육군 범법자들에 대한 정신의학적 특성 분석

이규환,박흥수,이정태 大韓神經精神醫學會 1989 신경정신의학 Vol.28 No.2

The authors compared two hundred forty-eight imprisoned soldiers with two hundred seventy-nine normal controls using MMPl and Ego-Strength Scale in R.0.K. Army. The results were as follows ; 1) Mean age of imprisoned soldiers was 23.4, while that of controls was 23.9. 2) Educational level of imprisoned soldiers was lower than that of normal controls. 3) Pre-enlistment occupations in imprisoned soldiers chiefly were employees and factory workers (39.9%), while students consisted of 54.4% in normal controls. 4) More than half(61.4%) of the criminal acts had occured during the first year of the military service 5) Of the various criminal acts, absence without leave was the most frequent criminal activities(50.6%). 6) The result of MMPI was significantly different between two groups except L subscale. More specifically, F, Ma, Sc, Pd, Pa subscales which indicate a tendency of behavioral acting out were high in imprisoned soldiers, but all subscales showed below scores of 65. 7) Scores of Ego-Strength Scale in imprisoned soldiers were lower than that of the controls.

진행성 붉은 포도주형 혈관종의 복합 레이저 치료

이태권,장덕규 한국의학사 1993 診斷과治療 Vol.13 No.8

진부지역에서 생산된 당귀를 이용한 추출물 제조 및 이화학적 특성

이원종,윤정로,김은경,안규태 강릉대학교 동해안지역연구소 2000 東海岸硏究 Vol.11 No.1

추출조건 (온도, 추출시간, 용매의 농도)에 따라 당귀의 특유성분인 decursin의 함량을 측정하였으며, 산지별로 당귀를 수집하여 추출물을 제조한 후 이화학적 성질을 조사하였다. 물, ethanol, methanol, chloroform, benzene, ether등으로 추출한 결과, 당귀의 지표물질인 decursin은 물로는 잘 추출되지 않았고 고농도의 ethanol로 추출하는 것이 가장 효과적이었다. 당귀의 ethanol 추출물 제조시 ethanol의 농도는 95%, 추출온도는 60℃, 추출시간은 4시간이 가장 적합하였다. 진부지역에서 생산된 일당귀 및 일본산 당귀의 decursin 함량은 0.2-0.5%이었고, 중국산 당귀는 1.1%를 함유하였으며 진부산 토당귀는 6.2%를 함유하였다. 진부산 토당귀와 일당귀, 중국산, 일본산 당귀를 열수 추출물, ethanol 추출물을 제조한 결과, 열수 추출물은 ethanol 추출물보다 단백질과 회분을 많이 함유하였으며, ethanol 추출물은 지질을 많이 함유하였다. Chemical components and decursin contents of Angelica gigas Nakai (Korean), Angelica acutiloba Kitagawa (Korean and Japanese), and Angelica sinensis Diels (Chinese) were analyzed. Angelica gigas Nakai (Korean) contained the highest amount of decursin with 6.2%, while Angelica acutiloba Kitagawa (Korean and Japanese) contained only 0.2-0.5% decursin and Angelica sinensis Diels (Chinese) contained 1.1% decursin. Angelica gigas Nakai (Korean) was extracted under various conditions and evaluated for decursin content. The decursin content of extracts was the highest when extracted with 95% ethanol at 60℃ for 4 hours. Angelica gigas Nakai was extracted with water and 95% ethanol and physicochemical properties of extract were analyzed, The extraction yield. protein content, and ash content were high when using the water as an extraction solvent; while, crude lipid was high when using the 95% ethanol as the extraction solvent.

