Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 ^(99m)Tc-Lactosylated Serum Albumin을 이용한 간 기능의 평가

정신영,이재태,서명랑,유정아,배진호,안병철,황재석,정재민,하정희,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

목적: ^(99m)Tc-lactosylated serum albumin (^(99m)Tc-LSA)은 간세포에 특이적으로 결합하는 간수용체 영상용 방사성의약품으로 새로이 합성되었다. 간섬유화를 유발하는 dimethylnitrosamine (DMN)을 투여한 간 손상 휜쥐 모델에서 ^(99m)Tc-LSa의 역동학적인 간섭취를 조사하고 간효소치의 변화와 조직학적 소견을 비교하여, LSA의 간섭취가 간기능의 변화를 반영하는지를 연구하였다. 대상 및 방법: SD계 흰쥐에 DMN를 27 mg/kg으로 복강 내 주사하여 급성 간손상을 유도하고 대조군과 비교하였다. DMN을 주사한 흰쥐를 3일(DMN-3), 8일(DMN-8), 21일(DMN-21)에 ^(99m)Tc=LSA (1,665 mg/kg) 29 MBq를 정맥 주사하여, 30분 동안 동적 영상을 획득하고 간과 신장부위에 관심영역을 설정하여 간과 심장부위의 시간방사능 곡선을 얻었다. 간기능 평가를 위해 시간방사능 곡선을 이용하여 간섭취지수와 혈중제거지수를 구하였고 곡선 최적화를 시행하였다. DMN 투여군과 대조군의 간효소치의 변화와 간조직의 광학현미경 소견을 비교하였다. 결과: 대조군에서는 ^(99m)Tc-LSA가 빠르게 간에 섭취되고 혈중에서 제거되었으나 DMN을 처리한 군에서는 간섭취가 낮았다. 간섭취지수의 비교에서 대조군에 비해 DMN 처리군에서 유의하게 간섭취지수가 낮았다(DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, 대조군: 0.96, p<0.05). 혈중제거지수의 비교에서도 대조군에 비해 DMN 처리군에서 혈중제거지수가 유의하게 높았다(DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, 대조군: 0.473, p<0.05). 비선형 회귀분석에서 R_(2) 값은 0.9이상으로 좋은 일치를 보였고, 대조군에ㅓ K값이 DMN처리군에 비해 크고(DMN-3: 0.28, DMN-8: 0.41, DMN-21: 0.46, 대조군: 0.97, p<0.05), T_(1/2)값은 작았다(DMN-3: 2.5, DMN-8: 1.7, DMN-21: 1.5, 대조군: 0.7, p<0.05). 간효소치의 변화는 DMN-3군에서는 대조군에 비해 상승하였으나 DMN-8, DMN-21군에서는 간효소치의 상승이 관찰되지 않았다. 간조직 소견의 경우 DMN-3군에서 중심정맥 주위에 괴사가 관찰되었으나 DMN-8군, DMN-21군에서는 미약한 정도의 염증세포 침윤만이 관찰되었다. 결론: ^(99m)Tc-LSA 간신티그래피의 간섭취 정도는 간손상과 반비례하였으며 간섭취의 변화는 조직학적 손상이 심한 정도와 간손상후 회복되는 과정을 반영하여 주었다. ^(99m)Tc-LSA 간신티그래피가 간손상을 평가하고 간손상후 회복되는 과정을 추적하는 간수용체 영상용 방사성 의약품으로 사용될 수 있을 것으로 생각된다. Objects: ^(99m)Tc-lactosylated human serum albumin(LSA) is a newly synthesized radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by dimethylnitrosamine(DMN) and results were compared with corresponding findings of liver enzyme profile and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for 30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program. Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat, compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat group than control rat group (DMN-3:0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control: 0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group. Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver transaminase and degree of histologic liver injury in rat with acute hepatic injury.

Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

컴포넌트 기반의 건강관리 시스템 개발을 위한 UI 툴

정호진;주문일;박광호;박태향;김희철 인제대학교 2012 仁濟論叢 Vol.27 No.1

Component-based systems are generally known as useful for maintenance and extension. However, there are a number of difficult aspects in designing them, and skillful knowledge of component-based design is strongly requested. Against the problem, this paper presents a User Interface tool that helps to develop component-based healthcare systems more easily and effectively. This UI tool has been developed, aiming at enabling easier and faster development of such systems. Further it supports standardization and interoperability. We expect that use of the UI tool helps to add, delete, change medical contents, and maintain related systems with less effort and better performance.

사회적 음주자에서 매운 맛 선호도가 급성 음주 반응에 대한 Naltrexone 효과에 미치는 영향

정희정,김성곤,김종현,박숙현,강철중 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.6

Capsaicin과 알코올이 각각 중추신경 아편계 활성도를 증가시킨다는 선행 연구들이 있다. 이에 본 연구는 매운맛 선호도에 따라 급성 음주 반응에 차이가 있는지와 급성 음주 반응에 naltrexone이 미치는 영향에 차이가 있는지를 조사하고자 하였다. 12명의 의과대학 남학생을 대상으로 두 군으로 나눈 후 cross-over design을 사용하여, 한 군(6명)은 naltrexone 25 mg을 실험 1일째에, 50 mg을 실험 2일러에 주었고, 다른 군(6명)은 naltrexone 25 mg을 실험 8일째에, 50mg을 실험 9일째에 주었다. 실험 2일째와 9일째에 각대상자의 Biphasic Alcohol Effects Scale (BAES)로 측정한 급성 음주 반응과 음주갈망, 혈중알코을 농도 등을 음주 전, 음주(0.6 ml/kg) 후 15분, 30분, 60분에 측정하였다. 통계 분석 시 Food Preference Scale 점수에 따라 모든 대상자를 매운 맛을 선호하는 군(strong preference for hot food, SP)과 매운 맛을 덜 선호하는 군(lesser preference for hot food, LP)의 두 군으로 다시 나누어 분석하였다. 결과는 다음과 같다. 1) Naltrexone을 투여 여부에 관계없이 알코올의 급성 흥분 효과는 매운 맛 선호도에 따른 차이가 없었다. 그리고 LP에서도 naltrexone의 투여 여부에 따른 알코올의 급성 흥분 효과의 차이가 없었다. 그러나 SP에서는 알코올의 급성 흥분 효과가 naltrexone 투여 시 유의하게 억제되었다. 2) 알코올의 급성 진정 효과, 음주 갈망 및 혈중 알코올 농도는 매운 맛 선호도 혹은 naltrexone 투여 여부에 따른 차이가 없었다. 본 연구에서 매운맛을 선호하는 경우 알코올의 급성 흥분효과가 naltrexone에 의해 억제되었다. 이것으로 naltrexone이 매운맛을 선호하는 군에서 알코을 의존 재발 예방 효과가 더 클 가능성을 제시한다. Objectives : Both capsaicin, a pungent substance of hot food, and alcohol, are known to affect central opioid activity. The purpose ofthis study was to investigate the difference in the subjective acute responses to alcohol and the effect of naltrexone on them among those who prefer hot food to varying degrees. Methods : Twelve male medical students were divided into two groups using a cross-over design. One group was given naltrexone on only the first (25 mg) and the second day (50 mg), and the other group was given naltrexone on only the eighth (25 mg) and the ninth day (50 mg). On the second and the ninth day, the acute effect of alcohol was assessed in all subjects, using the Biphasic Alcohol Effects Scale (BAES) just before drinking and at 15 minutes, 30 minutes and 60 minutes after drinking (0.6 ml/kg). Alcohol craving was also measured, using Visual Analogue Scale for craving (VAS-C) and blood alcohol concentration (BAC), at the same interval. For statistical analysis, subjects of both group were re-divided into two group, those with a strong preference and those with a less preference (LP) for hot (spicy) food (SP), using the Food Preference Scale. Results : 1) Repeated measures of ANOVA (2 preference groupsX4 time blocks) on the stimulative subscale of BAES yielded no significant group by block interaction of naltrexone administration. Repeated measures of ANOVA (2 drug groupsX4 time blocks) on the stimulative subscale of BAES revealed a significant group by block interaction in SP (p=0.028), but not in LP. The paired t-test revealed that significant suppression of the stimulative subscale of BAES was observed at 15 minutes and 30 minutes after drinking when naltrexone condition was compared with no-medication condition in SP(p=0.014 ; p=0.007). 2) For the sedative subscale ofBAES, VAS-C and BAC, repeated measures of ANOVA yielded nosignificant group by block interaction by either hot food preference or naltrexone administration. Conclusion : For those who prefer hot food, the effect of stimulative acute alcohol was suppressed by naltrexone. This result strongly suggests that naltrexone could prevent relapse more effectively in those who prefer hot food.

