생약복합물이 흰쥐의 체내에서 항산화 및 인지개선활성에 미치는 영향

강진순,Kang, Jin-Soon 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.3

인지기능 활성을 가진 생약복합물을 선정하여 최적조성물을 조제한 다음, 생약복합물의 첨가비율을 달리하여 치매유도 동물모델에서 항산화 효과와 인지기능 개선을 확인한 결과는 다음과 같다. 1. 20일간 사육한 흰쥐 혈청의 GOT, GPT, creatinine 및 BUN 활성을 측정한 결과, 생약복합물을 투여한 군(3, 4군)이 정상군(1군)에 비해 GOT 농도는 감소하였고, GPT 농도와 Creatinine 농도는 유의적인 차이는 없었다. BUN 농도는 정상군(1군)에 비해 생약복합물을 투여한 군(3, 4군)이 유의적으로 감소하였다. 2. 혈액 중의 총 콜레스테롤과 HDL-cholesterol은 유의적인 차이가 없었으나, 중성지방은 전군에서 정상군(1군)에 비해 치매유발군인 2군이 낮았고, 생약복합물 투여군(3, 4군), 치매치료군(5군)은 가장 낮았으며, 이들 군 간에는 유의적인 차이는 없었다. 3. 혈액 SOD 활성은 정상군(1군), TMT 투여군(2군) 및 tacrine 투여군(5군) 간에는 유의적인 차이가 없었으나, 생약복합물 투여군(3, 4군)이 다소 높아 유의적인 차이를 나타내었다(p<0.05). MDA 함량은 정상군인 1군과 치매치료군인 5군이 치매유발군인 2군과 생약복합물 투여군(3, 4군)에 비해 유의적으로 높았으며, 2, 3, 4군 간에는 유의적인 차이가 없었다. 4. AChE 활성은 TMT로 치매를 유발한 2군이 가장 높았으며, 치매치료군(5군)은 정상군(1군)의 수준으로 가장 낮게 나타났다. 그리고 생약복합물을 투여한 3군, 4군에서는 유의적 차이는 없으나, TMT로 치매를 유발한 2군보다는 유의적으로 감소하였고(p<0.05), 치매치료군인 5군보다는 다소 높게 나타났다. 5. 단기기억능력을 시험하기 위한 Y-maze를 실시한 결과는 Y-maze의 각 arm을 통과한 총 횟수는 전군에서 유의적인 차이는 없었다. 교차행동력은 TMT 투여군인 2군이 가장 낮았으며, 치매치료제인 tacrine을 투여한 5군이 가장 높아 5군(tacrine 투여)은 TMT 투여군인 2군에 비해 약 125% 현저한 기억력 증가를 보였다. 이에 반해 생약복합물 투여한 2, 3군은 정상군과 유의적인 차이가 없었으나, TMT 투여군인 2군에 비해 각각 117.32%와 120.38% 수준으로 기억력 증가를 보였다. 장기기억능력을 시험하기 위한 수동회피실험의 결과, TMT 투여군(2군)은 전군에서 가장 낮았으나, 생약복합물 중, 고용량 투여군(3, 4군) 및 tacrine 투여군은 증가하였고, 특히 고용량(4군)에서 유의성 있는 증가를 나타내어(p<0.05) 정상군에 가깝게 회복시켜 주는 경향을 보여 주었다. 이상의 결과를 통하여 생약복합물은 치매 유발 동물모델의 항산화능의 저하를 억제하고, AChE 활성을 억제하여 ACh의 활성을 촉진하므로 장 단기 기억능력의 회복 및 개선효과를 확인할 수 있었다. 그러나 향후 생약복합물의 농도에 따른 첨가군을 확대하여 인지기능 개선에 가장 효과적인 최적농도를 도출하는 연구가 계속 진행되어야 할 것으로 사료된다. The purpose of this experiment was designed to investigate the effects of medicinal herbs (MH) extracts on dementia induced by trimethyltin chloride (TMT) in rats. Six-week-old male Sprague-Dawley rats were randomly divided into five groups; normal group (group 1), control group (group 2), MH extracts group (250, 500 mg/kg) (group 3, group 4) and positive control group (tacrine group, group 5). In the control group to induce dementia, a 2.5 mg/kg of TMT intraperitoneal injection was used for 14 days (1 per day) in the rats. In the MH extracts group 250 mg/kg and 500 mg/kg of MH extracts were medicated in an oral inoculation for 20 days (1 per day). After 30 minutes, a 2.5 mg/kg of TMT intraperitoneal injection, which causes dementia, was used for 14 days (1 per day). In the positive control group (Tacrine group) 10 mg/kg of Tacrine, the dementia treatment, was medicated in an oral inoculation. After 30 mintues, 1 mg/kg of TMT intraperitoneal injection, which causes dementia, was used for 14 days (1 per day). The present author observed the passive avoidance performance test, and memory ability test (Y maze test), the values of MDA, acetlycholinesterase (AchE) activity in the brain and antioxidant enzyme in serum. MH extracts significantly improved memory of AD model rats in the Y-maze test, and also significantly improved memory of AD model rats in the passive avoidance test. MH extracts significantly reduced AChE activity, and significantly increased the SOD level, but not catalase and MDA. From the results above, MH extracts is thought to be effective in the improvement of antioxidant enzymes and memory ability.

