DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

도시공원의 이용후 평가에 기초한 공원의 개발지표 설정 : 부산광역시 암남공원을 사례로 Case Study of Amnam Park in Busan City

박승범,김승환,남정칠,강영조,이기철,김석규 동아대학교 환경문제연구소 1999 硏究報告 Vol.22 No.2

The purpose of this study is to investigate the prime factors that affect to user postoccupancy evaluation of activities and facilities in urban parks, to classify those factors, to find out factors about them, and then to suggest important indicators in the urban park planning, design, and development. The survey research was done using the self-administered questionnaire method by 435 peoples in the Busan City who participated in recreational activities in the Amnam Park. The Analysis of the data primarily consists of two phase: The first analysis was dealt an exploratory factor analysis which identified major factors involved in user postoccupancy evaluation with reacreational activities and facilities in Amnam Park by the SPSS(Ver.6.0) program and the second analysis was used to investigate the factors affected to user postoccupancy evaluation in the urban park by the Regression Analysis. According to the factor analysis, user postoccupancy evaluation of facilities in urban parks concerning the 20 variables, 5 prime factors by the staistical values were considered. The factor analysis showed that user postoccupancy evaluation of activities in urban parks concerning the 11 variables, 3 prime factors by the staistical values were analysed. Postoccupancy evaluation in the urban park by the Regression Analysis suggested that the two varlables as 'the directions of park development' and 'companies with the personal specific characteristics' are significant in addition to the some variables presented in user postoccupancy evaluation with reacreational activities and facilities in Amnam Park by the factor analysis. Furthomore the analysis models were identified as having significant effects on the factor and regression analysis. Overall fits of both models were very good. These significant relationships indicate important factors and variables that should be considered in planning, design, and development of the urban parks. Therefore, development direction of the h a m park has to be analyzed as significant factors concerning user postoccupancy evaluation in a systematic way, recognized as important variables for planning process. The urban park has to be fully designed and established in the planning process not by the decision-maker's subjective evaluation but by user postoccupancy evaluation.

Choline dehydrogenase interacts with SQSTM1/p62 to recruit LC3 and stimulate mitophagy.

Park, Sungwoo,Choi, Seon-Guk,Yoo, Seung-Min,Son, Jin H,Jung, Yong-Keun Landes Bioscience 2014 AUTOPHAGY Vol.10 No.11

<P>CHDH (choline dehydrogenase) is an enzyme catalyzing the dehydrogenation of choline to betaine aldehyde in mitochondria. Apart from this well-known activity, we report here a pivotal role of CHDH in mitophagy. Knockdown of CHDH expression impairs CCCP-induced mitophagy and PARK2/parkin-mediated clearance of mitochondria in mammalian cells, including HeLa cells and SN4741 dopaminergic neuronal cells. Conversely, overexpression of CHDH accelerates PARK2-mediated mitophagy. CHDH is found on both the outer and inner membranes of mitochondria in resting cells. Interestingly, upon induction of mitophagy, CHDH accumulates on the outer membrane in a mitochondrial potential-dependent manner. We found that CHDH is not a substrate of PARK2 but interacts with SQSTM1 independently of PARK2 to recruit SQSTM1 into depolarized mitochondria. The FB1 domain of CHDH is exposed to the cytosol and is required for the interaction with SQSTM1, and overexpression of the FB1 domain only in cytosol reduces CCCP-induced mitochondrial degradation via competitive interaction with SQSTM1. In addition, CHDH, but not the CHDH FB1 deletion mutant, forms a ternary protein complex with SQSTM1 and MAP1LC3 (LC3), leading to loading of LC3 onto the damaged mitochondria via SQSTM1. Further, CHDH is crucial to the mitophagy induced by MPP+ in SN4741 cells. Overall, our results suggest that CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition.</P>

