A selective cyclin-dependent kinase 4, 6 dual inhibitor, Ribociclib (LEE011) inhibits cell proliferation and induces apoptosis in aggressive thyroid cancer

Lee, Hyun Joo,Lee, Woo Kyung,Kang, Chan Woo,Ku, Cheol Ryong,Cho, Yoon Hee,Lee, Eun Jig Elsevier 2018 Cancer letters Vol.417 No.-

<P><B>Abstract</B></P> <P>The RB-E2F1 pathway is an important mechanism of cell-cycle control, and deregulation of this pathway is one of the key factors contributing to tumorigenesis. Cyclin-dependent kinases (CDKs) and Cyclin D have been known to increase in aggressive thyroid cancer. However, there has been no study to investigate effects of a selective CDK 4/6 inhibitor, Ribociclib (LEE011), in thyroid cancer. Performing Western blotting, we found that RB phosphorylation and the expression of Cyclin D are significantly higher in papillary thyroid cancer (PTC) cell lines as well as anaplastic thyroid cancer (ATC) cell lines, compared with normal thyroid cell line and follicular thyroid cancer cell line. LEE011 dose-dependently inhibited RB phosphorylation and also decreased the expressions of its target genes such as <I>FOXM1, Cyclin A1,</I> and <I>Myc</I> in ATC. Furthermore, LEE011 induced cell cycle arrest in G0-G1 phase and cell apoptosis, and inhibited cell proliferation in ATC. Consistently, oral administration of LEE011 to ATC xenograft models strongly inhibited tumor growth with decreased expressions of pRB, pAKT and Ki-67, and also significantly increased tumor cell apoptosis. Taken together, our data support the rationale for clinical development of the CDK4/6 inhibitor as a therapy for patients with aggressive thyroid cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> pRB and Cyclin D were expressed high in aggressive thyroid cancer. </LI> <LI> LEE011 suppressed pRB and also decreased the expressions of its target genes in ATC. </LI> <LI> LEE011 induced cell cycle G1 arrest and apoptosis, and inhibited cell proliferation. </LI> <LI> LEE011 inhibited in vivo tumor growth with decreased expressions of pRB and Ki-67. </LI> <LI> We could explain the anticancer effects with the RB-E2F pathway. </LI> </UL> </P>

김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

벼, 콩 및 채소류에 대한 Mn-54, Co-60, Zn-65, Cs-137의 토양-작물체간 전이계수

박찬걸,조용우,최용호,김국찬,이창우,이강석,이정호 대한방사선 방어학회 1991 방사선방어학회지 Vol.16 No.2

벼, 콩, 상치, 당근, 호박의 가식부위에 대하여 몇가지 토양에 있어서 토양-작물체간 Mn-54, Co-60, Zn-65, Cs-137의 전이계수를 포트 재배에 의한 방사성 추적자 흡수실험을 통하여 조사하였다. 핵종간 전이계수는 거의 모든 경우 Zn-65>Mn-54>Cs-137>Co-60의 순이었다. 콩이 벼보다 전반적으로 한 자리 정도 높은 값을 보였고 채소류의 경우에는 대체로 상치에서 가장 높고 호박에서 가장 낮은 값을 보였다. 강산성 토양에서는 약산성 토양에서보다 전이계수가 훨씬 높았다. 본 조사결과에 입각하여 한국인의 섭식경로 피폭선량 평가에 이용하기 위한 각 핵종의 전이계수치가 작물별로 제안되었다. Soil-to plant transfer coefficients of Mn-54, Co-60, Zn-65 and Cs-137 were estimated for the edible parts of the rice, soybean, lettuce, carrot, and squash grown in different soils by radiotracer uptake experiments using pot cultures. The transfer coefficients of radionuclides were n the order of Zn-65>Mn-54>Cs-137>Co-60 in most of the cases studied. The coefficients for soybean were roughly and order of magnitude higher than those for rice. Among vegetables, lettuce mostly showed the highest value and squash, the lowest. In the strongly acidic soils. From the data obtained, crop-specific transfer coefficients of the four nuclides were proposed for the use in Korean food-radiation dose assessment.

골다공성 척추 압박골절에서 경피적 척추성형술 후 강화된 척추체에 의한 합병증 : Hammer Effect Preliminary Report

전은오,이상구,유찬종,김우경,박찬우,이언 The Korean Neurosurgical Society 2003 Journal of Korean neurosurgical society Vol.34 No.6

부신피질 자극호르몬 단독결핍 1례

박경식,이승현,최석영,박태규,이동화,이찬우,정성복,이인규 啓明大學校 醫科大學 1997 계명의대학술지 Vol.16 No.3

부신피질 자극호르몬 단독결핍은 국내에서 7례만이 보고된 희귀 질환이며 병인 및 경과 등 아직 불분명한 측면이 많다. 이는 질환지체의 희귀성도 있지만 치료후 자연경과에 대한 장기적인 추적검사가 이루어진 경우가 드물기 때문이기도 하다. 저자들은 비특이적인 전신증상을 주소로 내원한 폐경기후의 56세 여자 환자에서 자가면역질환의 증거없이 고프로릭틴혈증을 보인 부신피질 자극호르몬 단독결핍 1례를 경험하고 1년간의 부신피질호르몬 대치요법후 추적검사를 실시하였기에 문헌 고찰과 함께 보고하는 바이다. Isolated ACTH deficiency is a rare disorder which shows secondary adrenal insufficiency with normal states of other pituitary hormones. A 56-year-old postmenopausal woman complaining general weakness and intermittent mental confusion presented negligible serum cortisol and ACTH level with hyperprolactinemia and mild hyponatremia. Rapid ACTH stimulation test did not show significant increase of serum cortisol level. Combined pituitary stimulation test showed negligible response of cortisol and ACTH, and slightly decreased response of prolactin and gonadotropin. But the responses of TSH and GH were normal. There was no evidence of organic pituitary of hypothalamic lesion in brain MRI and antithyroid autoantibody was not detected in serum. After receiving 12 months of glucocorticoid replacement therapy, she skipped the medicine for 3 weeks and we performed follow-up endocrinologic tests. Serum ACTH and cortisol level was also negligible and prolonged ACTH simulation test showed normal response of serum cortisol. CRF stimulation test showed no response of serum ACTH. Basal prolactin level was decreased to near normal and combined pituitary stimulation test showed normal response of prolactin with slightly blunted response of TSH and GH. We concluded that the case was nonautoimmune origined isolated ACTH deficiency which was accompanied by hyperprolactinemia and resultant deficiency of gonadotropin, so we report this case with review of literatures.

