A selective cyclin-dependent kinase 4, 6 dual inhibitor, Ribociclib (LEE011) inhibits cell proliferation and induces apoptosis in aggressive thyroid cancer

Lee, Hyun Joo,Lee, Woo Kyung,Kang, Chan Woo,Ku, Cheol Ryong,Cho, Yoon Hee,Lee, Eun Jig Elsevier 2018 Cancer letters Vol.417 No.-

<P><B>Abstract</B></P> <P>The RB-E2F1 pathway is an important mechanism of cell-cycle control, and deregulation of this pathway is one of the key factors contributing to tumorigenesis. Cyclin-dependent kinases (CDKs) and Cyclin D have been known to increase in aggressive thyroid cancer. However, there has been no study to investigate effects of a selective CDK 4/6 inhibitor, Ribociclib (LEE011), in thyroid cancer. Performing Western blotting, we found that RB phosphorylation and the expression of Cyclin D are significantly higher in papillary thyroid cancer (PTC) cell lines as well as anaplastic thyroid cancer (ATC) cell lines, compared with normal thyroid cell line and follicular thyroid cancer cell line. LEE011 dose-dependently inhibited RB phosphorylation and also decreased the expressions of its target genes such as <I>FOXM1, Cyclin A1,</I> and <I>Myc</I> in ATC. Furthermore, LEE011 induced cell cycle arrest in G0-G1 phase and cell apoptosis, and inhibited cell proliferation in ATC. Consistently, oral administration of LEE011 to ATC xenograft models strongly inhibited tumor growth with decreased expressions of pRB, pAKT and Ki-67, and also significantly increased tumor cell apoptosis. Taken together, our data support the rationale for clinical development of the CDK4/6 inhibitor as a therapy for patients with aggressive thyroid cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> pRB and Cyclin D were expressed high in aggressive thyroid cancer. </LI> <LI> LEE011 suppressed pRB and also decreased the expressions of its target genes in ATC. </LI> <LI> LEE011 induced cell cycle G1 arrest and apoptosis, and inhibited cell proliferation. </LI> <LI> LEE011 inhibited in vivo tumor growth with decreased expressions of pRB and Ki-67. </LI> <LI> We could explain the anticancer effects with the RB-E2F pathway. </LI> </UL> </P>

항인지질 항체 증후군에서 항β₂glycoprotein-Ⅰ 항체의 의미

이찬희,서창희,이지수,이수곤 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

objective : This study investigated the significance of anti-β₂ glycoprotein-Ⅰ(β₂GPI) antibody in antiphospholipid syndrome from the results of the relationship between the antiphospholipid antibody test and the anti-β₂GPI antibody test. Methods : The IgM, IgG type of the anti-β₂GPI antibody was tested by ELISA in patients with antiphospholipid syndrome or those who were suspected of having antiphospholipid syndrome and compared with the anticardiolipin antibody and lupus anticoagulant results. As a control, the anti-β₂GPI antibody was tested in 12 normal healthy persons and 8 systemic lupus erythematosus patients. Results : In 6 patients with antiphospholipid syndrome, the positive rate of lupus anticoagulant, IgM and IgG anticardiolipin antibody was 33%, 50%, and 67%. However, the positive rate of the IgM and IgG anti-β₂GPI antibody test was 83%, 100%. Among the 6 patients, 3 patients were tested for the anti-β₂GPI antibody before and after treatment. In all 3 patients, the anti-β₂GPI antibody titer was lower after treatment. The anti-β₂GPI antibody test was positive in those patients suspected for having antiphospholipid syndrome, but tested negative to the anticardiolipin antibody or lupus anticoagulant. In the normal and disease controls, the anti-β₂GPI antibody test was negative in all cases. Conclusion : In diagnosing antiphosphotipid syndrome. the anticardiolipin antibody or lupus anticoagulant should be used as a screening test, and if necessary, an anti-β₂GPI antibody should be added. This might improve the sensitivity and specificity. Also, the anti-β₂GPI antibody titer may be used as a marker of disease activity.

