Combined Gene Therapy with Hypoxia-Inducible Factor-1α and Heme Oxygenase-1 for Therapeutic Angiogenesis

Bhang, Suk Ho,Kim, Ju Hee,Yang, Hee Seok,La, Wan-Geun,Lee, Tae-Jin,Kim, Ga Hee,Kim, Hyun Ah,Lee, Minhyung,Kim, Byung-Soo Mary Ann Liebert 2011 Tissue engineering. Part A Vol.17 No.7

<P>Transfection with either hypoxia-inducible factor-1α (HIF-1α) or heme oxygenase-1 (HO-1) gene can induce neovascularization in ischemic tissues. Although expression of transfected HIF-1α gene occurs rapidly, the expressed HIF-1α protein degrades quickly, limiting its therapeutic efficacy. Meanwhile, expressed HO-1 protein does not rapidly undergo degradation, but gene expression occurs a couple of days after transfection, resulting in apoptosis and a delay in angiogenesis in ischemic tissues at the incipient period of HO-1 gene transfection. We hypothesize that combined delivery of HIF-1α and HO-1 gene will enhance antiapoptosis and neovascularization in ischemic tissue compared with HIF-1α or HO-1 single-gene therapy. To test this hypothesis, ischemic mouse hindlimbs were treated with HIF-1α and/or HO-1 gene therapy. The combined gene therapy proved superior to both single-gene therapies, resulting in rapid expression of HIF-1α gene and long-term maintenance of expressed HO-1 protein. The apoptosis in the ischemic region was significantly less, and angiogenic growth factor secretion and angiogenesis were greater in the combined gene therapy than in either of the single-gene therapies. Our results suggest that a combined gene therapy of HIF-1α and HO-1 enhances the transfection of both genes and improves angiogenesis compared with either single-gene therapy.</P>

복어중독에 의한 가사 상태에서 소생한 1예

송승찬,신진호,강석우,박경남,최호순,박근태,문희식,기춘석,이성희,윤병철,노우균,조균석,이민호 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.3

Tetrodotoxin is a neurotoxin produced by about 90 species of puffer fish and causes paralysis of central nervous system and peripheral nerves by blocking the movement of all monovalent cation. Ingestion of tetrodotoxin produces clinical manifestations such as paresthesias(within 10-45 min), vomiting, lightheadedness, salivation, muscle twitching, dysphagia, difficulty in speaking, convulsion and death that expressed by cardiopulmonary arrest with loss of brain stem reflex sometimes. Tetrodotoxin prevents or delays ischemia induced neuronal death by way of following 3 mechanisms. Firstly, it reduces the energy demand of the brain tissues. Secondly, it delays or even prevents anoxic depolarization. Finally, it deminishes ischemia induced cell swelling and cerebral edema. We report a case of puffer fish poisoning which presented with cardiopulmonary arrest and loss of brain stem reflex, but completely recovered by aggressive cardiopulmonary resuscitation.

冷凍冷藏倉庫 前室部 侵氣防止裝置의 適用에 關한 硏究

석호태,곽현철,송승영,송진규,이영욱 대한건축학회 2003 대한건축학회논문집 Vol.19 No.4

This study aims to evaluate the economic performance of infiltration prevention devices for the refrigerated warehouse. It is showed that the cooling loads of refrigerated warehouses is changed by assembling of infiltration prevention devices in a previous study. Space loads and cooling energy costs according to the types of the infiltration prevention devices are analyzed by the DOE-2.1E simulation. Based on the Life Cycle Costs considered the costs of initial construction, repair·replace, cooling energy and operating energy, the probability of reducing energy consumption and application of infiltration prevent devices in vestibule of refrigerated warehouse is founded.

건물에너지 성능 진단 및 개보수 기법

석호태 영남대학교 공업기술연구소 2001 工業技術硏究所論文集 Vol.29 No.1

In this study, the influence of energy factors on energy consumption in a selected office building is analyzed by sensitivity analysis. Dominant energy factors of each building and system are determined. Then, the energy management schemes for these factors are suggested. If this method is applied to other office buildings, it may be possible to work out a strategy of energy diagnosis and retrofit more efficiently.

인천국제공항 탑승동에 적용 가능한 적정 공조시스템에 관한 연구

석호태,정만석,김지현,여명석,김광우 대한건축학회 2005 대한건축학회논문집 Vol.21 No.1

Generally, indoor thermal environment control consumes much energy in the building Appropriate performance evaluation of the building and HVAC facilities are achieved by an energy simulation The result Which does the energy simulation of property HVAC system in airport Primary, there seems to be reasonable method which save window conduction loads and solar radiation loads on the element for an energy saving Secondly, According to the existence of contact outside in building Loads, the Important factor of influence is divided into solar radiation, window conduction loads and interior loads(lighting, equivalent, occupancy etc) The last, the result which does the energy simulation of each HVAC system, CAV + V AV System is determined profitably in energy performance

법랑모세포 분화와 법랑질 형성과정에서 OD314, Apin protein의 발현 및 기능

박종태,최용석,김흥중,정문진,오현주,신인철,박주철,손호현 대한치과보존학회 2006 Restorative Dentistry & Endodontics Vol.31 No.6

