Thioacetamide에 의한 BALB/c 마우스 간의 시간별 약물대사효소 억제 양상 : A Time-Course Study

이정운,고우석,김갑호,배연경,하현정,한상섭,천영진,정태천 영남대학교 약품개발연구소 2001 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-

Thioacetamide is a potent hepatotoxicant which requires metabolic activation by cytochrome P450s (P450s) for toxicity. In the present study, the elevation kinetic of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities by thioacetamide treatment was investigated in male BALB/c mice. Inaddition, the inhibitory effects of thioacetamide on liver microsomal P450 enzymes were further investigated. Thioacetamide at 100 mg/kg/ was treated intraperitoneally for 6, 12, 24, 36, 48 and 72 hr. The blood was collected at the designated time for assaying the serum enzyme activities. To determine the P450 isozyme-specific activities. ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and benzyloxyresorufin O-debenzylase (BROD) activities were determined for P450 1A1, 1A2 and 2B1, respectively, in liver microsomal fractions. The activities of ALT and AST were started to be elevated 6 hr after thioacetamide treatment andreached the maximun at 36 hr after the treatment. The elevated activities were dramatically recovered at 72 hr. The microscopic exmination of the liver specimen also showed a similar profile of hepatotoxicity. All P450-associated enzyme activities were time-dependently inhibited by the treatiment with thioacetamide. The maximum inhibition of P450 enzymes was observed 36 hr after the treatment. Because the inhibition of P450 enzymes by thioacetamide was time-dependent, our present results suggest that thioacetamide might inhibit P450 enzymes in mechanism-based inactivation.

口眼渦斜 患者의 Gadolinium-DPTA enhanced MRI 所見에 대한 臨床的 考察

김재수,최우석,김용석,고형균,강성길,김창환 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

This study is designed to evaluate the clinical implications of Gd-DPTA (Gadolinium-diethyl - enetriamine pentacetic acid) enhanced MRI(Magnetic resonance imaging) in Bell's palsy and find it's usefulness in Oriental Medicine In this study, 25 outpatients with Bell's palsy were studied that MRI was performed. To evaluate degree of facial palsy, H-B(House-Brackmann) Grade was used. In Oriental Medical therapy, Acupuncture and Herbal medicine were treated. Subjective causes was divided into exposure to chill, fatigue, stress, mixed cases. Enhanced site was compared with symptoms which were disorder of eye, hearing, taste, and facial muscle palsy. Also, Relation between time which was performed MRI and enhancement was analyzed. The enhanced lesion in MRI was divided into five segments; Internal auditory canal, Labyrinthine segment, Geniculate ganglion, Tympanic segment, Mastoid segment. In Bell's palsy, 20 of 25 patients(80%) had abnormal contrast enhancement of the facial nerve. The H-B grade and interval performed MRI from onset were directly proportionate to enhancement. That is to say, Severe facial palsy short interval show high possibility of enhancement. There was no relation between subjective causes and enhanced site of facial nerve in MRI. Also Clinical symptoms didn't coincide with MRI findings.

Enhancement of Plasmacytoma Cell Growth by Ascorbic Acid is Mediated Via Glucose 6-phosphate Dehydrogenase

Woo Suk Koh,Woo Hyuck Choi,Sook Ja Lee,Chaehwa Park,Chan H Park 대한암학회 2007 Cancer Research and Treatment Vol.39 No.1

기니픽에서 HM10411 의 항원성

고우석(Woo Suk Koh),김종춘(Jong Choon Kim),차신우(Shin Woo Cha),김영민(Young Min Kim),정성엽(Sung Youb Jung),권세창(Se Chang Kwon),이관순(Gwan Sun Lee) 한국응용약물학회 2002 Biomolecules & Therapeutics(구 응용약물학회지) Vol.10 No.2

N/A HM10411 is a recombinant granulocyte-colony stimulating factor (rG-CSF) that has been developing as a drug for neutropenia. In this study, antigenic potential of HM 10411 was examined by active systemic anaphylaxis (ASA) in guinea pigs and passive cutaneous anaphylaxis (PCA) in guinea pig-guinea pig system. HM10411 was subcutaneously administered at 0, 5, and 50 ㎎/㎏ and also as a suspension with adjuvant (50 ㎎/㎏+FCA). Ovalbumin (OVA) as a suspension with adjuvant was used to induce positive control responses. In the ASA test, no symptoms except urination and evacuation that were considered as physiological phenomena were observed at 0 and 5 ㎎/㎏. Two of 5 animals at 50 ㎎/㎏ showed sneezing, dyspnea, or cyanosis. All animals in the adjuvant mixture group showed severe symptoms of anaphylatic shock and 3 of them died. In the PCA test, no antibody against HM10411 was detected in the sera from the animals sensitized with 0 or 5 ㎎/㎏. Only 1 serum sample from the animals immunized with 50 ㎎/㎏ showed positive reaction against HM10411, while all 5 sera collected from the HM10411 and FCA mixture group contained the HM10411 -specific antibodies. These results suggest HM10411 is considered to have antigenicity in guinea pig.

