한국(韓國)GSK 상임고문최현식(常任顧問崔賢植)의 기업활동(企業活動)과 경영이념(經營理念)

박광서 ( Kwang Sur Park ),최승현 ( Seung Hyun Choi ) 아시아.유럽미래학회 2006 유라시아연구 Vol.3 No.2

The aims of this paper are to review Mr. Choi Hyun Sik``s business activities and analysis of his management thoughts. Mr. Choi Hyun Sik is now a general advisor of Galxo Smith Cline(GSK) Korea, one of the biggest pharmatical company in the world. And he is one of the toppest chief executive officer in Korea. He was passed as a semi-salesman, one of 3D jobs at that time, by the Choong Wae Pharamatical Corporation in 1966 and he met Mr. Lee Chong Ho who was a section chief of planning and management section anf he is now chairman of the corporation. He worked hard with chairman Lee. At last he promoted as a CEO of Choong Wae Pharmatical Corporation in 1998 and Vice Chairman of the Company in 2004. After 38 years services, he resigned from CW in 2004. At the same time, He was selected as a general advisor of GSK Korea(President Mr. Kim Jin Ho) on April 2004. As a GA of GSK Korea, As a matter of fact, Mr. Choi concertrate into his best efforts as the fellowing points; ● Focus an execution of plan. ● Develop new strategic business opportunities ● Strengthen partnership with external stakeholders. ● Optimize commercial structure and capability ● Improve working capital management & operational excellence ● Build organizational capabilities to support growth. ● Develop people pipeline, and etc. And last, his management thoughts and stratagies are 5S and 5C. That is, 5S means Speed, System, Simplicity, Strategy and Spurt. On the other hand, 5C are Creation, Credit, Competition, Customer and Communication.

Effect of Graphene on Growth of Neuroblastoma Cells

( Park,Hye Bin ),( Hyo Geun Nam ),( Hong Gi Oh ),( Jung Hyun Kim ),( Chang Man Kim ),( Kwang Soup Song ),( Kwang Hwan Jhee ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.2

The unique properties of graphene have earned much interest in the fields of materials science and condensedmatter physics in recent years. However, the biological applications of graphene remain largely unexplored. In this study, we investigated the conditions and viability of a cell culture exposed to graphene onto glass and SiO2/Si, using a human nerve cell line, SH-SY5Y. Cell viability was 84% when cultured on glass and SiO2/Si coated with graphene as compared with culturing on polystyrene surface. Fluorescence data showed that the presence of graphene did not influence cell morphology. These findings suggest that graphene may be used for biological applications.

國內産 茶葉의 脂肪酸 組成에 관한 硏究

朴章鉉(Jang-Hyun Park),金正鳳(Jung-Bong Kim),金廣植(Kwang-Sik Kim) 한국차학회 1996 한국차학회지 Vol.2 No.1

Fatty acid composition was analyzed and compared in tea shoot of korean native tea plant (Camellia sinensis Var. Sinensis) picking from thirteen places and Yabukita(Camellia sinensis Var. Yabukita), an excellent Japanese variety. Results of this investigation are summarized as follow. Palmitic acid, Stearic acid, Oleic acid, Linoleic acid, Linolenic acid were confirmed thirteen places of korean native tea plant. The content of total fatty acid at Shunchang-gun tea leaves usually was the highest as 3555.3mg/100g, and that of Yong Kwang-gun tea leaves was the lowest as 2089.1mg/100g. The content of total fatty acid of Yabukita tea leaves usually was 3084.9mg/100g. The composition of fatty acid had order of C18 : 3 > C18 : 2 > C16 : 0 > C18 : 1 > C18 : 0.

구강 내 미생물에 대한 서양산 고추냉이(Horseradish, Armoracia rusticana) 뿌리 천연추출물과 합성 Allyl isothiocyanate의 항균활성 비교

