Molecular characterization of highly pathogenic avian influenza H5N8 viruses isolated from Baikal teals found dead during a 2014 outbreak in Korea

김설희,Moonsuk Hur,서재화,우찬진,왕승준,박응로,황종경,안인정,조성덕,Jeong-Hwa Shin,Seung-Do Yu,최경희,이동훈,Chang-Seon Song 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.3

Nineteen highly pathogenic avian influenza (HPAI) H5N8 viruses were isolated from wild birds in the Donglim reservoir in Gochang, Jeonbuk province, Korea, which was first reported to be an outbreak site on January 17, 2014. Most genes from the nineteen viruses shared high nucleotide sequence identities (i.e., 99.7% to 100%). Phylogenetic analysis showed that these viruses were reassortants of the HPAI H5 subtype and the H4N2 strain and that their hemagglutinin clade was 2.3.4.4, which originated from Eastern China. The hemagglutinin protein contained Q222 and G224 at the receptor-binding site. Although the neuraminidase protein contained I314V and the matrix 2 protein contained an S31N substitution, other mutations resulting in oseltamivir and amantadine resistance were not detected. No substitutions associated with increased virulence and enhanced transmission in mammals were detected in the polymerase basic protein 2 (627E and 701D). Non-structural-1 was 237 amino acids long and had an ESEV motif with additional RGNKMAD amino acids in the C terminal region. These viruses caused deaths in the Baikal teal, which was unusual, and outbreaks occurred at the same time in both poultry and wild birds. These data are helpful for epidemiological understanding of HPAI and the design of prevention strategies.

일부지역 노인의 구강건강영향지수 비교 연구

김설희 한국치위생학회 2009 한국치위생학회지 Vol.9 No.2

Analysis of Correlation among Oral Environment, Oral Myofunction, and Oral Microorganisms

김설희 한국치위생과학회 2019 치위생과학회지 Vol.19 No.2

Background: Factors affecting oral function include tooth number, oral muscle strength, and oral diseases. This study aimed to investigate the relationship among oral environment, muscle, and microbiology. Methods: Fifty-six elderly individuals in a day care center were included in the study. The survey regarding tongue and lip muscle strength and oral microorganisms was conducted from November to December 2018. Results: Tongue and lip muscle strength were greater in men than women (p>0.05). Tongue muscle strength was greater in the ≤80-year-old group (34.94±9.85) than the ≥90-year-old group (25.57±7.54) (p<0.05). Tongue muscle strength and lip muscle strength were greater in the ≥15 functional teeth group (34.08±9.31 and 9.25±1.63, respectively) than in the <15 functional teeth group (28.08±7.53 and 7.76±1.51, respectively) (p<0.05). Age was significantly correlated with functional tooth number, denture use, and tongue muscle strength. The number of functional teeth was positively correlated with tongue muscle strength, lip muscle strength, and oral microorganisms. Denture use was negatively correlated with tongue and lip muscle strength. Tongue muscle strength was significantly correlated with lip muscle strength. The number of Eubacterium nodatum was higher in men than women. The number of Parvimonas micra and Enterococcus faecalis was higher in the groups with ≥15 functional teeth, denture use, and greater tongue and lip muscle strength. The number of Lactobacillus casei was higher in the group that uses dentures and with greater tongue strength. Conclusion: Oral microbiology is more important in oral environment and management than oral muscle function. The correlation between oral muscle and oral microorganism requires further study. Therefore, oral care training should be conducted to improve the oral care practice of elderly individuals, maintain oral health through oral care, and prevent the decrease in saliva secretion by aging.

