Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Reliability Considerations of Hybrid Power Systems for Critical Facilities

Choi, Se Jin · Ham, Young Hwan · Kim, Myung Chin 충북대학교 컴퓨터정보통신연구소 2008 컴퓨터정보통신연구 Vol.27 No.2

본문참고

비복신경이식을 이용한 긴 거리의 말초신경 결손부 수복

이종호,이세영,김명진,이은진,안강민,김성민,최원재,명훈,황순정,서병무,최진영,정필훈 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2

The surgery of oral and maxillofacial area poses the risk of cranial nerve damage such as trigeminal nerve or facial nerve. Inferior alveolar nerve is prone to damage in the third molar extraction, implant installation, orthognathic surgery, open reduction and rigid fixation, and tumor ablation surgery. On the other hands,facial nerve is likely to be damaged or sacrificed with trauma or parotidectomy. In case of inferior alveolar nerve injury, the incidence is reported to be about 1.3%. The nerve function will almost recover in minimal damage, but it won't recover at last in total damage of a part of nerve unit. In latter cases, nerve regeneration in intended by allograft as nerve substitute or various route of merve condit. But the recovery with autograft is believed to be most relialbe mrthod in the rapair of long-span(longer than 15㎜)nerve defect. We have performed autologous sural nerve graft in the repair of nerve defect, which is caused by resection of benign or malignant tumor. Hereby we report the method of nerve harvesting, recovery of defected peripheral nerve and the complications of donor site with the discussion of sural nerve anatomy.

工兵 學校 環境敎育 時間에 대한 實態 分析

최명규,송창수,양회진,김창환 호남대학교 산업기술연구소 1999 산업기술연구논문집 Vol.7 No.-

본 연구는 군 환경 교육의 근간이 되고 있는 육군공병학교를 대상으로 군 환경교육의 실태를 조사, 분석하여 문제점을 도출하고 그 개선방안을 제시함으로써 효율적이고 실질적인 환경교육이 이루어질 수 있도록 하고, 나아가 군의 환경관리 능력을 제고시키는데 연구의 목적을 두었으며, 공병학교에서 교육중인 군인들을 조사대상으로 삼아 설문조사를 실시하여 분석해 본 결과는 다음과 같다. 1. 군 환경 교육의 필요성에 대해서는 95%의 응답자가 필요하다고 응답하여 군 환경 교육의 필요성에 대하여 공감하고 있는 것으로 나타났으며, 군에서의 환경교육은 사회와는 다른 교육이 되어야 한다고 느끼고 있다. 2. 군에서 실시되고 있는 환경교육에 대해서는 불만족도가 높은 것으로 나타나 군의 환경교육 방법과 방향에 있어서는 많은 개선이 필요한 것으로 나타났다. 3. 응답자의 절반 이상이 군 생활 중 4시간 이상 환경교육을 받는 것으로 나타난 반면 그 내용과 질에 있어서는 사회의 환경교육과 비교하여 불만족하고 있는 것으로 나타나 보다 폭 넓은 분야에 대한 환경교육이 이루어져야 할 것으로 판단되었다. 군 환경교육의 최적시간에 대한 설문에서는 장교 과정은 고군반은 현재의 8시간이, 초군반은 현재의 12시간보다는 4일 이상이, 그리고 특기병 과정은 2주일 이상이 적당할 것으로 나타났다. The purpose of this study was to research on the actual condition of the environmental education in Army Engineer's School. The major findings of this study were as follows; 1. 95% of soldiers answered "positive", that the environmental education is needed in the Army. And they also wanted there must be some differences between Army and Civilian in the environmental education. 2. Soldiers who are highly educated officers were more "dissatisfacted" with the environmental education in the Army than private. So improvement must be made in the Army environment education both the way direction and education. 3. The soldiers have not learned environmental education of half of responsers, most of them are dissatifacted with both contents and quality of the environmental education.

의류 중의 미생물에 대한 소비자의 지식과 세탁습관 실태조사 분석

최해운,정찬진,박명자 服飾文化學會 2002 服飾文化硏究 Vol.10 No.6

Microorganisms living in clothing cause damage to fabric as well as unhygienic conditions with unpleasant odor for wearers. Removal or growth of microorganisms are affected by the conditions during washing and storage. The purpose of this research was to study the consumer's knowledge and habits in laundering with respect to microorganisms in clothing. For survey method, questionnaires were administered to 580 housewives, age of 20~60s living in Seoul. Employing 479 respondents, the data were analyzed by using deseriptive statistics. The results are as follows; The level of knowledge about microorganisms of clothing was high in general, but wasn't expert level. Many people had experienced damages of textiles, clothing and unpleasant odor due to microorganisms. Fabric softeners and bleaches were rarely used for disinfection but usually used for antistatic. whitening or removal of stains. There was no relationship between laundering habits the knowledge of microorganism, and experience of clothing damage by microorganism

