Effect of Non-animal-Derived Nitrogen Sources on the Production of Hyaluronic Acid by Streptococcus sp. KL0188

Lee, Gil-Yong,Ha, Suk-Jin,Jung, Jong-Hyun,Seo, Dong-Ho,Park, Jong-Yul,Kim, Su-Rin,Park, Nam-Woo,Kweon, Dong-Keon,Park, Sang-Hoo,Park, Cheon-Seok The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.3

Hyaluronic acid (HA) is a linear high-molecular-weight polysaccharide with useful biomedical applications. Streptococcus zooepidemicus, a typical HA-producing bacterium, requires an animal-derived nitrogen source such as tryptone, peptone or sheep blood as a nutrient. Sixteen non-animal-derived (NAD) nitrogen sources were tested as a replacement for the expensive animal-derived nitrogen sources, which may have safety issues. Among the sixteen tested NAD nitrogen sources, a yeast-derived nitrogen source (YE 0251) showed the highest HA productivity, which was equivalent to the control HA production medium containing tryptone in a 5-L jar and in 3,000-L industrial fermentations. In the 3,000-L fermentation, YE 0251 increased cell mass (dry cell weight) and HA production by 11% and 8%, respectively, compared with the control HA production medium. The fmal specific volumetric productivity (0.41 g/L h) was improved by about 70% after reducing the fermentation time from 20 h to 12 h, compared to the conventional production medium.

원발성 연수 출혈증 1예

하현욱,한정호,이정석,송은향,김우정,김두응,최진영 건국대학교 의과학연구소 2000 건국의과학학술지 Vol.10 No.-

Pure primary medullary hemorrhage is a rare condition, which is characterized by ipsilateral hypoglossal nerve palsy and contralateral hemiparesis. The hemorrhage usually extended to medial and ventral medulla, these two characteristic signs distinguished from the classical presentation of Wallenberg's lateral medullary syndrome. A 53-years-old man was admitted with sudden onset left sides weakness and vertigo. Neurologic examination showed dysarthria, mild dysphagia, Horner syndrome of right side, spontaneous horizontal jerking nystagmus to left side and right deviation of tongue at protrusion. Pain and temperature sensation of left trunk, left extremity and right face were decreased. Brain CT scan and brain MRI revealed focal hemorrhage in the right medulla. We report a case of pure primary medullary hemorrhage with undetermined etiology.

흡연습관의 변화가 혈청지질변화에 미치는 영향

신용하,이종영,김진석,유재영 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Objectives : This study was conducted to assess the effect of smoking status change on the lipid profile levels and to construct fundamental data on smoking and health promotion. Methods : 581 male examinees who had taken two times of general health screening tests for aged people from 1996 till 2000 were classified into four groups - continuous non-smoker, continuous smoker, smoking stopper and new smoker and change of serum lipid profile levels between two times of tests among 4 groups were compared. Results : At the first tests, 344(59.2%) out of 581 examinees were smokers and among them, 30(5.2%) stopped smoking while among initial non-smokers, 28(4.8%) began smoking. Low-density lipoprotein cholesterol levels according to smoking status change were +6.8±27.4 for continuous non-smokers, +11.2±25.0 for continuous smokers, +10.8±28.7 for smoking stoppers, and -7.6±22.5 for new smokers (adjested by alcohol intake and obesity index, p=0.002). Differences of triglyceride levels by smoking status changes were -3.9±78.5 for continuous non-smokers, -13.6±89.6 for continuous smokers,+5.5±65.0 for smoking stoppers, +31.4±73.7 (adjusted by alcohol intake and obesity index, p=0.048) Conclusions: Although the results of this study should be interpreted in the restricted sense since there had not been effective controls over important confounding variables that might have been related to serum lipid profile levels, like changes of health belief or behavior, For smoking cessation to be more effective strategy for prevention of hyperlipidemia and atherosclerotic cardiovascular diseases, elevation of lipid profile levels and weight gain following smoking cessation should also be prevented through concomitant dietary modification and sufficient exercise.

