실험적 진폐증에서 투여약제에 따른 면역학적 병리학적 조직변화

윤임중,임현우,노영만,오상용,정장영,임영,김경아 가톨릭 대학 산업의학 쎈타 산업의학연구소 1993 韓國의 産業醫學 Vol.32 No.2

To access the effects of some drugs such as piperazine phosphare, azathioprine and allopurinol in the experimental pneumoconiosis, 263 rats weighted 240-320 gm was divided into the control and eight experimental groups. To each group, the turbid solution mixed free silica, talc and natural coal dust of 50 mg in 0.8 ml saline were instilled intratracheally. At the begining and 5th week of experiment, piperazine phosphate(8mg/week orally), aspirin(1.5mg/day intramuscularly), azathioprine(25mg/day orally) and allopurinol(1.5mg/day orally) were administered simultaneously, while the dust turbid solution was only given to the control group. For each group the change of body weight. dry right lung weight, cellularity including total cell, macrophage, lymphocyte and neurtrophil in bronchoalveolar lavage fluid, superoxide ion, hydroxyproline, leukotriene B₄, tumor necrotsis factor, prostaglandin E₂were examined, and observed the pathological chages in lung tissue. The results were as follow : 1. The larger amount of their instilled dust and the longer observation period, the severer pathological findings were found in the lung tissue of each experimental group. 2. The body and dry right lung wight was not significantly changed in the experimental groups compared with the control group. 3. The number of total cell in bronchoalveolar lavage fluid was markedly less in the experimental group than the control one, while no significant difference was found in the percent alveolar macrophages and lymphocytes in both groups. However, the percent of neutrophiles was usually lower than in the control group. 4. The amount of superoxide ion was frequently less in the experimental group compared with the control one. 5. The amount of hydroxyproline was significantly lower in the experimental group than in the control one. 6. The amount of leukotriene B₄was usually less in the drug administered group than in the control one. 7. The amount of tumor necrotic factor was also frequently lower in each experimental group than in the control one. 8. The amount of prostaglandin E₂was usually higher in each experimental group than in the control one.

