Ki - 1 임파종 1 예

강민모(Min Mo Kang),김경재(Kyoung Jae Kim),이지현(Ji Hyun Lee),최석문(Seog Mun Choi),안기성(Ki Sung Ahn),황기석(Kee Suk Whang),김용진(Yong Jin Kim),박재복(Jae Bok Park) 대한내과학회 1993 대한내과학회지 Vol.45 No.4

A 60-year-old man was admitted to our hospital complaining of fever with chillness and anarexia for 12 weeks. Physical examination revealed supraclavicular lymphadenopathy and left inguinal lymphadenopathy. Abdominal CT scan showed the evidence of maked lymphadenopathy on retraperitoneal, external iliac, obturator, left inguinal, and celiac lymph nodes. A pathologic diagnosis of Ki-1(+) lymphoma was made by the biopsed supraclavicular lymph node because the node consisted of large cells with pleomorphic nuclei, scanty cytoplasm, large basophilic nucleoli, atypical vacuoli and large cells were positive for leukocyte common antigen, Ber-H2, marker, and B-cell marker.

Effects of High Cholesterol Diet on Newly Generated Cells in the Dentate Gyrus of C57BL/6N and C3H/HeN Mice

KIM, Il Yong,HWANG, In Koo,CHOI, Ji Won,YOO, Ki-Yeon,KIM, Yo Na,YI, Sun Shin,WON, Moo-Ho,LEE, In Se,YOON, Yeo Sung,SEONG, Je Kyung Japanese Society of Veterinary Science 2009 The Journal of veterinary medical science Vol.71 No.6

<P>In this study, we observed and compared the effects of a high cholesterol diet (HCD) on cell proliferation and differentiation in the subgranular zone of the dentate gyrus of C57BL/6N (B6, susceptible strain) and C3H/HeN (C3H, resistant strain) mice. Ki67 (a marker for cell proliferation) positive cells) were significantly decreased in HCD-fed B6 mice compared to those in B6 (49.7%) and C3H mice fed a low cholesterol diet (LCD). In addition, doublecortin (DCX, a marker for cell differentiation or neuroblasts)-immunoreactive cells in HCD-fed B6 mice were significantly decreased compared to those in LCD-fed B6 and C3H mice. These results suggest that B6 strains are sensitive to HCD, which impairs cell proliferation and differentiation.</P>

Dietary fat increases solid tumor growth and metastasis of 4T1 murine mammary carcinoma cells and mortality in obesity-resistant BALB/c mice

Kim, Eun Ji,Choi, Mi-Ran,Park, Heesook,Kim, Minhee,Hong, Ji Eun,Lee, Jae-Yong,Chun, Hyang Sook,Lee, Ki Won,Yoon Park, Jung Han BioMed Central 2011 Breast cancer research Vol.13 No.4

<P><B>Introduction</B></P><P>High-fat diets (HFDs) are known to cause obesity and are associated with breast cancer progression and metastasis. Because obesity is associated with breast cancer progression, it is important to determine whether dietary fat <I>per se </I>stimulates breast cancer progression in the absence of obesity. This study investigated whether an HFD increases breast cancer growth and metastasis, as well as mortality, in obesity-resistant BALB/c mice.</P><P><B>Methods</B></P><P>The 4-week-old, female BALB/c mice were fed HFD (60% kcal fat) or control diet (CD, 10% kcal fat) for 16 weeks. Subsequently, 4T1 mammary carcinoma cells were injected into the inguinal mammary fat pads of mice fed continuously on their respective diets. Cell-cycle progression, angiogenesis, and immune cells in tumor tissues, proteases and adhesion molecules in the lungs, and serum cytokine levels were analyzed with immunohistochemistry, Western blotting, and enzyme-linked immunosorbent assay (ELISA). <I>In vitro </I>studies were also conducted to evaluate the effects of cytokines on 4T1 cell viability, migration, and adhesion.</P><P><B>Results</B></P><P>Spleen and gonadal fat-pad weights, tumor weight, the number and volume of tumor nodules in the lung and liver, and tumor-associated mortality were increased in the HFD group, with only slight increases in energy intake and body weight. HF feeding increased macrophage infiltration into adipose tissues, the number of lipid vacuoles and the expression of cyclin-dependent kinase (CDK)2, cyclin D1, cyclin A, Ki67, CD31, CD45, and CD68 in the tumor tissues, and elevated serum levels of complement fragment 5a (C5a), interleukin (IL)-16, macrophage colony-stimulating factor (M-CSF), soluble intercellular adhesion molecule (sICAM)-1, tissue inhibitors of metalloproteinase (TIMP)-1, leptin, and triggering receptor expressed on myeloid cells (TREM)-1. Protein levels of the urokinase-type plasminogen activator, ICAM-1, and vascular cell adhesion molecule-1 were increased, but plasminogen activator inhibitor-1 levels were decreased in the lungs of the HFD group. <I>In vitro </I>assays using 4T1 cells showed that sICAM-1 increased viability; TREM-1, TIMP-1, M-CSF, and sICAM-1 increased migration; and C5a, sICAM-1, IL-16, M-CSF, TIMP-1, and TREM-1 increased adhesion.</P><P><B>Conclusions</B></P><P>Dietary fat increases mammary tumor growth and metastasis, thereby increasing mortality in obesity-resistant mice.</P>

