreased pyridionline formation in the early stage of enzymatic reaction in vitro.

일부 도시지역을 유배우여성들의 가족계획 실천정도에 관한 조사연구

김미향 순천청암대학 1989 論文集-順川看護專門大學 Vol.13 No.-

This survey aimed to grasp the actual practice of the family planning of married women in Urban areas. It was carried out through 400 numbers of random selected married women living in Jeonju City from July. 12, 1989 to August. 8, 1989. Questionnaire were used as the research means and the results were used as the research means and the results were analized by percentage and χ^2-test. The summarized results of this survey are as follows; 1. The average age at marriage of the group was 25.67years old, the average frequency of gravidity 4.16, and the number of their living chilgren 2.208. 2. The expieriences of Induced abortion were the average frequency of 1.157.(Age related to the Induced abortion p<.001^(***) level of significant difference) 3. 47.8% unwanted babies by reason of Induced abortion. 4. 68.5% of the group had the actual practice of contraception, and age, education level, religion and Occupation were not related to the significant difference) The most frequently used Contraceptive method was the Tubal Ligation 31.0%, Vasectomy 16.1%, Condoms 13.5%, Menstrual cycle method 13.5% and Oral Pill 6.6% were the next. 5. Regarding to the reasons for not using contraception "because of wanting to have more babies" commanded top ratio 42.1% and "worring about side reaction 19.O% were the next. 6. Most important route from which they knew about the Contraception was "Public health center. Hospital" commanded top ratio 43.3%. 7. 66.0% of the group knew about the correct meaning of family planning.

미국과 일본 미술교육과정의 동향 및 내용에 관한 비교연구

김향미 서울교육대학교 초등교육연구소 1997 한국초등교육 Vol.9 No.2

The purpose of this study is to obtain fundamental materials on the curricular reform measures of art education by comparatively examining the tendency of educational curriculums between the USA and Japan, especially based on the historical curricular changes of elementary art education. The subject that is conclusively suggested is to establish a curricular direction in order to set its general thought and concretely achieve its goal. Through its accumulated achievements of the goal following the establishment of the curricular direction, more concrete theory would be provided and its achievements could be newly expected. A curriculum, a phenomenon situated in a border side between an ideal educational policy and actual educational state, needs to more flexible educational structure, not only by a planned theory as far as it's for human beings having a self-contradiction.

자궁경부 상피내 병변 환자에서 LEETZ 원추절제술 후 HPV DNA Test 추적 검사의 임상적 의의

김미향,이태화,오영림,이천준,김원규 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Objective: This study was performed to investigate the clinical significance and timing of HPV test for follow up of patients after LEETZ treatment in CIN lesion Methods: From January 2005 to December 2008, 105 patients with CIN were treated with LLETZ at Kosin university gospel hospital, Busan, Korea. After LEETZ, HPV test and Pap smear were done after 3months. Every HPV typing was done by HPV DNA chip. Results: A total of 105 cases were enrolled in this study. There were 25 patients with CINⅠ, 38 with CINⅡ, 42 with CINⅢ. Mean age was 41.95 years (range, 23-71 years) at the time of LEETZ. Positive HPV DNA was found about 72.4% before LEETZ procedure and subtype 16 was the most common type (10.5%), follow by subtype 18 (6.6%) and subtype 58 (6.6%). During follow up after LEETZ, 65.6% had negative result after 3 month, 72.1% after 6 month, 73.9% after 9 month, 77.1% after 12 month, 79.2% after 15 month, 79.6% after 18 month, and 80.4% after 24 month. There were 2 cases of abnormal pap smear among persistent positive HPV DNA cases and no abnormal pap smear results in negative HPV DNA cases during follow up. Conclusion: Positive HPV DNA was found about 72.4% before LEETZ procedure and subtype 16 was the most common type (10.5%), follow by subtype 18 (6.6%) and subtype 58 (6.6%). We suggest that HPV DNA test is recommended per 6 Months, and close observation is recommended in persistent positive HPV DNA test cases.

토마토의 생육과정에 따른 성분 변화I : Tomatine 분석을 중심으로

김동석,小机信行,한재숙,김미향 한국식생활문화학회 2004 韓國食生活文化學會誌 Vol.19 No.6

This was aim to study the changes of components by different parts and maturity of tomato. We found that orally fed tomatine which induced a significant reduction in cholesterol in hamster in contrast to potato glycoalkaloids, the safety of tomatine was supported by our observation that pickled green tomatoes consumed widely in many countries has a high tomatine content. The tomatoes harvested during the first stage(10 days after flowering) contained tomatine 6333.49 ㎍ per 100 g and dehydrotomatine and α-tomatine in a ratio of about 1:13.8. The tomatine content then decreased by about 85% during stage 2(20 days after flowering) an dehydrotomatine contene dropped to a value near zero during stage 3, 4. It then the tomatine content dropped to a value near zero during the final stage(50 days after flowering). With respect to health benefits at all stages of maturity, unripe tomato contain chlorophyll and α-tomatine should consumed.