전유전체 서열 분석과 가시화를 위한 워크벤치 개발과 응용

최정현,진희정,김철민,장철훈,조환규 한국유전학회 2002 Genes & Genomics Vol.24 No.2

An increasing number of genome sequencing projects results in explosive growth of whole genome sequences. Furthermore the number of studies on the functions of individual genes has also been rapidly increased. However on-memory algorithms are not applicable to the analysis of whole genome sequences, since the size of individual whole gene a ranges from several million base pairs to hundreds billion base pairs. In order to effectively manipulate the huge sequence data, it is necessary to use the indexed data structure for external memory. In this paper, we introduce the development and application of the workbench for the analysis and visualization of whole genome sequences using string B-tree that is suitable for the analysis of huge data. This system consists of two main parts, the analysis query part and the visualization part. The query system supports various transactions such as pattern matching, k-occurrence, and k-mer analysis. The visualization system helps biologists to easily understand whole genome structure and specificity by various kinds of visualization such as whole genome sequence viewer, annotation viewer, CGR (Chaos Game Representation) viewer, k-mer viewer, RWP (Random Walk Plot) viewer, and map viewer. We can find the relationships among organisms, support gene prediction in a genome, and study the function of junk DNA using our workbench. In this paper, we apply our workbench to investigating specific sequence such as avoided sequence, common sequence, and classifiable sequence.

疲勞荷重을 받는 鐵筋콘크리트 보의 炭素纖維시트 補强에 따른 構造特性에 관한 硏究

조철희,김하진,김광섭,김정섭 朝鮮大學校 建設技術硏究所 1999 建設技術硏究 Vol.19 No.1

This study is an experimental study of reinforced-concrete beam with carbon fiber sheet under fatigue load and aims at utilizing it as the basic material of strengthening design of reinforced-concrete structure under the fatigue load by examining the structural characteristics according to the fatigue load. Non-strengthening and strengthening, kind of strengthening materials(non-resin carbon fiber sheet, resin carbon fiber sheet) and the loading size (80%, 100%, 120% of static destruction load) in static and fatigue experiment are designed as the variables of experiment. The experimental results are as follows. 1. The strengthening test specimen shows the increase of yield strength of 208% in comparison with non-strengthening test specimen in the static experiment which is the standard of fatigue experiment. 2. The strengthening test specimen of non-resin CFS of K company and of resin CFS of M company indicate the increase of yield strength of 228% and 183% respectively in static experiment. 3. It is found that the strengthening test specimen in fatigue experiment has the increase of fatigue strength according to that of static yield strength. The strengthening test specimen with the loading of 120% of static load secures the fatigue yield strength of greater cycle than non-strengthening test specimen with 80% of static destruction load. Accordingly, it is judged that it is suitable to use CFS for the strengthening of reinforced-concrete due to the lack of fatigue load and structural yield strength. 4. It is shown that non-resin CFS strengthening test specimen of K company tested under the same condition secures the fatigue yield strength of greater cycle than resin CFS strengthening test specimen of M company. Initial deflection and elastic behavior have a similar form. Therefore it is thought that non-resin CFS produced at home is the strengthening material with the quality of international standard. 5. The test specimen reinforced with CFS has greater effects at static and fatigue yield strength, prevention of initial deflection and elastic deflection. So, it is considered that the reinforcement of CFS structure in actual structure may have the similar reinforcement effect to this experiment