본 연구에서는 법랑모세포 분화와 법랑질 형성에 연관이 있는 OD314 일명 Apin protein의 기능을 밝힐 목적으로, in-situ hybridization에 의한 OD314 mRNA 발현과 법랑모세포 세포주에서 OD314 enamel matrix protein의 발현, 그리고 OD314 유전자를 과발현/억제시킬 수 있는 construct를 제작한 후 법랑질 형성 중에 OD314의 기능을 알아보고자 RT-PCR를 시행하여 다음과 같은 결과를 얻었다. 1. OD314 mRNA는 발생중인 상아모세포보다 법랑모세포에서 강하게 발현되었다. 2. Tuftelin은 석회화 결정이 형성되는 14일까지 발현이 지속되고, 그 이후부터 점차 감소하였다. Amelogenin과enamelin은 7일부터 그 발현이 점점 감소하였다. 3. U6-OD314 siRNA construct를 이용하여 transfection한 법랑모세포 세포주는 OD314와 tuftelin,MMP2 mRNA 발현이 감소하였으며, CM-OD314를 transfection하여 OD314의 과발현을 유도한 경우에는 OD314와 MMP20 mRNA의 발현이 뚜렷이 증대되었다. 이 결과는 OD314가 법랑모세포의 분화와 법랑질의 형성 그리고 석회화 과정에 중요한 역할을 하는 새로운 인자임을 시사한다. This study was aimed to elucidate the biological function of OD314 (Apin protein), which is related to ameloblast differentiation and amelogenesis. Apin protein, calcifying epithelial odontogenic (pindborg) tumors (CEOTs)-associated amyloid, were isolated from CEOTs, and has similar nucleotide sequences to OD314. We examined expression of the OD314 mRNA using in-situ hybridization during tooth development in mice. Expression of OD314 and several enamel matrix proteins were examined in the cultured ameloblast cell line up to 28 days by reverse transcription-polymerase chain reaction (RT-PCR) amplification. After inactivation and over-expression of the OD314 gene in ameloblast cell lines using U6 vector-driven RNA interference and CMV-OD314 construct, RT-PCR were performed to evaluate the effect of the OD314 during amelogenesis. The results were as follows: 1. In in-situ hybridization, OD314 mRNAs were more strongly expressed in ameloblast than odontoblast. 2. When ameloblast cells were cultured in the differentiation and mineralization medium for 28 days, the tuftelin mRNA expression was maintained from the beginning to day 14, and then gradually decreased to day 28. The expressions of amelogenin and enamelin were gradually decreased according to the ameloblast differentiation. 3. Inactivation of OD314 by U6-OD314 siRNA construct down-regulated the expression of OD314, MMP-20, and tuftelin, whereas over-expression of OD314 by CMV-OD314 construct up-regulated the expression of OD314 and MMP-20 without change in tuftelin. These results suggest that OD314 is considered as an ameloblast-enriched gene and may play the important roles in ameloblast differentiation and mineralization.

학교건물의 에너지성능 평가에 관한 연구

정만석,곽현철,석호태 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

Generally, Educational Buildings was selected a direction, plan and scale of building according to the figure of a site. However, In the result. The selection was spent more energy and made every effort to achieve the Thermal Comfort. In the Evaluation of Energy Performance, You must consider whether it will be worthwhile. In this study, we found a difference of Energy Consumption changes according to corridor style, direction, vertical and horizontal location, and roof-space by using DOE-2.1E energy simulation program. Based on this simulation results, found a probability of reducing energy consumption in educational buildings.

고질소 오스테나이트계 스테인리스강에서 Cr₂N 석출상의 결정학적 특성(Ⅱ) : Ⅱ. TEM을 이용한 Cr₂N 결정구조 분석 Ⅱ. TEM Study on the Crystal Structure of Cr₂N

이태호,오창석,이창길,김성준,高木節雄 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.11

In the second part of this study, the crystal structure of Cr₂N precipitates was investigated utilizing transmission electron microscopy (TEM). Based on the analyses of SAD patterns with various zone axes, the crystal structure of Cr₂N was confirmed to be trigonal belonging to the space group P31m, and could be explained in terms of E-type occupational-ordering model. Three sets of superlattice reflections (001), (⅓⅓0) and (⅓⅓1) distinguishing trigonal from hcp were identified in Cr₂N superstructure, and the important characteristics of these reflections were discussed in comparison with the previous studies. (Received August 23, 2004)

정신과에서의 뇌파

전태연,양완석,이경욱,채정호 한국의학사 2004 診斷과治療 Vol.24 No.1

유하 액막과 공기류의 향류 접촉에 있어서 열과 물질이동

김석호,김진환,이우태 전남대학교 촉매연구소 2001 觸媒硏究 論文集 Vol.22 No.-

Heat and mass transfer in the counter-current contact of an air stream and a thin falling liquid film was investigated in a flat-plate wetted wall column. In this study, temperature profiles of the gas phase were measured by micro-thermocouples and composition of gas samples was analyzed by a gas-chromatograph. Local Nusselt numbers were calculated from the temperature profiles. Taking into account the effect of various variables on heat and mass transfer, a new theoretical model was proposed. The model was used to analyze experimental data. Observed heat and diffusion fluxes were compared with theoretical values predicted by a laminar boundary layer theory with high mass transfer at the interface. Good agreement between data and predictions was obtained.