대학생의 자기효능감, 개인・집단주의 성향, 문화적 역량이 진로태도에 미치는 영향

김설희,정헌식 인문사회 21 2020 인문사회 21 Vol.11 No.5

This study is intended to understand the influence of self-efficacy, individualism and collectivism and cultural competence on career attitudes of university student. The results of a survey of 308 students from K university’s 4 majors are as follows. There was a significant difference in self-efficacy by gender, major and there were significant differences in individualism/ collectivism, cultural competence, and career attitude by major. Self-efficacy had a positive correlation with individualism/collectivism, cultural competence, and career attitude. As a result of the regression analysis on the factors influencing career attitude, self-efficacy (confidence, efficacy) and cultural competence (experience, knowledge) had a significant effect. Explanatory power was 54.2%. In order to support the career of college students, program development and counseling support were required that considers not only majors and academic achievements, but also self-efficacy, disposition, and cultural competence. 본 연구는 전공계열별 대학생의 자기효능감, 개인・집단주의 성향과 문화적 역량이 진로태도에 미치는 영향요인을 분석한 조사연구이다. K대학교 4계열 전공 308명의 학생을 대상으로 조사한 결과는 다음과 같다. 첫째, 성별, 전공별 자기효능감에 유의한 차이가 있었고, 전공별 개인・집단주의 성향, 문화적 역량, 진로태도에 유의한 차이가 있었다. 둘째, 자기효능감은 개인・집단주의, 문화적 역량, 진로태도와 정적 상관관계가 있었고, 진로태도는 개인・집단주의 성향, 문화적 역량과 정적 상관관계가 있었다. 셋째, 진로태도에 영향을 미치는 요인에 관한 회귀분석 결과 자기효능감(자신감, 효능감), 문화적 역량(경험, 지식)이 유의한 영향을 미쳤으며, 설명력은 54.2%였다. 대학생의 진로지원을 위해 전공, 학업성취도뿐만 아니라 자기효능감과 개인・집단주의 성향, 문화적 역량을 고려한 프로그램 개발 및 상담지원이 요구되었다.

Improved accuracy in periodontal pocket depth measurement using optical coherence tomography

김설희,강세룡,박희정,김준민,이원진,Tae-il Kim 대한치주과학회 2017 Journal of Periodontal & Implant Science Vol.47 No.1

Purpose: The purpose of this study was to examine whether periodontal pocket could be satisfactorily visualized by optical coherence tomography (OCT) and to suggest quantitative methods for measuring periodontal pocket depth. Methods: We acquired OCT images of periodontal pockets in a porcine model and determined the actual axial resolution for measuring the exact periodontal pocket depth using a calibration method. Quantitative measurements of periodontal pockets were performed by real axial resolution and compared with the results from manual periodontal probing. Results: The average periodontal pocket depth measured by OCT was 3.10±0.15 mm, 4.11±0.17 mm, 5.09±0.17 mm, and 6.05±0.21 mm for each periodontal pocket model, respectively. These values were similar to those obtained by manual periodontal probing. Conclusions: OCT was able to visualize periodontal pockets and show attachment loss. By calculating the calibration factor to determine the accurate axial resolution, quantitative standards for measuring periodontal pocket depth can be established regardless of the position of periodontal pocket in the OCT image.

협력학습을 적용한 치위생 교육 연구

김설희 대한치과위생학회 2013 대한치과위생학회지 Vol.15 No.4

The Effects of Orofacial Myofunctional Training on the Changes of Lip and Tongue Strength in Elderly People

김설희,Min-Ju Kim,Seung-Hyun Lee,Bo-Wha Choi,Yae-Jee Heo 한국치위생과학회 2019 치위생과학회지 Vol.19 No.4

Background: Increasing age and loss of teeth weaken oral muscle strength. This study aimed to investigate the positive effects of orofacial myofunctional training on elderly people. Methods: Thirty six elderly individuals in a welfare center and a senior citizen center were included in this study. A survey regarding lip and tongue strength suvsequent to orofacial myofunctional training and oral health-related quality of life was conducted from April to June, 2019. Data were analyzed using PASW statistics ver. 18.0. Results: The experimental group, showed an increase in lip strength (from 9.79 to 10.31) and tongue strength (from 41.26 to 43.97) after orofacial myofunctional training. The perception of oral health-relatedquality of life was enhanced (from 2.26 to 2.07). In particular, the frequency of dry cough for food removal while swallowing decresed due to an incresed in the secretion of saliva (p<0.05). Correlation analysis showed that the number of functional teeth was positively related to oral health-related quality of life. Conclusion: Orofacial myofunctional training for improving lip and tongue strength is effective in the elderly. In older societies, oral strength should be maintained to improve oral health-related quality of life. It also suggests the interest of oral health workers and applying orofacial myofunctional training programs.

