Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

청소년 도덕적 동기화 검사 도구 개정 연구(2차년도)

전종희 ( Jong Hee Jeon ),이인재 ( In Jae Lee ),김영한 ( Young Han Kim ),박균열 ( Gyun Yeol Park ),윤영돈 ( Young Don Yoon ),류숙희 ( Sook Hee Ryue ),홍성훈 ( Seong Hoon Hong ) 한국윤리교육학회 2013 윤리교육연구 Vol.0 No.29

본 연구는 J. Rest의 4구성요소 모형을 바탕으로 1차년도(2011년)에 개발된 도덕적 동기화 검사를 보다 신뢰롭고 타당한 도구로 개정하는 것이다. 따라서 선행연구를 보완하여 도덕 동기화의 구성 요소를 도덕지향성과 도덕적 정서귀인 등 두 항목으로 재설정하고, 스토리와 문항을 수정하였다. 개발된 세 개의 스토리(과제, 놀이공원, 작품) 별로 신뢰도를 분석한 결과, 전반적으로 .90 내외의 높고 수치를 보였다. 응답의 편포성을 완화하기 위해 2차년도에는 7점 척도를 사용하였다. 예비조사를 위해서는 전국 중학교 2학년생 409명을 대상으로 했고, 본조사를 위해서는 전국 6,216명의 중학교 2학년생을 대상으로 실시하였다. 개발된 검사도구의 신뢰도계수(문항 내적 일치도인 크론바흐 알파)는 .90이상으로 신뢰할만했다. 타당도를 검증하기 위하여 요인분석을 실시한 결과, 과제 스토리와 놀이공원 스토리의 일부 문항이 가정한 요인으로 잘 묶이지 않았는데 이 문항들을 제거하고 요인분석을 다시 실시해본 결과 기대하는 바를 충족했다. 그리하여 2차년도 연구를 통해 최종 스토리는 총 세 개(과제, 놀이공원, 작품)로 확정되었고, 각 스토리별 문항수는 과제 9개 문항, 놀이공원 9개 문항, 작품 10개 문항으로 총 28개 문항으로 확정되었다. 본 연구를 통해 수정 확정된 도덕적 동기화 검사도구는 상당히 안정적인 신뢰도와 타당도를 확보하였기에, 향후 우리나라 청소년의 도덕적 동기화 능력을 측정하기 위한 검사도구로 활용되기에 적절할 것으로 판단된다. The study aims to revise Moral Motivation Test I which was set for adolescents based on Rest`s four component model in 2011 and make standardized final morality test. In theory, this study is based on 4 components theory of James Rest who invented DIT (Defining Issues Test) to measure the moral judgment. The 4 components are composed of moral sensitivity, moral judgment, moral motivation, and moral character (or moral implementation, moral action). This study focused on moral motivation. This 2nd year study could get some significant outcomes. To make test form, 3 components relating teams have closely exchanged informations. Because teams have to share same real life stories as follows: “homework inspect”, “on going to park”, “broken art work”. The questionnaire used seven likert type to lessen the far epistemological tendency. To represent moral motivation, research team used moral orientation, moral emotion attributions. To get final form of morality test, this study was made two sequential steps. First one was pre-test which was for 409 respondents. Second main one was for 6,216 respondents. For moral motivation, the reliability was highly coefficient with Cronbach`s alpha(>.90). The validity was also much improved and stable. But just in the story “homework inspect”and “on going to park”, the factor analysis shows a little bit limitation result. Eliminated those questions, the result of factor analysis was more stable and reliable. Through these processes, to test moral motivation, this study shows 3 stories and 28 questions. And this recommends that just one simple story might be developed for one component(moral motivation).