Sprague Dawley 수컷 랏트에서 Bisphenol A Diglycidyl Ether의 경구 투여에 의한 급성 독성

임재언,양윤정,이태진,홍연표 대한산업의학회 2006 대한직업환경의학회지 Vol.18 No.4

목적: 본 연구는 BADGE의 경구 투여에 의한 급성 독성학적 연구를 수행하여 기존의 연구 결과를 확인하고 추후 내분비계 장애 연구에 필요한 기초자료를 얻고자 하였다. 방법: 수컷 SD 랏트에 0, 1000, 2000, 4000과 8000 mg/kg/day의 BADGE와 DES 0.37 mg/kg/day의 농도를 단회 경구로 투여한 후 14일간 일반 증상 등을 관찰하였고 14일 후 부검하였다. 결과: BADGE를 투여한 모든 군에서 투여 후 3일째에 설사와 다른 일반 증상들이 관찰되었고 체중도 감소하는 경향을 보였다. 특히 8000 mg/kg/day의BADGE를 투여한 랏트에서 투여 후 3일째에 대조군에 비해 유의한 수준으로 체중이 감소하는 것을 관찰할 수 있었다. 일부 BADGE 투여군에서 대조군에 비해 심장(1000, 2000과 4000 mg/kg/day), 간(1000, 2000, 4000과 8000 mg/kg/day)과 전립샘(4000 mg/kg/day)의 무게의 감소가 관찰되었다. BADGE 투여군의 간(1000과 4000 mg/kg/day)과 전립샘(8000 mg/kg/day) 의 상대 무게가 대조군과 차이를 보였다. 고환을 제외한 모든 장기에서 조직학적인 변화는 관찰되지 않았으며 고환에서는 세 정관에서 정자세포의 감소가 관찰되었다. 고환과 부고환의 sperm head 수를 보면, 고환에서만 감소하는 경향을 보였으며 정자의 운동성과 기형은 대조군과 차이가 없었다. 혈장 에스트로겐과 테스토스테론 농도는 대조군과 모든 투여군 간의 유의한 차이가 없었다. 결론: BADGE를 랏트에 경구 투여 하였을 때 1000 mg/kg/day의 수준에서도 일반 독성 및 생식독성을 유발시킬 수 있음을 보여준다. Objectives: Bisphenol A Di Glycidyl Ether (BADGE) is the major component in commercial liquid epoxy resins, which are manufactured by co-reacting bisphenol A with epichlorohydrin. The authors investigated the acute toxicity of BADGE. Methods: BADGE was administered by a gavage to 8 week old SPF Sprague Dawley rats in a single dose of 0 (negative control), 0.37 (Diethylstilbesterol, DES), 1000, 2000, 4000, and 8000 mg/kg/day of BADGE. Each treatment group contained 7 rats. The general status and weight of the rats were observed for 14 days. The rats were anesthetized by ether at 14 days, and the changes in morphology, organ weight, sperm count and motility, and hormone level were measured. Results: All the rats treated with BADGE had diarrhea on the 1st day. The rats administered BADGE at 1000, 2000, and 4000 mg/kg/day showed a soiled perineal region and soft stools with diarrhea until the 3rd day. The 8000 mg/kg/day BADGE rats had diarrhea for two days followed by emaciation, soiled fur, a soiled perineal region, staining around the mouth and were moribund for three to eight days. No weight gain was observed after the 1st day in the 2000, 4000, and 8000 mg/kg/day BADGE rats and after the 7th day in all the treatment groups compared with the control groups. Some treatment groups were observed to have a decrease in the weight of the heart (BADGE 1000, 2000 and 4000 mg/kg/day), liver (BADGE 1000, 2000, 4000 and 8000 mg/kg/day) and prostate (BADGE 4000 mg/kg/day) compared with control group. The weight of the liver was significantly lower in all treatment groups compared with the control group. The relative weight of the liver (BADGE 1000 and 4000 mg/kg/day) was significant lower than the control. No pathological changes were observed in the brain, liver, thyroid, heart, spleen, kidney, lung and prostate. The number of spermatid in the seminiferous tubule in the testes was lower in all treatment groups than the control. The sperm motility tended to decrease with increasing concentration but the sperm count was similar in all treatment groups. The plasma Estrogen and testosterone level were similar in the control and treatment groups. Conclusions: These results suggest that BADGE induces general, hepatic and reproductive toxicity at 1000 mg/kg/day.

응급구조사 수습 과정에 대한 고찰

정경임,황정연 대한응급의학회 1993 대한응급의학회지 Vol.4 No.2

We performed this study to evaluate the efficacy of Emergency Medical Technician Education in a designated Facility. This article is the statistical analysis of which the results were obtained from the first trial of Emergency Medical Technician Training program by EMS instructor at Emergency Department, Wallace Memorial Baptist hospital in Pusan from Jun 28 to July 10, 1993. The results were as follow : 1) On sexual distribution of students, male was 57.6% and female was 42.4%. On age distribution, between 30-39 year-old age was 64.7% average age was 33.5 year-old. 2) The geographic distribution of their address was 57.6% in Pusan, the remainders in Kyung-nam, Kyung-buk, and Che-ju in order. 3) The average bed size of the hospital in which they served was 375 beds. 4) The students working in Emergency Room was 42.4%, the other was 57.6%. 5) There were 53.3% students who have the license for nurse-aid and 46.7% registered nurses. 6) The period of students clinical experience was for 101.8 months (about 8 years) in average, and their career in emergency room was for 38.1 months(about 3 years). 7) In the lecture, 91.1% of the students answered that they were content with the lecture qualities or that it was sufficient to learn. In learning the skills, 73.5% of the students replied they were content with the quality to teach the skills or it was sufficient one. Especially male students as a nurse-aid had the better results for the acquisition of the skills than the female registred nurse. 8) 57.6% answered their lecture time was short, 42.1% was proper. Most students were satisfied with the time length of their curriculum, but 26.5% of the students wanted to have more than 12 weeks of education couse. Most of them replied the proper size of the class must be below 20 students per class. 9) They answered the lectural time per a learning item was plenty in 72.0% of the students, not in 28.0%, and skill acquisition time per a learning item was appropriate for 41.4% of the participants, not for 58.6%. They answered the education materials were plenty in 85.3% and not in 11.8%. 10) The written tests showed normal distribution curve through the 4th written test and the results of the final written examination was 98.5 points in average. 11) There was very high correlation(co-efficient : 0.6234) between the 3rd and 4th written test and relatively high correlation between the 3rd and final test and between 4th written test and the skill tests. 12) The female students got the higher points than the male in the written test, but no significant differences of the points in the skill testing according to the participants sex. 13) The registered nurses made the better points in the written test compared to the nurse-aids, but statistically there was no significant difference in the skill testing. To develop better education program for EMT the counterplans on the basis of above results can be established as belows. 1) It will be necessary to ensure professional people who aquired the sufficient training and experience for emergency treatment and to look for legal basis. 2) The sufficient training for EMT is wanted and both starting education and continuing education needs to be focused. 3) The education program for EMT has to be varied according to its charateristics of qualification. 4) the special education program for lecturer for EMT is needed. 5) Each Medical and Education organization should work on the entire education course achieve its standardization of time length, content, methods and level. 6) The positive attitude of government is needed to solve the problems which happen with the current system of Emergency Medicine. It will be necessary to stipulate the items relevant to Emergency Medicine in the law.