한국산 자리공 자리공 열매의 적색소를 이용한 식용색소 : Ⅱ. 활성 사포닌을 제거한 색소의 안정성과 가공적성 Ⅱ. Color Stability and Characteristics as Food Colorant of Saponin-Free Pokeberry Pigments

박용석,서지연,황혜정,박기환,윤광로 中央大學校 食糧資源硏究所 2003 食糧資源硏究所 論文集 Vol.15 No.1

우리나라산 자리공 열매의 색소 함량은 산지에 따라 건물 g당 48.56에서 62.16mg으로 천연색소 자원으로 높은 편이였으며 산지간의 격차가 크지 않아 자원성이 좋은 편이었ㄷ. 자리공 열매의 적색소를 absolute ethanol 침전법으로 분획하는 과정에서 활성 사포닌이 95% 이상 제거되며 이 색고 침전물을 silica gel column이나 Sephadex G-25 column으로 정제함으로서 색소의 순도를 높일 수 있었다. Absolute ethanol 처리와 column 정제법을 응용하여 활성 사포닌을 제거 한 실험용 자리공 열매색소 분획 모두 색소 안정성이나 가공적성에서 뚜렷한 차리를 보이지 않았다. 따라서 absolute ethanol만 처리한 색소제품이나 이를 column chromatography로 정제한 색소분획 모두 고순도의 천연색소 첨가물로의 응용 가능성은 분명하였다. 이들 활성 사포닌을 제거한 자리공 열매색소의 색소안정성과 가공적성은 다음과 같이 요약 할 수 있다. 즉, pH 안정범위는 4.8-6.0이었으며, 열에 대한 안정성을 조사한 결과는 4℃에서 45일 저장했을 때는 70%의 색소 잔존율을 나타낼 정도로 안정한 편이었지만 25℃에서는 10일째에서 70% 잔존율을, 50℃이상의 온도에서는 수 시간 정도에서 50%이하의 색소 잔존율을 보여 전반적으로 열 안정성은 불량한 편이었다. 한편 이들의 존재 하에서는 급격한 변색이 진행되었다. 자리공열매 색소에 대하여 ascorbic acid나 palmityl ascorbate의 첨가는 색소 잔존율에 큰 영향을 미치지 않았으며 chlorogenic acid, resocinol 그리고 catechin의 첨가도 마찬가지였다. 포도당, 과당, 맥아당, 설탕, 펙틴, 셀루로스 그리고 cyclodextrin 등의 탄수화물도 전혀 영양을 미치지 않았다. 1000ppm 이내의 EDTA나 citric acid, malic acid 또는 oxalic acid 등의 산도 미치지 않았다. 금속이온으로서, Ca^(2+)과 Al^(3+)의 첨가는 색소의 잔존율에 큰 영향을 미치지 않았으나, Cu^(2+)나 Fe^(2+)의 첨가는 심한 변색을 초래하였다. 자리공 열매즙의 무수 알코올 처리물(적색 색소 침전물)은 soft gel, firm gel 또는 캔디 등의 고형 식품에 잘 어울리고, 음료에서는 변색이 빠르게 진행되며, 특히 요구르트의 경우가 가장 민감하였다. 결론적으로 보면, 우리나라산 자리공 열매에서 활성 사포닌을 제거하고 확보한 색소제품은 사탕무의 betanin과 거의 같은 색소안정성과 가공적성을 보여 줌으로서 사탕무색소 첨가제인 beet red를 대신할 수 있으며 색소의 함량이나 순도라는 관점에서는 훨씬 우수할 것으로 사료된다. To evaluate the basic data on the color stabilities of saponin-free pokeberry pigment products, % remain of pigment were calculated from absorbances of solutions stored at various conditions. The pigment products were estimated to be very stable at pH 4.8-6.0. Thermostability of pH 5.0 solution of pigment products could be kept stable below 4℃. Addition of Cu2+ or/and Fe2+ decreased the % remain of pigment, whereas most food components had no offset the stability of pokeberry pigment. The color changes of some model foods having pokeberry pigment were checked by Hunter color differerence meter for 45 days. In solid foods like gels and candy the changes of color difference were very slower than that of model beverages. Application of pokeberry pigment to soft drink and yogurt were found to be effective at the refrigerated condition.