B16 멜라닌 세포에서 해양소재 추출물의 멜라닌 생성 저해 효과

이찬 ( Chan Lee1 ),장정희 ( Jung Hee Jang ),안은미 ( Eun Mi Ahn ),박찬익 ( Chan Ik Park ) 대한본초학회 2012 大韓本草學會誌 Vol.27 No.4

Objectives : Under normal condition melanin protects the skin from extracellular stimuli including ultraviolet (UV)-induced oxidative skin damages, but excess production and accumulation of melanin can induce hyperpigmentation causing esthetic problems. Therefore, in this study we tried to search for natural skin whitening materials from marine natural resources. Methods : Water and ethanol extracts of marine natural resources were prepared from Porphyra thalli (PT), Laminariae thallus (LT), Ostreae concha (OC), Sargassum thallus (ST), Undaria thallus (UT), Codium thalli (CT), Enteromorpha thalli (ET), Syngnathoides biaculeatus (SB), and Hippocampus coronatus (Hc). Their effects against UVB and α-melanocyte stimulating hormone (α-MSH)-induced melanogenesis were investigated based on melanin formation in B16 mouse melanoma cells. The mRNA and protein expression of enzymes involved in the melanogenic process were further examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. Results : Water extract of Ostreae concha (OCW/E) effectively inhibited UVB and α-MSH-induced melanin production in B16 melanocytes, which seemed to be mediated by inhibition of mRNA expression of tyrosinase and tyrosinase-related protein 1 (TRP-1). In another experiment, ethanol extracts from Porphyra thalli (PTE/E), Laminariae thallus (LTE/E), Sargassum thallus (STE/E), Undaria thallus (UTE/E), Codium thalli (CTE/E), Syngnathoides biaculeatus (SBE/E), and Hippocampus coronatus (HcE/E) significantly suppressed UVB and α-MSH-induced melanin formation. Furthermore, ethylacetate fraction isolated form LTE/E (LTE/EEt) decreased UVB and α-MSH-elevated extracellular melanin levels via inhibition of tyrosinase protein expression. Conclutions : These results suggest that marine natural resources such as Porphyra thalli, Laminariae thallus, Ostreae concha, Sargassum thallus, Undaria thallus, Codium thalli, Syngnathoides biaculeatus and Hippocampus coronatus have anti-melanogenic effects, thereby exhibiting high potentials to be utilized as one of the ingredients for the development of new whitening functional cosmetics.

Transmission ability of Zika virus with artificially infected Aedes albopictus in Korea

Yang Sung‐Chan,Lee Hee-Il,Kim Hyunwoo,Lee Wook‐Gyo 한국곤충학회 2021 Entomological Research Vol.51 No.8

Zika virus (ZIKV), a virus mainly occurring in South America, is now globally distributed. Understanding the pathogen transmission cycle in a vector is exceptionally important in developing disease control strategies. In this study, we performed ZIKV microinjection in Aedes albopictus (Skuese), to estimate its vertical and horizontal transmission ability. The virus infection rate was confirmed by real-time qPCR in the F1 generation derived from the ZIKV-injected Ae. albopictus. The minimum infection rate (MIR) of the F1 generation was highest when they were injected with the virus at 6–12 h (MIR: 16.1) and lowest at 12–24 h (MIR: 4.7) after feeding. In the developmental stage of the F1 progeny, MIR values were 1.0, 1.3, and 6.7 in each egg, larval, and adult stage, respectively, but ZIKV was not detected in the pupa stage. Virus transmission ability was not significantly different between the collection areas (Tongyeong and Jeju). Ae. albopictus demonstrated a high venereal transmission rate of ZIKV and was detected in males (6 pool/7 pool) and females (6 pool/7 pool), confirming that ZIKV can be transmitted from infected mosquitoes to uninfected mosquitoes via mating. This is the first study of the administration of a ZIKV microinjection to Ae. albopictus in Korea and suggests a possibility of a potential mechanism for the virus to survive during adverse conditions via vertical transmission.