Drosera rotundifolia와 D. spatulata의 조직배양시 식물체 재생에 미치는 몇가지 영향

권수정,김학현,이철희 충북대학교 첨단원예기술개발연구센터 2000 연구보고서 Vol.5 No.-

Drosera spatulata는 영양분의 요구도가 낮아 1/2MS배지에서 가장 많은 식물체가 재생되었다. 대부분의 Drosera속 식물들은 식물체의 재생과 생장에 질소원의 요구도가 낮아 D. spatulata와 D. rotundifolia의 경우 MS배지의 1/4배에서 가장 많은 식물체가 재생되었다. 적정 sucrose의 농도는 식물의 종마다 달라 D. spatulata의 경우 4%에서 D. rotundifolia는 1%에서 무처리구에 비해 많은 식물체의 재생을 보였다. 대부분 활성탄은 식물체의 재생에 효과적이었으나 뿌리의 형성은 억제되는 경향이었다. D. spatulata는 0.05%에서, D. rotundifolia는 0.01%에서 가장 많은 식물체 재생을 보였다. 식물체 재생은 D. spatulata는 0.6%의 agar를 첨가한 배지에서 D. rotundifolia는 액체배지에서 가장 효과적이었다. pH 실험의 결과 D. spatulata는 pH 4.5에서 가장 많은 식물체 재생을 보였다. The study was conducted to fine out the effect of Drosera species showed generally low nutrient requirement, the highest shoot production was obtained with 1/2 strength of MS medium for D. spatulata than with any other strength of MS medium tested. The nitrogen requirement of Drosera species were also relatively low. The maximum shoot regeneration was achieved when cultured in 1/4 strength of nitrogen contained MS medium. The optimum sucrose concentration for each species was different, such as 4% for D. spatulata; 1% for D. rotundifolia. In general, activated charcoal had positive effect on shoot proliferation, but had negative effect on root formation of Drosera species. The best shoot regeneration was achieved on MS medium containing 0.05% activated charcoal for D. spatulata, 0.01% for D. rotundifolia. Shoot regeneration of Drosera rotundifolia was better in MS liquid medium and D. spatulata showing best results on 0.6% agar. The maximal shoot proliferation was achieved on the media adjusted to pH 4.5 for D. spatulata respectively.

제1회 한국물리탐구토론대회에 출제된 문제 풀이에 대한 분석 : 『Adhesive tape』와 『Fractal Diffraction』을 중심으로 centering around problems on "adhesive tape" and "fractai diffraction"

육근철,김용복,이희복,정기주,김준태 공주대학교 과학교육연구소 2003 과학교육연구 Vol.34 No.-

제1회 한국물리탐구토론대회에는 10개의 문제가 출제되었는데 본 연구에서는 이들 10개의 문제 중 『접착테이프(Adhesive tape)』 문제를 해결한 5개 팀의 보고서와 『Fractal Diffraction』 문제들 해결한 고등학생부 3개 팀과 중학생부 1개 팀의 보고서를 비교 분석하였다. 그 결과 학생들은 접착테이프를 갑자기 뗄 때 빛이 발생하는 원인을 정전기 발생, 전자의 이동, 에너지의 변환 등으로 해석하였다. 그리고 Fractal 문제의 경우 학생들은 Fractal 이라는 개념을 제대로 이해하지 못하여 잘못된 방향으로 문제를 해결한 팀이 많았다. 특히 『Fractal Diffraction』문제는 출제된 10문제 중 가장 까다로운 문제로 학생들이 생각하고 있는 것으로 조사되었다. 본 대회는 탐구 토론대회이므로 탐구의 과정별로 새로운 아이디어를 제안하여 결론에 이르는 창의적 탐구 과정이 제안되어야 한다. We have analyzed students' reports on two problems such as "adhesive tape" and "fractal diffraction" among ten assigned-problems in the first Korean youth physics tournament. (KYPT). There were five team's reports on "adhesive tape" and four team's reports on "fractal diffraction". Students analyzed that the reason for lightening coming out from the stripping down adhesive tape ma be caused by electrostatic friction, electron transfer, energy transformation, etc. However, many students failed to solve the "fractal diffraction" problem correctly. This pro blem is found to be most difficult one among ten problems in the first KYPT for the reports should include the creative investigations by their own ideas in every steps of inquiries.