정상임신 산모와 중증자간전증 산모의 혈소판지수 비교

박용범,한지수,정동근,이인식,김암,민원기,지현숙,목정은 울산대학교 의과대학 1993 울산의대학술지 Vol.2 No.1

The purpose of this retrospective study was to define platelet indices in severe preeclampsia(N=98) and to compare them to normal nonpregnant values(N=93) during the years 1990-1993 in the Department of Obstetrics and Gynecology, College of medicine, University of Ulsan, Asan Medical Center. Indices evaluated included platelet count, mean platelet volume, and platelet distribution width. Means platelet volume was statistically different between two groups, however mean platelet count was significantly decreased(p=0.029) and platelet distribution width was significantly increased(p=0.0001) and platelet distribution width was significantly increased(p=0.0001) in severe preeclampria. Mean platelet volume versus platelet count in normal pregnancy showed a significant inverse nonlinear relationship(r=-0.39, 9<0.001) and that in preeclampsia was congruent with notmal nonpregnant valus. With same mean platelet volume, platelet distribution width showed tendency to shift to right in severe preeclampsia. These findings support the concept of severe preeclampsia as a compensated state of increased platelet comsumption comparing with normal pregnancy. We concluded from above results that quantification of platelet indices may have some value in screening for and following severe preeclampsia.

분당 신도시 개발 이후 용인시의 공간구조 변화에 대한 연구

조지혜,김영욱,박영기 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

The purpose of this study was to analyze quantitatively the transformation of spatial structure in the near city of a newtown after the development of it The result is that the development of a newtown had an effect on the trans formation of spatial structure in the near city. The analysis shows that Yongin city's spatial configuration with near cities became strong. And its northwest was more integrated forward Bundang new city than old urban core of it. As a result, it shows a decline of the old urban core.

중국 기업공시제도의 개선방향에 관한 연구

홍기용,박희선 한국세무회계학회 2002 세무회계연구 Vol.11 No.-

중국은 1980년대에 증권시장이 형성된 후 상장회사의 운영이 더욱 확대되었으며, 그동안 증권법을 제정하였고 관련법규들도 개정을 하는 등 각종 제도를 정비해 왔다. 이 과정에서 기업정보의 공시도 많은 발전을 가져왔지만 아직도 공시제도의 운영이나 관리가 선진국가들에 비해 미흡한 수준이다. 최근 중국은 시장경제의 발전 특히 WTO의 가입으로 국제경쟁력강화와 국제관례에 부합되도록 기존의 회계 및 경제법규들을 점진적으로 개정하여 가고 있다. 그러나 효율적 증권시장의 운영과 투자자 권익을 보호하기 위한 현행 기업공시제도에 대한 개정은 개정의 폭이나 내용의 중요성 측면에서 볼 때 부분개정의 형식에 의한 것으로서 증권법체계가 완전히 정비되지는 못하였다고 할 수 있다. 따라서 이 논문은 문헌연구를 통하여 현행 중국의 기업공시제도 문제점을 검토 및 분석하고 향후 개선방향을 아래와 같이 제시하였다. 첫째, 정부주도의 공시관리체제를 민간관리체제로 이양, 둘째, 전자매체공시의 보편화를 추진, 셋째, 공시되는 정보의 정확성을 제고, 넷째, 회계정보의 다양성 및 적시성을 확보, 다섯째, 공시책임자제도의 도입 등이다.