신부전이 동반된 당뇨병 환자에서 발생된 침습성 모균증 : 수술과 Liposomal amphotericin B 및 GM-CSF 병합 요법에 의한 성공적인 치험 1예

이원영,오기원,임국희,장재혁,이동건,최정현,강무일,신완식,차봉연,이광우,손호영,강성구 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.4

저자들은 신부전이 동반된 당뇨병 환자에서 발생된 부비동형 모균증에 대하여 수술과 함께 liposomal amphotericin B 및 GM-CSF의 복합치료를 하여 성공적으로 치료한 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Mucormycosis (zygomycosis) primarily affects diabetic or immunocompromised patients and typically progresses rapidly, necessitating surgical excision and antifungal therapy with amphotericin B. Large doses of amphotericin B are needed for cure, but it has the risk of causing significant renal toxicity. The recent development of liposomal amphotericin B allows antifungal therapy to be administered with potentially improved efficacy and reduced nephrotoxicity. We have experienced a case of paranasal mucormycosis successfully treated with surgery, liposomal amphotericin B and GM-CSF. A 59-year-old male suffering from diabetes mellitus for 6 years was admitted with pain at left maxillary area. He was diagnosed as mucormycosis after cytologic exam on the necrotic nasal mucosa, which showed typical hyphae. He have had diabetic nephropathy with macroproteinuria and had rapidly rising serum creatinine levels with the amphotericin B treatment: creatinine levels reverted to basal level with the use of liposomal amphotericin B. Despite surgical excision and continued antifungal therapy, his infection was not effectively controlled. Therefore, GM-CSF was administered additionally to improve phagocytic activity of leukocytes. He was finally cured after receiving a combination of aggressive surgery, liposomal amphotericin B and GM-CSF. To our knowledge, this is the first detailed clinical description of the treatment of mucormycosis with liposomal amphotericin B in Korea.