개에서 각막궤영에 대한 치료 1례

박희서,정성목,박창식,김명철 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

A five years old female Shih-tzu was presented to the Veterinary Mediccal Teaching Hospital of Chungnam National University. The dog had been treated corneal damage on left eye in the local animal hospital for 2 months caused by trauma. Conjunctival congestion. corneal edema. blepharospasm, photophobia, epiphora. purullent discharge were observed. In Schirmer tear test. the result was within normal range, in slit lamp examination there was no indication of any abnormality except the corneal region. Corneal epithelium was stained by fluorescein dye and antibiotic susceptibility test was performed. For treatment, antibiotics and mucolytic agent, NSAIDs and atropine were topically administered for two weeks. As a result, non-healing corneal ulceration had finally disappeared when rechecked after only topical treatment.

관상동맥 스텐트 시술 후의 재협착에 관한 연구

김윤철,이정우,김보영,강정아,임대승,이민수,김정희,성보영,최성준,성인환,전은석 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.1

Coronary stent implacement is known as an effective treatment in the intimal dissection after percutaneous transluminal coronary angioplasty and the prevention of restenosis. However, In-stent restenosis still remains a major concern in clinical stenting. The stents were placed in 103 patients from July 1996 to March 1999 and performed follow-up coronary angiograms in 59(57.3%) patients. To identify the clinical, angiographic and procedurerelated variables 'which predict late restenosis within the stented artery, 59 patients(58.3±9.9, M:F= 41:18) were studied. The clinical characteristics of the patients were stable angina in 23(39.0%), unstable angina in 14(23.7%), acute myocardial infarction in 21(35.6%) and old myocardial infarction in 1(1.7%). Coronary stenting was performed in 1 patient(1.7%) for primary lesion, 50 patients(84.7%) for suboptimal results after PTCA, 6 patients(10.2%) for bail-out procedure, and 2 patients(3.4%) for restenotic lesions. All patients were treated with aspirin and ticlopidinc. The follow-up angiograms were obtained at 7±4 months. The overall in-stent restenosis rate was 27.1%. The coronary angiographic findings were 32 single vessel(54.2%), 19 two vessel(32.2%) and 8 three vessel disease(13.6%). The angiographic morphological characteristics were type A in 33(55.9%), type B in 14(23.7%), type C in 12(20. 3%) cases. Variables of 16 patients with restenosis were compared with those of 43 patients without restenosis. Previously known predictors for in-stent restenosis were multiple stenting, stenting for restenotic lesions, residual stenosis after stenting, stenting for total occlusion lesions, reference diameter, balloon to vessel ratio, acute gain and minimal luminal diameter after procedure, design and characteristics of stents, ostial lesion of aorta, high pressure method for stenting, lesion length, diabetes mellitus, size of artheroma, saphenous vein grafts, ulcerlating lesions and calcified lesions. In this study, Reference diameter before stenting(2.43±0.54mm vs. 2.88±0.65mm, p=0.016) and balloon-to-artery ratio(1.28±0.26 vs. 1.11±0.18, p=0.006) were predictors for in-stent restenosis. 1) The overall in-stent restenosis rate was 27.1%. 2) In the analysis of predictors for in-stent restenosis, there was no significant differences in clinical, angiographic factors between group with restenosis and without restenosis. But, Only reference diameter before stenting and balloon-toartery ratio were predictors of late in-stent restenosis. In conclusion, stenting is effective revascularisation method for selected patients with ischemic heart disease, and to minimize in-stent restenosis rate, stent implanting is achieved in a large vessel on the basis of an artery-to-stnet ration of 1:1, if possible.