Zn₄SnSe_(6):Co^(2+) 단결정의 물성에 관한 연구

이우선,오금곤,최창주,송찬일,김형곤 조선대학교 에너지.자원신기술연구소 2001 에너지·자원신기술연구소 논문지 Vol.23 No.2

In this paper, author describe the undoped and Co^(2+)-doped Zn₄SnSe_(6) single crystals were grown by the chemical transport reaction(CTR) method. For the crystal growth, the temperature gradient of the CTR furnace was kep a t 700℃ for the source aone and at 820℃ for the growth zone for 7-days. I t was found from the analysis of x-ray diffraction that undoped and Co^(2+)-doped Zn₄SnSe_(6) compounds have a monoclinic structure. The optical absorption spectra obtained near the fundamental absorption edge showed that these compounds have a direct energy gaps.

한민족 화합을 위한 남북체육교류 활성화 방안에 관한 연구

이정흔,우찬명,김홍설 忠南大學校體育科學硏究所 1995 體育科學硏究誌 Vol.13 No.1

The purpose of this research is to propose an approach to creating an atmosphere in which peace and reunification on the Korean peninsula can be achieved through north-south sport exchanges and cooperation. This proposal is intended to show how cooperative north-south sports exchanges will foster an atmosphere conductive to peaceful unification by recovering the homogeneity of the Korean people. The main characteristics of this study are as follows: First, future north-south sports exchanges should not be managed by government agencies, but will require the formation of specialized civilian organization which can carry out the necessary operations. Second, advancement of the north-south sport exchanges will require the establishment of a "North-South Sport Information Reference Center". Third, a north-south cultural facility which would include a unified sport center(including an athlete's village)must be established. Fourth, north-south sports exchanges and cooperation will require the establishment of systematic provisions for the financial support required to advance this endeavor. Fifth, in order to activate the commercial support needed for these exchanges, recognition of the importance of the endeavor will be called for from all sports minded people.

사출성형제품의 런너시스템 설계 지식베이스화에 관한 연구

이찬우,허용정 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

본 논문은 사출성형제품의 런너시스템 설계를 합리적으로 수행하기 위해 사출금형설계전문가의 축적된 지식과 경험을 발췌 정리하여 지식베이스를 작성하였으며, 설계에 필요한 제품의 형상정보를 제공하기 위해 총칭형상과 특징형상 개념을 이용하여 특징형상을 정의하였다.또한 지식베이스를 통해 산출된 설계결과를 기하학적 모델러와의 인터페이스를 통해 3차원 형상으로 보여지도록 하였으며, 최종 생성된 3차원 기하학적 형상정보는 CAE 모듈이나 CAPP 모듈에서의 후속작업을 위해 제공될 수 있도록 구축되었다.

사출금형설계를 위한 전문가시스템에 관한 연구

이찬우,허용정 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

본 논문은 사출금형설계 관련 지식과 경험을 전산정보화 하고 유동해석프로그램과 기계적 성능예측 프로그램을 결합시켜 전문가시스템을 구축하였다.전문가시스템은 주어진 설계에 대해 유동해석프로그램을 구동시켜 열기계적 데이터베이스를 얻고, 필요에 따라 기계적 성능예측 프로그램을 수행시켜 설계의 최종적인 진단을 수행하게 된다.이러한 과정으로 설계에 대한 평가를 수행하며, 만일 설계가 잘못된 것으로 판정이 되면 그에 해당하는 대안을 제시토록하여 합리적인 설계가 가능하도록 하였다.

철강용 알칼리탈지제의 제조와 그의 특성화

이찬우,편무실,공유식,박홍수 明知大學校 産業技術硏究所 1993 産業技術硏究所論文集 Vol.12 No.-

Alkaline degreasing agent(ADA) was prepared by blending of POE(6)-2-ethylhexyl ether(PEH-6). Tetronix T-701(T-701). Na2CO3, tetrasodium pyrophosphate(TSPP). Demol N, and MJU-100A. The physical properties of ADA tested with steel specimen showed the following results. The degreasing powers of ADA-6(PEH-6 20g/Na2CO3 40g/TSPP 20g/Demol N 5g/MJU-100A 5g/water 100g mixture) for press-rust preventing oil were 97% and 98% degreasing at 4wt%, 70℃ and 90℃respectively: for quenching oil, the degreasing power of ADA-6 was 94% degreasing at 4wt% and 70℃. From these results, it was confirmed that ADA-6 exhibited a good degreasing power. Foam heights measured immediately after foaming by Rose & Miles method and Ross & Clark method at 6wt%, 60℃ were 20mm and 50mm. respectively. As the result, ADA-6 was proved a good low foaming degreasing agent.