토요일의 귀가소동

김설희 샘터사 1992 월간 샘터 Vol.23 No.10

동일 환자에서 분리한 Mycobacterium Avium Complex (MAC) 균주들의 Clarithromycin의 상이한 Minimal Inhibitory Concentration에 대한 원인 파악

김설희,남소단,이지현,유희경,고원중,심명섭,강희윤,최성원,김창기,박영길,김희진,이승헌 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.0

목적: 결핵연구원으로 약제감수성검사를 의뢰한 Mycobacterium avium complex (MAC) 균주들 중에서 2-4달 간격으로 동일환자에서 분리한 균주들에 대해서 clarithromycin의 minimal inhibitory concentration (MIC)가 상이하게 나타나는 경향이 있어 이에 대한 원인을 확인하고자 하였다. 성적: 비결핵항산균 약제감수성검사를 의뢰한 MAC 균주들 중에서 larithromycin의 MIC 결과가 내성→감수성 혹은 감수성→내성→감수성으로 나타낸 균주들을 선별하였다. 이들 균주들에 대한 stock을 이용하여 약제감수성검사를 재실시하여 이전 결과와 비교하였고, one colony를 분리하여 PCR-based restriction enzyme assay 법을 이용하여 동정하였다. 그리고, one colony에 대한 clarithromycin의 MIC를 확인하였다. 전체 14균주(7 cases, 2균주/case)의 stock에 대한 clarithromycin의 MIC 재시험에서는 1균주를 제외하고 이전 검사결과와 동일한 결과를 나타내었다. One colony를 분리한 결과에서는 6균주(1균주/case)에서 3일에 colony가 형성되었으며, 이들 colony를 대상으로 실시한 동정에서는 Mycobacterium abscessus 5균주, Mycobacterium fortuitum complex 1균주로 나타났으며, clarithromycin의 MIC 재시험한 결과에서는 M. fortuitum complex는 감수성, 모든 M. abscessus는 내성으로 나타났다. one colony로 분리하여 MAC으로 동정된 6균주(1균주/case)에 대해서는 이전 검사결과와 동일하게 나타났다. 결론: 동일 환자에서 분리한 균주들에 대해서 수차례 실시한 약제감수성검사의 결과가 상이하게 나타나는 경우가 생긴다면 MAC 외의 rapid growing mycobacteria 혼합에 대해 염두에 두어야 할 것으로 판단된다. 또한 검사기관에서도 검사결과의 재현성에 대해 신중을 기해야 할 것으로 판단된다.

사용자 맞춤 클립핑을 위한 크롬 확장 프로그램의 설계 및 구현

김설희,김연지,심재문,김현진,임병수,이상준 한국정보과학회 2015 데이타베이스 연구 Vol.31 No.2

사용자가 현재 방문하고 있는 웹 사이트를 다시 방문하기 위하여 브라우저에서 제공하는 ‘즐겨찾기’ 또는 ‘북마크’ 기능을 이용하여 사이트의 정보를 저장한다. 브라우저에서 제공하는 ‘즐겨찾기’ 또는 ‘북마크’는 해당사이트의 정보를 제목 한 줄로 표현하여 사용자가 얻은 정보를 기억하기 어렵고, 단순한 주소 저장 기능만을 제공하고 있다. 브라우저에서 제공하는 ‘즐겨찾기’ 또는 ‘북마크’의 단점을 극복하기 위하여, 본 논문에서는 Google Chrome 브라우저 환경에서 사용할 수 있는 사용자 맞춤 클립핑을 위한 확장프로그램을 제안한다. 제안된 프로그램은 웹 페이지의 텍스트 디자인을 직접 수정 할 수 있는 기능과 사용자들이 웹 페이지에 다양한 기록을 할 수 있는 메모기능을 제공하여 다양한 정보를 저장할 수 있다. In general, users save information of a site using browser functions such as 'favorite' and 'bookmark' to revisit the site. These functions describe the web site very simply, and it is not easy for the user to remember the information of the web site. To overcome the shortcomings of these functions, we propose an extension program for user-customized clipping with Google Chrome Browser. This program provides efficient facilities that enable users to modify text-design in web pages and memo functions that can write various contents on the web pages, so that users can save more information for web contents.