지능형 전력관리시스템의 구축사례

고강훈(Kang-Hoon Koh),김천식(Chun-Sik Kim),김수용(Soo-Young Kim),박정우(Jung-Woo Park),고희석(Hee-Suk Koh),이현우(Hyun-Woo Lee) 한국조명·전기설비학회 2000 한국조명·전기설비학회 학술대회논문집 Vol.2000 No.-

This paper is a study if remote control system with KEN that is not only superior-manufacture ability-hardware; that is to say, the hardware is HMI/SCADA solution and it has been developed to sub-station and power management system, which is easy, fast and suitable for users to work with, but also compatible solution with domestic environment. Futhermore, the hardware has been developed by completely domestic technology with knowledge from various actual experiences. In the long run, for uninterruptible power supply to train, building, and factory, real time tele-supervisory control to power facilities.

한국의 발톱진균증 환자의 삶의 질에 관한 연구

조백기,박종갑,김형옥,김성욱,백승철,김진우,김시용,안규중,전재복,오지원,김낙인,이규석,오칠환,김수남,김상태,손숙자,신용우,김동석,이원우,권경술,서대헌,황규왕,이종석,고재경,강원형,정기양,최응호,김기홍,박석돈,강승주,함정희,명기범,김방순,구상완,김병수,원영호,김한욱,송은섭,정병수,노병인,홍창권,박장규,한지윤,김광중,구대원,김종민,김재홍,유희준,양경미 대한의진균학회 1998 대한의진균학회지 Vol.3 No.2

Background: Onychomycosis, especially toenail onychomycosis has become one of the common fungal infection and has historically been regarded as a cosmetic rather than medical problem by many patients, even by physicians. Recently, however, there are several reports that is a refractory disease which may cause a deleterious effect on patiets quality of life (QOL). Objective: The purpose of this study was to investigate the impact of toenail onychomycosis on QOL in Korea and to assess the chandes of QOL after treatment. Methods: Total 1004 patients with toenail onychomycosis which was confirmed by clinical findings and KOH preparation were were enrolled at 47 dermatologic centers in Korea, and interviewed with standardized QOL questionnaire before and after sytemic antifungal treatment. Responses to the questionnaire were scored by 5-point scale (1∼4) and averaged, and were analyzed for 5 dimensions of emotional impact, social impact, symptorn and functional impact, patients views concerning treatment, and relationship with doctor. Results: 1. Before and after treatment, the most serious impact was emotional dimension showing 1.90 and 1.30 in average score (AS), and social (AS: 1.14 and 0.83) and symptom and functional impact (AS: 1.05 and 0.92) was also affected. 2. In female rather than male, statistically more significant impact on patients QOL was observed in all dimensions. 3. After treatment, 3 of 5 dimensions were improved significantly - emotional dimension (AS : from 1.90 to 1.30), social dimension (AS: from 1.14 to 0.83), patients view concerning treatment (AS: from 1.34 to 1.02). 4. The drgree of patients satisfaction at the therapeutic effect was very high- 62.4% (immediately after treatment) and 65.8% (9 months affer initiation of treatment) of patients answered excellent or good. Conclusion: This study confirms that toenail onychomycosis has significant impact on the overall QOL of patients. Also the effect of antifungal therapy on patients QOL were watisfactory. Therefore, both doctor and patient should pay more attention to the treatment of onychomycosis. [Kor J Med Mycol 3(1): 115∼124]

Elevated cAMP level attenuates 2-deoxy-D-ribose-induced oxidative damage in pancreatic β-cells

Koh, Gwanpyo,Suh, Kwang Sik,Chon, Suk,Oh, Seungjoon,Woo, Jeong-taek,Kim, Sung-Woon,Kim, Jin-Woo,Kim, Young-Seol KYUNG HEE UNIVERSITY MEDICAL CENTER 2006 고황의학상 수상논문집 Vol.21-22 No.-

Glucose toxicity to pancreatic β-cells is defined as irreversible β-cell damage, including apoptosis, caused by chronic exposure to high glucose levels in type 2 diabetes. Oxidative stress is an important mechanism for glucose toxicity to pancreatic β-cells. Reducing sugars produce reactive oxygen species through autoxidation and protein glycosylation. 2-Deoxy-D-ribose (dRib) is a reducing sugar with high reactivity. We investigated whether cAMP-stimulating agents could protect β-cells from dRib-induced oxidative damage. HIT-T15 cells were cultured with various concentrations of dRib for 24h. We measured cell survival, intracellular cAMP and H_(2)O_(2) levels, and apoptosis. dRib decreased cell survival in a dose-and time-dependent manner and markedly increased intracellular H_(2)O_(2) levels and apoptosis. N-Acetyl-L-cysteine decreased dRib-induced rises in intracellular H_(2)O_(2) and apoptosis to control levels. Forskolin, IBMX, and dbcAMP markedly elevated intracellular cAMP levels and significantly attenuated dRib-induced cytotoxicity and apoptosis, but had no influence on the dRib-induced rise in intracellular H_(2)O_(2) levels, These results demonstrate that dRib produced oxidative stress and apoptosis in pancreatic β-cells and that elevated intracellular cAMP levels reduced dRib-induced damage, independent of reactive oxygen species metabolism.

