뼈에 발생한 악성 림프종 환자의 치료 효과 판정에서 ^(18)F-FDG Positron Emission Tomography의 유용성

김남돈,박연희,기승석,박용진,김형준,류백렬,김흥태,김성은,천기정,최창운,임상무 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.5

원발성골림프종은 매우 드문 질환이기는 하나, 복합항암요법이나 방사선 치료등에 높은 반응율을 보이며, 좋은 예후를 가지는 림프절의 비호지킨 림프종의 일종이다. 그러나, 치료 효과 판정에 있어 골병변의 특이성에 의한 모호한 방사선학적 특징으로 어려움이 있어 왔다. 본 증례는 위와 같은 원발성골림프종 환자에서 PET 스캔을 사용하여 민감하게 완전 반응을 평가할 수 있었던 예로, 앞으로 다른 고식적인 방법과 함께 이 질환의 진단과 치료 평가 있어 유용하게 사용 될 수 있을 것으로 전망할 수 있었다. Purpose: Accurate assessment of the lesion after treatment of patients with bone lymphoma is difficult. In this patient who demonstrated complete remission after chemotherapy, the regions of fluorine-18 fluorodeoxyglucose (^(18)FFDG)PET uptake diminished more rapidly following therapy, indicating a complete response at much earlier stage than did Magnetic Resonance Imaging (MRI) or CT based findings. With the conventional methods, such as MRI and CT. It was difficult to assess whether the residual tumor tissue was viable or not. Decision to complete response is very important in patients with ymphoma to plan the further treatment. We experienced a patient with primary lymphoma of bone who revealed complete response to chemotherapy on ^(18)FFDGPET while CT showed persistent destructive bone lesion. Thus, ^(18)FFDGPET study after therapy may be superior to CT in the evaluation of response to treatment in primary lymphoma of bone.

Protective effect of Korean Red Ginseng against FK506-induced damage in LLC-PK1 cells

Dahae Lee,Ki Sung Kang,Jae Sik Yu,Jung-Yoon Woo,Gwi Seo Hwang,Dae-Woon Eom,Seung-Hoon Baek,Hye Lim Lee,Ki Hyun Kim,Noriko Yamabe 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.3

Background: Compound FK506 is an immunosuppressant agent that is frequently used to prevent rejection of solid organs upon transplant. However, nephrotoxicity due to apoptosis and inflammatory response mediated by FK506 limit its usefulness. In this study, the protective effect of Korean Red Ginseng (KRG) against FK506-induced damage in LLC-PK1 pig kidney epithelial cells was investigated. Methods: LLC-PK1 cells were exposed to FK506 with KRG and cell viability was measured. Western blotting and RT-PCR analyses evaluated protein expression of MAPKs, caspase-3, and KIM-1. TLR-4 gene expression was assessed. Caspase-3 activities were also determined. The number of apoptotic cells was measured using an image-based cytometric assay. Results: The reduction in LLC-PK1 cell viability by 60mM FK506 was recovered by KRG cotreatment in a dose-dependent manner. The phosphorylation of p38, p44/42 MAPKs (ERK), KIM-1, cleaved caspase-3, and TLR-4 mRNA expression was increased markedly in LLC-PK1 cells treated with 60mM FK506. However, with the exception of p-ERK, elevated levels of p-p38, KIM-1, cleaved caspase-3, and TLR-4 mRNA expression were significantly decreased after cotreatment with KRG. Activity level of caspase-3 was also attenuated by KRG cotreatment. Moreover, image-based cytometric assay showed that apoptotic cell death was increased by 60mM FK506 treatment, whereas it was decreased after cotreatment with KRG. Conclusion: Taken together, these results suggest that the molecular mechanism of KRG in the FK506-induced nephrotoxicity may lead to the development of an adjuvant for the inhibition of adverse effect FK506 in the kidney.