아마인유와 해바라기 종자유의 혼합급이가 고지혈증 흰쥐의 비장 지질의 지방산조성에 미치는 영향

강진순 진주여자전문대학 1995 論文集 Vol.18 No.-

This study was carried out to investigate the effect of the feeding mixture of linseed oil, rich in n-3 PUFA and the sunflower seed oil, rich in n-6 PUFA on the fatty acid composition in the dietary hyperlipidemic rats. Afterr Male Sprague Dawley rats were induced hyperlipidemia by feeding the diet containing lard, butter and cholesterol for 3 weeks, then they were fed with the diet containing lard 3.0% and butter 12.0% for control, the mixture in different proportion of both linseed oil and sunflower seed oil and antihyperlipidemic drugs for 2 weeks. Analysis of the lipid composition and the fatty acid composition of the spleen lipid showed following results. In the total fatty acid contents of spleen lipids, saturates was remarkedly more than monoenes and polyenes, while there was not significant different among total diet groups. Polyenes of the total fatty acid was not distributed C_(18:2), C_(18:3) on test lipid, but it was distributed on C_(20:4)(n-6), C_(20:5)(n-3), C_(22:6)(n-3) on spleen tissue feed hyperlpidemic rats. In the fatty acid composittion of phospholipid in spleen, polyene contents of group 1-4 feed test lipid were 5.5-7.3% but increased to 26.0-36.3% in spleen lipid of hyperlidemic rats. In the fatty acid composition of triglyceride, polyene contents of group 5-9 feed test liplld were 59.1-62.3% but decreased 17.0-22.4% in spleen lipid of hyperlippidemic rats, on the contrary, polyene contents of group 1-4 feed test lipid were 5.5-7.3% but increased to 11.5-20.3% in spleen lipid of hyperlipidemic rats. In the polyenes, TG content of group 5-9 feed mixed linseed oil and sunflower oil was getting higher than group 1-4 feed butter or olive oil and it seems to be affected by the fatty acid composition of the test lipids. In the fatty acid composition of CE were remarkedly significant different among monoenes, saturates and polyenes in test lipid but it was different tendency in spleen lipid of hyperlippidemic rats. C_(20:5)(n-3) was the major fatty acid in total fatty acid and PL, while C_(18:2) (n-6) was the major fatty acid in TG and CE of spleen lipid. Each lipid composition was comparatively influenced test lipid rather than cholestyramine and antihyperlipidemic dose. Sunflower seed oil and linseed oil were effective for the improvement of spleen lipids.

