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권태종,채호철,한창수,정진태,안강호 한국생산제조학회 2000 한국공작기계학회지 Vol.9 No.6
Spinning mechanism is generally used in coasting process on grass plates. Rebounding PR(Photo Resist) which leads to occur inferiority of coating process is caused by vibrational energy of whole coating system. In this study, the sensitivity analysis is performed to analyze and reduce vibrational terms in the spin coating system. The sensitivity analysis is bared on the numerical expression of this system. By the bond graph method. power flow of each system is represented by some basic bond graph elements. Any energy domain system is modeled using the unified elements. The modelled spin coater system is verified with power spectrum data measured by FFT analyzer. As the results of verifying model parameters and sensitivity analysis, principal factors causing vibration phenomenon are mentioned. A study on vibration method in the spin coating system is discussed.
권태종,이주경,이상훈 한국미생물 · 생명공학회 1990 한국미생물·생명공학회지 Vol.18 No.5
Hydantoinase(EC3.5.2.2) 생산을 위해 토양에서 분리한 균주 Y -183을 배양시킨 결과 Glycerol이 탄소원으로 양호했으며, 질소원으로는 혼합된 형태의 질소원이 요구되었다. 특히 Yeast ext.와 Beef ext.가 효소생성에 크게 영향을 미쳤고, Hydantoin 유도체 및 Pyrimidine 유도체의 영향을 조사한 결과 Uracil이 가장 양호한 결과를 나타냈다. 배양시간은 방선균의 특성상 72시간의 배양이 필요했으며 효소의 생성은 24시간 이후에 왕성하였다. 생성된 Hydantoinase는 균체내에 효소를 축적했으며 이들은 추출하여 기질로서 PL-5-phenylhydantion 등과 반응시킨 결과 N-carbamyl-D-amino acids가 생산되는 것이 분석결과 확인되었다. In order to investigate hydantoinase-producing strain of the genus Streptomyces, 523 strains of Streptomycee sp. isolated from soils were cultivated in various media and conversion activity of the enzyme was measured to DL-5-phenylhydantoin. A number of strains producing hydantoinase were detected and among them, the strain of Streptomyces sp. Y-183 was selected as a most powerful strain to producing the enzyme. The optimal culture conditions for the production of hydantoinase of the strain were studied, and it was found that almost all hydantoinase activity was produced in the cell fraction. The maximum activity of the enzyme, 17.8 unitstg of dried cells weight, was obtained when the strain was cultured at $30^{\circ}C$ for 72 hr in a medium containing 1.0% of glycerol. 0.5% of yeast extract. 0.5% of soytone, 0.5% of beef extract, 0.6% of KCI, 0.002% of $K_2HP0_4, 0.25% \;of \;CaC0_3, \;0.0002% \; of \; ZnSO_4, \; 0.0002%\; of\; FeS0_4$, and 0.4% of uracil as an inducer, and the pH of culture broth was adjusted ranging from 7.0 to 7.5.
權泰種 건국대학교 1980 學術誌 Vol.24 No.2
A strain of Aspergillus species (C-58), isolated from 52 kinds of soil sample, was found to produce a strong inulase in the culture filtrate. Selected strain produced extracellular inulase when cultured in a medium containing not only glucose but also pentose, hexose and inulin, respectively. And obtained results were as in the following. 1.Selected strain was identified to Aspergillus species. 2.Culture filtrate is responsible for the hydrolysis not only of the simple β-fructoside such as sucrose but also of the polysaccharide, inulin. 3.The most effective starting concentration of glucose was 1.5% and reasonable period of shaking culture was 8 days in the preculturing. 4.The enzyme production was found to increase significantly with the addition of corn steep liquor (1.0%), NH4H2PO4 and (NH4)2PHO4 for the nitrogen sources to the medium. 5.The enzyme production was increased with the addition of NaCl, KCI and MgSO4 7H2O for the salts. 6.Optimum initial pH for the production of inulase by shaking culture was 5.0. 7.The addition concentration of CaCO3 was 1.0% after 24 hrs cultivation in the cultures.