천장관절 나사 고정술을 위한 컴퓨터 지원 수술 장치의 개발

염진섭,최원식,김하용,김환정,강종원,김영호,김형민,서동현,이석,이제범,김남국,김철영 대한골절학회 2003 대한골절학회지 Vol.16 No.1

목 적 : 천장관철의 경피적 나사 고정술을 위한 컴퓨터 지원 수술 장치를 개발하여, 그 정확도를 측정하여 보고자 하였다. 대상 및 방법 : 광학 추적 장치(Polaris, Northern Digital, Canada)와 개인용 컴퓨터를 이용한 항법 장치를 개발하였다. 정확도 측정용 팬텀을 제작하여 복합 정합시의 정합 오차와 표적 지시 오차를 각각 30회씩 측정하였으며, 4개의 플라스틱 골반골 모델(Sawbone, USA)에 16개의 6.5 ㎜유관 나사를 삽입하여 정확히 삽입되었는지 여부를 호가인하였다. 결 과 : 정합 오차는 0.76±0.33㎜, 표적 지시 오차는 1.43±0.42㎜였다. 골반골 모델에 삽입한 16개의 나사는 모두 천장관절내에 삽입되었고, 피질골을 뚫고 나오거나 인접한 두 개의 나사나 와셔가 서로 충돌한 경우는 없었다. 결 론 : 개발된 컴퓨터 지원 수술 장치는 해외에서 개발된 장치와 유사한 정확도를 가지고 있었고, 골반골 모델을 이용한 모의 수술에서도 만족할 만한 결과를 얻었다. Purpose : The purpose of this study were to develop a computer-assisted surgery system for percutaneous screw fixation of the sacro-iliac joint and to evaluate its accuracy. Materials and Methods : We have developed a navigation system composed of an optical tracking device(Polaris, Northern Digital, Canada) and a personal computer. The registration error and target localization error at hybrid registration were measured using a phantom. The errors were measured 30 times for each. Sixteen 6.5㎜ cannulated screws were inserted into four plastic bone models (Sawbones, USA), and the accuracy was evaluated. Results : The registration error was 0.76±0.33 ㎜, and the target localization error was 1.43±0.42 ㎜. All of the 16 screws were inserted well across the sacro-iliac joint, and there was neither penetration of the cortical bones nor collision between screws or washers. Conclusion : The accuracy of the developed system was similar to existing ones, and its usefulness and helpfulness was proven with screw insertion into plastic bone models.

내후성 폴리머 콘크리트의 제조 및 특성연구

연규석,김기성,이기원,황진하 강원대학교 석재복합신소재제품연구센터 2001 석재연 논문집 Vol.6 No.-

본 연구에서는 불포화 폴리에스터 수지비율을 변수로 하는 자외선 흡수제 첨가유ㆍ무 시편에 대한 Xenon lamp, 52±3℃, 실내습도 50±5%, 시험체 걸이 직경 20inches의 조건의 내후성 시험기로 각각 100, 200, 400, 800, 1600 시간 인공촉진열화 시킨 시편의 색차변화를 측정하여 불포화 폴리에서터 수지량 및 자외선 흡수제의 첨가에 의한 열화특성을 고찰하였다. 그 결과 100% 불포화 폴리에스터 수지량 및 자외선 흡수제의 첨가에 의한 열화특성을 고찰하였다. 그 결과 100% 불포화 폴리에스터 수지 시편과 상대적으로 수지비율이 큰 폴리머 페이스트에서는 자외선 조사시간에 따른 큰 색차변화 및 자외선 첨가에 의한 열화방지 효과를 확인 할 수 있었고 불포화 폴리에스터 수지비율이 상대적으로 작은 폴리머 모르타르에서는 자외선 조사에 의한 색차변화 및 자외선 흡수제 첨가에 의한 열화방지가 그리 크지 않음을 확인할 수 있었다. 이러한 것은 야외에 설치되는 폴리머 모르타르(콘크리트) 제품은 자외선에 의한 열화가 그리 크지 않음을 보여주는 결과이기도 하다. This study was to investigate the characteristics of deterioration of polymer concrete due to wethering Two sets of specimens with a various mixing ratio of unsaturated polyester resin, UV absorber added and not-added, were artificially exposed by the weathering test machine to ultraviolet (UV) radiation 100, 200, 400, 800 and 1600 hours under the conditions that the temperature was set by Xenon lamp at 52±3℃, the chamber humidity at 50±5%, and specimen rack diameter 20 inches to measure the color change to examine the characteristics of deterioration. Color difference increased as the exposure time to UV radiation increased, but decreased 20-30%, comparing to the case of non-absorber added, as the ultraviolet absorber was added. On the other hand, the effect of addition of ultraviolet absorber decreased as addition of aggregate increase. Thus, the effect of addition of ultraviolet absorber was a little on the polymer concrete containing aggregate, Especially, increment of color difference in polymer mortar specimen was lower than that of polymer resin and polymer paste specimen, when the resin volume for specimen mixture proportion was below 30%, as fine aggregate increased. As a result, outdoor-installed polymer concrete product is believed to be hardly affected by ultraviolet radiation.