아마인유와 해바라기종자유의 혼합급이가 고지혈증 흰쥐의 신장지질의 지방산조성에 미치는 영향

강진순 진주여자전문대학 1994 論文集 Vol.17 No.-

This study was carried out to investigate the effect of feeding the mixture of linseed oil, rich in n-3 PUFA and the sunflower seed oil, rich in n-6 PUFA on the fatty acid composition in the dietary hyperlipidemic rats. After Male Sprague Dawley rats were induced hyperlipidemia by feeding the diet containing lard, butter and cholesterol for 3 weeks, then they were fed with the diet containing lard 3.0% and sunflower seed oil and antihyperlipidenmic drugs for 2 weeks. Analysis of the lipid and the fatty acid composition of the kidney lipid showed following results. In the total fatty acid contents of kidney lipids, monoenes were remarkedly lower than saturates and polyenes, while there were not significantly different among total diet groups. As compare to fatty acid contents of test lipid feed hyperlipidemic rats, Saturates and polyenes in the total fatty acid contents were increased, on the contrary, monoenes were getting significantly decreased In the fatty acid composition of phospholipid in kidney, polyenes contents were the most among all of them, especially C_(20:5)(n-5) was the major fatty acid. In the fatty acid composition of TG and cholestrryl ester, monoenes and saturates content were similar, but polyenes content were remarkedly low as 7.0-25.8% since C_(20:0), C_(22:5)(n-6), C_(22:5)(n-6), C_(22:6)(n-3) were not distributed Polyenes contents of group 5-9 feed mixed linseed oil and sunflower oil were getting higher than group 1-4 feed butter or olive oil in the TG and cholesteryl ester, and it seems to be affected by the fatty acid composition of the test lipids. C_(20:5)(n-3) was the major fatty acid and PL, while C_(18:2)(n-6) was the major fatty acid in TG and cholesteryl ester of kidney lipid Each lipid composition was comparatively influenced by test lipid rather than cholestyramine and antihyperlipidemic dose. Sunflower seed oil and linseed oil were effective for the improvement of kidney lipids.

타부처와의 벅찬 싸움에서 밀리는 환경처 - 환경처위상 재정립은 요원한가

강진순 한국환경기술인연합회 1991 환경기술인 Vol.58 No.-

Vitamin C가 방사선과 Aflatoxin B1을 투여한 흰쥐 간세포의 항산화효소 및 Stress Protein에 미치는 영향

강진순 한국식품영양학회 2015 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2015 No.12

Vitamin C가 방사선과 Aflatoxin B1을 투여한 흰쥐 혈청 중의 항산화효소 및 혈액성분에 미치는 영향

강진순 한국식품영양학회 2015 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2015 No.12

`환경`태풍 극복할 방안은$\cdots$ - CFC 대체물질개발, 산업구조개편 서둘러야 한다 -

강진순 한국환경기술인연합회 1992 환경기술인 Vol.71 No.-

Vitamin C가 방사선과 Aflatoxin B₁을 투여한 흰쥐의 혈청과 간장의 지질성분 및 지방산 조성에 미치는 영향

강진순,김소영,김희숙,조흥래,채규영,정덕화,Kang, Jin-Soon,Kim, So-Young,Kim, Hee-Suk,Cho, Heung-Lae,Chai, Gyu-Young,Chung, Duck-Hwa 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.2