Protective effect of Korean Red Ginseng against FK506-induced damage in LLC-PK1 cells

Lee, Dahae,Kang, Ki Sung,Yu, Jae Sik,Woo, Jung-Yoon,Hwang, Gwi Seo,Eom, Dae-Woon,Baek, Seung-Hoon,Lee, Hye Lim,Kim, Ki Hyun,Yamabe, Noriko The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.3

Background: Compound FK506 is an immunosuppressant agent that is frequently used to prevent rejection of solid organs upon transplant. However, nephrotoxicity due to apoptosis and inflammatory response mediated by FK506 limit its usefulness. In this study, the protective effect of Korean Red Ginseng (KRG) against FK506-induced damage in LLC-PK1 pig kidney epithelial cells was investigated. Methods: LLC-PK1 cells were exposed to FK506 with KRG and cell viability was measured. Western blotting and RT-PCR analyses evaluated protein expression of MAPKs, caspase-3, and KIM-1. TLR-4 gene expression was assessed. Caspase-3 activities were also determined. The number of apoptotic cells was measured using an image-based cytometric assay. Results: The reduction in LLC-PK1 cell viability by $60{\mu}M$ FK506 was recovered by KRG cotreatment in a dose-dependent manner. The phosphorylation of p38, p44/42 MAPKs (ERK), KIM-1, cleaved caspase-3, and TLR-4 mRNA expression was increased markedly in LLC-PK1 cells treated with $60{\mu}M$ FK506. However, with the exception of p-ERK, elevated levels of p-p38, KIM-1, cleaved caspase-3, and TLR-4 mRNA expression were significantly decreased after cotreatment with KRG. Activity level of caspase-3 was also attenuated by KRG cotreatment. Moreover, image-based cytometric assay showed that apoptotic cell death was increased by $60{\mu}M$ FK506 treatment, whereas it was decreased after cotreatment with KRG. Conclusion: Taken together, these results suggest that the molecular mechanism of KRG in the FK506-induced nephrotoxicity may lead to the development of an adjuvant for the inhibition of adverse effect FK506 in the kidney.

임상교정 교육용 멀티미디어 데이터베이스 웹서버 구축에 관한 연구

박재우,이종기,장영일,남동석,김명기,양원식,김태우,백승학 대한치과교정학회 2000 대한치과교정학회지 Vol.30 No.2

본 연구는 인터넷상의 가상공간에 다양한 자료로 구성된 환자 증례를 통해, 교정을 배우고자 하는 임상의나 학생들의 교육에 필요한 멀티미디어 데이터베이스를 구축하기 위한 것이다. 이러한 시스템을 구현하기 위해서는 이미지 자료의 처리, computer network를 통한 빠른 정보처리 기능, 진단 분석법의 개발, 데이터베이스의 구축 기술, 데이터베이스와 인터넷과의 연계, 증례를 통한 교육방법론과 같은 기술적 문제점들이 있다. 진단과 치료계획을 위한 분석은 모델 분석과 두부방사선 사진분석으로 나누어 제공하였다. 모델분석법은 arch length discrepancy와 Bolton tooth ratio를 환자 정보란에 제공하였다. 두부방사선 사진분석은 초진란에 제공하였다. Cephalometric analysis ver 2.0과 Power Ceph pro 3.3.5를 이용하여 Tweed, Steiner, Jarabak분석법 등을 제공하였으나, 본문에서는 주로 Kim's analysis와 몇 가지 계측치를 추가하여 설명하였고, 나머지는 tracing란에서 제시하였다. 또한 치료 종료 후나 보정란에서 치료 전, 후의 두부방사선 사진을 중첩해서 보여줌으로써 치료에 의한 효과를 직접 볼 수 있도록 하였다. The Objective of this research was to construct the multimedia database system that was necessary for the education of the practitioners and the students. To establish this system, there were technical problems as follows : 1) The processing of the images, 2) The rapid processing of the information with the computer network, 3) The development of diagnosis tools, 4) The technique to establish the database system, 5) The link between the database system and the internet, and 6) The method to educate through many cases. The analysis for diagnosis and treatment planning were provided as two parts : model analysis and cephalometric analysis. As a model analysis, arch length discrepancy and Bolton tooth ratio were provided for the part of patient information. Cephalometric analyses were provided in the part of initial diagnosis. The Cephalometric analysis ver 2.0 and the PowerCeph pro 3.3.5 were used to show Tweed, Steiner and Jarabak analysis. In the main part, Kim's analysis and some measurements were added. In the post-treatment or retention part, we show the superimposition of the cephalometrics with which you can find the effectiveness of the various orthodontic treatment. The address of this home page is "http://damis.snu.ac.kr/orthodontics"

정상교하 아동의 두개안면부 성장에 관한 종적 연구

양규호,박창헌,손정수,김낙현,최남기,김선미,김기백,신혜성 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.3