우리나라 南西海岸의 海陸風 探究 : Centering around the city of Mokpo and Seochon

李承姬,柳贊洙 조선대학교 사범대학 부설 교과교육연구소 2000 敎科敎育硏究 Vol.3 No.1

Geographically, Three sides of Korea are surrounded by the sea(that is, the Korean Peninsular). Accordingly, the envioronment of our country has been greatly affected by the sea. For this study and taking an observation with AWS. I've selected the Mokpo city to which Taebul industrial complex is adjacent and Seochon, the central part of the western coast to which Asan industrial complex is adjacent. I've utilized the data on the change of the direction and the speed of the breeze of two areas(Mokpo and Seochon) through classifying all the data of the annual breeze into a daily unit, a monthly unit and a seasonal unit. As a result, there was a more vivid sea and land breeze character in Seochon area than in Mokpo area is due to the Mokpo's geographical location. In the frequency of breeze direction, NNW direction which occupied 27% of the whole was prevailing and in the frequency of breeze speed, the range of 0.3∼3.3㎧ appeared frequently, and it showed 48.9%. According to rarity analysis on the relations between breeze direction and breeze speed, a correlation coefficient was 0.56∼0.91 and through it. I could figure out correlation of breeze direction and breeze speed. But iti is matter of regret that this study has a statistical analytic basis mainly, so, from noe on, I intend to do profound research on factors which cause the change of sea and land breeze and I'd like to develop them to be processed as enviornmental factors.

카올린으로부터 질화알루미늄 분말 합성

이윤복,서기원,류봉기,김광호,박희찬,장윤식 釜山大學校生産技術硏究所 1996 生産技術硏究所論文集 Vol.50 No.-

카올린으로 부터 수화염기성황산알루미늄을 제조하고, 이것을 탄소열환원질화하여 질화알미늄 분말을 합성하였다. 수화염기성황산알루미늄은 카올린으로 부터 제조된 수화황산알루미나 0.5M 및 요소 5M인 용액으로 부터 얻은 것이 가장 미세하였다. 탄소열환원질화법으로 질화알미늄을 합성할 때 활성화탄과 염기성황산알루미늄의 질량비가 2인 조성이 가장 적합하였으며, 이 때 얻어진 질화알미늄은 0.3-1㎛의 submicron급 분말이었다. Aluminum nitride powder was synthesized by carbothermal reduction-nirtridation using kaolin- derived hydrated basic aluminum sulfate. The hydrated basic aluminum sulfate powder produced from the solution of 0.5M hydrated aluminum sulfate and 5M urea showed very fine particles. The aluminum nitride showed the submicron powder of 0.3-1㎛ in the optimum condition with 2.0-weight ratio of activated charcol to hydrated basic aluminum sulfate.

