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랫드의 출생전·후의 위조직 발달에 관한 형태학적 연구 1. 광학현미경적 및 주사전자현미경적 관찰
정헌식,김종섭,이종환,Chung, Heon-sik,Kim, Chong-sup,Lee, Joung-hwan 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.4
The present study was designed to investigate the morphological developments of the stomach in the prenatal and postnatal rats. The gastric fundus of 16- to 22-day-old fetuses, neonates, 7-day-old, 14-day-old, 21-day-old, and adult rats were observed by light and scanning electron microscopy. Light microscopic studies. 1. In the 16-day-old fetuses, the walls of the gastric fundus were differentiated into epithelium, lamina propria, muscle layer, and serosa. The lamina propria was composed of mesenchymal connective tissue cells with sparse nucleus and their fibers. 2. In the 17-day-old fetuses, the muscular layer were differentiated into the circular and longitudinal muscle layers. 3. The epithelium on the fundus of stomach was stratified columnar cells at 16-, 17- and 18-day-old fetuses, but partly converted into simple columnar epithelium at 19-day-old fetuses. 4. The mucous cells were positive by PAS reaction at the 19-day-old fetuses and then these developed rapidly within 1 or 2 days before birth. 5. In the 20-day -old fetuses, the parietal cells were distinguished from other type cells and these cells were chiefly crowded in the middle parts of the gastric glands after 7-day-old. Scanning electron microscopic studies. 6. The surface of gastric mucosa was covered with forms of the various protrusions by forming villi at the last few days before birth and these protrusion forms were fused and folded each other. 7. The mucosal surface was closely packed by polygonal mucous cells. The openings of gastric glands were seen as slit-like round invagination and were more numerous and increased gradually in depth after the postnatal life. The above findings indicate that prominent changes occurred in the pattern of cellular proliferation in the stomach fundus at the end of gestation. The gastric epithelium had well-defined glands composed of parietal, chief and mucous cells just before the birth. The stomach fundus, therefore, were developed morphologically as those of normal adult at fourteen days after birth.
정헌식,김도희,김한수,이영근,성종환,윤광섭,문광덕 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.2
The effects of different deastringency treatments (untreated, carbon dioxide, warm water, or ethanol), before drying on the quality characteristics of dried fruit slices prepared from whole “Cheongdobansi” persimmons were evaluated. L* (lightness) and a* (redness) values of dried slices from warm water- and ethanol-treated groups were higher, respectively, compared to that of dried slices from other groups. Hardness was lower in dried slices from ethanol-treated fruits. Moisture, water activity, soluble solids, titratable acidity, and sensory properties (color, aroma, texture, sweetness, and overall acceptability) of the dried slices from astringency-removed fruits were higher when compared to those of the dried slices from non-treated persimmons. In particular, the dried slices from ethanol-treated fruits showed the highest values for these parameters. Moreover, soluble tannin and DPPH radical scavenging activity decreased by deastringency treatment. Results suggest that ethanol deastringency treatment before drying could be a useful method to improve most quality characteristics, except antioxidative activity, of dried persimmon slices.
정헌식,윤광섭 한국식품저장유통학회 2014 한국식품저장유통학회지 Vol.21 No.3
볶음온도에 따른 옥수수 분말의 흡습특성을 구명하기 위하여, 옥수수 낟알을 160, 180, 200, 220, 240℃에서 20분간 볶은 후 분쇄하고, 20℃에서 각 분말의 수분활성도(aw) 0.11, 0.33, 0.53, 0.75, 0.90에 따른 평형수분함량(EMC)을 측정하여 등온흡습곡선을 작성하였다. 볶은 옥수수 분말의 등온흡습곡선은 aw 증가에 따른 EMC 증가가 전형적인 sigmoid 형태를 보였으며, aw 0.53 이상의 영역에서는 동일 aw에서 볶음온도가 고온일수록 높은 EMC를 나타내었다. 옥수수 분말의 등온흡습곡선은 볶음온도의 뚜렷한 영향이 없이 Oswin, Caurie, Henderson, Halsey 모델로 설명이 가능하였으며, 가장 적합한 모델은 Oswin인 것으로 나타났다. 단분자층 수분함량은 전범위의 aw영역에서 GAB 방정식으로 예측할 수 있었으며, 볶음온도가 높아짐에 따라 증가하는 경향을 보여서 180℃에서는 0.043 kg H2O/kg solids, 240℃에서는 0.053 kg H2O/kg solids을 각각 나타내었다. 이로써 옥수수의 볶음온도가 분말의 20℃ 흡습특성에 영향을 미치는 것으로 확인되었다.