Cadmium Chloride가 흰쥐 신장에 미치는 영향에 관한 조직화학적 연구

이규식,백두진,김원규,정호삼,백태경,김영학 漢陽大學校 環境科學硏究所 1993 環境科學論文集 Vol.14 No.-

중공업의 발달에 따라 공장등지에서 중금속인 cadmium을 이용한 제품의 생산이 증가함에 따라 자연계내 토양, 물, 식물 등에 함유된 cadmium 뿐 아니라 공업제품과 공업 쓰레기에 함유된 cadmium은 쉽게 인체에 폭로되어 인체에 유해한 결과를 초래하게 된다고 알려져있다. 이에 저자는 cadmium이 생체내 신장에 특히 오랜기간 축적된다는 보고와 또 반감기가 긴 중금속이란 점에 착안하여 신장의 피질과 수질내 조직학적 변화를 관찰하고자 하였다. 실험동물은 사육실에서 순종 분리고 4년간 사육한 Wistar계 흰쥐를 사용하였다. 흰쥐에 체중 kg당 0.6mg의 cadmium chloride를 식염수 0.2ml에 용해하여 복강내로 10주간 매주 1회씩 투여한 후 희생기켜 신장조직을 paraffin 표본으로 제작하고 Mallory-Azan 염색을 시행하여 광학현미경으로 관찰한 후 다음과 같은 결과를 얻었다. 1. Cadmium chloride 10주간 투여후 1일 경과군에서 신장의 피질과 수질에 강한 Azan 염색반응을 보였으며 신사구체의 괴사와 수질 일부에서 간질성 섬유증식 및 울혈이 관찰되었다. 2. Cadmium chloride 10주간 투여후 1주 경과군에서는 신장의 피질과 수질에 중등도의 Azan 염색반응이 관찰되었고 신사구체의 국소적 괴사가 관찰되었다. 3. Cadmium chloride 10주간 투여후 2주 경과군에서는 신장의 피질에 중등도의 Azan 염색반응이 관찰되었으며, 4주 경과군에서는 피질과 수질에 약한 Azan 염색반응만 관찰되었다. 이상과 같은 결과로 미루어 cadmium chloride의 흰쥐에 장기간 투여는 미량이라도 신장의 신사구체의 괴사, 섬유화 등이 유발되나 투여후 4주 경과시는 손상이 회복되는 것으로 사료된다. The peritoneal injections of cadmium chloride(0.6mg/kg) per 1 week for 10 weeks were made. The cadmium chloride treated rats were sacrificed on 24 hours, 1 week, 2 weeks and 4 weeks after 10 weeks administration. And each of renal tissues from cadmium treated rat kidney was stained with Mallory Azan. 1. The severe glomerular necrosis, fibrosis and congestions in 1 day group were seen in the cadmium chloride treated rats medulla. 2. The moderately positive reaction for azan stain was seen in cortex and medulla of 1 week group in the cadmium chloride treated rats medulla. 3. The weakly positive reaction for azan stain was revealed in cortex and medulla of the rat kidenys on 2 weeks and 4 weeks after cadmium chloride administration for 10 weeks. It is concluded that the renal damage of rat resulted with administration of cadmium chloride may be recovered 4 weeks after injection.

