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Refractory Ceramic Fiber와 Rock Wool로 자극한 폐포대식 세포에서 Reactive Oxygen Species 분비와 관련된 세포내 신호변환계
이권행,임영,양경숙,오현숙,김은경,김경아 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.4
Objectives : It is known that the high fibrogenecity of particles is connected with their cytotoxicity for macrophages. Although the molecular mechanism leading to fiber-induced fiber-induced cytotoxicity is still not clear, several mechanism have been suggested. The release of reactive oxygen species(ROS) from activated alveolar macrophages(AM) by dust have been suggested as a possible mechanism of particle-induced cell damage. But the mechanism which man-made vitreous fiber(MMVF) induces the production of ROS in AM is still not clear. In this study, we evaluated the relationship between ROS production and lactate dehydrogenase(LDH) release from alveolar treated with refractory ceramic fiber(RF2) or rock wool(RWI) and signal transduction path-way of ROS production in RF2 or RW1 exposed AM. Methods : We investigated LDH release from MMVF-stimulated AM for index of cytotoxicity. To determine what kind of signal transduction pathways are involved in MMVF-stimulated ROS generation, we used some drugs which have an effect on the signal transduction pathway. Results : RF2 and RW1 induced increase of LDH release with dose-dependent manner with RF2 having greater effect than RW1. There was a dose-dependent increase in the production of ROS by RF2 or RW1. At all level of concentration, RF2 induced more ROS production than RW1. Inhibitors of PKC(bisindolylmaleimide), PLC(U73122 and neomycine) and PTK(genistein and erbstatin) suppressed RF2 or RW1-induced ROS production. Conclusion : There was significant correlation between LDH release and ROS production from AM treated with RF2 or RW1. RF2 and RW1 induced ROS generation through protein kinase C(PKC), phospholipase C(PLC) and protein tyrosin kinase(PTK) pathways.
Ca Ionophore, A23187에 의한 30A-5세포의 지방세포 분화 억제
이권행,윤경하,한만덕 순천향대학교 1993 논문집 Vol.16 No.1
지방세포 분화 억제 과정에서 ??의 역할을 알아보기 위해, 30A-5 preadipocyte가 지방세포로 분화되는 과정에 ?? ionophore, A23187을 첨가하여, 30A-5세포의 지방세포 분화도와 acetyl-CoA carboxylase 활성을 검토하였다. 30A-5 preadipocyte는 insulin과 dexamethasone의 존재하에서 성숙된 지방세포로 분화되는데, 세포가 분화되는 과정에서 1 μM의 ?? ionophore, A23187을 가하면 30A-5 의 지방세포로의 분화가 거의 완전히 억제된다. 30A-5 세포가 지방세포로 분화되는 과정에서 비례적으로 증가하는 지방산 합성의 조절효소인 acetyl CoA carboxylase의 합성은 ?? ionophore, A23187처리에 의해 억제하는데, 30A-5 세포의 지방세포 분화억제는 acetyl-CoA carboxylase합성 저하에 따른 결과로 추측된다. ?? ionophore, A23187에 의한 acetyl-CoA carboxylase 의 합성저하는 비교적 장기간에 걸쳐 나타나는 효과로, 이 억제효과는 ??의 세포막이동의 경쟁적 저해제인 ??에 의해 부분적으로 극복된다. 따라서 A23187에 의한 acetyl-CoA carboxylase의 합성저하와 이에 따른 지방세포로의 분화를 억제하는 과정의 signal tranduction 과정에는 ??의 이동이 중요한 역할을 할 것으로 추정된다. To examine inhibitory effect of ?? ionophore, A23187 on adipocyte conversion of the 30A-5 preadipocytes, we checked the degree of differentiation and acetyl-CoA carboxylase activity of 30A-5 cells in the presence and absence of ?? ionophore, A23187. Inhibition of differentiation of 30A-5 preadipocytes into matured adipocytes was almost completely inhibited by ?? ionophore, A23187. Inhibition of differentiation of 30A-5 cells be resulting from decreased synthesis of acetyl-CoA carboxylase, which is regulatory enzyme in de novo fatty acid synthesis, by ?? ionophore, A23187. Decreased synthesis of acetyl-CoA carboxylase by ?? ionophore, A23187 might be one of the long term effect, and this inhibitory effect was partially recovered by ?? which is competitive inhibitor in membrane transport of ??. Therefore we assumed that ?? mobilization may play a important role on signal tranduction process in inhibitory action of 30A-5 preadipocytes into adipocytes.
급성기 주요우울장애 환자에서 Natural Killer T 세포
박이진,이제훈,이권행,한상익,전양환 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.3
Objectives : To evaluate an association between depression and altered immunity, we examined peripheral T lymphocyte or natural killer (NK) cell measures plasma ACTH and cortisol using the flow cytometry in acute and unmedicated patients with major depressive disorder (MDD). Methods : Forty-two patients with MDD from the outpatient clinic and forty normal controls from the hospital staff were recruited. We applied Hamilton Rating Scale for Depression (HAM-D) and Hamilton Rating Scale for Anxiety (HAM-A) for depressed subjects. Peripheral T lymphocyte or NK cell measures (CD3, CD4, CD8, or CD56) and plasma hormones (ACTH and cortisol) were obtained from all subjects. Results : There were no statistical differences in CD3, CD4, CD8, or CD56 between the two subjects. The number of CD56 cells negatively correlated with HAM-D scores (r= -0.42, P<0.01), but did not correlate with HAM-A scores in patients with MDD. The number of CD56 cells showed strong negative correlation with CD4/CD8 (r= -0.47, P<0.01) in the control group, but not in the depressed group. Patients with MDD had higher cortisol level than controls within the normal range. Conclusion : The trait of immunological imbalance and HPA axis abnormality were shown in patients with MDD. Especially, the severity of depression, but not the anxiety, could be reflected as decreased number of CD56 (NK T) cells in acute and Unmedicated state.
달맞이꽃(Oenothera odorata Jacquin) 뿌리에서 추출한 항균성분의 특성
신성진,권순경,이권행,성낙도,최우영 충남대학교 농업과학연구소 1994 농업과학연구 Vol.21 No.1
An antibacterial component was isolated from the roots of evening primrose (Oenothera odorata Jacquin). The component was purified by repeated preparative TLC of the chloroform-extract with solvent system of toluene(7): ethyl acetate(4) : formic acid(O.8). NMR spectrum indicated that it is a steroid compound containing radical(-C=C-C=0) and aromatic structures. Its antibacterial activity against strains of the genus Streptococcus was relatively high, the MIC value was 50 according to the Hoechst Manual-345, as compared with the other genera such as Staphylococcus and Escherichia.