Collagen으로 유발된 생쥐의 관절염에 대한 당귀사역가오수유생강탕(當歸四逆加吳茱萸生薑湯)의 효과

김민균 ( Min Kyun Kim ),오민석 ( Min Seok Oh ) 한방재활의학과학회 2011 한방재활의학과학회지 Vol.21 No.2

Objectives: This study was carried out to know the effects of Danggwisayeokgaohsuyusaenggang-tang(hereinafter referred to DST) on arthritis induced by collagen on DBA/1 OlaHsd mice. Methods: For this purpose, DST was orally administered to mouse with arthritis induced by collagen Ⅱ. Cytotoxicity, high performance liquid chromatograph(HPLC) analysis, arthritis index, value of immunocyte in draining lymph node and paw joint, cytokine were measured in vivo. Results: 1. The cytotoxicity against human fibroblast cells(hFCs) was not measured in any concentration. 2. In HPLC analysis, There are high peak patterns at 8 minute(min), 12 min, 35 min, 45 min. 3. The arthritis index was decreased significantly. 4. The degree of arthritis induced damage of joint of DST group is slight compared with control group in histopathologic observation(Hematoxylin and eosin stain(H&E), Masson`s trichrome(M-T) staining). 5. In total cell counts of draining lymph node(DLN) and paw joint, the cells in DLN decreased significantly on DST 200 mg/kg and the cells in paw joint decreased significantly on 200 mg/kg and 50 mg/kg. 6. In DLN, CD4+/CD25+, CD3+/CD69+, major histocompatibility complex(MHC) class-II/CD11c+ cells decreased significantly on DST 200 mg/kg and 50 mg/kg, CD3+/CD8+ cells decreased significantly on DST 200 mg/kg, CD4+, CD3+/CD44+ cells decreased. 7. In paw joints, CD4+, CD11b+/Gr-1+ cells decreased significantly on DST 200 mg/kg and 50 mg/kg. 8. In joints, levels of IL-1β, IL-6, TNF-α, cyclo-oxygenase-2(COX-2), NOS-II were decreased on DST 200 mg/kg and DST 50 mg/kg. 9. In analysing of cytokine in CD3/CD28 activated spleen, IL-17 was decreased significantly, IL-4 was increased significantly and INF-γ was decreased on DST 200 mg/kg. 10. In analysing of cytokine in collagen activated spleen, IL-17 were decreased significantly, IL-4 was increased significantly. Conclusions :This results demonstrated that DST suppressed the inflammatory progression of collagen-induced arthritis(CIA) mice and supported further studies are required to survey continuously in looking for the effective substance and mechanism in the future.

도시민의 여가 선용을 위한 공간 확충 방안

민춘기,오노균 忠南大學校體育科學硏究所 1993 體育科學硏究誌 Vol.11 No.1

This research was tried to find out the expnasion methods of leisure space for citizen rest. The objects of study was composed of 40 public official servants and 238 citizen whose have selected by random sampling. The following were the conclusion. 1. A concept of leisure space surveyed in order of replied that they wanted comfortable place which break away from pollution(noise, environment air), gave in pleasure and relieve the fension, gave in recreation and activity of life. 2. They call on the his favorite place inside of 1km: Bomun M. T. park, Mini-park of downtown, river side park, fitness park in village but find out a few park of leisure. 3. The rent of leisure space surveyed replied that, They wanted to free pass and make use of 3-4 day on weeks with his family and follows. 4. The expansion of leisure space and physical fitness park surveyed replied that they wanted become of the main group according to self-governing body. 5. The problem of leisure space for expansion finded out financial affairs and purchanse of the ground. They insisted on that these problems must supporting both a self-governing body of localiy and government. 6. A scale of leisure park find out favorite Mini-park and Middle park which adequate to Korea's leisure siture situation.