Vincristine Sulfate가 백서 척수내 신경원의 Acetylcholinesterase 활성에 미치는 영향

이규식,이군자,정호삼,김승현,백태경 漢陽大學校 環境科學硏究所 1992 環境科學論文集 Vol.13 No.-

Vincristine은 협죽도과에 속하는 열대식물인 Madagascum periwinkle에서 추출한 천연 alkaloid계 항암제로서 암세포의 미소관 형성을 억제하고 미소관을 파괴하며 또한 핵산 및 단백질합성을 방해하여 암세포의 증식을 억제하므로 현재 백혈병 및 임파종등 혈액종양과 여러 충실성종양의 치료에 유용하게 사용된다. 그러나 다른 항암제와 마찬가지로 암세포에만 특이하게 작용하지 않고 정상세포에도 손상을 야기하므로 vincristinc을 투여받은 암환자에서 혈액독작용, 소화기장애, 피부장애 및 신경독작용등 여러 부작용이 유발되고 특히 신경독 작용은 vincristine을 사용을 제한하는 가장 심각한 장애로 알려져있다. 저자는 백서에 vincristine을 투여하면 vincristine이 실험동물의 척수내 신경원에 손상을 야기하여 척수내 신경원의 acetylcholinesterase활성에 변화가 초래될 것으로 사료되어 본 실험을 시도하였다. 실험동물로는 체중 200gm내외의 Wistar계의 웅성 백서 35마리를 사용하여 백서 체중 kg당 0.5mg의 vincristine sulfate(Abic Ltd.) 0.2cc를 1일 1회씩 5일간 복강내로 주사하였고 정상대조군 백서에는 동량의 생리적 식염수를 같은 방법으로 투여하였다. 실험동물은 최종 주사후 24시간 및 72시간 경과 후에 후두부 강타로 도살하고 10% neutral buffered formaline으로 관류시킨 후 육안으로 해부하여 척수를 절취하고 10% neutral buffered formaline에 4℃에서 18시간 고정후 일부 조직은 paraffin에 포매하여 Nissl체를 관찰하기 위한 cresyl violet염색을 시행하고, 일부조직은 -20℃로 조절된 cryostat로 두께 10㎛의 동결절편을 제작하여 Gredtzoff(1959)의 방법에 따라 acetyl-cholinesterase활성을 관찰하기 위한 조직화학적 처리를 한 후 광학현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. Vincristine투여군 백서의 척수내 체성운동신경원 및 내장성운동신경원에 chromatolysis가 유발되었으며, 소신경원의 수와 cresyl violet 에 대한 염색성이 감소되었다. 2. Vincristine투여군 백서 척수경부의 acetylcholinesterase활성은 1일 경과군의 복측각내 체성 운동신경원에서는 강한 양성반응을 나타내었고 배측각에서는 약한 양성반응을 나타내었으며 중간부에서는 미약한 양성반응을 나타내었으나 3일 경과군의 복측각내 체성운동신경원과 배측각내 교양질부에서는 중등도 양성반응을 나타내었으며 중간부에서는 미약한 양성반응을 나타내었다. 3. Vincristine투여군 백서 척수흉부의 acetylcholinesterase활성은 1일 경과군의 복측각내 체성운동신경원과 외측각내 내장성운동신경원에서는 약한 혹은 중등도 양성반응을 나타내었고 배측각에서는 약한 양성반응을 나타내었으며 척수수질의 중간부에서는 미약한 양성반응을 나타내었으나, 3일 경과군의 복측각내 체성운동신경원에서는 중등도 양성반응을 나타내었고 외측각내 내장성 운동신경원에서는 강한 양성반응을 나타내었으며 배측각내 교양질부에서는 중등도 양성반응을 나타내었으나 배측각의 나머지 부분에서는 약한 양성반응을 나타내었고 척수수질의 중간부에서는 미약한 혹은 약한 양성반응을 나타냈었다. 4. Vincristine 투여군 백서 척수요부의 acetylcholinesterase활성은 1일 경과군의 복측각내 체성운동신경원에서는 중등도 혹은 강한 양성반응을 나타내었고 배측각의 교양질부와 Lamina Ⅳ에서는 중등도 양성반응을 나타내었으며 배측각의 나머지 부분은 약한 양성반응을 나타내었고 척수수질의 중간부에서는 미약한 양성반응을 나타내었으나, 3일경과군의 복측각내 체성운동 신경원에서는 약한 혹은 중등도 양성반응을 나타내었고 배측각내 교양빌부와 Lamina Ⅳ에서는 약한 양성반응을 나타내었으며 척수수질의 중간부에서는 미약한 혹은 약한 양성반응을 나타내었다. 이상의 소견을 종합한 바, vincristine은 백서 척수내 신경원에 세포독으로 작용하여 신경원 손상을 초래하고 신경원의 acetylchlinesterase활성을 억제 하나, 이러한 손상은 시간이 경과함에 따라 일부 회복되는 것으로 사료된다. Vincristine, one vinca alkaloids, is widely used for chemotherapy of a variety of malignancy. The mechanism of anticancerous activity of this agent is based on inhibition of mitosis by interference with development of microtuble, of syntheses of nucleic acid and protein, and of destruction of cytoplasic membrane and microtubule. However, these inhibitory effects on cancer cell proliferation are non-specific, so clinical use of vincristine is frequently limited due to its toxic effects on the hematopoietic and nervous system especially. The author had undertaken this study to pursue the effect of vincristine on the nuerons in rat spinal cord histolgically and histochemically. Totals of 35 healthy male Wistar stain albino rats, weighing about 200 gm, were used as experimental animals. The experimental animals were divided into normal control and vincristine treated groups, and then vincristine treated rats were subdivided into 24 hours and 72hours groups by time passage. All vincristine treated rats were abministrated intraperitoreally with 0.5mg of vincristine per kg of rat body weight diluted in 0.2cc of normal saline for 5 days and rats of vincristine treated group were sacrificed by occipital blow at 24 hours and 72 hours after administration and then perfused by 10% neutral buffered formaline. The spedimen, spinal cord was collected and them fixed with 10% neutral buffered formaline during 18 hours at 4℃. For observing the histological change, cresyl violet stain was performed by routine pareffin method, and for histochemical study of acetylcholinesterase, frozen sedtions of 10 ㎛ in thickness were treated with Gerebtwoff method(1959). The results obtained were as follows : 1. In vincristine treated rat, a part of the somatic and visceral motor neurons were brought on chromatolysis. And the numer and the stain activities of small neurons were decreased in cresyl violet stain. 2. The activities of acetylcholinesterase of cervical segment in vincristine treated rat spinal cord were strongly positive at somatic motor meuron, weakly positive at dorsal horn and tracely positive at intermediate zone in 1 day passed group after the final administration, and moderately positive at somatic motor neuron and substantia gelatinosa, weakly positive at remainder of dorsal horn and tracely positive at intermediate zone in 3 days passed group. 3. The activities of acetylcholinesterase of thoracic segment in vincristine treated rat spinal cord weakly or moderately positive at somatic and visceral motor neurons, weakly positive at dorsal horn and tracely positive at intermdeiate zone in 1 day passed group after the final administration, and moderately positive at somatic motor mourons, strongly positive at visceral motor meurons, moderately positive in substantia gelatinosa, weakly positive at remainder of dorsal horn and tracely positive at intermediate zone in 3 days passed group. 4. The activities of acetylcholinesterase of lumbar segment in vincristine treated rat spinal cord were moderately or strong positive at somatic motor meurons, weakly positive at substantia gelatinosa and lamina Ⅳ, weakly positive at remainder of dorsal horn and tracely positive at intermediate zone in 1 day pssed group after the final administration, weakly or moderately positive at somatic motor neurons, weakly positive at sugstantia gelationsa and lamia Ⅳ and tracely or weakly positive at remainder of spinal medulla in 3 days passed group. It is consequently suggested that vincristine would induce the degeneration of neurons and inhibit acetylcholinesterase activity resulting from its neurotoxic effects on neurons in spinal cord of albino rats, but this damage would partially recover by time passage.