한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률

조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

경피적 승모판막 성형술 전후 측정방법에 따른 승모판구 면적

성인환,이종구,박종훈 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

To assess mitral valve area(MVA) before and after percutaneous mitral ballon valvuloplasty (PMV), MVA was calculated by 2-D and Doppler (pressure half time) echocardiography before and 1 day after PMV. Also the catheterization derived MVA was calculated by modified Gorlin's fomula before and immediately after PMV. Among a total of 63 patients performed successful PMV, 29 patients were female and 24 patients were male. The age of the patients were between 21 and 68 years with the mean age of 42±12 years. On the EKG 34 patients showed a sinus rhythm 29 patients showed an atrial fibrillation. PMV was performed using Inoue balloon in 31 patients and double balloon in 32 patients with mitral stenosis. After PMV MVA by Gorlins formula, 2-D and Doppler echocardiography was increased from 0.84±0.22㎠ to 1.86±0.47, 1.86±0.31 and 1.76±0.34㎠, respectively (p<0.01). Before PMV MVA determined by the Gorlin's formula correlated closely with MVA by 2D and Doppler echocardiography (r=0.66 and r=0.69 respectively). However, after PMV MVA determined by the Gorlin's formula did not correlate well with MVA by 2-D and Dopper echocardiography (r=0.32 and r=0.41) However, after PMV MVA determined by the Gorlin's formula did not correlate well with MVA by 2-D and Doppler echocardiography (r=0.32 and r=0.41 respectively). Then Correlation between the 2-D planimeteral MVA and doppler derived MVA was good (r=0.82) After PMV the discrepancy of correlation may be bue to lack of simultaneous meusurements of MVA by echocardiogrphy and catheterization. Immediately after PMV the dramatic hemodynamic changes by have a disturbing effect of MVA measurement by Gorlin's formula. In conclusion, 2-D and Doppler echocardiography was non-invasive and effective method for assessment of MVA before and after PMV.

Protein microarray를 이용한 Apin-단백질의 상호작용에 관한 연구

박주철,박선화,김흥중,박종태,윤성호,김지웅,이태연,손호현 대한치과보존학회 2007 Restorative Dentistry & Endodontics Vol.32 No.5

전기장 인가에 따른 DNA 신장에 관한 AFM 연구

박진영,한성웅,손세영,송성훈,김종민,Ohtani, Toshio,Muramatsu, Hiroshi,장상목 한국화학공학회 2002 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.40 No.1

본 연구에서는 마이카 표면상에서 전기장에 의한 극히 낮은 농도의 DNA 신장에 대한 효율적인 방법론을 제시하였다. 전기장에 의한 DNA 신장을 AFM형상으로 분석한 결과, DNA 농도범위가 약 57×10 exp(-3)ng/mL-57×10 exp(-6)ng/mL일 때 매우 잘 적용되며, 신장된 DNA는 두 전극 중앙에 존재하였다. 또한, 일반 마이카, Mg^2+ 처리된 마이카, 그리고 AP-마이카 표면들에 대한 신장 효율의 차를 기술하였다. 동일한 실험조건 하에서 AP-마이카의 표면이 가장 좋은 효율의 DNA 신장 결과를 나타내었다. An effective method of DNA stretching using electric field on mica surface was proposed for extremely low solution concentration of DNA. DNA stretching based on electric field was observed by AFM and well applied on the concentration range from 57×10 exp(-3)ng/mL to 57×10 exp(-6)ng/mL. Stretched DNA existed in the middle of two electrodes. The difference in stretching efficiency for the different surfaces of bare mica, Mg^2+ soaked mica and AP-mica was discussed. The best performance of stretching was found from the surface of AP-mica under the same experimental condition.

