Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2차병원에서의 소아과 환자의 응급실 및 외래 이용에 관한 연구

김진희,배진호,전진호,문정웅 인제대학교 2006 仁濟醫學 Vol.27 No.-

Objectives : To analyze the characteristics of the pediatric patients who visited the emergency room and outpatient department of secondary hospital in order to properly guide pediatric patients to use emergency room and raise the efficiency Methods : The authors investigated and analyzed sex, age, visit day, visit time, length of stay, hospitalization and diagnostic entities of the pediatric patients who visited the emergency room and outpatient department of Wallace Memorial Baptist Hospital from January 2003 to December 2005 in a retroactive approach with their medical records. Results : For the three years, the number of total patients who visited the emergency room was 56,051 and among them, the number of pediatric patients was 7,490 (13.4%). The age group of 1 to 2 years was the largest with 2,488 (33.2%). The male to female ratio was 1.34:1. Sunday was the day of the week that the largest number of patienst visited with 2,375 (31.7%) and nearly half (48.4%) of the patients visited the emergency room during the weekend. The time when the most patient visited the emergency room was between 8 pm to 12 am. Regarding the length of stay, the highest number of patients (65.0%) stayed in the emergency room for on hour or less, and the average length of visit was 1.5 hours, which was rather short. Most patients (68.8%) left the hospital after taking simple physical examination and procedure(prescription). The most frequent diagnoses for the pediatric patients who visited the emergency room were acute pharyngitis (26.0%), acute gastroenteritis (20.7%), fever (6.6%). During the same period, the total number of pediatric patients who visited the outpatient department was 125,513. The age group of 3 to 5 was the largest with 36,041 (28.7%). The male to female ratio was 1.34:1. 6.0% of total outpatients required treatment in a hospital. Most frequent diagnoses of the outpatients were asthma (24.4%), pneumonia (12.0%), vaccination (8.7%), febrile convulsion (8.4%), acute pharyngitis (6.6%), acute gastroenteritis (5.8%). Conclusion : It is considered that a large number of pediatric patients still have used emergency room for treatment of simple and mild diseases instead of visiting outpatient department and/or primary hospital. Thus further efforts are required to properly guide pediatric patients to use emergency room and to raise the efficiency of their use.

한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률

조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.

신규 방사성 항암제 DW-166HC 의 소핵시험

문은이(Eun Yi Moon),이진(Jin Lee),이원용(Won Yong Lee),최청하(Chung Ha Choi),이덕근(Dug Keun Lee),유제만(Jei Man Ryu),정용호(Yong Ho Chung),윤성준(Sung June Yoon),박경배(Kyung Bae Park) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.3

DW-166HC (^(166)Holmium (^(166)Ho)-Chitosan complex) is a new radiopharmaceutic anticancer agent with a broad anti-tumorigenic spectrum, especially against human hepatic cancer. DW-166HC was evaluated for the appearance of micronucleus in polychromatic erythrocytes (PCEs) of mouse bone marrow cells after subcutaneous arid intravenous single administration. Bone marrow cells were prepared at 24 hr and 48 hr after DW-166HC-I (^(165)Ho-Chitosan complex : cold compound) administration and at 24 hr, 72 hr and 2 weeks after DW-166HC (^(166)Ho-Chitosan complex : hot compound) administration. The results showed there was no statistically significant increase of the numbers of PCEs with micronucleus in all DW-166HC-I administered groups compared with a negative control group but there was statistically significant increase of the numbers of PCEs with micronucleus at 24 hr arid 72 hr in all DW-166HC administered groups, which was recovered after 2 weeks from the drug administration. The results also showed the ratio of normochromatic erythrocytes (NCEs) to PCEs of all DW-166HC-I administered groups was not significantly different from that of a negative controi group but there was significant difference of this ratio at 24hr and 72 hr in all DW-166HC administered groups compared with that of negative group, which was also recovered after two weeks from the drug administration. These results suggested that DW-166HC-I may not cause any chromosomal damage but DW-166HC has in vivo mutagenic potential because of its radioactivity.

백서에서 Lidocaine에 의해 유발된 전신 경련에 대한 Baclofen의 효과

배진호,윤석화,최세진 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.1

In the rats, we compared with the onset time and convulsed number on the generalized tonic convulsion between the control group that was administrated only lidocaine and the experimental group that was premedicated with baclofen, specific GABA_8 receptor agonist, prior to lidocaine injection. The results were as follows: 1. In the control groups, according to increasing lidocaine dose, it was shortened the onset time and increased the convulsed number on the generalized tonic clonic convulsion induced by intraperitoneal lidocaine. 2. In the experimental groups, according to increasing of baclofen dose, it was delayed the onset time and decreased the convulsed number on the generalized tonic clonic convulsion induced by intraperitoneal lidocaine. These results support the facts that baclofen, specific GABA_B receptor agonist, has an anticonvulsant effect on the generalized tonic clonic convulsion induced by local anesthetics.

DiffServ 환경에서 Mobile Xcast 지원에 관한 연구

배진호,김태수,이광휘 국립7개대학공동논문집간행위원회 2003 공업기술연구 Vol.3 No.-

In the third generation of mobile computing environment, the applications that are compatible with small members of group in which the applications join the multicast sessions such as Internet phone, video conference and networked games are more prevalent. However, since the traditional multicast mechanisms supporting the mobile computing are suitable for large members of group in the multicast session, we need to consider a new mechanism to complement them. Therefore, in this paper we suggest a new multicast model that adopted explicit multicast(xcast), which has more gradual deployment with legacy Internet backbone. This proposed model is cost-effective and more scalable and guarantees the QoS in mobile computing.