박광선,박호원,신일식,이주현,서현우 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.2

서양산 고추냉이(Horseradish, Armoracia rusticana)의 항균 성분 중 주 성분인 Allyl isothiocyanate(AIT)는 천연물을 이용한 추출이나 유기합성을 통해서 얻을 수 있으며, 이제까지 사용되고 있는 항균제품 및 식품첨가물들은 주로 유기합성법에 의해서 얻어졌다. 이전 연구에서 서양산 고추냉이 천연추출물의 다양한 구강 내 미생물에 대한 항균활성은 보고된 바 있으나, 유기합성한 AIT용액의 구강 내 미생물에 대한 항균활성은 아직까지 연구된 바가 없다. 이에 본 연구에서는 유기합성을 통해 얻은 AIT 용액과 서양산 고추냉이 뿌리 천연추출물의 구강 내 미생물에 대한 항균활성을 평가하였다. 항균활성을 평가하기 위해 최소억제농도와 최소살균농도를 측정하였으며, 그 결과는 다음과 같다. 1. 본 연구에 사용된 7종의 구강 내 미생물에 대한 서양산 고추냉이 뿌리 천연추출물의 최소억제농도는 약 117~1,750 ppm(0.0117~0.175%)이었으며, 유기합성한 AIT용액의 최소억제농도는 약 344~3,000 ppm(0.0344~0.3%)으로 모든 균주에 대해 항균활성을 나타내었다. 2. 본 연구에 사용된 7종의 구강 내 미생물에 대한 서양산 고추냉이 뿌리 천연추출물의 최소살균농도는 약 625.2~6,000 ppm(0.06252~0.6%)이었으며, 유기합성한 AIT용액의 최소살균농도는 약 1,750~7,000 ppm(0.175~0.7%)이었다. Allyl isothiocyanate(AIT), the principle ingredient of antimicrobial ingredients from horseradish root, can be prepared from extracts of horseradish root or synthetic method. It is reported that the horseradish root extract has the antimicrobial effect against various oral microorganisms, while there is no further study about the antimicrobial effect against the oral microorganisms of synthetic AIT derived from synthetic method. The aim of the study is to compare the difference of the antimicrobial effect between horseradish root extracts and synthetic AIT. To evaluate the antimicrobial effect, we measured the minimum inhibitory concentration(MIC) and minimum bactericidal concentration (MBC), and the results are like following. 1. The MIC of horseradish root extract against 7 kinds of oral pathogenic microorganisms is about 117~1,750 ppm(0.0117~0.175%), and the MIC of the synthetic AIT is about 344~3,000 ppm(0.0344~0.3%), which have the antimicrobial effects against all kinds of microorganisms. 2. The MBC of the horseradish root extracts against the 7 kinds of oral microorganisms is about 625.2~6,000 ppm(0.06252~0.6%), and the MBC of the synthetic AIT is about 1,750~7,000 ppm(0.175~0.7%), which have the antimicrobial effects against all kinds of microorganisms.

보건관리 대행사업 평가도구의 타당성, 신뢰도 및 인과관계 검증에 관한 연구

박정일,조병희,구정완,김광종,김현욱,원종욱,이명숙,정문희 가톨릭대학교 산업의학센터 2001 韓國의 産業醫學 Vol.40 No.1

In order to verify the validity, reliability and relativeness of preliminary evaluation tool for the group occupational health services(GOHS), a questionnaire survey for doctors, nurses and hygienists of 18 GOSH institutions, and employers, health managers and employees of 340 enterprises was conducted. A factor analysis and correlation test for the statistical validity of the variables in the evaluation tool, a Chronbach's Alpha value for the reliability in the evaluation indices and a regression analysis for the relations of the evaluation model were performed. The results were as follows: 1.In factor analysis, thirty-three variables among 43 variables were selected as significant variables, ie 9 out of 11 for input index, 11 out of 14 for throughout index and 13 out of 18 for out put index. 2.Three evaluation(outcome) scales(evaluation by employer, health manager and employee) which composed of 16 identical items were used. 3.The Chronbach's Alpha values of input, throughout, out put and outcome indices were 0.5221, 0.5473, 0.7122 and 0.8546 respectively. 4.Regression equations of independent variables on the evaluation scales of employer, health manager and employee were all statistically significant.

