생쥐의 심장발생과정에서 세포증식과 세포고사의 역할

김어진,김효수,서정욱,김어진,김효수,서정욱 대한병리학회 1998 Journal of Pathology and Translational Medicine Vol.32 No.12

실험적 급성 심근경색증 후 좌심실 재성형에 관여하는 간질 조직의 변화에 대한 전환효소억제제의 기전에 대한 연구 : 백서의 非痛璧性 심근경색 후 좌심실 재성형 및 Transforming Grawth Factor β-1의 발현 변화와 이에 대한 레닌 안지오텐신계 차단제의 효과 분석 Effects on Transforming Growth Factor - β-1 Expression

연태진,김석연,김효수,김어진,김소영,정은주,서정욱,오병희 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.9

대동맥판막에 발생한 유두상 섬유탄력종 1례

김응중,최재성,이명묵,김어진 동국대학교 의학연구소 2009 東國醫學 Vol.16 No.1

유두상 섬유탄력종은 주로 심장판막에 발생하는 원발성 종양으로 심장종양 중 두 번째로 많은 양성종양이나 전체적으로 그 빈도는 매우 드물다. 78세 여자 환자가 흉부 불편감을 주소로 내원해서 대동맥판막에 위치한 유두상 섬유탄력종이 발견되어 개심술 하에 대동맥판막의 손상 없이 종양을 제거하였기에 문헌고찰과 더불어 이를 보고하는 바이다. Cardiac papillary fibroelastoma is the rare primary cardiac tumor, though it is the second most common benign cardiac tumor. It usually involves the cardiac valve. Papillary fibroelastoma of the aortic valve was found in a 78-year-old woman with chest discomfort and it was excised without valvular injury under cardiopulmonary bypass. Thus we report the present case with relevant review of the literatures.

1995년 서울지역 거주자에서 발생한 Plasmodium Vivax에 의한 Malaria 1예

김영선,박기령,어은경,최진혁,이정수,정화순 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.2

Malaria has a wordwide incidence of more than one thousand to three thousand million clinical cases and results in approximately 200 to 300 million deaths per year. Although Korea was an endemic area of Malaria, it was nearly eradicated from the country. However, indigenous cases of malaria have been reported since 1994. Recently, we experienced a case of Plasmodium vivax infection in a resident of Seoul. A 25-year old young man who had no history of being abroad, blood transfusion nor parenteral use of drug visited our hospital due to fever and chill for 4 days. A peripheral blood smear demonstrated Plasmodium vivax infection. He was treated with hydroxychoroquine and premaquine without relapse.

Goldman's Algorithm을 이용한 비외상성 흉통 환자의 분석

조석진,유진현,김찬웅,어은경,정구영 대한응급의학회 1999 대한응급의학회지 Vol.10 No.4

Back ground : It is important to evaluate the patient presenting with non-traumatic chest pain in emergency department, and it is also difficult to classify appropriately. The purpose of this study is to predict probability of acute myocardial infarction(AMI) and assess the group for low probability of AMI that is target for observation-unit of chest pain in emergency department. Methods : A prospective, clinical study was performed from March 1998 to August 1998 at the department of emergency medicine, Ewha Womans University Mokdong hospital. We classified high-probability group and low-probability group of AMI according to Goldman's algorithm and then compared with the final diagnosis. Results : The study included 218 patients. 84 patients(39%) was the high-probability group and 134 patients(61%) was the low-probability group of AMI. As compared with final diagnosis, AMI was 47 patients(56%) among the high-probability group and 1 patient(0.7%) among the low-probability group. We observed sensitivity of 98%, specificity of 78% in predicting AMI. Conclusion : The classification according to Goldman's algorithm may be useful predictor of AMI and improve triage for emergency department patients with chest pain. It is also helpful for management of observation-unit of chest pain in emergency department.