파트너십에 의한 지하철 입구 공간의 쌈지공원 설계 및 시공

김승환,강현우,손용욱,박승범,남정칠,이기철,차민준 東亞大學校 建設技術硏究所 2009 硏究論文集 Vol.32 No.2

A pocket park is valued in securing green space in downtown and improving urban view in the aspect of natural and environmental values, and it signifies a lot as the center of local residents’ community and harmony as well. Since subway exits and surroundings that have high portions in taking up street space due to lack of green space in downtown aren't made full use of, valuable pocket park place. Dangni Pocket Park of subway exits is a park completed with cooperative works and partnership by students, specialists, administrative organizations, businesses and civilian organizations, etc. without support of special budget. The study site was finally chosen to the space between exit No.1 of Dangni subway station, after analyzing the surrounding circumstances, availability and accessibility of the space among the several subway station exits of Saha, Dangni, and Hadan Station in Busan Metropolitan city. For the designing process, The Citizens organization for Million Amenity Park performed the planning and project proceeding, and 19 design drafts were proposed during the class of landscape architecture in Dong-a University. Design review panel members discussed to select the best draft in practical and authentical, and the related experts reviewed and revised to present the final design plan. The construction was conducted was performed under the cooperation between the civil, authorities, and industrial organizations, among which Saha District office managed for the waste treatment and maintenance matters. The graduates of landscape architecture department of Dong-a University and related industrial enterprises supplied landscape materials and related labors, were supported by 20 members of GPM, one of clubs of Dong-a University volunteered durig the most of construction progress, completing the Pocket Park of subway exits through partnership.

암남공원의 식물상과 삼림식생에 관한 연구

남정칠,박승범,김승환,강영조,이기철,이정아 동아대학교 환경문제연구소 1999 硏究報告 Vol.22 No.1

The objective of this study is to analyze the flora and forest vegetation of Amnam urban natural Park. 10 transects of quadrat of 10×10m were selected for the experiment. They were surveyed from January to October 1997. The obtained results were summerized as follows ; 1. The flora of plants distributed in Amnam Park was 88 families, 245 genus, 374 species. Dicotyledom in an angiosperm was 81.3% of total percentage was 73 families, 194 genus, 304 species, Monocotyledonous plant was 13.9% of total percentage was 7 families, 38 genus, 52 species, Gymnospermous plant was 2.1% of total percentage was 4 families, 5 genus, 8 species. Pteridophyta was 2.4% of total percentage was 3 families. 7 genus, 9 species, Shave grass was 0.3% of total percentage was 1 family, 1 genus, 1 species. 2. The representative wood plants of Amnam Park were Pinus thunbergii, Machilus thunbergii, Camellia japonica, Quercus spp., Celtis sinensis, Morus bombysis, Prunus sangentii, Sorbus alnifolia, Albizzia julibrissin, Juniperus rigida, Platycaya strobilacea, Lespedeza spp., Zanthoxylum piperitum, Zanthoxylum schinifolium, Rhus spp., Carpinus coreana, Mallotus japonicus. Ficus erecta, Pittosporum tobira, Eurya japonica. Elaeagnus macrophylla. 3. The area of plant community in Amnam Park was 84.29% in Pinus thunbergii community. 6.7% in Quercus dentata-Celtis sinensis community, 1.5% in Pittosporum tobira-Eurya japonica community, 1.4% in Quercus aliena-Prunus sargentii community, 1.29% in Pinus thunbergii-Pittosporum tobira community, 1.27% in Pinus thunbergii-Eurya japonica community.

지리산국립공원 심원계곡 생태경관 복원공사지역 식생 변화

정태준 ( Tae-jun Jung ),김연경 ( Yeon-Gyeong Kim ),김영진 ( Young-jin Kim ),정명희 ( Myung-hee Jung ),박경희 ( Kyoung-hee Park ),신창근 ( Chang-keun Shin ),박선홍 ( Seung-hong Park ),김영선 ( Young-sun Kim ) 한국환경생태학회 2021 한국환경생태학회지 Vol.35 No.3

본 연구는 지리산국립공원 심원계곡 생태경관 복원공사지역의 모니터링 결과를 분석하여 체계적인 복원관리를 도모할 수 있는 기초자료를 확보하는데 그 목적이 있다. 복원사업이 완료된 2017년도에 식생 모니터링을 위한 조사구 12개소와 대조구 4개소를 설치하였고, 2017년과 2020년의 상대우점치, 종다양도지수, 유사도 등의 변화를 분석하였다. 복원사업 시 수목이 식재되었던 조사구는 종다양도지수가 0∼1.4552로 나타났고, 대조구와의 유사도지수에서는 1개 조사구가 1.32%로 분석된 것을 제외하고 모두 0%로 나타났다. 조사구의 종다양도지수 및 유사도지수가 매우 낮은 것은 복원사업 시 식재된 수목이 고사하거나 멧돼지 피해, 유수에 의한 침식으로 유실되는 등의 영향으로 판단되었다. 반면 복원사업 시 수목이 식재되지 않은 미식재 조사구는 종다양도지수가 0.9538∼2.3222로 나타났고, 유사도지수는 최대 8.33%까지 분석되었다. 국립공원 생태경관 복원공사지역의 복원 공법 개발을 위한 장기 모니터링과, 미식재 조사구의 천이과정을 분석하는 지속적인 연구가 필요하다. This study aims to obtain basic data for systematic restoration by analyzing the monitoring results of the Shimwon Valley Ecological Landscape Restoration Project area in Jirisan National Park. In 2017, when the restoration project was completed, 12 monitoring plots and 4 control plots were installed for vegetation monitoring, and changes in the relative dominance, species diversity index and similarity between 2017 and 2020 were analyzed. The species diversity index of the surveyed areas where trees were planted during the restoration project was 0-1.4552, and the similarity index with the control group was 0% except for one survey area at 1.32%. The very low species diversity index and similarity index in the survey areas were attributed to the loss of trees planted during the restoration project due to death, damage by wild boars, or erosion by running water. On the other hand, the species diversity index was 0.9538-2.3222 in the monitoring plot where no tree was planted, and the similarity index was analyzed to be as high as 8.33%. It is necessary to continue the long-term monitoring for the development of ecological landscape restoration methods in the national park and analysis of the succession in monitoring plots where no trees were planted.