부추의 증숙처리가 착즙액 분무건조 분말의 품질에 미치는 영향
정헌식,김한수,김동섭,이영근,성종환 한국식품저장유통학회 2015 한국식품저장유통학회지 Vol.22 No.3
This study was conducted to develop a spray-dried garlic chives (Allium tuberosum) powder and to evaluate its quality characteristics depending on the treatment of steam blanching pretreatment (100℃, 3 min) and the addition of forming agents (dextrin (DE=10), β-cyclodextrin) during process. The steam blanching pretreatment showed an increase in L* value while a decrease in -a*, b*, C*, and h° values of the powder. Moisture content and water soluble index were not affected by the treatment of steam blanching and the addition of forming agents, whereas the particle diameter was the smallest in the steam blanching treatment and dextrin addition. Chlorophyll, phenolic compound, and vitamin C content, and DPPH radical scavenging activity of non-pretreated powder were significantly higher than those of the steam blanching treated powders. However, there was no significant difference between the two forming agents. The sensory acceptability (color, smell, and overall acceptability) of powder treated with steam blanching were significantly higher than those of non-pretreated powders. Therefore, the steam blanching pretreatment of fresh garlic chives affected on the better quality characteristics of the spray-dried powders when compared with non-pretreated powder though it adversely affected the natural chemical quality of fresh garlic chives.
랫드의 출생전·후의 위조직 발달에 관한 형태학적 연구 2. 투과전자현미경적 관찰
정헌식,김종섭,곽수동,Chung, Heon-sik,Kim, Chong-sup,Kwak, Soo-dong 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.4
The present study was designed to investigate the morphological developments of the stomach in the prenatal and postnatal rats. The gastric fundus of 16- to 22-day-old fetuses, neonates, 7-day-old, 14-day-old, 21-day-old, and adult rats were observed by transmission electron microscopy. 1. In the 19-day-old fetuses, the gastric pits and the parietal cells were the primitive state, but the parietal cells contained numerous microvilli and mitochondria. 2. In the 20-day-old fetuses, the intracellular canaliculus appeared in the parietal cells, and glycogen granules were localized mainly in the basal part of the epithelial cells and parietal cells. 3. In the 20-day-old fetuses, the mucous granules and zonular occludens appeared in the gastric glandular cells adjacent to the parietal cells. 4. In the 21-day-old fetuses, the chief cells were identified in the lamina propria and these cells contained granular endoplasmic reticulum and zymogen granules. 5. In the 20- to 22-day-old fetuses, the definitive parietal cells were firstly appeared in pairs or groups within the lamina propria. These cells tended to be placed near the basement membrane and blood capillary, and frequently tended to be extruded into lumen of gland. 6. The endocrine cells were appeared at the basal areas of the gastric gland after the 14-day-old.
정헌식,문광덕 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.3
The quality of ginger rhizomes packaged in low density polyethylene bags (modified atmosphere packaging - MAP) without perforations (P-0), with 2perforations (P-2), and 12 perforations (P-12) have been investigated for 5 months at 12±1^oC. Sprouting and rotting rates of ginger in P-2 and P-12 were lower than ginger in P-0. Weight loss was higher in P-12 than P-0 and P-2. Surface L^* (lightness) and a^* (redness) values in P-2 and P-12 were lower and higher, respectively, than those found for P-0. Sensory appearance and overall acceptability were rated significantly better in P-2 and P-12 than in P-0. Internal color, firmness, soluble solids, and pH of ginger in MAP were not affected by presence of perforations. Therefore, MAP with the appropriate number of perforations (in this work, 2 perforations) could be a useful method to control sprouting and maintain quality except for surface color of stored ginger rhizomes.