Polystyrene Beads를 사용한 경량 폴리머 시멘트 모르타르의 기초적 성질

연규석,신영수,이윤수,이기원,황진하 강원대학교 부설 석재복합신소재 제품연구센터 2000 석재연 논문집 Vol.5 No.-

polystyrene beads를 사용한 경량 시멘트 모르타르의 성질을 개선하기 위해 EVA혼화용 폴리머를 첨가한 모르타르를 제조하여, 물리ㆍ역학적 성질을 살펴보았던 바 polystyrene beads 골재 재부의 공극 때문에 높은 공기량을 보였고, 플로우 값은 폴리머-시멘트비 10%에서 가장 높은 값을 나타냈다. 또한 폴리머-시멘트비가 증가할수록 압축강도와 휨강도는 증가하였고, 시멘트-경량골재비가 낮을수록 압축강도는 증가하였으나, 휨감도에는 크게 영향을 주지 않았다. The objective of this study was to improve performance of lightweight polymer cement mortars by treating with redispersible polymer powders. Lightweight polymer-cement mortars were prepared with respect to different polymer-cement ratios, and tested for water absorption, specific gravity, flexural and compressive strengths, workability and freeze-thaw resistance. Manufactured lightweight polymer-cement mortars in this study showed lower specific gravity and lower thermal diffuseness than ordinary cement mortars. Water absorption, specific gravity, flexural, compressive strengths and workability were measured according to appropriate KS test methods. As a results, the optimum mix ratios for cement-aggregate, polymer-cement, and water-cement were found to be 1:3, 10% and 30%, respectively.

저수축형 폴리머 콘크리트의 제조 및 물리·역학적 특성

연규석,이윤수,최동순,정경현,황진하 강원대학교 부설 석재복합신소재 제품연구센터 2000 석재연 논문집 Vol.5 No.-

본 연구에서는 폴리머 콘크리트의 수축저감을 목적으로 수지량, 수축저감제함량과 S/a 비등을 변화시켜 경화수축의 변화와 강도 특성을 실험적으로 구명하였다. 경화수축량은 수치량이 105%일 때 보다 11.5%일 때, 즉 수지량이 증가할수록 커짐을 알 수 있었다. 수축저감제의 첨가에 따른 경화수축량은 감소함을 알 수 있었다. B10.5-SR10일 경우 수축저감제가 첨가되지 않았을 때 보다 약 30%, B10.5-SR20 일 경우 약 53%, B11.5-SR10일 경우 약 31%, B11.5-SR20 일 경우 약 47% 정도의 수축저감의 효과를 볼 수 있었다. S/a 비에 따른 경화 수축챨ㅇ은 S/a 60일 때 가장 적은 값을 보였으며, S/a 50, S/a 40 일때는 S/a60 일 때 가장 적은 값을 보였으며, S/a 50, S/a 40일 때는 S/a 60일 때 보다 각각 10%, 30% 정도 수축량이 증가함을 알 수 있었다. 압축 및 휨 강도특성은 수지량이 증가할수록 높아지고, 수축저감제의 첨가량이 증가할수록 낮아짐을 알 수 있었다. S/a 비에 따른 강도 특성은 압축강도와 휨강도 모두 S/a 비가 60일 때 가장 큰 값을 보였으며, S/a 비가 50,40 순으로 감소하였다. Many different polymer based concrete materials are known today, but the large setting or hardening shrinkage of polymer concrete is a problem to overcome in their practical applications. The setting shrinkage reaches about five to ten times the drying shrinkage of ordinary cement concrete, i.e, 50 to 60 ×10^4. This paper deals with a reduction in the hardening shrinkage of unsaturated polyester resin concrete which is treated with respect to shrinkage-reducing agent content, S/a ratio and catalyst content, and tested for length change during hardening, and flexural and compressive strengths. It is shown that the change of shrinkage-reducing agent content and S/a ration the length change of the unsaturated polyester resin concrete during hardening.

만성 세뇨관 간질성 신염을 보인 메칠말로닌산혈증 1예

이진석,구영희,하태선 대한신장학회 2003 대한신장학회잡지 Vol.22 No.6

정신재활 치료가 정신분열병 환자의 재발율, 입원횟수, 입원일수 그리고 치료 경비에 미치는 효과 : 2년 추적조사 연구

조진석,공지현,김진원,심경순,하준선,김철권,강동호,장정희,변원탄 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