방선균(Streptomyces sp. No-78)이 생산하는 α-Amylase에 관한 연구
권태종 建國大學校 附設 産業技術硏究所 1988 논문집 Vol.13 No.-
방선균으로부터 전분분해효소 생산균을 선별하기 위하여 57점의 토양시료로부터 217균주를 분리하여 α-amylase 생산력이 우수한 균주를 선정하여 형태학적 특성 및 효소생산에 미치는 배양조건을 검토하였다. 1. 공시균은 형태학적 특성을 조사하여 본 결과 Streptomyces속의 균이었다. 2. 효소생산에 영향을 미치는 조건으로는 탄소원으로 2% maltose를 사용하였을 때가 좋았고, 유기질소원으로는 casein을 0.6% 첨가 때가 높았으며 amino산으로는 L-methionine과 L-tryptophan의 경우가 높았다. 그리고 금속이온으로는 2가 금속인 Mg, Zn 이온에 의하여 약 20% 증가되었다. 효소생산은 33℃ 부근 및 pH7∼8의 경우가 가장 높았으며 5일간 배양에 의하여 최고로 많은 양의 효소가 생산되었다. A strain of Actinomycetes capable of producing α-amylase was isolated from soil, and the morphological characteristics and the conditions for the enzyme production were investigated. The microorganism was identified as Streptomyces sp. (No-78) on the morphological characteristics. For the enzyme production, 2% maltose and 0.6% casein as carbon and nitrogen source, respectively. The enzyme production was increased by the addition of L-methionine, L-tryptophan, magnesium and zinc ions. Optimal initial pH and temperature for the enzyme prouction was pH 7∼8 and around 33℃, respectively. And the production of the enzyme reached at maximum after 5 days cultivation.
權泰種 건국대학교 부설 산업기술연구소 1984 논문집 Vol.9 No.-
土壤에서 分離, 選別한 streptomyces屬의 1菌株(streptomyces sp. no.-82A)가 生産하는 β-lactamase의 最適生産造件을 검토한 결과는 다음과 같았다. 효소생산에 있어서 탄소원으로는 1% dextrin, 유기질소원으로는 0.2% asparagine이 가장 우수하였으나 무기질소원은 효소생산을 증가시키지 않았다. amino acid로는 L-histidine 0.1%첨가에 의하여 150%증가 되었으며 항생물질로는 cephalexin 및 phenoxymethyl penicillin 첨가에 의하여 각각 28% 및 14%증가 되었다. 금속염으로는 CaCl₂, CoCl₂및 MgSO₄에 의하여 약 40%증가하였다. 최적 배양온도는 32℃였으며 정치 배양량보다 진탕배양에 의하여 약 40%효소생산이 증가되었으며 배양일수도 단축되었다 Optimal condition for the production of β-lactamase obtained from a strain of Streptomyces sp. no.-82A isolated from soil was tested. For the production of the enzyme, 1% dextrin as carbon source and 0.2% L-asparagine as nitrogen source were most favorable, but other inorganic nitrogen sources were not suitable. The enzyme production was increased by the addition of 0.1% L-histidine, cephalexin and phenoxymethyl penicillin to 150,28, and 14%, respectively. And metal salts such as CaCl₂, CoCl₂and MgSO₄were also increased enzyme production to about 40%. Optimal temperature for the production of the enzyme was 32℃. And by shaking culture, enzyme yield was increased to about 40% in comparison to stationary culture, and cultivation period was also shortened.
권태종,채호철,한창수,정진태,안강호 韓國工作機械學會 2000 한국생산제조학회지 Vol.9 No.6
Spinning mechanism is generally used in coating process on grass plates. Rebounding PR(Photo Resist) which leads to occur inferiority of coating process is caused by vibrational energy of whole coating system. In this study, the sensitivity analysis is performed to analyze and reduce vibrational terns in the spin coating system. The sensitivity analysis is bared on the numerical expression of this system. By the bond graph method, power flow of each system is represented by some basic bond graph elements. Any energy domain system is modeled using the unified elements. The modelled spin coater system is verified with power spectrum data measured by FFT analyzer. As the results of verifying model parameters and sensitivity analysis, principal factors causing vibration phenomenon are mentioned. A study on vibration reduction method in the spin coating system is discussed.