위암세포에 의한 종양침윤 림프구의 면역반응 억제기전에 관한 연구

박정규,송규상,서광선,최정목,배진선,장일성,윤완희,노승무,조은경,백태현 大韓免疫學會 1995 大韓免疫學會誌 Vol.17 No.3

Tumor-infiltrating lymphocytes ('1°ILs) interact most closely with tumor cells and thus are more likely to reflect tumor host interactions accurately. But it is unknown whether such T cells are nonspecific inflammatory cells or a subset of specific host immune responses. In this study, there was no clear correlation between the infiltration of T lymphocytes in stomach cancer and the overexpression of c-ErbB-2 or increasing class I MHC expression on tumor cells. A positive correlation was seen between the presence of TILs in the tumor and tumors with diploidy by flow cytometric DNA analysis. The proliferative responses of Ills stimulated with IL-2, anti-CD3 mAb, or both were examined. When compared to normal mucosal-associated lymphoid tissue lymphocytes, the proliferative response of TILs to high dose IL-2 was minimal. A similarly poor response to anti-CD3 mAb plus IL-2 was also observed. The freshly isolated TILs exhibit reduced ability to proliferate in response to IL-2, anti-CD3 mAb or both. The microenvironment of the tumor suppresses the proliferative capacity of the TILs. The mechanism of this suppression remains unknown. It could be mediated by suppressor cells, by soluble substances within the tumor, or both. To examine this question, supernatants of stomach cancer cells (SNSNU-1) were tested for the presence of immunosuppressive factors. Human peripheral blood T-cells and tumor-draining lymph node lymphocytes (TDLNL) were incubated for 3 days with SNSNU-1 and then assessed for proliferative responses to PMA, anti-CD28 mAb, or both and for the inducibility to express IFN- r or IL-4 mRNA to PMA. Peripheral blood T-cells pretreated with SNSNU-1 were unable to proliferate in response to PMA, anti-CD28 mAb or both. SNSNU-1 also produces inhibitory activities of TDLNL proliferative response to PMA or anti-CD28 mAb and PMA (49%, 52%, respectively). In contrast, culture supernatants obtained from HEp-2, K562 or Daudi showed normal proliferative responsiveness of peripheral blood T-cells and TDLNL by PMA, anti-CD28 mAb or both.

결핵균 30 kDa 항원과 Triton X-100 Solubilized Protein 항원에 의한 대장암 주변 림프절 단핵구의 활성화

박정규,김광호,조은경,임재현,민들레,송영자,김화중,백태현 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Tumor-draining lymph node mononuclear (TDLMN) cells are specifically sensitized to the growing tumor but such cells are deficient for mediating an antitumor response. In this study, we examined the feasibility of using mycobacterial 30 kDa or Triton X-100 solubilized protein (TSP) antigen to stimulate mononuclear cells of colon cancer-draining lymph node for the generation of cell mediated immune effector cells. The proliferative response of TDLMN cells stimulated with mycobacterial 30 kDa or TSP antigen was determined by ^(3)H-thymidine incorporation assay. The proliferation of TDLMN cells to mycobacterial 30 kDa or TSP antigen was significantly increased in PPD (+) patients, but a poor response to the 30 kDa or TSP antigen was observed in PPD (-). The expression on γδ T cells to mycobacterial 30 kDa or TSP antigen was assessed by flow cytometry. The γδ T cells from PPD ( + ) patient responded only to 30 kDa antigen but to TSP antigen. An investigation of cytokine mRNA expression was undertaken using reverse transcription-polymerase chain reaction (RT-PCR) to follow TDLMN cells stimulated with the 30 kDa or TSP antigens for 5 days. The IFN-γ and TNF-α mRNA expression was only induced in TDLMN cells of PPD ( + ) patient in response to the 30 kDa or TSP antigen. The IL-2 mRNA expression was induced in both PPD (+) and PPD (-) in response to the 30 kDa or TSP antigen. But the IL-4 mRNA expression was not induced in response to the 30 kDa or TSP antigen. These results suggest that the 30 kDa and TSP antigens may serve as biologic response modifier for the generation of cell mediated immune effector cells.

PelB Signal Sequence로 유도된 제조합 인간 상피세포 증식인자 분비 발현 벡터의 제조

박세철,남정현,김정근,권태종,고인영,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

전사개시 단계중에 삽입되는 N말단의 methionine이 제거되고 pelB signal sequence를 삽입하여 인체 epidermal growth factor (hEGF)가 직접 분비발현 되도록 고안된 T7과 tac promoter에 의하여 각각 발현되는 pYHB101과 pYHB2를 제조하였다. 또한 T7 promoter에 의하여 발현되지만 signal sequence를 삽입하지 않은 pYHB1도 제조하여 각각 제조된 재조합 plasmid들의 발현 및 분비효율을 비교하였다. pelB signal sequence hEGF 유전자를 갖도록 제조된 pYHB101을 포함하고 있는 균주가 pYHB1보다 균체 성장속도는 낮았지만 pYHB101을 1 mM IPTG로 5시간 induction 하였을 때 13 mg/l의 rhEGF가 분비발현되어 T7 promoter의 발현 체계를 갖는 경우가 tac promoter보다 높은 발현 효율을 보였다. 이때 pYHB101에서 발현되는 rhEGF의 발현량은 준최적 조건에서 14 mg/l이었다. We have designed nucleotide sequences of hEGF structural gene to eliminate the N-terminal methionine residue incorporated during the translation initiation step, and constructed recombinant human epidermal growth factor (rhEGF) secretion plasmids pYHB101, and pYHB2 in which pelB signal sequence-hEGF gene was expressed under the control of the T7, and tac promoter, respectively. We also constructed pYHB1 vector which contains rhEGF gene controlled by T7 promoter. The transformant with pYHB101 showed relatively slow growth pattern compared to the transformant with pYHB1. However, we observed that the transformant with pYHB101 secreted rhEGF of 13 mg/l significantly after 5 hr induction with 1 mM IPTG and that the T7 promoter was more effective than tac promoter when connected to pelB signal sequence. The amount of rhEGF was 14 mg/l under the sub-optimized condition.