90°分岐管內 3次元 流動測定에 관한 硏究

김지환,김용재,김기우,유영태 朝鮮大學校 機械技術硏究所 1998 機械技術硏究 Vol.1 No.1

Heat engine fluid machinery in the plant have to linked with various ducts net and the corresponding design have to be concerned about effectiveness and stability of system of plant. To optimum control and design system concerning stability, economization, operating effectiveness, we have to exactly analysis flow properties of duct applying to fluid machinery, heat exchanger, cooling machine, air conditioning equipment. Therefore, it is necessary to research the duct, heat transfer equipment, for increasing overall effectiveness of air conditioning system by suggesting basic data of duct resulting from organic research. So we can contribute to technical developement of duct. According to the experiment on working properties, we can conclude as followings 1) The flow from the upstream of main duct outflows toward the branch duct after carring out complicated vortex flow, but the flow appearance is exptemely complex. The flux and others, 80% flux of straight duct, passed by through the main duct. 2) In case of speeding up the flow velocity of the duct, lots of wave velocity component is occured, the value of wave velocity component is large by the mixture of two boundary layers resulting from developing the boundary layer at both walls of duct.

家兎의 總輸膽管의 不完全閉鎖와 完全閉鎖가 肝機能에 미치는 影響

成志鏞,孫基燮 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

We have been encountered many troublesome and complicated problems in caring of the patients who have had a common bile duct stenosis and obstruction to intrinsic and extrinsic factors. Though numerous studies previously devoted to these problems which have relied upon experiment and practice, we are still far from the exact sequence of events. Author have worked with 78 adult rabbits of no particular strain and sex, weighing around 2kg and free from disease, feeding under same circumstances. The experimental animals have been applied partial and complete ligation of common bile ducts (C.B.D.), in order to know its influence on results of biochemical changes, chiefly liver function test. and obtained samples through cardiac puncture. The results were as follows 1. The value of serum G.P.T rose steeply on the first day about 18 times of control groups under incomplete ligation of the C.B.D. and rose to reach a plateau on 12 hours after ligation and then fell to almost same level to control group, control's levels on the 10th, 30th and 50th days 2, The serum alkaline phosphatase activity have risen to about 6 times of control group from 12 hours to the 2nd day after incomplete C.B.D. ligation and have risen to about 10 to 16 times from 12 hours to the 3rd days after complete of the C.B.D. ligation, thereafter both of them have fallen to the level of control group from the 10th day. 3. The serum albumin level has trend to decrease gradually after both incomplete and complete ligation of C. B. D. especially remarkable on the latter. 4. Almost all of serum total bilirubin level were similar to control group except the level of the 10th day's group. which was 6 times as high as control group after incomplete C.B.D. ligat ion. And druing first 10 days serum total bilirubin level have steeply risen from 13 to 22 times of control group, and then fallen to the level of control group from the 30th day after complete C.B.D. ligation. 5. All serum amylase level had rather slight fluctuation at the level of control group until the 30th day after both incomplete and complete lightion of the C.B.D. but those probably had no significant meaning. 6. There are significant meaning of interaction between duration and treatment during studying experiment on the serum G.P.T., alkaline phosphatase, albumin, and total bilirubin.

실험동물에서의 내분비계 교란물질인 DEHP, DBP의 생체내 영향

정지윤,이성호,박영석,박병권,김병수,김상기,박창식,강경선,이영순 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

The major protocol features of the rodent uterotrophic assay have been evaluated using a range of reference chemicals. The protocol variables considered include the selection of the test species and route of chemical administration, the age of the test animals, the maintenance diet used, and the specificity of the assay for estrogens. The rodents were ovariectomized under general anesthesia via bilateral flank incisions and randomly assigned to groups of 5 animals. This study investigated the estrogenic effects of phthalates, which are DEHP and DBP, suspected as endocrine disruptors. It is concluded that three daily subcutaneous administration of test chemicals, which are di-ethylhexyl phthalate(DEHP) and di-n-butyl phthalate(DBP), to 9 week-old rodents. The results revealed that DEHP & DBP show similar uterotrophic activity in the rat and the mouse, using subcutaneous administration. DEHP and DBP failed to affect any of these parameters, which are body weight, mammary gland, vaginal and uterus histopathology. but, DBP increased uterus weight to only one rat of 1.0 g/kg group. The results conform the need for rigorous attention to experimental design and criteria for assessing estrogenic activity.