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고우석(Woo Suk Koh),김종춘(Jong Choon Kim),차신우(Shin Woo Cha),김영민(Young Min Kim),정성엽(Sung Youb Jung),권세창(Se Chang Kwon),이관순(Gwan Sun Lee) 한국응용약물학회 2002 Biomolecules & Therapeutics(구 응용약물학회지) Vol.10 No.2
N/A HM10411 is a recombinant granulocyte-colony stimulating factor (rG-CSF) that has been developing as a drug for neutropenia. In this study, antigenic potential of HM 10411 was examined by active systemic anaphylaxis (ASA) in guinea pigs and passive cutaneous anaphylaxis (PCA) in guinea pig-guinea pig system. HM10411 was subcutaneously administered at 0, 5, and 50 ㎎/㎏ and also as a suspension with adjuvant (50 ㎎/㎏+FCA). Ovalbumin (OVA) as a suspension with adjuvant was used to induce positive control responses. In the ASA test, no symptoms except urination and evacuation that were considered as physiological phenomena were observed at 0 and 5 ㎎/㎏. Two of 5 animals at 50 ㎎/㎏ showed sneezing, dyspnea, or cyanosis. All animals in the adjuvant mixture group showed severe symptoms of anaphylatic shock and 3 of them died. In the PCA test, no antibody against HM10411 was detected in the sera from the animals sensitized with 0 or 5 ㎎/㎏. Only 1 serum sample from the animals immunized with 50 ㎎/㎏ showed positive reaction against HM10411, while all 5 sera collected from the HM10411 and FCA mixture group contained the HM10411 -specific antibodies. These results suggest HM10411 is considered to have antigenicity in guinea pig.
이의주,정용재,곽창규,황민우,유정희,고우석,김경수,고병희,Lee, Eui-Ju,Jung, Yong-Jae,Kwak, Chang-Kyu,Hwang, Min-Woo,Yoo, Jung-Hee,Ko, Wo-Suk,Kim, Kyung-Soo,Koh, Byung-Hee 사상체질의학회 2007 사상체질의학회지 Vol.19 No.2
1. Objectives In oriental medicine, there is no method to diagnosis Sasang constitution of school aged children. The objective diagnostic method is necessary to improve the health condition of children. The method must be able to reduce time and to correct subjective error. So the purposes of this study are developing the questionnaire to diagnosis Sasang constitution of school-aged children. 2. Methods The questionnaire consists of selected substance of ${\ulcorner}$Dongyisusebowon${\lrcorner}$, characteristic questionnaire of children and questionnaire for the Sasangin Diagnosis Questionnaire(for adult). Experts, element school children and a scholar on Korean literature revise the questionnaire. 3. Results and Conclusion Experts examed pre-questionnaires and selected the final questionnaries by CVI 0.8 at propriety of contents. Sasangin Diagnosis Questionnaire (SDQ) for Child consists of 84 questions (24 questions for child, 48 questions and 12 questions for doctor).
연구논문 : 생명과학 ; Thioacetamide 유발 면역독성에 있어서 FMO에 의한 대사과정의 역할에 관한 연구
이정운 ( Jeong Woon Lee ),신기덕 ( Ki Duk Shin ),이미가엘 ( Michael Lee ),김은주 ( Eun Joo Kim ),한상섭 ( Sang Seop Han ),한미영 ( Mi Young Han ),하현정 ( Hyun Jung Ha ),정태천 ( Tae Cheon Jeong ),고우석 ( Woo Suk Koh ) 영남대학교 약품개발연구소 2003 영남대학교 약품개발연구소 연구업적집 Vol.13 No.-
Thioacetamide에 의한 BALB/c 마우스 간의 시간별 약물대사효소 억제 양상 : A Time-Course Study
이정운,고우석,김갑호,배연경,하현정,한상섭,천영진,정태천 영남대학교 약품개발연구소 2001 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-
Thioacetamide is a potent hepatotoxicant which requires metabolic activation by cytochrome P450s (P450s) for toxicity. In the present study, the elevation kinetic of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities by thioacetamide treatment was investigated in male BALB/c mice. Inaddition, the inhibitory effects of thioacetamide on liver microsomal P450 enzymes were further investigated. Thioacetamide at 100 mg/kg/ was treated intraperitoneally for 6, 12, 24, 36, 48 and 72 hr. The blood was collected at the designated time for assaying the serum enzyme activities. To determine the P450 isozyme-specific activities. ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and benzyloxyresorufin O-debenzylase (BROD) activities were determined for P450 1A1, 1A2 and 2B1, respectively, in liver microsomal fractions. The activities of ALT and AST were started to be elevated 6 hr after thioacetamide treatment andreached the maximun at 36 hr after the treatment. The elevated activities were dramatically recovered at 72 hr. The microscopic exmination of the liver specimen also showed a similar profile of hepatotoxicity. All P450-associated enzyme activities were time-dependently inhibited by the treatiment with thioacetamide. The maximum inhibition of P450 enzymes was observed 36 hr after the treatment. Because the inhibition of P450 enzymes by thioacetamide was time-dependent, our present results suggest that thioacetamide might inhibit P450 enzymes in mechanism-based inactivation.