학교 주5일제에 관한 학생과 교사의 의식 분석

홍광식,최영자,이연화,정정임,장남덕 한국초등교육학회 2002 초등교육연구 Vol.15 No.1

본 연구는 학교 주5일제에 관한 학생과 교사의 의식을 조사분석하여 학교 주5일제 실시에 대응하고자 하였다. 조사 대상자는 초·중·고 학생 1558명과 교사 1077명이었다. 연구결과, 학생들은 현재 학교생활에서 공부하는 즐거움을 알지 못하고 친구들과의 교제에서 즐거움을 찾고 있는 것으로 나타났다. 학교 주5일제 관해서는 학생과 교사 대부분이 찬성하고 있으며, 찬성 이유는 여유있는 하루, 가족과 접촉시간을 갖고 취미활동을 하며 친구와 놀 수 있기 때문이라고 하였다. 교사들은 학교 주5일제로 인한 수업시수의 문제를 감축해도 상관이 없다는 반응이 많았고, 현재의 수업시수를 확보해야 한다는 의견은 적었다. 학생과 교사 대부분은 학교 주5일제는 학생들이 다양한 체험을 할 수 있는 기회를 제공하는 체제로 인식, 긍정적인 반응을 보였다. The purpose of this study is twofold: (ⅰ) to analyze students and teachers' consciousness on the proposed Five-day-work-week School System(FSS) plan; and (ⅱ) to propose a desirable direction of school management including curriculum compatible with the (FSS) plan. This study developed as follows. For the theoretical background of this study, previous research was reviewed. For the items of the questionnaire, the Japanese Ministry of Education Survey on the FSS at school was referred to. The Subjects sampled were 1558 students and 1077 teachers, The data collected from the at elementary, secondary and high school of students and teachers were statistically analyzed by using the SPSS WIN 7.5. The following are the results of the survey. First, in order to adopt the FSS plan, schools have to develop new programs connecting home and school. It is required that school develop special programs that the local community can participate in and cooperate with. Second, most of the respondents support the FSS plan because students can have more chances to be with their family, to become familiar with nature, and to attend various activities sponsored by the local organizations. Third, to argue that it is desirable to adopt the FSS plan even though school has to reduce the minimum class hours. The reason they are against the no-school-on-saturday plan is that there are many families who cannot take care of their children on Saturdays; thus, special programs for them should be prepared before the plan is put into practice. It is also required that we develop various sports or culture events for young adolescents and make up more organizations for youth. We should have school or community facilities open to young adolescents and children whose parents are unavailable to care for them. The latter should have an easy access to those facilities. From these observations, we can conclude that it is imminent to develop various programs before we put the FSS plan into practice. Schools should cooperate fully with homes and the community.

랫드에서 미생탕의 4주 반복투여독성

김태명, 류재면, 서임권, 박동선, 조정희, 장용훈, 김윤배, 황석연 충북대학교 동물의학연구소 2009 Journal of Biomedical and Translational Research Vol.10 No.1

본문참고

제왕절개술의 전신마취 유도중 전완고립술과 Bispectral Indes를 이용한 마취깊이의 평가

김계민,이윤석,연준흠,임윤희,홍기혁,최원주,김정원 대한정맥마취학회 2000 정맥마취 Vol.4 No.3

Case Report : Rupture of abdominal aortic aneurysm after spine surgery in the patient with Ehlers-Danlos syndrome

( Jung Sik Im ),( Yun Hee Lim ),( Jung Sun Park ),( Sang Seok Lee ),( Kye Min Kim ) 대한마취과학회 2010 Korean Journal of Anesthesiology Vol.58 No.6

Ehlers-Danlos syndrome (EDS) is a rare inherited disorder of the connective tissue that is characterized by hyper-extensible skin, hypermobile joints and abnormalities of the cardiovascular system. A 15-year-old girl with Ehlers-Danlos syndrome underwent thoracolumbar surgery for deformity correction. After surgery, an abdominal aortic rupture occurred, and she complained of abdominal distension had an abdominal circumference of 80 cm. Abdominal computed tomography revealed a pseudoaneurysm and a large hematoma at the retroperitoneum. She died of a massive hemorrhage during subsequent abdominal aortic surgery. (Korean J Anesthesiol 2010;58:555-559)

Effects of Extracellular Stimulation of Different Niche Condition on the Transcriptional Regulation of Matrix Metalloproteinase Genes in the Mouse Embryonic Stem Cells

Jung Im Yun,Min Seong Kim,Seung Tae Lee 한국동물번식학회 2013 Reproductive & developmental biology Vol.37 No.2

Classification of Lily Cultivars and Lines by Genotyping bySequencing (GBS) and Phenotyping

Yun-Im Kang,Young Ran Lee,Youn Jung Choi,Kang-Joon Choi,Chan Gu Lee,Yoon Jung Hwang,Ki-Byung Lim 한국원예학회 2017 한국원예학회 학술발표요지 Vol.2017 No.10