음악감상이 수술실에서 마취전 환자의 불안에 미치는 영향

배진호,박종국 충북대학교 의학연구소 2000 忠北醫大學術誌 Vol.10 No.1

연구목적: 수술을 받기 위해 대기하고 있는 대부분의 환자들은 마취전에 심한 불안을 느낀다. 이러한 불안은 마취전투약과 수술 중 필요한 마취제의 투약에도 영향을 미칠 수 있으며 또 수술후의 통증에도 영향을 미칠 수 있다. 음악의 청취는 사람의 마음을 안정시켜주는 역할을 하는 것으로 알려져 있는데 이러한 음악 감상이 수술전환자의 경우에 어떠한 영향을 미칠 수 있는가 보기 위해 본 연구를 수행하였다. 대상 및 방법: 80명의 환자를 무작위로 분류하여 음악을 듣는 군(group Ⅰ)과 듣지 않는 군(Group Ⅱ)으로 분류하였다. 병실에서 혈역학적 지표를 측정하고 1군은 환자가 선택한 음악을 듣게하였다. 수술실에 막 도착하였을 때와 마취를 시작하기 직전에 환자의 불안 점수를 측정하였다. 수술 후 이삼일째에 음악에 대한 환자의 만족도를 측정하였다. 결과: Ⅰ군의 수축기 혈압이 Ⅱ군의 불안 점수는 Ⅱ군에 비해 의미있게 낮았다. 결론 : 음악 청취는 마취전 환자의 불안을 감소시키는데 효과적으로 사용될 수 있을 것이다. Purpose: Most surgical patients experience preoperative anxiety. This anxiety can effect postoperative pain. Music has been recognized as a method to reduce anxiety and fear. The effects of music listening on the preanesthetic patient's anxiety in the operating room were studied. Materials and Methods: 80 patients were randomly divided into two groups; music listening group(Group Ⅰ) and non-listening group(Group Ⅱ). At ward, hemodynamic variables including systolic and diastolic blood pressur pressure and Pulse rate were measured as control values. Then Group I patients started to listen the music selected by patients. Hemodynamic values and measurements of anxiety score with Hamilton anxiety rating scale Were measured at two times; just arrival at operating room and just before anesthetic induction. Also, patient's response to the music was measured on the postoperative 2-3rd day. Reaults : There were no significant changes in terms of systolic pressure, diastolic pressure and pulse rate except the systolic pressure that is greater than that of controls in Group Ⅱ patients. In Group I patients, anxiety score was significantly lower than that of control(10.5±3.7 vs 4.4±3.1, 5.1±3.9). At post-music, anxiety score in Group I showed significant reduced compared with Group Ⅱ (p<0.05) Patients showed relatively good satisfaction with music in both groups. Conclusion : The results suggest that music therapy was effective to reduce preanesthetic anxiety.

Mn-Zn Ferrite에서 燒成雰圍氣가 磁氣性質에 미치는 影響

裵晋鎬,朴元住 嶺南大學校 工業技術硏究所 1981 연구보고 Vol.9 No.2

The quality factor and A.C initial permeability for various sintering, cooling atmosphere conditions and adding components were measured to prepare ferrites. In this paper, the magnetic properits of Mn-Zn ferrites prepared by this method with various sintering and cooling atmospheres are discussed. The experimental results indicate that the best property in a curve of the A.C initial permeability occurs when the specimen was sintered in the nitrogen atmosphere and cooled in the air atmosphere with 0.7 [wt%] of cobalt addition. The best quality factor is also revealed with the above conditions. The A.C initial permeability increases with a large amount of N₂ gas in the cooling atmosphere, but the quality factor decreases.

Linear Stepping Motor의 驅動方式에 대한 特性考察

裵晋鎬,李相鎬,李相錫 嶺南大學校 工業技術硏究所 1988 연구보고 Vol.16 No.1

This paper has shown that it is possible to improve the dynamic characteristics and position accuracy in Linear stepping Motor by the exciting methodes. The experimental results of 1-2øexciting circuit are as follows: 1) Comparing with 2ø exciting circuit maximum starting pulse rate, maximum slewing pulse rate, and dynamic thrust were respectively increased 30%, 15% and 5%. 2) Comparing with 2ø exciting circuit improved position Accuracy.

C6 glia 세포에서 유도성 Nitric Oxide Synthase 유전자 발현조절에 관한 연구

배진영,허강민,배소현,박지선,이충재,이재흔,석정호 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

To investigate transcriptional regulation of iNOS gene by LPS and cytokines, the production of NO, expression of iNOS mRNA and protein, binding activity of nuclear factor-kappa B(NF-kB), and promoter activity of iNOS gene were examined in rat C6 glial cells. LPS, interferon-gamma(IFN-γ), and tumor necrosis factor-alpha (TNF-α) stimulated the production of NO, which was increased synergistically by co-treatment. By the treatment of LPS, iNOS mRNA expression was initiated at 1 h, markedly increased by 3 h, and decreased gradually afterward. iNOS mRNA expression was markedly enhanced by mixture of LPS, IFN-γ and TNF-α. iNOS protein synthesis was increased by the treatment of mixture LPS and cytokine mixture. Treatment of LPS stimulated NF-kB activation, and the activation reached to the maximum level at 30 min, and the treatment of mixture of LPS and cytokines increased the activation. To determine the effect of NF-kB binding activity on iNOS promoter activation, CAT assay was performed. iNOS promoter activity was increased by the treatment with LPS for 5.5 h, and further increased by the combined treatment with LPS and cytokines. These results suggest that NF-kB activation by LPS and cytokines may play a significant role in the induction of the iNOS gene.