식품 및 미세증거물 중 함유전분의 종류 판별에 관한 연구

정진일,민승식,어상희,박유신,김재균 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

자궁경부암 선별검진을 위한 PapSure 검사에서 Spiral Brush의 유용성에 관한 연구

송언호,김어진,명나혜,박충학 대한부인종양 콜포스코피학회 2002 Journal of Gynecologic Oncology Vol.13 No.4

2001년 12월 1일부터 2002년 6월 30일까지 만 7개월 간 단국대학교의료원 산부인과 부인종양 Clinic에서 자궁경부암 선별검진을 위한 PapSure 검사를 시행한 환자 중 SpiraBrush Cx_® 검사를 실시한 33예를 대상으로 하여, 기존의 Papanicolaou 세포진 검사의 진단과 SPiraBrush Cx_®)에 의한 Papanicolaou 세포진 검사의 진단 및 최종적인 병리조직학적 진단을 비교, 분석하여 SpiraBrush Cx_®에 의한 세포진 검사가 생검에 의한 조직검사를 대체할만한 유용성이 있는지 그 여부를 조사하기 위하여 본 연구를 시행하여 다음과 같은 결과를 얻었다. 1. 환자의 연령별 분포는 21세에서 70세까지였으며 40-49세 군이 42%로 가장 많은 분포를 보였고, 평균 연령은 45.7세였다. 2. 환자의 평균 임신횟수는 3.6회였으며, 평균 출산횟수는 2.0회였다. 3. 기존의 자궁경부 세포진 검사(Conventional Papanicolaou cytology)상 진단이 음성으로 나온 경우가 19예(57.6%)였고, ASC를 포함하여 양성으로 나온 경우가 14예(42.4%)였다. 자궁경부 형광경진 검사(Speculoscopy)에서는 음성이 13예(39.4%)였고, Suspicious 진단을 포함하여 양성으로 나온 경우가 20예(60.6%)였다. SpiraBrush Cx®에 의한 세포진 검사(SpiraBrush Cx_® Papanicolaou cytology)에서는 진단이 음성인 경우가 8예(24.2%)였고, ASC를 포함하여 양성으로 나온 경우가 25예(75.8%)였다. 4. 병리조직학적 검사의 진단은 음성 12예(52.2%), 저등급 편평상피내병변(Low-grade Squamous Intraepithelial Lesion: 이하 LSIL이라 함) 2예(8.7%), 고등급 편평상피내병변(High-grade Squamous Intraepithelial lesoin: 이하 HSIL이라 함) 3예(13.0%), 편평세포암(Squamous Cell Carcinoma: 이하 SCC라 함) 6예(26.1%)였다. 5. 검사 결과의 양성판정 기준을 세포진 검사의 경우는 ASC 이상으로 하고 조직학적 검사의 경우는 LSIL이상으로 정할 경우, 기존의 Papanicolaou 세포진 검사의 민감도는 63.6%이고 특이도는 66.7%였으며, SpiraBrush Cx_®세포진 검사의 민감도는 90.9%이고 특이도는 33.3%였다. 이상의 결과로 미루어 볼 때 연구 대상의 숫자가 적어서 통계학적인 유의성은 찾을 수 없으나 SpiraBrush Cx_®가 자궁경부암 선별검진에서 생검에 의한 자궁경부의 조직학적 진단을 대체하지는 않는다고 판단된다. 그러나 기존의 Papanicolaou 세포진 검사의 민감도가 63.6%인데 반하여 SpiraBrush Cx_® 세포진 검사의 민감도는 90.9%로 훨씬 더 높았으며 이로 인해 SpiraBrush Cx_® 세포진 검사의 특이도는 33.3%로 훨씬 낮았는데 이는 매우 흥미있는 사실로서 향후 많은 Data로 더욱 연구하여 진위를 구분함이 필요하리라 사료된다. 또한 본 연구에서는 SpiraBrush Cx_® 기구의 강모들이 단단하고 날카로워서 기저막층까지의 조직과 상피층 세포들을 탈락시키면서 많은 출혈을 초래하여 슬라이드 판독에 어려움을 주었고 이는 연구 결과에 지대한 영향을 미쳤으리라고 생각되는 바, 이러한 출혈로 인한 요인을 최대한 배제하기 위하며 향후 SpiraBrush Cx_® 세포를 채취한 후 최신의 자궁경부 세포진 검사법인 ThinPrep Pap Test를 이용하거나 조직표본을 만들기 위한 Cellblock 기법을 사용하여야 한다고 판단된다. Objective : The purpose of this study was to investigate the clinical efficacy of a new stiff bristled spiral shaped brush (SpiraBrush Cx_(??))∼in) PapSure Test for cervical cancer screening, by comparing SpiraBrush Cx_(??) cytologic diagnoses with conventional Papanicolaou cytologic diagnoses and final histologic diagnoses. Methods : This study was performed in Gynecologic Oncology Clinic of Department of Obstetrics and Gynecology at Dankook University Medical Center from December I, 2001 to June 30, 2002. Thirty three cases who underwent PapSure Test (Pap cytology t Speculoscopy) were analyzed. SpiraBrush Cx_(??) Papanicolaou cytologic diagnoses were compared with conventional Papanicolaou cytologic diagnoses and histologic diagnoses as a reference standard. Results : Followings are the results summarized. 1. Age distribution was between 21 and 70. The mean age was 45.7 years. 2. The mean gravidity was 3.6 and the mean parity was 2.0. 3. There were 19 (57.6%) conventional Papanicolaou cytologic diagnoses of Negative, and 14 (42.4%) of Positive including ASC. There were 13 (39.4%) Speculoscopy diagnoses of Negative, and 20 (60.6%) of Positive including Suspicious. There were 8 (24.2%) SpiraBrush Cx_(??) cytologic diagnoses of Negative and 25 (75.8%) of Positive including ASC. 4. There were 12 (52.2%) histologic diagnoses of Negative, 2 (8.7%) of LSIL, 3 (13.0%) of HSIL, and 6 (26.1%) of SCC, according to the criteria of TBS, 2001. 5. For detecting LSIL or worse lesions, conventional Papanicolaou cytologic test yielded sensitivity of 63.6% and specificity of 66.7%. On the other hand, SpiraBrush Cx_(??) cytologic test yielded sensitivity of 90.9% and specificity of 33.3%. Conclusion : SpiraBrush Cx_(??) cytologic diagnosis does not seem to have clinical efficacy replacing the histologic diagnosis by punch biopsy in cervical cancer screening in this study. But further studies using ThinPrep Pap Test or Cellblock techniques with large data will be required and anticipated.