경주 남리사지에 사용된 석재의 암석학적 특성과 원산지 해석

이찬희,정연삼,정민호,이명성,김영택 공주대학교 문화재보존과학연구소 2004 문화재과학기술 Vol.3 No.1

이 연구는 경주 남리사지에 이용된 석재를 대상으로 암석의 성인적 분류, 광물 및 지구화학적 특성을 검토하여 석재의 기원지 추정과 고고지질학적 의미를 해석한 것이다. 남리사지에서 출토된 암석은 5종의 화강암질암 (알카리 화강암, 흑운모 화강암, 미문상 화강암, 등립질 화강암, 홍장석 화강암), 세립질 퇴적암 (셰일, 미사암), 호온펠스, 옹회암질암, 염기성 맥암 등이 있다. 이 중에서 서탑에 이용된 암석은 유백색의 중립질 알카리 화강암과 담홍색의 흑운모 화강암이 주류를 이룬다. 각 석재들의 대자율 값은 흑운모 화강암이 4.94~7.59 (평균 6.47)로서 제일 높았으며 미문상 화강암이 0.86~1.54 (평균 1.13)로 제일 낮았다. 알카리 화강암은 3.51, 홍장석 화강암이 5.88, 등립질 화강암이 4.89로서 분화경로와 산출상태에 따라 조금씩 다른 값을 보였다. 이 화강암류는 화학조성으로 볼 때, 거의 동일한 마그마 기원의 칼크-알카리 계열 화강암 영역에 해당된다. 이 석재의 화학적 풍화지수와 풍화 잠재지수는 각각 49.87~61.64와 4.12~8.46 범위로서, 현재 암석이 화학적 풍화작용이 왕성하게 발생하는 정도는 아니나 풍화잠재력은 매우 높은 것으로 나타났다. 이 화강암질암의 암석학적 및 지구화학적 특징으로 볼 때, 이들의 성인적 유형은 남산 일대에 분포하는 화강암과 동일하다. 따라서 남리사지의 석조문화재 건축에 사용된 석재는 현지에서 재료를 구하고 현장에서 제작한 자급자족형 석재와 수계의 상류지역에서 도입된 외래형 석재가 공준하고 있는 것으로 해석된다. 자급형 석재가 현지의 원료를 이용한 것은 확실하나 어느 지역에서 채석하여 어디서 가공을 하였는지는 불분명하다. Rock properties around the Namri temple site was studied source area and archaeogeological interpretations based on petrology, mineralogy and geochemistry. Excavated rocks from the area composed of granitic rocks (alkali granite, biotite granite, micrographic granite, eqigranular granite, pink feldspar granite), fine grained sedimentary rocks (shale and siltstone), hornfels, tuffaceous and basic dyke rocks. Almost rock materials of the west pagoda made of white medium grained alkali granite and biotite granite. As the magnetic susceptibility (MS), biotite granite ranges from 4.94 to 7.59 (mean 6.47), and micrographic granite is lowest value of 0.86 to 1.54 (mean 1.13). The mean MS values revealed that alkali granite = 3.51, pink feldspar granite = 5.88, eqigranular granite = 4.89, which are little variatins because of differentiation process and field occurrences. In the geochemical data, the granitic rocks represent a comagmatic calc-alkali series granite. The values of WPI and CIA for rock properties from the Namri temple site range from 49.87 to 61.64 and 4.12 to 8.46, respectively, which values don't going active weathering right now, but weathering potential represents very high. On the basis of petrology and geochemistry of the rock properties from the Namri temple site, the genetic types are the similar as those of the Namsan granite. Therefore, the using rocks of the Namri temple site suggested that domestic type rock properties getting material in the actual place and making in situ, and foreign type rock properties getting from the upstream area are coexisted. Domestic types are sure of making the actual place material, but unclear where is a quarry and working.

소세포 폐암의 갑상선전이 1예

장리라,최영식,박요한,문대성,김자경,김성은,최인수,유찬희,남성진 고신대학교 의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.3

Metastasis to the thyroid gland is usually considered uncommon. The most frequent sites of the primary tumor include the kidney, breast and lung. Its presence often indicates poor prognosis. Metastases represent the advanced stage of the tumors and fine needle aspiration cytology is and important way of diagnosis in thyroid metastasis. we experienced a case of Metastatic Small cell Lung Carcinoma to the Thyroid Gland. A 58-year-old man with Graves' disease diagnosed pulmonary nodule in the left upper lung field on his chest X-ray film. The patient's thyroid gland was diffusely swollen, and elastic to hard. A thyroid ultrasonography showed 1cm sized hypodense nodule on the left thyroid gland. The patient underwent a ultrasonography guided fine needle aspiration cytology of the mass. Cytological diagnosis was a metastatic small cell carcinoma. Then Chemotherapy was administered to the patient. The authors report this rare case of metastatic small cell lung carcinoma to the thyroid with literature review.

스포츠種目別運動選手의 體格, 體型 및 體組成에 관한 硏究

李相于,趙在基,朴贊熙,朴俊東,辛尙根 東亞大學校附設스포츠科學硏究所 1989 스포츠科學硏究論文集 Vol.7 No.-

대한신경정신의학회 학술대회의 과거, 현재, 미래

이영식,오강섭,노경선,김찬형,한진희,이민수 大韓神經精神醫學會 2003 신경정신의학 Vol.42 No.4

The Korean Neuropsychiatic Association (KNPA) was founded in 1945 and has now grown to over 2000 members as of 2003. The Association's academic meeting has also been increasing in size every year. To further develop the academic meeting of the KNPA, the task force team conducted this project. We suggest several points based on analysing the abstracts of the meeting that has conducted during the last 5 years, the survey results performed at the 2002 annual meeting, the annual meeting of the American Psychiatric Association, and the academic activities of a Korean medical assoction, the Korean Society of Otolaryngology.