혼합치열기 정상교합 아동 24명(남:14명 여:10명 초진 시 평균 나이 9±1.3세, 평균 관찰 기간: 13±1.3개월)에 대한 성장량을 측정하여 기능적 교정장치의 순수 치료효과를 평가하는데 도움이 되기 위해 3회(5~8개월 간격) 촬영한 측모 두부 방사선 규격 사진에 대한 분석 결과 다음과 같은 결론을 얻었다. 1. 남아는 상악골은 전하방, 하악골은 전방성장하였고, 여아는 상하악골이 전하방 성장하였다(p<0.05). 2. 상하악골의 남녀간 차이에서 수평적 성장상태는 여아가 컸고(A point 여아: 2.39mm, 남아: 1.26mm, p<0.05), 수직적 성장상태는 유의한 차이가 없었다. 3. 상악 전치의 치축은 두개저에 대해서 순측 경사하였고(p<0.01) 하악 전치의 치축은 큰 변화가 없었다. The purpose of this study was to provide the reference data evaluating the treatment effect of orthopedic appliances. The skeletal and dental growth increments were measured in 24 normal mixed dentition children(boys: 14, girls: 10) by three serial lateral cephalograms: initial mean age: 9±1.3 years, mean observation period: 13±1.3 months, Cephalometric changes were analysed. The results were as follows: 1. In boys, the maxilla showed forward and downward growth pattern and the mandible showed forward growth pattern(p<0.05). In girls, the maxilla and mandible showed forward and downward growth pattern(p<0.05). 2. Horizontal growth of both maxilla and mandible in girls was superior to those in boys(A point: girls: 2.39mm, boys: 1.26mm, with p<0.05), whereas vertical growth of both maxilla and mandible in boys was similar to those in girls. 3. The change in tooth axis showed labioversion of upper incisor(p<0.01) and comparatively stable lower incisor position.

신증후출혈열 환자의 역학조사를 위한 혈청학적 분석 및 항원검출

백락주,송기준,김상현,윤재경,김세연,송진원,이용주 대한감염학회 1997 감염 Vol.29 No.5

목 적 : 1991년 한탄바이러스 예방백신접종이 본격적으로 시행된 이후의 신증후출혈열 발생양상 및 한탄바이러스와 서울바이러스에 의한 감염비율에 대한 전반적인 역학조사와 새로운 한탄바이러스종의 존재 가능성을 증명하기 위하여 혈청학적 검사와 환자혈청에서의 한탄바이러스 항원검출을 시도하였다. 방 법 : 1994년 1월부터 1996년 12월까지 3년간 발생한 열성질환 환자중 신증후출혈열, 리케치아증 및 렙토스피라증 등이 의심되어 항체검사를 위해 고려대학교 의과대학 미생물학교실, 바이러스병 연구소에 의뢰된 혈청 8102건을 대상으로, 간접형광항체법, 혈구응집저지반응, 면역효소측정법 및 역전사 중합효소연쇄반응을 이용하여 한탄바이러스 감염에 대한 혈청역학적 연구 및 항원검색을 수행하였다. 결 과 : 간접형광항체법으로 조사한 한탄바이러스에 대한 항체 양성율은 30대 남자에서 가장 높았으며 10월에서 1월사이에 항체 양성 건수가 집중되어 있었다. 신증후출혈열 발생 빈도가 감소하고 있었으며 특히 건조기에 집중되었던 발생양상의 변화가 관찰되었다. 한탄바이러스에 의한 감염이 서울바이러스에 의한 감염보다 3배이상 높게 관찰되었으며 서울바이러스에 의한 감염은 고령층에서 더욱 빈발하였다. 한탄 및 서울 바이러스 이외의 새로운 한탄바이러스에 의한 감염 가능성이 확인되었다. 결 론 : 전반적으로 발생빈도가 감소하였으나 현재에도 우리 나라에서는 매년 수백명의 신증후출혈열 환자가 발생하며 한탄바이러스와 서울바이러스가 주요 원인체이나 새로운 한탄바이러스종의 존재 가능성이 확실하여 이의 규명을 위한 지속적인 연구가 수행되어야 할 것으로 사료된다. Background : Hantaan (HTN) and Seoul (SEO) viruses, harbored by the stripedfield mouse(Apodemus agrarius) and the Norway or common rat(Rattus rattus & Rattus norvegicus) respectively, were known to cause hemorrhagis fever with renal syndrome(HFRS) in Korea. We evaluated the seroepidemiologic patterns of hantaviral infection and detect the hantaviral antigens from patients sera. Methods : total 8,102 HFRS patients' sera were collected from 1994 to 1996, and examined by indirect immunofluorescent antibody technique(IFA), hemagglutination inhibition test(HI), IgM emzyme-linked immunosorbent assay(IgM-ELISA) and nested reverse transcription-polymerase chain reaction(RT-PCR). Results : The seropositive rate against hantaviral antigen was 12.0%(973/8102) with the high incidence rate(68.3%) in the period from October to January, and males in the thirties were mostly affected. HTN viral infections were detected 3.5 and 5.2 times higher than SEO viral infections by HI and RT-PCR, respectively, and patients in the fifties were the mostly affected age-group in SEO viral infections. IgM antibodies were detected in the 717 sera of the 905 IFA positive cases(79.2%), and the antigen detection rate of HTN and SEO viruses was 7.7%(56/724). Interestingly, 40 sera(4.4%), showed higher antibody titers against the puumala(PUU) virus than those against HTN or SEP viruses. Conclusion: The results showed HTN and SEO viruses were the main causative agents of HFRS in Korea, and also suggested the possible presence of PUU-related hantaviral infections.