Tonsillar Metastasis of Small Cell Lung Cancer in a Patient With Idiopathic Pulmonary Fibrosis : A Case Report

Kim, Eo Jin,Kim, So Ri,Jin Gang, Su,Park, Seung Yong,Han, Young Min,Lee, Yong Chul Wolters Kluwer Health 2015 Medicine Vol.94 No.7

<P><B>Abstract</B></P><P>Small cell lung cancer (SCLC) metastasizes widely, but palatine tonsil is an extremely unusual site for metastasis. Idiopathic pulmonary fibrosis (IPF) is associated with increased risk of lung cancer. However, the most common histological findings among patients of lung cancer with IPF are known as non-SCLC such as adenocarcinoma and squamous cell carcinoma. In addition, the majority of them are located in IPF-associated fibrotic peripheral lesions.</P><P>A 77-year-old man visited for 1-month persistent cough and dyspnea, with inspiratory dry crackles on both lower lung fields and a large oval mass in his throat. Chest computed tomography revealed 2 masses in the left lower lobe, 1 mass in the right upper lobe, and multiple enlarged mediastinal lymph nodes of the lung accompanying with IPF, which were diagnosed as SCLC pathologically. Very interestingly, the tonsillar mass was also confirmed as the metastatic lesion of SCLC. Chemotherapy for SCLC and medical treatment for IPF were applied. However, in following-up, he expired due to respiratory failure by an acute exacerbation of IPF 3 months after the diagnosis.</P><P>In this current report, we describe, for the first time, a case of tonsillar metastasis of SCLC with IPF detected simultaneously in a 77-year-old man.</P>

Co‐administration of live attenuated <i>Salmonella enterica</i> serovar Typhimurium expressing swine interleukin‐18 and interferon‐α provides enhanced Th1‐biased protective immunity against inactivated vaccine of pseudorabies

