Complete genome sequence of Sphingobium sp. strain PAMC 28499 reveals a potential for degrading pectin with comparative genomics approach

So‑Ra Han,Sung‑Min Jang,Young Min Chi,Byeollee Kim,정상희,Yung Mi Lee,Jun Uetake,Jun Hyuck Lee,Hyun Park,오태진 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background Spingobium sp. PAMC 28499 is isolated from the glaciers of Uganda. Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor. The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry. Objective In this study, complete genome sequence of Spingobium sp. PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region. Methods Genome sequencingwas performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology. The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI nonredundant database. And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes). Results The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons. Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases. CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae. These results confrmed that this Sphingobium sp. strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway. Conclusion In this study, isolated and sequenced the complete genome of Spingobium sp. PAMC 28499. Also, this strain has comparative genome analysis. Through the complete genome we can predict how this strain can store and produce energy in extreme environment. It can also provide bioengineered data by fnding new genes that degradation the pectin

상심실성 빈맥환자에서 관상정맥동의 형태비교

현대우,김윤년,박소영,한성욱,허승호,김기식,김권배 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.4

三天池의 環境과 微生物의 多樣性

한상국,이종운,배소현,장무웅,김시균 嶺南大學校 環境問題硏究所 1993 環境硏究 Vol.13 No.1

경상북도 경산시에 위치하고 있는 삼천지에 대한 환경요인 및 미생물상에 관하여 조사하였다. 삼천지 수계의 환경요인 분석결과, 그 평균값은 기온이 25.8℃, pH6.77, 경도 117.94mg/ℓ, 부유물질 4.5mg/ℓ, 용존산소량 3.3mg/ℓ, 생물학적 산소요구량 6.6mg/ℓ, 화학적 산쇼요구량 7.2㎎/ℓ, 염소이온 17.56㎎/ℓ, 황산이온 7.14㎎/ℓ로 나타났고, 중금속류는 Mn과 Zn만이 소량 검출되었으나 전반적인 오염도가 높아 수질환경 기준 IV급수에 해당하였다. 총 세균수는 3.32-8.85×?? No. inds/㎖의 분포를 나타냈고, 식물플랑크톤의 종구성은 규조류 33종, 녹조류 26종, 남조류 8종, 유글레나 13종, 와편모조류 6종 등 86종이 확인되었다. 규조류중 띠돌말과에 속하는 Fragilaria capucina, Synedra acus, S. familiaris 가 우점종이였다. Environmental factors and microflora of Samchun-impoundment in Kyongsan, Kyongpook were studied. Average data of environmental factors analysis are as followings : air temperature 25.8℃, pH 6.77, total hardness 117.94mg/ℓ, SS 4.5mg/ℓ, DO 3.3mg/ℓ, BOD 6.6mg/ℓ, COD 7.2mg/ℓ, ?? 17.56mg/ℓ, ?? 7.14mg/ℓ, Mn 0.05mg/ℓ, Zn 0.04mg/ℓ. Total bacteria in 4 site were 3.32×?? No. inds/㎖. Distribution and species of phytoplankton and zooplankton were 86 species including 33 species of bacillariophyceae, 26 of chlorophyta, 8 of cyanophyceae, 13 of euglenaceae, 6 of dinophyceae. Fragilaria capucina, Synedra acus, S. familiaris were dominant species.

재원기간중 사망한 급성 심근경색증 환자의 임상적 특징

현대우,김기식,신이철,박소영,배장호,한창엽,김윤년,김권배 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.9

우리나라 사망구조의 지역별 차이에 관한 연구

한소현,이시백 서울大學校 保健大學院 2001 보건학논집 Vol.38 No.1

It is recognized that decreased mortality increasing the length of human life has been the major factor in acceleration of population increase. Therefore, death remains a subject of deep concern not only to the person but also government at all levels. The purpose of this present study is to measure the differences in death rates of region which persist the general reduction in mortality. The data for the present study of trend in differential mortality by region were derived from the official mortality statistic of Korea from 1980 to 1999. Mortality differentials were calculated for male and female by geographic region using age-adjusted death rates. It is apparent that there were considerable differences in age-adjusted death rates by region. Mortality was higher in Jeonam and Kyungbuk provinces for each sex than kyung-gi province and city area.

우리 나라 5대 사망원인의 사망구조에 관한 연구

한소현,이시백 서울대학교 보건대학원 2001 서울大學校 保健環境硏究所論叢 Vol.11 No.1

The purpose of present study is to investigate differential death rates of 5 leading causes of death. In this, therefore, analysis is made of age and regional differences in mortality rates by causes of death. The data for the present study were derived from the official mortality statistics of Korea from 1980 through 1999. The present study demonstrates that there are significant variation in mortality rates of 5 leading causes of death by region, sex, age and year observed. Death rates caused by circulatory disease and neoplasm are continuously increasing and high, while mortality by digestive system stay almost same level and relatively low. The geographical differences in mortality of 5 leading causes of death from neoplasm and circulatory system were higher in Jeonam and Kyungbuk and lower in city areas.