善神과 惡神의 相互模做과 協演의 창조론과 善惡의 문제

박종성 한국방송통신대학교 통합인문학연구소 2009 통합인문학연구 Vol.1 No.2

창세의 주역 신들 사이에 능력의 우열관계가 확정된 경우와 그렇지 않은 경우,그리고 두 주역 신 사이에 협연의 창조 행위와 모방의 창조 행위, 그리고 대립과 갈등을 통한 창조 행위 등은 창세 신화의 다채로운 양상을 형 성하는 중요한 자질이 된다. 이 논문에서는 동유럽, 한국, 몽골의 자료들을 대상으로 ‘거짓 잠자기’ 와 같은 방식을 활용하여 능력의 우열관계를 지속시키는 경우와 역전시키는 경우, 그리고 선과 악의 분별이 확정적인 경우와 이에 관한 판단을 유보 한 채로 전승을 거듭하는 경우 등을 고찰함으로써 신화적 사유의 방식과 함축적 의미를 파악할 수 있는 근거를 마련하였다. Genesis myths have various aspects depend on the following contents of the text : when comparative merits and demerits between two principal gods of genesis is established, or not ; collaboration and mutual imitation of the each side of the principal gods is dominated ; confrontation and disagreement between Good and Evil is emphasized. In this paper, I selected cosmogonic myths of Eastern Europe, Korea and Mongol as research materials in order to examine various aspects of Genesis myths. I examined cases that superiority or inferiority between two principal gods continues or goes into reverse due to practice “A False Sleeping” or similar acts of one of the side. Also, I examined cases that distinction between Good and Evil is established or cases that discern Good from Evil is not judged. This paper would help you to understand mythical thinking and profound implications of myths.