목 적 : 사회기술훈련, 환자교육, 가족교육, 집단치료, 예술치료, 작업치료와 같은 포괄적인 정신재활 치료를 약물치료와 병행할 경우 약물치료만 시행할 때에 비해 정신분열병 환자의 재발율, 입원횟수, 입원일수, 직접 치료경비 등에 어떤 효과가 있는 지를 알아보기 위함이다. 연구방법 : 1993년 9월부터 1995년 2월까지 양산 신경정신병원의 폐쇄병동에서 퇴원한 20∼35세 사이의 정신분열병 환자 173명을 대상으로 46명에게는 6개월동안 정신재활 치료를 시행하였고 127명에게는 통상적인 외래치료만 시행한 후 연구시작 시점을 포함하여 2년동안 양군에 속한 환자들을 대상으로 재발율, 입원횟수, 입원일수, 직접 치료경비 등을 1년 단위로 추적 조사하였으며, 연구시작 시점을 기준으로 전과 후의 매년 평균 입원횟수, 입원일수, 직접 치료경비도 서로 비교하였다. 결 과 : 정신재활 치료를 약물치료와 병행할 경우 약물치료만 시행할 때에 비해 재발율, 입원횟수, 입원일수, 직접 치료비용이 현저히 감소되었다. 그리고 정신재활 치료를 받은 군에서는 치료 전에 비해 치료 후에 재발율, 입원횟수, 입원일수, 직접 치료비용이 유의하게 감소하였지만 통상적인 외래치료만 받은 군에서는 치료 전과 후간에 유의한 변화를 보이지 않았다. 결 론 : 정신분열병 환자들에게 정신재활 치료를 약물치료와 병행할 경우 약물치료만 시행할 때에 비해 환자의 임상적 경과를 호전시킬 뿐 아니라 가족의 경제적 부담도 줄일 수 있다. Objective : We evaluated the clinical efficacy of a comprehensive psychiatric rehabilitation of schizophrenia, including family psychoeducation, patient education, social skills training, group psychotherapy, art therapy, and occupational therapy. Method : From September 1993 to February 1995, 173 chronic schizophrenics who met DSM-IV criteria and were discharged from a locked ward were assigned into the psychiatric rehabilitation service group(psychiatric rehabilitation group, n=46) or the customary out-patient service group(out-patient group, n=127) prospectively, but not randomly. Assignment was based on preferences of patients and their relatives, Each subject included in the psychiatric rehabilitation group received twice weekly patient education, social skills training, family psychoeducation, group psychotherapy, art therapy, occupational therapy and maintenance chemotherapy during a 6-month period. Those in the out-patient group received individual supportive psychotherapy and maintenance chemotherapy according to their clinical needs for 10-20 minutes once per week. After entry into the study, all subjects were assessed for the number of readmission, days of hospitalization, and the direct treatment costs through the first and second year of follow-ups. These outcome variables were also compared pre-and post-2 years at the time point of entry into the study. Aftercare chemotherapy on a weekly to monthly basis continued for the remainder of the two year follow-up period. Results : With regard to the mean number of hospital admissions per year, the mean days of hospitalization per year, and the direct treatment costs per year, the psychiatric rehabilitation group had significantly decreased all clinical outcomes 2 years after compared with before receiving psychiatric rehabilitation. But there were no significant differences in the all clinical outcomes between before and after study intake in the out-patient group. For the number of relapse regardless of drug compliance, 4 patients(9%) during first year and 14 patients(30%) during second year were relapsed in the 46 subjects of the psychiatric rehabilitation group. In contrast, 75 patients(59%) during first year and 90 patients (71%) during second year relapsed in the out-patient group. When considering the effect of drug noncompliance to relapse, 4(9%) and 14 patients(30%) were relapsed while on medication respectively during the first and second year in the 46 of the psychiatric rehabilitation group, vs. 27(34%) of 79 patients on medication during the first year and 31(46%) of 68 patients on medication during the second year were relapsed in the out-patient group. Conclusions : These results indicate that the comprehensive psychiatric rehabilitation can be useful therapeutic intervention both to improve the clinical outcomes of schizophrenic patients and to reduce the economical burden of their relatives.

건강질단 질병 유소견자들의 순천향구미병원 외래이용에 영향을 미치는 요인

김진석,우극현,함정오,유재영,최태성,하봉구,윤성용 순천향의학연구소 2002 Journal of Soonchunhyang Medical Science Vol.8 No.1

Background ; The purpose of routine health screening of employee is not only to detect worker's disease at early stage but also to provide early appropriate health service. But, majority of patients who diagnosed by routine health screening haven't underwent health service utilization. Soonchunhyang Kumi Hospital had been provide total occupational health service in kumi industrial estate including health screening, worker interview at workplace by occupational nurses and doctors. Object ; We want to know how much proportion of patient who are diagnosed by routine health screening had visited Soonchunhyang Kumi hospital out-patient clinic and factors influencing such health care service utilization. Method ; Initial study subject were 189 workers who had proved to have hypertension, diabetes mellitus, hyperlipidemia in routine health screening which performed during year of 2000. Among those, 160 workers who had finished questionnaire were selected for final study subject. We investigate whether they had visited health services or not, if they had, what kind of health care facility they visited, and other factors which influencing their health service utilization. Result ; Among 160 workers, 59(36.9%) had visited Soonchunhyang Kumi hospital, 47(29.4%) had visited other health care facility, and 54(33.8%) had not went to any health care facility. Factors which influencing Soonchunhyang Kumi hospital utilization is sex, job, staff's support to hospital visit during worktime, history of medical consultation about health screening result.