A-431 세포주의 방사선 및 항암제의 감수성에 관한 실험적 연구

고광준,최은숙,홍성우 大韓口腔顎顔面 放射線學會 1999 Imaging Science in Dentistry Vol.29 No.1

Objectives: The purpose of this study was to aid in the prediction of tumor cell tolerance to radiotherapy and/or chemotherapy. Material and Methods: Human epidermoid carcinoma A-431 cell lines were irradiated by 2, 4, 6, 8, 10Gy at a dose rate of 210cGy/min using Co Irradiator ALDORADO 8 and then were exposed to bleomycin or cisplatin at concentration of 2㎍/ml for 1 hour. The viable cells were determined for each radiation dose and/or each drug at the 4th day and cell surviving curves were obtained using semiautomated MTT assay. Results: The surviving fraction after irradiation of 2Gy was 0.99, and there was not significant difference of surviving fraction in comparison with the control group on A-431 cell line(P>0.05). But there were significant differences of surviving fractions at doses of 4, 6, 8, 10Gy in comparison with the control group(P<0.05). The cytotoxicity of bleomycin or cisplatin was significantly different in comparison with the control group on A-431 cell line (P<0.05). And the cytotoxicity of cisplatin was greater than that of bleomycin on A 431 cell line (P<0.05). There were significant differences of surviving fractions after irradiation of 2, 4, 6, 8, 10Gy with bleomycin or cisplatin in comparison with each group of irradiation only on A-431 cell line(P0.05). There were significant differences of surviving fractions between the groups of irradiation with bleomycin and cisplatin at doses of 2, 4Gy(P<0.05), but there were not significant differences of surviving fractions at doses of 6, 8, 10Gy on A-431 cell line(P>0.05).(J Korean Oral Maxillofac Radiol 1999:29:325-337)

시험관내 KB세포주의 방사선 및 항암제감수성에 관한 연구

고광준,최은숙,홍성우 大韓口腔顎顔面 放射線學會 1998 Imaging Science in Dentistry Vol.28 No.1

The purpose of this study was to aid in the prediction of tumor cell tolerance to radiotherapy and/or chemotherapy. For this study, cell surviving curves were obtained for human squamous cell carcinoma KB cell line after radiation exposure and/or administration of antitumor drugs. 2, 4, 6, 8, 10Gy were irradiated at a dose rate of 210cGy/min using ?? Irradiator ALDORADO 8. After irradiation. KB cell lines(3×104cells/ml) were exposed to 2㎍/ml of bleomycin or cisplatin for 1 hour. The viable cells were determined by MTT assay for each radiation dose and/or each drug at the 4th day. And they were compared to control values. The obtained results were as follows: 1. The slope of the surviving curve after irradiation of 2, 4, 6, 8, 10Gy on KB cell line was relatively steep. 2. There was no significant difference between the cytotoxicity of bleomycin compared to control group. But, there was significant difference between the cytotoxicity of cisplatin compared to control group. And the cytotoxicity of cisplatin was greater than that of bleomycin on KB cell line. 3. There were significant differences of surviving fractions after irradiation of 2Gy and 10Gy with 2㎍/ml of bleomycin compared with the groups of irradiation only on KB cell line. 4. There were significant differences of surviving fractions after irradiation of 2, 4, 6, 8, 10Gy with 2㎍/ml of cisplatin compared with the groups of irradiation only on KB cell line. 5. There was significant difference of surviving fraction between groups after irradiation of 10Gy with 2㎍/ml of bleomycin and cisplatin.

Sensitization of ionizing radiation-induced apoptosis by ursolic acid

Koh, Su Jin,Tak, Jean Kyoung,Kim, Seon Tae,Nam, Woo Suk,Kim, Sung Youl,Park, Kwon Moo,Park, Jeen-Woo Informa Healthcare 2012 Free radical research Vol.46 No.3

<P>Radiation therapy has been widely used for treating human cancers. However, cancer cells develop radioresistant phenotypes that decrease the efficacy of radiotherapy. Ionizing radiation (IR) induces the production of reactive oxygen species, which play an important role in apoptotic cell death. Therefore, radiation therapy combined with a sensitizer, which modulates cellular redox status, has the potential to enhance therapeutic efficacy in a variety of human cancers. Here, we investigated the radiosensitizing effects of ursolic acid (UA), a pentacyclic triterpenoid found in rosemary and holy basil. IR-induced apoptosis in cancer cell lines such as DU145, CT26 and B16F10 was significantly enhanced by UA, as reflected by DNA fragmentation, cellular redox status, mitochondrial dysfunction and modulation of apoptotic marker proteins. Additionally, UA combined with IR was also effective for inhibiting tumorigenesis in B16F10 melanoma cells implanted into mice. Taken together, these results suggest that applying UA together with IR may be an effective combination modality for treating cancer.</P>