15세기 上院寺 立案文書 분석

朴盛鍾,朴道植 한국고문서학회 2002 古文書硏究 Vol.21 No.-

본고는 15세기 후반에 上院寺에 발급되었던 세 점의 立案 문서에 대해 검토해 보았다. 강릉대도호부에서 상원사에 발급한 입안이 두 點, 內需司에서 상원사에 발급한 것이 한 點이다. 대상 입안 문서에 대하여 원문을 교감·판독하였고, 이를 토대로 史讀字들에게는 밑줄을 긋고 당대의 讀音을 추정하였으며, 더 나아가 문서 내용을 현대어로 번역해 보았다. 그리고, 實錄의 기사 등을 참고로 하여 상원사를 중심으로 조선 초기 왕실관련 사원의 경제적 면모를 살펴보았다. 1469년(예종 1)에 강릉대도호부에서 발급한 입안은 蒜山提堰을 상원사에 題給함과 동시에 鹽盆稅와 諸雜役을 減除하는 것이고, 두 번째 1477년(성종 8) 강릉대도호부 입안은 예종의 願刹인 낙산사와 세조의 원찰인 상원사의 分屬과 持戒僧人에 의해 대를 이어 持守할 것을 전한 것이다. 세 번째 1481년(성종 12) 內需司 입안 내용은 낙산사에서 거두어들이는 布物·綿花를 상원사에 半分하여 사용하라는 것이다. 조선 초기 왕실관련 사원에는 收租地와 노비가 지급되었다. 상원사는 수조지 외에도 私有地도 소유하고 있다. 그것은 세조가 상원사에 거주하고 있었던 信眉에게 蒜山堤堰을 賜給한 것에서 알 수 있다. 實錄에 따르면 예종 원년에 이 蒜山堤堰을 다시 사급하였다고 하였는데, 이는 첫 번째 문서에서 그 사실이 확인된다. 상원사에 대한 특혜는 田地에 국한되지 않고, 내수사 노비를 特賜하기도 하고, 각종 雜役과 요역, 사원 소유의 鹽盆稅를 면제해 주기도 하였다. 이 사실 역시 첫 번째 문서와 두 번째 문서에서 확인된다. 睿宗의 願刹인 낙산사와 世祖의 願刹인 상원사의 관계 및 田稅貢物의 半分 사용 등에 관한 내용도 두 번째 및 세 번째 문서에서 드러난다. The aim of this thesis is briefly to study the three Iban documents of the Buddhist temple Sangwonsa, which is located in Pyungchang(平昌) of Gangwondo(江原道). Those documents were written in Idu(史讀) in the latter half of the 15C. In this thesis, they were translated in Modern Korean, and Idu scripts were as well underlined and read according to the presumed pronunciation. The first and the second document of those threes were respectively in 1469 and 1477 by the provincial government Gangneung-daedohobu(江陵大都護府), and the third was made by Naesusa(內需司) in 1481. The purpose of the first document was to donate the land Sansanjeeon(蒜山堤堰) to Sangwonsa and to make Sangwonsa be free of various taxes and obligatory labors. In the second and the third document, the relationship of the two Buddhist temples, Sangwonsa and Naksansa(洛山寺), was mentioned. The relationship between the Sangwonsa and royal household began when King Taejo designated it as his Wondchal(願刹, prayer temples). In the early Chosun Dynasty, the Taxation Article and slaves were given to the temples which had relations with royal household. In the King Taejong era, when the temples and their slaves were forfeited, the Sangwonsa was exempt from forfeit. Thereafter also in the 6th year of King Sejong, only Gwan-eum-gul(觀音窟) and Jin-gwan-sa(津寬寺) were designated Su-ryuk-sa(水陸社) as well as Sangwonsa and Gyun-am-sa(見庵寺). Some time later the Taxation Article of Gyung-bok-sa(景福寺) in Jeonju was included in Sangwonsa. The temple land and Guk-haeng-su-ryuk-jeon(國行水陸田) were given as the Taxation Article of Sangwonsa. The Sangwonsa was possessed the private land besides the land of Taxation Article(收租地). It got to know from the fact that the King Sejo gave Buddhist priest Sinmi(信眉) long-time uncultivated San-San-je-eon(蒜山堤堰) one year after a Buddhist lecture meeting of regeneration and completion of a building in Sangwonsa. But it was written that in the first year of King Yejong the San-San-je-eon was given to the Sangwonsa again. The royal gift in the King Yejong period was understood as the reconfirmation of the gift in the King Sejo period. After that Sinmi gave the San-San-je-eon to his disciple Hakyeol(學說), who used to take away Minjeon(民田, people's land) around the je-eon claiming it as a reclaimed land. Special privilege to the Sangwonsa was not limited only to land. Naesusa(內需司) slavery privillege, various kinds of public works(諸雜役), labor service(요역) and salt tax(鹽盆稅) belonging to the temple were exempted. Labor service in early Chousun Dynasty was thoroughly imposed according to the land, but most of the temples related to royal household including Sangwonsa were exempted from labor service by the king. Exemption of labor service was the critical reason of increasing the temple's private lands. Because of this at the time Sangwonsa was widely taking possession of lands, also in spite of prohibition of giving lands to temple, the temple's private lands increased a lot. Sangwonsa was operated by Jang-ri(長利, an annul interest of fifty percent) and by Bangnap(防納, payment by proxy) behavior. Hakyeol made provincial governors to distribute cotton cloth(綿布) to the public compulsorily. The Public was charged 2seok(石) 5doo(斗) for 1pil(匹) of cotton cloth. Those who could not pay back within the due data were charged interest. This interest imposed by Hakyeol was a kind of Beondong(反同), taking high interest after lending rice and cloth to the public compulsorily since the Koryo Dynasty. Naksansa was Wonchal builded for luck with money of son(later king Yejong). After King Sejo designated Naksansa as his son's Wonchal, he gave rice and cotton cloth as almgiving, and he allowed monks to cultivate 30seok fertile lands near Naksan. During King SeongJong's reign, he granted 200kyeol the Taxation Article of Sam-ga-jeon(三嘉田), Eu Eui-ryeong-jeon(宜令田) and Cheong-do-jeon(淸道田) in Gyeongsang Province to the Naksansa. while monks in Naksansa were wealthy enough by selling cottons collected as a tributary payment every year, those in Sangwonsa were always lack of general expense because Sangwonsa received the Gangneung land that could not produce cottons. So King SeongJong ordered Naesusa to share half of cottons from Naksansa with Sangwonsa forever, which was evident in the third of Sangwonsa's Iban documents. The privileges for Naksansa was not only land but also rice. King SeongJong gave 100seok of Gyeong-Chang-mi(京倉米) every year and granted Sa-Pae(賜牌, royal Certification) which allowed Naksansa to have slaves forever. Moreover Naksansa had exclusive right to fish within 4Km of its fore seaside and prohibited the public to enter that area. Naksansa made slaves to gather sea products and fish, and it piled up its wealth by monompolizing profits from selling those products.