조립식 컨테이너 시설활용 기술개발을 위한 기초연구

백기영,김환식,김영희 永同大學校 2000 硏究論叢 Vol.6 No.1

본 연구는 기존 조적조 주택에 컨테이너를 주요 구조부로 활용한 컨테이너 하우스의 계획, 설계, 시공상 유의점 도출을 목적으로 한다. 바닥구조, 벽구조, 기둥 등에 한하여 주요 구조부재인 수평재의 구조성능 규명과 설계기준 작성을 위한 기초자료를 도출하였다. 조립식 컨테이너의 유형별 활용방안 및 계획, 시공에 관련된 분석을 통해 기업체의 기술개발 및 투자방향을 제공하였다는데 의의가 있다. This paper aims at searching the attentiveness in planning, design and construction procedure of the container house where container is used as core framework. And we try to produce research fundamental materials for the construction performance and design standard. This study is significant in that produces direction of technical development and investment through general analysis in planning, design, and practical use for the prefabricated container house.

지식베이스를 이용한 영역이해에 관한 연구

金栢起,鄭日和,崔寅浩,李大寧 慶熙大學校 1994 論文集 Vol.23 No.-

본 논문은 지식베이스를 이용한 영역이해에 관한 것으로 영역지식원과 제어지식원을 분리, 영역 지식원을 특징량에 따라 계층적으로 모델링하여 시스템을 구현함으로써 화상의 이해율 및 지식원의 정합율을 향상시키고 검증처리속도를 개선하였으며 전문적인 경험적 지식의 집적화와 갱신이 가능하도록 하였다. In this paper, we present a region understanding method using knowledge base. we splited domain knowledge sources and control knowledge sources and implimented image understanding system modeled on domain knowledge sources hiearchically according to minutia. The purposes of this paper are to improve the understanding rate for images and the matching rate of knowledge sources and to advance the processing times for verification. Therefore, it is possible to integrate and renovate the technical heuristic-knowledge.