Human Multiple Myeloma 세포주 U266에서 IL-6에 의한 Grb2 결합 단백질들의 인산화
윤선영,고우석,이은경,최인표,한미영 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.4
Interleukin-6 (IL-6) is a pleiotropic cytokine generating multifunctional signals to several cell lines such as B cells, T cells, hepatocytes, myeloma, and leukemic cell lines. To understand the diverse signals generated by IL-6, it is essential to clarify the molecular and cellular events following IL-6 stimulation. IL-6 binding to its receptor triggers the association of the cytoplasmic domain of the receptor (gp80) with a signal transducing unit of IL-6R, gpl3O. Since neither gp80 nor gp130 have any protein tyrosine kinase domain, unknown protein kinases except Janus kinase might be involved in the early steps of IL-6 signal transduction. Like many other cytokines and growth factors, IL-6 activates p2l'az. However the precise biochemical mechanism leading to the p21' activation is still unclear. It was recently elucidated that she (src homology and collagen) known to activate p21' affected on IL-6 signal pathway. Therefore we investigated the effects of IL-6 on Grb2 (growth factor receptor bound protein 2), which is an element of the Ras pathway in multiple systems. In multiple myeloma cell line U266, IL-6 induced the tyrosine phosphorylation of several Grb2-associated proteins 80, 66, 52 and 44kD in a time-dependent manner. We also observed several protein kinases or substrates, including 44 and 25kD protein, by in vitro kinase assay. These findings suggest that IL-6 might activate the Ras signalling pathway via tyrosine phosphorylation of several Grb2-associated proteins. Futher studies will be need to elucidate which of the IL-6 receptor-associated cytoplasmic tyrosine kinases or adaptor proteins mediate IL-6 signalling pathway.
IL-6 의존형 B 하이브리도마 세포의 신호전달에서 Grb2 단백질의 역할
윤선영,고우석,최인표,권병목,정태화,한미영 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.2
IL-6 is a pleiotropic cytokine generating multifunctional signals to several cell lines such as B cells, T cells, hepatocytes, myeloma, and leukemic cell lines. To understand the diverse signals generated by IL-6, it is essential to clarify the molecular and cellular events following IL-6 stimulation. Initially IL-6 binds to cells with its binding subunit of IL-6R(gp80). The binding triggers the association of gp80 with a signal transducer, gp130. Since neither gp80 nor gp130 have any protein tyrosine kinase domain, unknown protein kinases except Jak kinase might be involved in the early steps of IL-6 signal transduction. Growth factor receptor-bound protein 2 (Grb2) is a 25 kD adaptor protein composed of a Src homology 2 (SH2) domain and two flanking Src homology 3 (SH3) domains. In order to identify phosphoproteins associated with Grb2 adaptor protein, we have constructed a GST-Grb2 fusion protein and isolated its associated tyrosine phosphoproteins, including 72, 70, 46 and 28 KD that were phospholyated with IL-6 stimulation in B 9-79 hybridoma cells. We also detected several protein kinases, including 50 KD and 44 KD protein by in vitro kinase assay. These results suggest that several phosphoproteins and kinases are involved in IL-6 signal transduction.
Role of corticosterone in ethyl carbamate-induced immunosuppression in female BALB/c mice
Cha, Shin Woo,Lee, Hu Jang,Cho, Myung Haing,Lee, Mun Han,Koh, Woo Suk,Han, Sang-Seop,Kim, Jung-Ae,Lee, Eung-Seok,Nam, Doo-Hyun,Jeong, Tae Cheon 영남대학교 약품개발연구소 2001 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-
We have recently demonstrated that the antibody response to the T-cell-dependent antigen, sheep red blood cells(SRBCs), was suppressed by ethyl carbamate in female BALB/c mice. At the same doses, ethyl carbamate decreased in the numbers of splenic macrophages. B cells, total T cells, CD4^(+) T cells and CD8^(+) T cells. In addition, the serum level of corticosterone was increased dose-dependently. To investigate the possible role of corticosterone in ethylcarbamate-induced immunosuppression, the antibody response to SRBCs and the subpopulation changes of splenocytes and thymocytes were determined in naive, sham-operated and adrenalectomized (ADX) female BALB/c mice. When the mice were treated intraperitoneally with 400 mg/kg ethyl carbamate, the antibody response was significantly suppressed by ethyl carbamated in naive and sham-operated mice in accompanying the decrease in spleen and thymus weights and/or the increase in the level of serum corticosterone. Meanwhile, the antibody response was not suppressed by ethyl carbamate in the ADX mice. The splenic numbers of total cells, macrophages B and T cells, and CD4^(+) cells were decreased by ethyl carbamate in naive and sham-operated mice. Meanwhile, each cell number was comparable with control in the ADX mice. The flow cytometric analyses on thymocytes did not show obvious differences as seen in the spleen. Finally, when the ADX mice were treated intraperitoneally with 25 mg/kg corticosterone, the antibody response was significantly suppressed. Taken together, our present results suggested that corticosterone might be, at least partially, responsible for ethy; carbamate-induced immunosuppression in female BALB/c mice. ⓒ 2001 Elsevier Science Ireland Ltd. All rights reserved.
IL-1 수용체를 통한 T세포 활성화에 Genistein 과 Staurosporin이 미치는 영향
한미영,윤도영,오은숙,고우석,윤선영,정태화 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.4
Stimulation of T-cells through TCR/CD3 complex alone is known to be not enough but also require a costimulatory signal, such as IL-1 stimulation. to induce IL-2 production. In this report, we studied the differential effects of staurosporin (a PKC inhibitor) and genistein (a PTK inhibitor) on the IL-2 production by EL-4 and A2.5 cells, to determine which pathway is responsible for IL-1 receptor signal transduction. While staurosporin suppressed the IL-2 production induced in EL-4 cells upon PMA stimulation but not upon PHA plus IL-1, genistein inhibited the IL-2 production induced by PHA plus IL-1 but not by PMA. Genistein also completey abrogated the IL-2 production by A2.5 cell when stimulated with PHA and IL-1. These results suggest that the costimulatory signal by IL-1, which leads to IL-2 production and proliferation, is closely linked to the PTK activity in T cells.