한국인에서 혈액응고인자 IX 유전자의 제한효소 절편길이 다형성에 관한 연구

전봉균,이풍연,권오병,이정민,안주미,권무식 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

DNA-based prenatal diagnosis or carrier detection for hemophilia B in Korean has been developed by RFLPs. The polymorphisms were Taq I and Xmn I . Genomic DNAs were extracted from the blood of 56 females and 10 males at the age of twenty. The PCR primers were chemically synthesized by the method of phosphoramidite. Taq I-59 & -39 derived from flanking sequences of intron 4 generate 163nt fragment. And, Xmn 1-59 & -39, derived from the flanking sequences of intron 3 allow amplification 222nt fragment. Using Polymerase chain reaction(PCR) method, each segment(intron 3-Xmn I, intron 4-Taq I) was amplified with each primers and the genomic DNA as template. The amplified DNA fragments were treated with an appropriate restriction enzyme. The Xmn I polymorphism of the factor IX gene was detected on agarose gel as segments of 154 and 68nt, respectively. The heterozygote frequency, calculated from the allele frequencies (0.025/0.975) in intron 3-Xmn I polymorphism, was 4.8%. In case of intron 4-Taq I polymorphism, there is no polymorphic site. So that the heterozygote frequency calculated from the allele frequencies (0.0/1.0) in intron 4-Taq I polymorphism, was 0%. Thus, the two intragenic polymorphisms predicted to be informative was 4.8% in this study. The results are not correlated with those obtained from the Caucasian. It suggests that Korean exhibit different patterns of Xmn I and Taq I polymorphism in the human coagulation factor IX gene.

배추(Brassica campestris L. var. pekinensis Makino)의 cDNA library 구축 및 상동성 비교

안주미,권오병,전봉균,이풍연,이정민,권무식 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

Expressed sequence tag(EST) has a good value to discover a new gene or to study its structure. Some 20 ESTs were generated to obtain new genetic resources of chinese cabbage(Brassian campestris L. var. pekinensis Makino). Poly A+ RNAs were isolated from 10-day-old seedlings grown at 25℃ under the day light. cDNA gene bank was constructed using λ ZAP /cDNA synthesis /Giga Pack Gold Packing kit. About a million clones were able to obtain from the library. All the clones examined so far had insert DNAs. Nucleotide compositions of randomly selected clones were determined by the Sanger mthod. The DNA sequences were compared with those deposited in the GenePept and GenBank database to figure out nucleotide homologies. Two ESTs showed significant similarities to the enlisted sequences. They are chloroplast GADPH subunit of Arabidopsis thaliana and carbonic anhydrase of A. thaliana. The full DNA sequences of the two clones are being determined. The cDNA gene bank constructed. in this experiment will being used to isolate more genes induced by the light in the plant.

韓國人에서 血友病 B 遺傳子(血液凝固因子 Ⅸ)의 構造 및 制限酵素 Dde Ⅰ, MnI Ⅰ 切片길이 多形性에 關한 分子 遺傳學的 考察

이풍연,전봉균,이정민,권오병,권무식 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.2 No.2

Hemophilia B, a human chromosome X-linked recessive disease, is a bleeding disorder resulting from defect or abnormality in blood coagulation factor IX. DNA-based prenatal diagnosis or carrier detection for hemophilia B in Korean has been developed by analyzing restriction fragment length polymorphisms(RFLPs). Two polymorphisms( Dde I and Mnl I) were investigated as follow. Genomic DNAs were extracted from blood of 50 females at the age of twenty. The primers were chemically synthesized by the method of phosphoamidite. Mnl I primers were derived from exon 6, while Dde I primers, from the flanking sequences of intron 1 of the factor IX gene. Genomic DNAs were amplified with Mnl I primers to generate 405 nts long fragments in all cases. They were digested with Mnl I to analyze the polymorphic site on agarose gel. No MnlI polymorphic site was found in all cases. Also, the genomic DNAs were amplified with Dde I primers to generate ca. 320 nts long fragments in all cases. These results are not correlated with those obtained from the Caucasian. It suggests that Korean could exhibit different patterns of Dde I and Mnl I polymorphisms in the gene for blood coagulation factor IX. Direct sequencing of the polymorpic sites will confirm the above sugestion.

항 혈소판 요법을 이용한 관동맥내 Stent 삽입술의 조기 결과

손지원,김영준,손민수,오세진,안태훈,최인석,신익균 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.6

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

심혈관계 질환으로 관상질환 집중치료실에 입원한 환자들의 혜후 측정을 위한 APACHEⅢ점수 체계의 임상적 유용성

이공섭,이창선,최현주,홍종서,강지향,김도형,이민영,오인균,김병엽,김상억,김학찬,한승혜,이재용,지영우,안경주 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

Transcutaneous Approach for the Removal of Parotid Gland Stones

Oh, Hee-Kyun,Park, Hong-Ju,Oh, Je-Seok,Jung, Seunggon,Kook, Min-Suk by Mutaz B. Habal, MD. 2014 JOURNAL OF CRANIOFACIAL SURGERY - Vol.25 No.6

ABSTRACT: Salivary stones in the parotid gland can be treated with a wide range of methods. Stones close to the opening of the duct can be easily removed through the oral cavity, whereas the entire salivary gland may need to be removed if stones are located close to the parotid gland. In such cases, surgical removal of the parotid gland may be lengthy and may produce facial nerve injury. We report a simple extraoral approach used for the removal of parotid gland stones located close to the parotid gland by precisely identifying the location of stones in 2 patients with parotid sialolithiasis.