Kim, Seon Ju,Bum Kim, Seong,Woo Han, Young,Uyangaa, Erdenebileg,Hyoung Kim, Jin,Young Choi, Jin,Kim, Koanhoi,Kug Eo, Seong Blackwell Publishing Asia 2012 Microbiology and immunology Vol.56 No.8

<P><B>ABSTRACT</B></P><P>The co‐administration of two or more cytokines may generate additive or synergistic effects for controlling infectious diseases. However, the practical use of cytokine combinations for the modulation of immune responses against inactivated vaccine has not been demonstrated in livestock yet, primarily due to protein stability, production, and costs associated with mass administration. In light of the current situation, we evaluated the immunomodulatory functions of the combined administration of swine interleukin‐18 (swIL‐18) and interferon‐α (swIFN‐α) against an inactivated PrV vaccine using attenuated <I>Salmonella enterica</I> serovar Typhimurium as a cytokine delivery system. Co‐administration of <I>S. enterica</I> serovar Typhimurium expressing swIL‐18 and swIFN‐α produced enhanced Th1‐biased humoral and cellular immune responses against the inactivated PrV vaccine, when compared to single administration of <I>S. enterica</I> serovar Typhimurium expressing either swIL‐18 or swIFN‐α. Also, enhanced immune responses in co‐administered piglets occurred rapidly after virulent PrV challenge, and piglets that received co‐administration of <I>S. enterica</I> serovar Typhimurium expressing swIL‐18 and swIFN‐α displayed a greater alleviation of clinical severity following the virulent PrV challenge, as determined by clinical scores and cumulative daily weight gain. Furthermore, this enhancement was confirmed by reduced nasal shedding of PrV following viral challenge. Therefore, these results suggest that oral co‐administration of <I>S. enterica</I> serovar Typhimurium expressing swIL‐18 and swIFN‐α provide enhanced Th1‐biased immunity against inactivated PrV vaccine to alleviate clinical signs caused by PrV challenge.</P>

칼슘통로 차단제 Diltiazem 및 Verapamil이 배양된 골아세포 MC3T3-E1의 기능에 미치는 영향

김진우,최영길,김영설,김광원,양인명,김성운,서광식,어완규 대한내분비학회 1990 Endocrinology and metabolism Vol.5 No.4

To investigate the effect of calcium channel blockers, diltiazem and verapamil on the function of osteoblastic cell, cloned MC3T3-E1 cells were cultured in media containing 2% fetal bovine serum and later in media containing various concentrations of calcium channel blocker. There results are as followings: 1) There was inverse correlation between concentration of diltiazem and DNA synthesis. The DNA synthesis as percent of control was 93.6 2.0% in 2 M, 57.6 6.2% in 25 M, and 54.6 6.1% in 50 M of diltiazem (p$lt;0.005, p$lt;0.25, and p$lt;0.01 respectively). There was also inverse correlation between concentration of verapamil and DNA synthesis. The DNA synthesis was 96.7 0.1% in 2 M, 61.6 2.1% in 25 M, and 40.9 0.9% in 50 M of verapamil (p$lt;0.005 and p$lt;0.01, and p$lt;0.005 respectively). 2) There was inverse correlation between concentration of ditiazem and C.D.P. (collagenasedigestible protein) synthesis. The C.D.P. synthesis as percent of control was 76.7 2.2% in 5 M, and 44.3 16.0% in 50 M of diltiazem (p$lt;0.005 and p$lt;0.05 respectively). There was also inverse correlation between concentration of verapamil and C.D.P. synthesis. The C.D.P. synthesis was 49.7 0.3% in 10 M and 32.6 8.7% in 50 M of verapamil (p$lt;0.005 and p$lt;0.01 respectively). 3) The synthesis of protein including collagen and the ratio of collagen to protein synthesis were decreased. This implies that calcium channel blocker blocks the synthesis of collagen preferably than that of non-collagen protein. These results imply that calcium channel blocker inhibit DNA synthesis, cell proliferation and the synthesis of collagen, the major organic component of bone matrix. And it is therefore suggested that long-term administration of calcium channel blocker may clinicaly lead to osteoporsis ( J Kor Soc Endocrimol 5:294 302, 1990).