서울대학교 치과병원 장애인진료실의 외래환자마취 실태 분석

박창주,정준민,김현정,장기택,이상훈,염광원 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.1

치과적 장애인이란 치과진료 시 치과의사에게 자발적인 협조가 힘든 사람을 의미한다. 본 연구는 서울대학 치과병원 장애인진료실에서 치료받은 치과적 장애인 환자들의 임상진료 실태를 마취과적으로 분석하고 향후 장애인 치과치료에 대한지침 을 마련하고자 하였다. 1999년 1월부터 2002년 10월까지 서울대학교 치과병원 장애인진료실에 내원한 장애인 환자들을 대상으로 조사한 결과, 총89명의 환자들에게 93건의 치과진료가 시행되었다. 환자의 대다수는 정신지체였고 전신마취 하 보존치료를 받았다. 주로 마취유도제는 thiopental, 근이완제는 vecuronium을 이용하였으며 산소, 아산화질소, enflurane을 함께 흡입시켜 마취를 유지하였다. 총 마취지속시간과 회복실 체류시간은 각각 164.4±57.2 분과 106.2±50.5 분이었고 회복 과정에서 심각한 합병증은 관찰되지 않았다. 본 연구는 외래환자마취에 기반한 서울대학교 치과병원 장애인진료실의 치료방침에 따라 특별한 문제없이 성공적인 마취가 가능하였음을 보여 주었다. 또한 증가하는 장애인 치과치료에 대한 수요를 충족시키기 위해서 적절한 시설과 인력을 갖춘 보다 많은 외래환자마취 센터의 필요성도 제시한다고 할 수 있다. Dentally disabled person means one who have difficulty in cooperating voluntarily with a dentist for routine dental care. Seoul National University Dental Hospital (SNUDH) Dental Clinic for Disabled has worked for them under outpatient anesthesia concept. The aim of this study was to determine the anesthetic characteristics of the patients attending for dental treatment at SNUDH Dental Clinic for Disabled in order to establish better future treatment plans. The data were drawn from the patients who visited SNUDH Dental Clinic for Disabled from January 1999 to October 2002. Total 93 dental treatments for 83 patients were enrolled in this study. Most patients had mental retardation and the conservative treatments were carried out under general anesthesia. Thiopental and vecuronium were mostly used for induction agent and neuromuscular blocker, respectively. Enflurane, with oxygen and nitrous oxide, was mostly used to maintain the anesthesia. Mean total anesthetic time was 164.4±57.2 minutes and mean duration at the post-anesthetic care unit was 106.2±50.5 min. Serious postoperative complications were not observed. These results showed our successful anesthetic outcomes without any severe side effect or complication and the needs of more outpatient centers for dental care for disabled.

SR 버퍼할당 메카니즘에 관한 연구

박성헌,김훈,박세환,최현호,박광채 조선대학교 에너지.자원신기술연구소 1997 에너지·자원신기술연구소 논문지 Vol.19 No.2

Since services in ATM networks are very various and the characteristic of traffic from services is very different from each one, shared buffer mechanism, that shares only one buffer, cannot satisfy QoS, requirement for traffics. Furthermore, it has more difficulty in controlling composite cells in buffer when large ATM switch is implemented. In this paper, we propose cell service scheduling of separated routing allocation mechanism that allocates respective, independent buffer to cells with same traffic characteristic according to priority. We analyzed its performance using computer simulation. Then, we obtained cell loss probability changing buffer size and weighted value, when cell service scheduling is done by unsymmetrical round-robin depending on weighted value that is allocated to respective buffer.