뇌 동맥류를 동반한 Takayasu's Arteritis 1례 : 증례 보고 Case Report

이하우,홍순기,한용표,허철,김헌주 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.5

A Case of Takayasu's arteritis associated with intracranial aneurysm is described. We discussed the clinical features, radiologic findings of the case, and reviewed the literatures of this disease entity.

The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

Isolation and Genetic Characterization of Orientia tsutsugamushi from Scrub Typhus Patients in Gyeongsangnam-do, Korea

Ha, Jong-Hun,Lee, Dong-Hae,Park, Jin-Sik,Cho, Oh Hyun,Kim, Dong-Hoon,Ha, Na-Young,Cho, Nam-Hyuck,Hwang, Kyu Jam,Cho, Yong-Gon,Lee, Hye-Soo,Lee, Won Kil,Lee, Yoo Chul,Cho, Myung-Je,Lee, Woo-Kon,Shin, M The Korean Society for Microbiology 2016 Journal of Bacteriology and Virology Vol.46 No.4

Orientia tsutsugamushi (O. tsutsugamushi), which is endemic to an Asia-Pacific region, has increased its incidence and caused annually around 10 thousand patients infected with scrub typhus in Korea in the past several years. In the present study, we isolated 44 O. tsutsugamushi from the patients with febrile illness accompanied with or without an eschar in Gyeongsangnam-do, Korea. These isolates were characterized by genetic analysis of the major outer membrane protein, the 56-kDa type-specific antigen (tsa56), which is unique to O. tsutsugamushi. Two types of sequences of tsa56, designated by JJ1 and JJ2, were determined from 37 and 7 isolates of the 44 isolates, respectively. JJ1 and JJ2 showed 74.7~90.8% identity in nucleotide sequence and 66.1~90.5% identity in amino acid sequence with 33 reference strains except for Boryong and Kuroki. JJ1 and JJ2 had 100 and 99.9% nucleotide identity to Boryong strain, and 99.9 and 99.8 % to Kuroki, which has been known to be similar to Boryong, respectively. In addition, they showed 77.9~ 81.4% nucleotide identity with the cluster of Gilliam-related genotypes, whereas they showed higher nucleotide identity (89.6~90.8%) with the cluster of Karp-related genotypes. To our knowledge, this is the first report to isolate O. tsutsugamushi and characterize their genotype as the Boryong in Jinju and West Gyeongsangnam-do, Korea, even though it has been reported that the Boryong was the predominant genotype in isolates from chiggers, domestic rodents, and patients in the southern part of Korea. Furthermore, our isolates could be useful source to study on the pathophysiology and epidemiology of scrub typhus in Korea.

Isolation and Genetic Characterization of Orientia tsutsugamushi from Scrub Typhus Patients in Gyeongsangnam-do, Korea

Jong-Hun Ha,Dong-Hae Lee,Jin-Sik Park,Oh Hyun Cho,Dong-Hoon Kim,Na-Young Ha,Nam-Hyuck Cho,Kyu Jam Hwang,Yong-Gon Cho,Hye-Soo Lee,Won Kil Lee,Yoo Chul Lee,Myung-Je Cho,Woo-Kon Lee,Min-Kyoung Shin 대한바이러스학회 2016 Journal of Bacteriology and Virology Vol.46 No.4

Study on Bi-2212 Rutherford Cabling Process for SMES

Dong-Woo Ha,Sang-Cheol Kim,Il-Yong Han,Jae-Geun Oh,Jung-Hun Lee,Sang-Soo Oh,Hong-Soo Ha,Kyu-Jeong Song,Myung-Hwan Sohn,Rock-Kil Ko,Ho-Sup Kim,Tae-Hyung Kim IEEE 2008 IEEE transactions on applied superconductivity Vol.18 No.2

<P>The important merit of Bi-2212/Ag high T<SUB>c</SUB> superconducting wire is to apply cable as round wire geometry. Bi-2212/Ag wires were fabricated in order to develop Rutherford cable for SMES near the future. Various Ag ratio and various numbers of filaments for PIT (Powder-In-Tube) process were applied to investigate the workability during drawing and to improve critical current property of the wires. Rutherford cabling machine for B-2212/Ag strands was designed and manufactured. We designed and manufactured Rutherford cabling machine with 32 bobbins which had 2 km length of wire. This machine was constituted with Turks head, caterpillar tread and take-up facility. In order to obtain uniform working conditions without defects, Turks head's rolling system and Ag ratio of Bi-2212/Ag strands were modified. We tried to fabricate Rutherford cables with 20 and 30 Bi-2212 strands. Proper working conditions for without breakage strand in cable were investigated. We made successfully Bi-2212 Rutherford cable. The critical current of Bi-2212 Rutherford cable with 30 strands showed 420 A at 64 K and 4008 A at 4.2 K. The critical current of Bi-2212/Ag wire with 847 filaments was 398 A at 4.2 K and self field.</P>