靈芝버섯 栽培方法 改善에 關한 硏究

金成朝,李甲湘,李基炫,白承和 圓光大學校 1993 論文集 Vol.27 No.2

靈芝載培에 있어서 一般的인 栽培法을 改善하여 靈芝의 收穫量을 높이고 品質向上을 시킬 目的으로 菌絲의 活着條件, 種菌接種 時期 및 方法, 버섯培養地의 土養種類 등을 달리하고, 栽培舍 構造設備을 改良하여 靈芝를 栽培한 結果는 다음과 같다. 種菌接種短木에 菌接種時期가 1月, 12月, 2月, 3月, 4月, 順으로 氣溫이 올라 갈수록 菌絲活着 期間이 빨라져 버섯培養할 때 까지의 菌絲培養 期間을 10日 以上 短縮할 수 있었다. 菌絲培養床의 크기 中 菌絲培養床 幅을 各各 0.8m,1.2m로 하였을 때 같은 菌絲培養期間 동안의 菌絲活着率은 89.6%, 96.4%, 91.2%가 되어 菌絲培養床 幅이 1.0m에서 菌絲活着率이 가장 높았다. 接種短木에 種菌을 接種 後 密封方法은 스치로폴을 막은 後 파라핀으로 密封한 것이 菌絲培養條件에 關係없이 푸른곰팡이 發生率이 가장 낮았고, 스치로폴만 막은것도 新聞紙를 材料로 菌絲培養 條件을 改善하여 푸른곰팡이의 發生率을 파라핀으로 完全히 密封 것과 거의 같은 水準으로 抑制 시킬 수 있었다. 二重 비닐하우스 栽培舍內의 內部 하우스에 遮光幕을 設置함으로서 一般 裁培舍 하우스의 6個月 水準의 靈芝生育 適溫期間을 8個月 以上 까지 延長할 수 있었고, 收穫回數를 年 2回에서 4回로 늘릴 수 있었다. 內部 하우스에 遮光幕을 設置하여 改善한 栽培舍에서는 버섯培養 後 2年次에 目標水準 收穫量의 96.9% 까지 收穫할 수 있었는데 一般 栽培舍에서는 目標 收量의 89.1%만 收穫할 수 있었다. 收穫量에서도 改良 栽培舍의 上品靈芝收量이 一般 載培舍에 比하여 1.5倍의 增收를 보였다. 粘土含量이 가장 많고 모래 含量이 적은 E 土壤에서 雜菌發生이 가장 甚하여 靈芝의 收穫量 및 品質이 가장 低調하였다. To increase the yield and to obtain much better the quality on grown Ganoderma lucidum cultured, these experiments were conducted under carefully controlled in greenhouse with the following conditions on spawn spread related with tentative planting time of short timber inoculated, sealing methods with styrofoam only used and with sealing wax after styrofoam used, soil textures of planting sites, temperatures and growing time as the outside shield was commonly culture method and the inside shield was one of the methods improved itself in these experiments with double vivyl greenhouse. The results were as follow: Speeds of spawn spreading on inoculating Ganoderma lucidum to the short timber were faster when the temperatures were increasing as Jan.<Dec.<Feb.<Mar.<Apr., and it could be short more than 10 days for tentative planting time with inoculation. During the same time of tentative planting the rate of spawn was the highest at the width of 1.0m when the widths of tentative planting hotbets were 0.8, 1.0, and 1.2m, and the rates were 89.6, 96.4, 91.2%, respectively. Occurrences of green mold on inoculated timbers were the lowest as the sealing wax after styrofoam used without the conditions of tentative planting, and when there were surrounding the pile of timbers with styrofoam only used to seal inoculating holes with the second hand newspapers, those were almost the same level as the sealing wax after styrofoam used. When the shield over the roof of the inside greenhouse in the double greenhouse was set, the time on growing could be prolonged until more than 8 months and the yield could be increased 2 times more from 2 to 4 times as compared with the outside shielding over the vinyl that was the common culture method to be known. The culture with inside shielding could improve to be possible that the 96.9% of objective yield was done during two years only and that the amount of the best qualities was 1.5 times than in the common culture, the outside shielding, there was 89.1% of yield only at the same time. In sandy caly that was the least sand and was the most clay of the soils experimented there was the least of yield, there were the plenty of various germs occurred and there was not good in the quality.

Platelet-Derived Growth Factor가 백서두개관 세포군의 증식 및 교원합성에 미치는 영향

김기수,고성희,백정화,민병무,김관식,정동균 대한구강생물학회 1991 International Journal of Oral Biology Vol.15 No.2

To study the effect of platelet-derived growth factor(PDGF) on the replication and collagen synthesis of rat calvarial cells, five bone cell populations(I-V) were prepared from fetal rat calvaria by sequential enzyme digestion. After primary culture for 6-7 days, each bone cell population was collected and then population Ⅰ and Ⅱ, Ⅳ and Ⅴ were pooled together. And the cells were resuspended at 6-8×10^4 cells/㎝^2 and cultured for 2-3 days. The medium was changed to serum-free medium prior to addition of growth factor. The effect of PDGF on the cell proliferation was measured by the incorporation of [^3H]thymidine into DNA. Protein synthesis was determined by measurement of [^3H]proline incorporation into collagenase-digestible protein(CDP) and noncollagenous protein(NCP) according to the method of Peterkofsky and Die-gelmann(1971). The observed results were as follows. 1. PDGF at 10 ng/㎖ significantly increased the [^3H]thymidine incorporation into DNA in all bone cell populations. 2. PDGF at 30 ng/㎖ significantly increased the synthesis of NCP in population Ⅰ, Ⅱ and Ⅳ, Ⅴ. 3. PDGF had no effect on the synthesis of CDP but percent collagen synthesis was decreased significantly in population Ⅳ, Ⅴ. Taken together, the increase of protein synthesis by PDGF in rat calvarial cells was due to the incraese of NCP synthesis.