Method for Protection of Single‑Line‑Ground Fault of Distribution System with DG Using Distance Relay and Directional Relay

Jung‑Hun Lee,Min‑Su Park,Hong‑Seon Ahn,Kyung‑Won Park,Jun‑Seok Oh,Seung‑Gyu Jeon,Dong‑Kyu Kim,Jae‑Eon Kim 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.4

When large scales DG are connected to the distribution system of unidirectional power currents, bi-directional power currents are formed, making it impossible to use conventional overcurrent protection methods alone to detect and isolate fault sections for ground and short-circuit faults. To solve these problems, this study proposed the method of protection against single-line-ground fault of common neutral line multi-contact local power distribution system using distance relay and directional relay. The proposed protection method was applied to the common neutral multi-ground distribution system to validate the protection against single-line-ground fault through the PSCAD/EMTDC software package and efectively detect and isolate only the zone of single-line-ground fault without malfunctions.

관동맥 재개통 후 QTc간격분산의 감소 : 정량적으로 측정한 심근 허혈의 호전 정도와의 연관성 Relationships with the Quantitative Degree of Improvement in Myocardial Ischemia

박상선,최기준,남기병,이철환,홍멸기,박성욱,박승정,신중우,문대혁,김유호 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

골분을 이용한 구리 흡착 첨착

김은정,박정훈,김정섭,유현철,이봉섭,곽명화,우성훈,박승조 東亞大學校 環境問題硏究所 2005 硏究報告 Vol.27 No.1

Spent pig bone was consist of hydroxyapatite. And then we thought that it have a possibility as a adsorbent. The preparation of bone char prepared from spent pig bone in this study. We study on the recycling possibility as a adsorbent is able to adsorb copper ion from their single aqueous solutions. The results are summarized as follows. Bone char that prepared from spent pig bone at 105, 400, 700℃. The adsorption removal efficiency of copper ion onto bone char as adsorbent was above 90% for 15 hours. Increase in the initial pH of the copper ion solution result in an increase in the copper ion uptake per unit weight of the adsorbent. Freundlich isotherm model was found to be applicable for the experiment data of copper ion. The results showed that spent pig bone can be used for the absorption of the copper ion.

반려동물의 기생충검사 이론 및 실제

이성호,박영석,박병권,김상기,정지윤,박수진,박창식,김병수 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

The aim of this article is to improve human health and that of companion animals by improving the diagnosis and control of parasitic diseases. This is achieved by research into parasites of veterinary significance including zoonoses and the use of comparative systems to increase our understanding of some human parasitic diseases. We should promotes learning and teaching through knowledge and operates a diagnostic unit that offers a service to pet owner for indigenous and exotic parasitic diseases of companion animals. We must diagnose and treat them early. Zoonotic parasites are very important because many of animal parasites are zoonoses and give pain and death to human. Parasites are divided Protozoa, Helminthes and Arthropods. There are many protozoa, about 65,000 species. Arthropods are the vector of another diseases, such as virus, bacteria, protozoa and nematodes. Helminthes are composed of Trematoda, Cestoda, Nematoda and Acanthocephala. The target of Veterinary Parasitology is to recognize the characteristics of these parasites and subsequently diagnose, treat and control them. This article is intended to familiarize you with the essential facts and concepts of companion animal parasitology. Emphasis is placed on fecal sample techniques (comparison of fecal examination techniques; frequently found artifacts in fecal smears; direct Smear; zinc sulfate flotation; etc.) We recommend that the study be done to more references on animal parasites.