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성인 지역사회 폐렴의 원인 미생물에 대한 전향적 다기관 연구 : Legionella, Leptospira, Hantaan virus and Orientia tsutsugamushi
김민자,정희진,손장욱,심희선,박대원,박승철,우준희,강재명,김유겸,신완식,김양리,이환종,김지희 대한감염학회 2001 감염 Vol.33 No.1
Background : Despite rigorous investigations, the etiology of community-acquired pneumonia remains unknown in about 50% of hospitalized patients. The diagnosis of the etiological agent is becoming more challenging and more critical as number of newer pathogens have been recognized in recent years. In the 3-year period prospective study we investigated adult patients with community-acquired pneumonia for Legionella, Leptospira, Hantaan virus and Orientia tsutsugamushi as potential etiologic agents. Methods : A prospective multicenter study was performed from May 1997 to April 2000. A total of 431 patients with community-acquired pneumonia under the inclusion criteria were examined for specific microbial diagnosis; sputum culture and PCR, and serologic teats including indirect immunofluorescence antibody (IFA) test for Legionella, and hemagglutination tests for Leptosoira, Hantaan virus and O. tsutsugamushi. Etiologic diagnosis was determined on the basis of the review of case record forms and specific laboratory diagnostic criteria. Results : During the study period a total of 385 sputum and 283 serum samples were examined. Legionella pneumonia was diagnosed in 2.3% (10/431) of the cases examined : 1.4% cases with PCR-positive (5/367) and 2.1% with positive IFA test (6/283). Leptospirosis and scrub typhus were diagnosed in 0.4% (1/252) and 2.0% (5/252), respectively. All 5 cases with scrub typhus occurred in late fall, and rash or eschar was not found. None of cases was Hantaan virus infection. Conclusion : The results suggest that Legionella, Leptospira, and O. tsutsugamushi should be considered in the etiologic diagnosis and empirical antibiotic therapy of community-acquired pneumonia. (Korean J Infect Dis 32:24∼31, 2001)
강희완 한경대학교 2003 論文集 Vol.35 No.-
Polymerase chain reaction (PCR) technology has been applied for various research fields of molecular biology. The PCR method requires little biological material and provides a rapid method for screening large sample sizes. PCR methods using ribosomal DNA and DNA polymorphism have been extensively used in assessing microbial diversity within or between species. PCR polymorphic methods have been developed using either arbitrary primers such as RAPD, AP-PCR and AFLP or specifically designed primers from known DNA information of repetitive sequences such as REP, ERIC and BOX. Rescently, URP primers of 20-mer were randomly designed from the repetitive sequence of rice. URP-PCR reaction can be perfomed under a stringent condition with a high annealing temperature of more than 55℃ and thus cause to improve PCR reproducibility. Therefore, URP primers amplified reproducible PCR polymorphism in microbial species at intra-and inter species levels.
강국희,이인선,박완수 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.2
The ferrnetnation characteristics and microbial changes during the storage of salted Chinese cabbage cultivars were investigated. When 10 Chinese cabbage cultivars produced in fall were salted up to 3 and stored at 0%, their pHs were lowered with different modes. and total acid contents were increased with the same way as pH. Results on microbial counts was the best in "noranmat'.
鄭玩鎬,車喜英,姜錫本 한국교원대학교 과학교육연구소 1993 청람과학교육연구논총 Vol.3 No.1
The major purpose of this research was to identify high school students' misconceptions on physiology. Misconceptions of scientific concepts have been believed to be very persistent and difficult to be overcome through regular classroom activities. Therefore, to find out students' misconceptions and students' cognitive structure must be very important to design instructions as well as curriculum. In order to find out high school students' misconceptions on physiology, a open-ended form of questionnaire on the physiology of several biological areas was developed and sent to fifty-three high school students. Their responses were collected and analyzed. From the analysis of all the Korean text books, it was found that some concepts were not described correctly. At the same time, the terminology used in the text books was different from book to book. These incorrect presentations and inconsistent use of terminology would have been the source of students' misconceptions on biology. Although the pattern of misconceptions that Korean students have in the area of photosynthesis was generally similar to one by foreign students, the misconceptions arose from biological terminology such as excretion and elimination were found to be specifically developed in Korean students who use Korean language. Although there a few special misconceptions in fragmentary lower-level concepts, there were some misconceptions in comprehensive and fundamental higher-level concepts. This probably could have resulted from the fact that too many concepts were included in current Korean textbooks and the entrance examination items were only knowledge level which need only be memorized line by line. Therefore, the content and concepts should be very carefully chosen in the text books.