산업장 근로자를 대상으로 실시한 한방 건강검진의 만족도

한현정,정재열,권소희,손용선,장두섭,이기남 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.2

The examinees who received oriental and western health examination both and submitted questionnaire were 257 workers in workplace for 1 month during health examination for industrial workers. The research was to compare the satisfaction of oriental and western health examination by the questionnaire. The data that were collected by health examination were analyzed into frequencies, ANOVA, T-test with use of SPSS 10.0 program by the research purposes. The results were as follows: 1. Health examination in satisfaction comparison by the general characteristics of subject was significant in oriental health examination for the subjects who had 9-10 working hours(P〈0.05). 2. The workers who had high confidence in result of health examination, affirmative thinking for requirement of health examination, arbitrary decision for receiving the health examination, had high satisfaction in oriental and western health examination both, It was statistically significant difference. 3. For the difference in subjective health condition's recognition, the subject who answered " Healthy" had high satisfaction in western health examination, and it was statistically in western health examination, and it was statistically significant The subject who answered "Healthy" for the early detection of disease had high satisfaction in western health examination, The subject who answered skeptical for the early detection of disease had high satisfaction. in oriental health examination and it was statistically significant. 4. The respondent who said "Yes" for the question, whether you know about oriental health examination or not before receiving oriental health examinatgion had relatively high satisfaction For the question about including of oriental health examination continuously in the coming future, the subject who replied affirmative answer had high satisfaction in average, and it was significant difference(P〈0.05). 5. For the question that will be needed in the item of oriental health examination, the highest item was the requirement of specific oriental health examination for various diseases, followed by the requirement of medical specialist for individual need of medical examination by the item, and the prescription of herbal medicine, the medical examination of cancer, the interview for folk remedies, the parallel treatment for acupuncture cupping, etc, the medical examination for adult disease, the requirement of room for medical examination etc.

‘병동 중심의 처치수가 산정지침 개발’ 등을 통한 간호업무 표준화 및 그 효과

한혜정,설미진,김영주,박소영,박아리스,문한경,이현영 한국의료QA학회 2009 한국의료질향상학회지 Vol.15 No.1

문제: 병동 처치수가 산정 부정확 및 누락으로 인한 부적절한 재고관리로 진료차질, 불필요한 업무발생 및 수익이 감소한다. 목적: 병동 처치수가 산정 정확화를 위한 업무 표준화 도구 및 시스템을 개발하여 적정 재고관리를 통한 수익증대 및 직무만족도를 향상시킨다. 의료기관: 서울시 종로구에 소재한 대학병원 질 향상 활동: 병동중심의 처치 산정지침 개발 및 수가물품의 적정재고 관리방안을 모색하였다. 개선효과: 병동중심의 처치수가 산정 지침서를 제작하여 업무표준화를 기하였음. 응급청구 품목 및 수량이 ’07년 대비 71%감소하고 타 병동 차용품목이 활동 전에 비해 61% 감소, 수량은 77% 감소함. 응급청구 총소요시간이 활동 전에 비해 ’07년 대비 77% 감소하고 타 병동 차용 총 소요시간이 61% 감소함. 전년 동기간 대비 수익이 4% 증가하였으며 또한 54병동과 보험 심사팀에서 직원들의 직무만족도가 향상되었다.

혐오자극과 모델링 및 언어적 지시가 발달지체 아동의 상동행동에 미치는 효과 : 사례연구

한현민,함소희,김윤선,지지영,홍희영 대진대학교 교육연구소 2000 교육연구 Vol.2 No.1

The experiment was undertaken to determine the combined effects of aversive, modeling, and verbal instruction on the self-stimulatory stereotyped benavior exhibited by a three-year two-month old with developmental delay, The behavioral interventions were consisted of lemon juice(aversive), behavioral rehearsal(moleling), verbal instruction(prompting), and defferential reinforcement of incompatible behavior (DRI), and continued over 73 sessions. The data gathered across multitreatments design (A-BCD-A-BD-DE) were visually analyzed. The results revealed that all the treatments reduced the occurrences of stereotyped behavior, the significant differences among any interventions were not found, and more importantly, the behavior effects were maintained.

김치저장성 향상을 위한 동결방법이 배추조직의 물성변화에 미치는 영향

양진한,박소희,유진현,임호수,조재선,황성연 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.2

김치를 10˚C에서 8일간 숙성시켜 산도 0.6~0.7%로 숙성된 김치를 -70˚C와 -20˚C로 냉동하며 -20˚C에서 저장하면서 배추 조직의 elasticity, hardness, 세포 조직의 변화, 드립양을 실험한 결과는 다음과 같다. -70˚C에서 급속 동결한 것과 -20˚C에서 완만 동결한 냉동 김치 중 배추조직의 elasticity는 냉동 저장 15일까지 감소하다 일정하게 유지되었고 hardness는 거의 변화가 없었으며 냉동 방법에 따른 변화도 거의 나타나지 않았다. 투과 전자현미경으로 관찰한 결과 control의 경우 세포벽이 매우 두꺼우며 세포의 모양들이 잘 보존되어 잇는 것을 볼 수 있었고 -20˚C로 냉동 처리하여 해동시킨 세포벽들은 많이 손상되어 있음을 볼 수 있었으며 -70˚C로 급속 냉동시료의 경우 세포벽의 손상 정도가 -20˚C로 냉동 처리한 시료보다 덜 파괴되어 있음을 볼 수 있었다.한편 냉동 저장기간 동안 드립의 손실량의 변화는 -70˚C로 냉동 처리한 시료의 드립양은 3~4%정도로 -20˚C로 냉동 처리한 시료의 5~6%에 비해 적은 것을 알 수 있었다. This study was conducted to investigate the effect of freezing methods, show freezing at -20℃ and rapid freezing at -70℃, on physical properties of Chinese cabbage in frozen Kimchi during storage at -20℃. Elasticity of midrib of Chinese cabbage in frozen Kimchi was decreased until 15 days and did not changed thereafter during storage at -20℃, Hardness of that was not changed during storage. Those results of elasticity and hardness of slow frozen sample are similar to rapid frozen sample. By the morphological observation through transmission electron microscope, more of cellular structure of Chinese cabbage in slow frozen was destructed than that of rapid frozen sample. Drip loss was more in slow frozen sample than that in rapid one.