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Di-2-ethylhexyl phthalate 처리 남아프리카산발톱개구리에서의 vitellogenin 발현
박응로,이철우,류지성,남성숙,전성환,나진균,최덕일,박광식 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.1
The estrogenic potency of di-2-ethylhexyl phthalate (DEHP) using reverse transcriptase-PCR response of liver vitellogenin mRNA in male African clawed frog (Xenopus laevis) was studied. Male frogs were injected with DEHP at dose of 300 ㎍/kg and 300 mg/kg body weight through the dorsal lymph sac. After 4 days, using suitable pair of RT-PCR primers, vitellogenin mRNA induction in the liver was measured and DEHP showed vitellogenin mRNA induction in only the group treated with 300 mg/kg. Any significant histological abnormalities by the exposure of DEHP was not shown in both testis and liver.
최경민,박응로,주홍신,양재경,이기영,이성택,이무춘,Choi, Kyung-Min,Park, Eung-Roh,Ju, Hong-Shin,Yang, Jae-Kyung,Lee, Ki-Young,Lee, Sung-Taik,Lee, Mu-Choon 유기성자원학회 1996 유기물자원화 Vol.4 No.2
자연계로부터 3종의 광합성 세균 strain KN 1-1, KN 2-1과 KN 2-3을 분리하여 돈분 폐수 처리에 대한 연구를 수행하였다. 유기산이 첨가된 배지에서 광합성 세균의 생육은 유기산을 첨가하지 않은 경우에 비해 2~3배 증가하였으며, bacteriochlorophyll a 함량도 1.5~2배까지 증가함을 보였다. 또한 축산 농가에서 직접 채취한 돈분 폐수에 광합성 세균을 첨가하였을 때 COD 감소율은 KN 1-1인 경우 80%, KN 2-1 89%, KN 2-3 75%를 나타내었다. Photosynthetic bacteria, strains KN 1-1, KN 2-1 and KN 2-3 were isolated from nature, and were studied for swine wastewater treatment. Growth of those photosynthetic bacteria were increased to 2~3 fold in organic-acid added medium(sodium acetate 1g, sodium propionate 1g and sodium butyrate 1g in Lascelles basal medium $1{\ell}$) than cultivation in Lascelles basal medium, and amount of bacteriochlorophyll a were increased to 1.5~2 fold. Chemical Oxygen Demand(COD) in swine wastewater using photosynthetic bacteria, strains KN 1-1, KN 2-1 and KN 2-3 were reduced 80%, 89% and 75%, respectively.
최경민,양재경,박응로,배진우,서용기,이성택,Choi, Kyung-Min,Yang, Jae-Kyung,Park, Eung-Roh,Bae, Jin-Woo,Seo, Yong-Ki,Lee, Sung-Taik 유기성자원학회 1997 유기물자원화 Vol.5 No.1
5-Aminolevulinic acid (ALA) 생합성의 $C_5$ 경로의 전구물질인 L-glutamic acid가 Rhodospirillum rubrum N-1 새포내에서 ALA 생산의 역할을 검토하였다. Lascelles의 기본배지에 L-glutamic acid와 levulinic acid (LA)를 각각 30, 20 mM 첨가배양으로써 균체외 ALA 생산성이 40배 증가(76 mg/l)하였다. 한편 $C_4$ 경로의 기질인 glycine과 succinic acid를 대수기 중기에 각각 60 mM 첨가함으로써, 균의 증식은 억제되었으나 균체외의 ALA는 52 mg/l에 달하였다. The role of L-glutamic acid, a precursor of $C_5$ ALA biosynthetic pathway, on the production of 5-aminolevulinic acid (ALA) has been described in cells of Rhodospirillum rubrum N-1. To the Lascelles basal medium the addition of both 30 mM L-glutamicacid and 20 mM levulinic acid (LA) provided to increase the extracellular ALA yield up to 40 fold (76 mg/l). By the addition of both 60 mM glycine and succinic acid, precursorsof $C_4$ ALA biosynthetic pathway, at middle log phase of cell growth ALA yield was increased 27 fold (52 mg/l) although the celt growth was inhibited to a certain extent.
17$\beta$-Estradiol이 수컷 송사리의 성적 행위 및 생식능력에 미치는 영향
류지성,이철우,박응로,남성숙,남규찬,류홍일,전성환,나진균,최덕일 한국독성학회 2001 Toxicological Research Vol.17 No.2
Sexual behavior and reproductivity of male fIsh were studied as an in vivo screening method of endocrine disruptors. Male medaka (Oryzias latipes) were exposed to 17$\beta$-estradiol at nominal concentrations of 2 and 20 $\mu\textrm{g}$/l for 14 days. After exposure of the chemical, sexual behavior between male medaka and normal female which were injected with prostaglandin $F_{2\alpha}$ just before the test, was analysed by using video camera for one hour. Normal control male showed courtship dancing such as following, guarding, dancing and crossing while 17$\beta$-estradiol treated male did not show any type oj courtship dancing. Furthermore, fecundity and fertility were significantly decreased in the treated group. It was suggested that analysis of sexual behavior could be a useful endpoint for the screening of the endocrine disruptors.
구자민,류지성,정규혁,이철우,박응로,박광식 환경독성보건학회 2001 환경독성보건학회지 Vol.16 No.4
Endocrine disruption in crucian carp (Carassius auratus) living in the branch of Han River were examined. Vitellogenin level in plasma was measured using ELISA system and aromatase mRNA level in brain was observed using RT-PCR technique. In all female fish, vitellogenin levels were in the range of 20~40 μg/ml and aromatase mRNA expression could be detected on the agarose gel after RT-PCR. However, in case of males, vitellogenin level was elevated in only one fish, while vitellogenin was hardly detected in others. Aromatase was expressed in all males although the levels were relatively lower than the level in female fish. Testis-ova and any other histological changes of reproductive organ were not shown in both sexes.
남성숙,이철우,류지성,박응로,남규찬,류홍일,전성환,나진균,최덕일,박광식 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.2
To evaluate the arsenic effect of fish endocrine disruption, crucian carp (Carassius auratus) were treated with 0.5 mg/L and 3.0mg/L of As₂O₃ for 14 days and gonadal development was examined by histological analysis. In ovaries from female crucian carps exposed to 3.0mg/L, immature follicles and atretic follicles were appeared while various normal developmental stages of oocytes were shown in control group. Body weight and Gonadosomatic Index (GSI) of treated carp were also decreased compared to control group. However, no other significant histological changes in liver or kidney were shown in this exposure scheme, This means that reproductive organs are more sensitive than other organs and arsenic may exert endocrine disrupting effect through inhibiting the development of reproductive organ in fish.