석유정제산업 공정과 공정장비에 기인한 휘발성 유해 대기오염물질(HAPs)의 배출량 산정기법
조완근,권기동,동종인,강경희 한국환경과학회 2004 한국환경과학회지 Vol.13 No.7
Petroleum refineries have been considered as an important emission source for atmospheric volatile hazardous air pollutants(HAPs). The emission source includes petroleum refinery processes and process equipment. The control strategy for volatile HAPs requires emission estimations of these pollutants. However, systematic methods of volatile HAPs emission from petroleum refineries have not yet been established. Accordingly, present study surveyed the estimation method of volatile HAPs emitted from the petroleum refinery processes and process equipment. The emission estimation methods for the petroleum refinery processes are applied for 11 petroleum refining facilities: fluidized catalytic cracking, thermal cracking, moving bed catalytic cracking, compressed engine, blowdown system, vacuum distilled column condensator, natural gas or distilled boiler, natural gas or distilled heater, oil boiler, oil heater and flare. Four emission estimation methods applied for the petroleum refinery process equipment are as follows: average emission factor approach, screening ranges approach, EPA correlation approach and unit-specific correlation approach. The process equipment for which emission factors are available are valves, pump seals, connectors, flanges and open-ended lines.
성인원외폐렴의 원인미생물에대한 전향적 다기관 연구 : 성인원외폐렴의 원인으로 세균의 역할을 중심으로
우준희,강재명,김양수,신완식,류진홍,최정현,김양리,정희진,어수택,박춘식,정문현,정기석,이찬주,류지소 대한감염학회 2001 감염 Vol.33 No.1
Background : Communite-acquired peumonia (CAP) is one of the leading causes of mortality and morbidity. Despite progress in diagnostic techniques and treatments, management of pneumonia remains challenging, because the precise etiology remains uncertain in as many as 49% of cases. The limitaions of identifying etiologic agents make it necessary to use empiric antibiotics in almost all patients, and furthermore emergence of antibiotic-resistant organisms pose difficulties to the selection of an empiric antibiotic regimen. For the optimal choice of empirical antibiotics, we should know the frequency of etiologic agents and antimicrobial resistance rates in the community. Methods : A prospective multicenter study of community-acquired pneumonia in Korea was carried out between May 1997 and April 2000. The microbiologic diagnosis was based on the results of sputum culture, blood culture and pleural culture. Results : Five hundred eighty eight cases of community-acquired peumonia in 562 patients admitted to the hospitals. The mean age was 59.9 with male predominance (58.3%), and 370 (63%) had underlyin gillness. The etiologic agents were identified in 38.3%, and the list of individual agents, in decreasing order, was Streprococcus pneumoniae (21.7%), Klebsiella pneumoniae (14.8%) Pseudomonas aeruginosa (9.8%), Staphylococcus aureus (9.5%), viridans group streptococci (5.7%), Enterobacter cloacae (4.2%), Hemophillus Influenza (3.8%), The rates of admission to the intensive care unit was 10.4%. The motality was 7.1%. Susceptible rates of S. pneumoniae to penicillin was 36.6% and showed multidrug resistant. Forty percents of S. aureus were methicillin-resistant S. aureus. K. penumoniae were susceptible to cephalosporin and quinolone. Conclusion : In Korea, S.pneumoniae is the most important agent causing community-acquired pneumonia. Susceptible rates of S. pneumoniae to penicillin was 36.6% and showed multidrug resistant. Gram negative bacteria such as K. pneumoniae, P. aeruginosa showed high incidence when compared with that of other countries. Most of them had underlying diseases including bronchiectasis and chronic obstructive pulmonary diseases. (Korean J Infect Dis 33:1∼7, 2001)