흰쥐 정소 간질세포의 발생에 관한 형태학적 연구

이법이,백태경,정호삼,이규식,박준영 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

It is well known that interstitial cell of Leydig is developed from the mesenchymal cell in the intestitial tissue of the testis during fetal period, but it gradually regressed until puberty when mature Leydig cell is derived from the mesenchyme of interstitial tissue of fetal Leydig cell. The function of the Leydig cell is synthesis and secretion of testosterone which influences on differentiation of male geritalia at fetal period, and revealation of male secondary sexual characteristies and differentiation of sperm at puberty. To investigate the differentiation of interstitial cell in interstitium and ultrastructural changes of interstitial cell in rat testis and ultrastructural changes of interstitial cell in rat testis and relation to testosterone secretion, the author has studied this experiment. Fetal rats on the 16th, 18th and 20th gestational days and rats on the 1st, 3rd, 7th day, 2nd, 3rd and 8th week after birth were used as experimental animals. Some of obtained testis were stained with hematoxylin and eosin for light microscopic study, and other specimens were double stained with uranyl acetate and lead citrate for electron microscopic study. The results were as follows: 1. On the 16th day of pregnancy, a few of round eosinophilic Leydig cells are firstly observed in the interstitium of testis in fetal rat. On the 18th and 20th day of pregnance, 1st and 3rd day of neonatal life, a number of Leydig cells forming cellular cord were observed. On the 1st and 2nd week after birth, number and size of Leydig cell are markedly reduced but on teh 3rd week after birth Leydig cells are reappeared. On the 8th week after birth distribution of Leydig cells is similar to that in adult rat testis. 2. On the 18th and 20th day pregnancy, and 1st and 3rd day of neonatal life, circular or oval shaped mitochondria matrix were observed in the Leydig cell. On the 1st and 2nd week after birth, giant mitochondria and mitochondria with disorganized mitochondrial crista were revealed. On the 3rd and 8th week after birth, appearances of mitochondria were similar to that in Leydig cell of the rat testis on the 20th day of pregnancy. 3. Numerous and round cisternae of smooth endoplasmic reticulum were observed in Leydig cell of rat testis on the 18th and 20th day of pregnancy. 1st and 3rd day of neonatal life, and 1st week after birth . Horseshoe-shaped deformity and reduction in number of cisternae of smooth endopalmic reticulum were revealed in Leydig cells on the 2nd week after birth. Numerous and round cisternae of smooth endoplasmic reticulum were seen on the 3rd and 8th week after birth. 4. A few lipid droplets were revealed in Leydig cell of rat testis on the 18th day of pregnancy, but many lipid droplets were observed in Leydig cells of rat testis on the 20th day of gestation, 1st and 3rd day of neonatal life, and 1st week after birth. Numbers of lipid droplets were gradually decreased after 2nd week of postnatal life. 5. Many autophagic vacuoles were observed in Leydig cells would be firstly differentiated from the mesenchymeal cells in interstitium of fetal rat testis on the 16th day of pregnancy, and proliferated from the 18th day of pregnancy to the 1st day of neonatal life, and then gradually regressed before the puberty, but at the begining of puberty adult type of Leydig cell be proliferated.