Purification of Wastewater From Paper Factory by Superconducting Magnetic Separation

Dong-Woo Ha,Tae-Hyung Kim,Myung-Hwan Sohn,Jun-Mo Kwon,Seung-Kyu Baik,Rock-Kil Ko,Sang-Soo Oh,Hong-Soo Ha,Ho-Sup Kim,Young-Hun Kim,Tae-Wook Ha IEEE 2010 IEEE transactions on applied superconductivity Vol.20 No.3

<P>It is environmentally important to recycle the wastewater, since paper factories use a large amount of water and equivalent amount of wastewater is generated. Conventional water treatment facilities like precipitation process need large-scale equipment and wide space to purify the wastewater of paper factory. In case of massive waste water, high gradient magnetic separation (HGMS) parts are more effective to purify it rapidly and to occupy relatively small space, since large voids at filter with HGMS are adopted. In this respect, we prepared two types of superconducting magnets in order to apply HGMS parts for wastewater purification process. We made cryo-cooled Bi-2223 (HTS) superconducting magnet parts with room temperature bore in diameter of 70 mm and 200 mm in height. Also, cryo-cooled Nb-Ti superconducting magnet with room temperature bore in diameter of 100 mm and 600 mm in height was used for magnetic separator. Magnetic filters were designed by the analysis of magnetic field distribution at superconducting magnets. The faster wastewater's flow rate was, the worse the degree of clarity in treated water was. The ability of wastewater treatment in the case of magnetic separation used with 10 of magnetite particles was better than that in the case of 5 of magnetite particles.</P>

Optimization of Expression Conditions for Soluble Protein by Using a Robotic System of Multi-culture Vessels

( Woo Sung Ahn ),( Ji Young Ahn ),( Chan Hun Jung ),( Kwang Yeon Hwang ),( Eunice Eunkyeong Kim ),( Joon Kim ),( Ha Na Im ),( Jin Oh Kim ),( Myeong Hee Yu ),( Cheol Ju Lee ) 한국미생물생명공학회 2007 Journal of microbiology and biotechnology Vol.17 No.11

Prognostic Impact of Complete Pathologic Response Following Preoperative Transcatheter Arterial Chemoembolization for Hepatocellular Carcinoma in Liver Cirrhosis Patients Undergone Liver Resection or Transplantation

( Woo-hyeong Kang ),( Shin Hwang ),( Young-joo Lee ),( Ki-hun Kim ),( Chul-soo Ahn ),( Deok-bog Moon ),( Tae-yong Ha ),( Gi-won Song ),( Dong-hwan Jung ),( Gil-chun Park ),( Sung-gyu Lee ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Purpose: This study aimed to assess the prognostic impact of complete pathological response (CPR: tumor necrosis ≥ 99%) after preoperative transcatheter arterial chemoembolization (TACE) for hepatocellular carcinoma (HCC) on long-term survival outcomes in patients undergoing resection of liver resection (LR) or liver transplantation (LT). Methods: The clinical outcomes of patients showing CPR after LR (n=110) or LT (n=233) were analyzed, with each control group of minimal risk (solitary HCC ≤ 2cm, absence of neoadjuvant treatment and R0 resection for LR control (n=476); one or two HCCs ≤ 2cm and absence of neoadjuvant treatment for LT control (n=184). Results: In LR study group, 1-, 3- and 5 year tumor recurrence rates were 18.5%, 50.6% and 58.7% respectively, which are significantly higher than in RL control group (p=0.000); 1-, 3- and 5 year patient survival rates were 97.8%, 82.0% and 69.1% respectively, which are significantly lower than in RL control group (p=0.000). In LT study group, 1-, 3- and 5 year tumor recurrence rates were 4.1%, 7.9% and 7.9% respectively, which are significantly higher than in RT control group (p=0.019); 1-, 3- and 5 year patient survival rates were 92.7%, 89.2% and 86.9% respectively, which are not significantly lower than in RT control group (p=0.112). When comparing LR and LT study groups, tumor recurrence and patient survival curves showed significant differences (both p<0.000) (Fig. 1). The main site of tumor recurrence was intrahepatic recurrence in both LR study and control groups, and extrahepatic metastasis in both LT study and control groups. Conclusion: Patients achieving CPR after TACE followed by LT showed very favorable outcomes, which is comparable to those with minimal risk of recurrence. In contrast, following LR, down-staging effect from CPR was not definitely high and most tumors recurred at the remnant liver, thus strict surveillance is necessary as like in other HCC patients not showing CPR. These results would be reference data for studying the influence of widely variable degrees of pathological response after TACE in further clinical studies.