Aflatoxin은 Aspergillus속 곰팡이로부터 생성되며 사람에게 있어서 간독성 및 간암을 유발하는 잠재력을 가진 곰팡이 독소이며, 지질과산화 반응은 aflatoxin $B_1$에 의한 세포 산화적 손상 시 발생하는 주요 현상 중의 하나이다. 방사선은 수술, 항암약물요법과 더불어 임상 시 중요한 치료방법이나, 정상세포에 방사선을 조사하였을 때 반응성이 높은 활성산소와 과산화라디칼$(OH{\cdot})$을 생성하여 세포막의 불포화지방산을 지질과산화물로 변성시켜 세포 산화적 손상을 일으킨다. 따라서 본 연구의 목적은 aflatoxin $B_1$과 방사선조사 후 유도된 간과 혈청의 지질성분 및 지방산 조성에 미치는 vitamin C의 영향을 조사하기 위해 수행되었다. X-ray 조사는 실험기간 내 단 1회로 실험사육기간 첫 일에 조사하였고 X-ray 조사 후 vitamin C를 투여하였으며 vitamin C 투여 1시간 후 $AFB_1$을 투여하였다. Vitamin C와 $AFB_1$은 모두 복강투여로 실험 사육 첫 일부터 1회 시작하여 3일에 한번씩, 5회 반복 투였으며 실험동물 사육기간은 총 15일로 하였다. 혈청 총콜레스테롤 농도는 $1\sim5$군에서 유의적인 차이를 볼 수 없었는데 방사선과 $AFB_1$을 함께 처리한 6군에서는 $130.37{\pm}9.14mg/dL$로서 전군에서 가장 높은 수치를 나타낸 반면 6군에 vitamin C를 혼합 투여한 7군에서는 $90.23{\pm}6.15mg/dL$로서 가장 낮았으며 이는 중성지질과 유사한 경향이었다. 또한 간장의 총콜레스테롤과 유리 콜레스테롤도 이와 유사한 경향이었다. HDL 농도는 방사선과 $AFB_1$을 함께 처리한 것에 vitamin C를 혼합 투여한 7군이 $59.57{\pm}9.56mg/dL$로서 p<0.01 수준에서 현저히 증가하였다. 혈청의 인지질과 콜레스테롤 에스테르의 지방산 조성에서 vitamin C를 혼합 투여한 3,5,7군에서 PUFA 함량이 증가한 반면, SFA 함량은 감소하여 P/S 비율, n-3P/n-6P 비율은 증가하는 경향이었으며 이는 간장의 인지질, 콜레스테롤 에스테르, 총 지질의 지방산조성에서도 같은 경향을 볼 수 있었다. Lipid peroxidation is one of the main manifestations of oxidative damage and has been found to play an important role in the toxicity and carcinogenesis of many carcinogens. This study was carried out to determine the effects of vitamin C on lipid contents and fatty acid compositions of serum and liver in male rats treated with radiation or aflatoxin $B_1\;(AFB_1)$. Six week-old male Sprague-Dawley rats were randomly divided into 7 groups; control group, radiation exposed group, $AFB_1$ treated group, X-ray and $AFB_1$ co-treated group. Three groups, except control group, were each further divided into vitamin C administered group and not administered groups. For this study, vitamin C was injected with 10 mg/kg of body weight by intraperitoneal injection and 1 hr later, 0.4 mg/kg of $AFB_1$ was injected by the same method. These administrations were repeated every 3 days over a period of 15 days. Only one time, X-ray was irradiated on whole liver with 1,500 cGy. Then vitamin C and AFB1 were administered by the same level and same method described above. On the 16th day of treatments, the animals were sacrificed. From the analysis of the serum lipid patterns, significant decrease (p<0.01) in triglyceride (TG) and total cholesterol levels were observed in X-ray and $AFB_1$ co treated group administered with vitamin C (group 7). In liver lipids, the levels of free cholesterol and total cholesterol were also decreased in X-ray and $AFB_1$ co treated group administered with vitamin C (group 7). The levels of serum free cholesterol and hepatic TG were not significantly different among all groups according to vitamin C administrations. The high density lipoprotein (HDL)-cholesterol level of serum was significantly (p<0.01) increased while the low density lipoprotein (LDL)-cholesterol level was decreased in X-ray and $AFB_1$ co treated group administered with vitamin C (group 7). In the phospholipid fatty-acid compositions of serum and liver tissue, group 3, 5 and 7 showed an increase in polyunsaturated fatty-acid (PUFA) but a decrease in saturated fatty acid (SFA) when compared to the control group. The composition ratio of fatty acid varied according to vitamin C administration. These results suggested that vitamin C has partly suppressive effects on lipid contents and fatty acid composition of serum and liver in rats treated by radiation and $AFB_1$.

Aspergillus flavus , Aspergillaus niger 및 Peniciilum griseofulvum 의 혼합배양이 aflatoxin 및 patulin 생성에 미치는 영향

강진순,강성조,정덕화 한국식품위생안전성학회 2001 한국식품위생안전성학회지 Vol.16 No.3

This experiment was conducted to investigate the effects of mixed culture with mycotoxigenic and non-mycotoxigenic fungi on mycotoxin production. For this work, Aspergillus flavus (aflatoxin producing strain), Aspergillus niger (non-mycotoxigenic strain) and Penicilhum griseofulvum (patulin producing strainvere cultured in 5 ml SLS medium for 15 days under single or mixed culture. Affatoxin was determined by direct competitive ELISA, whereas, patulin was measured by HPLC. The mycelial growth, pH and total acidity were also observed by general methods. The mycelial growth was slightly decreased in the mixed culture, meanwhile total acidity was increased and pH was shown lower than that in single culture. Aspergillus flavus produced 145 ㎍/ml of aflatoxin for 12 days single culture, but in mixed culture, aflatoxin was decreased to 93%, and was shown as 10.16 ㎍/ml level. Patulin production in mixed culture was also decreased to 69.3% and was shown only 23.72 ㎍/ml level as compared with in single culture.

Four New Species of Symphypleona (Collembola) from Korea

강진순,이병훈,박경화 한국곤충학회 2005 Entomological Research Vol.35 No.4

Four new species of Symphypleona, Ptenothrix plumiseta, Deuterosminthurus serratus, Sminthurus annulatus, Sminthurinus distinctus, are described from Korea. Especially two genera, Deuterosminthurus and Sminthurinus are new record to Korea. Korean Collembola Symphypleona add up to 18 species, 11 genera in 6 family.