臺灣海峽兩岸關係와 인권문제

이규태 江陵大學校統一問題硏究所 1999 統一問題硏究 Vol.15 No.-

신우황청심원액의 심혈관계에 관한 약효연구

조태순,이선미,김낙두,허인회,안형수,권광일,박석기,심상호,신대희,박대규 충남대학교 약학대학 의약품개발연구소 1997 藥學論文集 Vol.13 No.-

In order to investigate the pharmacological properties of New Woohwangchungsimwon Liquid (NCL), efects of Woohwangchungsimwon Liquid (CL) and NCL were compared. In isolated rat aorta, NCL and CL showed the relaxation of blood vessels in maximum contractile response to phenylephrine (10^6M) without regard to intact endothelium or denuded rings of the rat aorta. Furthemore, the presences of the inhibitor of NO synthase and guanylate cyclase did not affect the relaxation of NCL and CL. NCL and CL inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dosedependent manner. In conscious spontaneously hypertensive rats (SHRs), NCL and CL significantly decreased heart rate. NCL and CL, at high doses, had a negative inotropic effect that was a decrease of LVDP and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In excised guinea-pig papillary muscle, NCL and CL had no efects on parameters of action potential at low doses, whereas inhibited the cardiac contractility at high doses. These results suggested that NCL and CL have weak cardiovascular effects with relaxation of blood vessels and decrease of heart rate, and that this effect is no significant differences between two preparations.

접촉산화수로공법에 의한 수질개선연구

김태철,이규승,오범룡,민진우 忠南大學校 環境問題硏究所 1998 環境硏究 Vol.16 No.-

The effect of non-point source pollution in rural area has much influence on the stream water quality. It is difficult to improve the stream water quality, because of complex pollutant loads technically and because of treatment-cost of the deteriorated water economically. The best way to reduce the non-point source pollution is to check the water quality in the inlet of irrigation channel and control the water quality in the outlet of drainage channel. Stream water quality in the rural area is closely related with the fertilizers, pesticides, and livestock wastewater. Basically the rate of treatment and retention time were estimated for BOD, COD, SS, NH₄-N, T-N, NO₃-N, T-P, PO₄-P using materials such as gravel, crushed stone, useless tire, geotextiles, and concrete block, respectively and also using the combined materials. The rate of treatment for BOD, COD, SS is high, but that for NH₄-N, T-N, NO₃-N, T-P, PO₄-P is low. Optimal retention time in the natural contact channel was 90 minutes.

신원방우황청심원의 심혈관계에 관한 약효

조태순,이선미,김낙두,허인회,안형수,권광일,박석기,심상호,신대희,박대규 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

Abstract-In order to investigate the phamacological properties of New Wonbang Woohwangchungsimwon Pill (NSCH), effects of Wonbang Woohwangchungsimwon Pill (SCH) and NSCH were compared using various experimental models. In rat aorta, NSCH and SCH made the relaxation of blood vessels in maximum contractile response to phenylephrine (10^-6 M) regardless to endothelium containing or denuded rings of the rat aorta. Furthermore, the presence of the inhibitors of NO synthase and guanylate cyclase did not aftect significantly the relaxing effects of NSCH and SCH, NSCH and SCH inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dose-dependent manner. In conscious spontaneously hypertensive rats (SHRs). NSCH and SCH decreased significantly heart rate. These, at high doses, had a negative inotropic effect that was a decrease of left ventricular developed pressure and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In guinea-pig papillary muscle, these had no effects on parameters of action potential such as action potential amplitude (APA). V_max and resting membrane potential (RMP) at low doses, whereas inhibited the cardiac contractility at high doses. Furthermore, these had a significant inhibitory effects on palpitation of the heart in normotensive rats and SHRs. These results suggest that NSCH and SCH have weak cardiovascular effects, and that there is no significant differences between cardiovascular effects of two preparations.