석탄회 펠렛의 미연탄소 연소 현상 분석

崔尙丁,李孝眞,吳明淑 弘益大學校 科學技術硏究所 2001 科學技術硏究論文集 Vol.12 No.-

As the number of coal fired power plants in the domestic power industry continues to increases, the amount of fly ash produced is also increasing, making the reutilization of fly ash a vital issue. Various methods to reutilize the fly ash from coal power plants have been investigated, and one of such areas with the potential to grow is the usage as light weight sintered construction materials. Sintered fly ash aggregates are produced in two steps; the first is the pellet formation step, during which the fly ash particles containing unburnt carbons were made into a pellet using caking components, and the second the pellet sintering by the combustion heat of the carbons. During the sintering process, the combustion rate of the carbon affects the characteristics of sintered pellets. The combustion of carbon occurs via adsorption of O₂ on the active sites, and desorption of CO/CO₂. The parameters which affect the rate of adsorption, desorption, and transfer of O₂ and CO/CO₂ include the O₂ partial pressure and flow rate, the pellet size, the porosity, the pore tortuosity, the pore size distribution, the carbon content and distribution. This study investigated the combustion of carbon during the production of sintered fly ash through modeling. A simple combustion model using Langmuir isotherm is used to calculate the pellet temperature. The oxygen diffusion through the pores is ignored. At the temperatures of sintering, the desoption process is a rate limiting process, making the overall rate sensitive to the desoption kinetic parameters. In addition, it was found that the carbon content and the heat capacity of the pellet affected both the rate of the reaction and the final pellet temperature, while the area of active sites only affect the reaction rate. The future work will include the combustion modeling with external and internal diffusion of O₂, and the model confirmation through experimentation.

유속과 곡관부의 형상 변화를 고려한 파이프 계의 자유진동 해석

최명진,임경식,이동명 경희대학교 산학협력기술연구원 2000 산학협력기술연구논문집 Vol.6 No.-

A simulation is performed to study the change of the natural frequencies of curved pipe systems containing fluid flow, for different angles and geometry of the pipe systems. Based upon the Hamilton's principle, the equations of motions are derived. This paper consider an initially deformed state caused by the fluid flowing through the pipe at a constant velocity. This deformed state gives rise to initially tension force in the pipe. When the initial force is neglected in cured pipes, the natural frequencies are reduced as flow velocity increases. However, when the initial force is considered, the natural frequencies changed a little with the change of the flow velocity for changing shape(curvature, angle) of curved pipes. In free vibrational simulation of pipe systems, it is necessary to consider the initial force due to the flow velocity and the geometric shape of pipes. Like this, the initial force should be included in the equation of motion of the systems to get more accurate natural frequencies and the change of shape of curved pipes should be considered.

復合材料 連結 部位를 갖는 엔진 마운트 設計에 關한 硏究

최원종,김권진,오인택,임동준,홍영길,황명신 한국항공대학교 1993 論文集 Vol.31 No.-

본 논문에서는 초경량 항공기의 엔진 마운트 구조물에 대한 유한요소 해석을 수행하였으며, 구조의 안전성 확보에 중요한 영향을 주는 부분인 복합재료 동체와 엔진 마운트 간의 연결 부위에 대한 해석을 위해 유한요소 프로그램을 작성하였다. 본 연구에 의해 작성된 유한요소 프로그램의 신뢰성을 확인하기 위해 실제로 시편 제작 실험을 실시하여 실험치와 해석치를 비교 검토하였다. The finite element analysis was performed on the ULM's engine mount to verify the structural safety Result showed that proposed engine mount structure has a large margine of safety. Strains and stresses in the vicinity of the joint between a composite fuselage and a metallic engine mount were calculated by the finite element analysis program. Numerical results from the model were compared with the test results. Good agreement was found between experiment and model results.

Ba_2Ti_9O_20 유전체 재료의 저온소결 첨가제에 의한 소결 거동 및 고주파 특성

최명호,이주희,박명진,고영철,김남철 국립7개대학공동논문집간행위원회 2001 공업기술연구 Vol.1 No.-

Sintering characteristics and microwave dielectric properties of the Ba_2Ti_9O_20 ceramics added with lithium-boro-silicate glass frit were investigated. Sintering experiments showed that the densification occurred at lower firing temperatures down to 900℃ as the addition of glass frit was increased up to 10wt%. The bulk density and shrinkage of ceramics were strongly dependent on the amount of the glass frit. The microstructures of the ceramics were found to become skeletal-like structures as increase of glass frit. The monoclinic crystal structure of the Ba_2Ti_9O_20, however, was found not to be affected by the addition of glass frit up to 10wt%. Microwave dielectric constants of the glass frit added ceramics were relatively consistent of 35 with over 15% shrunk and densified ceramics. However, the quality factor (Qf) and the temperature constant (TCP) was found to be significantly affected by the additions of glass frit.

코로나 플라즈마 처리에 의한 HDPE 표면의 개질에 관한 연구

최진숙,전종탁,정봉규,이근대,문명준,이종무 釜慶大學校 2001 釜慶大學校 論文集 Vol.6 No.-

We are able to generate the stable plasma in open air and nitrogen using a corona plasma treatment system(CPTS) with a high voltage. This was applied to treat the surface of rather non-polar HDPE(High Density Polyethylene) intended for the improvement of adhesion of over-coated layers such as coatings, printing inks. The surface tension of HDPE was significantly improved by the CPTS. The characteristics of surface layer were evaluated by means of contact angle measurement, ATR-FT/IR spectroscopy, and it was found that functional groups were introduced into the surface layer of the HDPE. The use of nitrogen was increased the surface tension of the HDPE from 33.8 mJ/m^2 to 66.56 mJ/m^2, and highly effective method of HDPE surface modification could be realized.