국내 분리 일본뇌염 바이러스 유전자에 대한 항체생성 연구(Ⅰ) : cloning of JEV viral genes

박찬,최우영,이호동,채수림,신은진,김인범,표재환,박근용,조해월 국립보건연구원 1999 국립보건원보 Vol.36 No.-

Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis

Yu, K‐,H.,Hong, K‐,S.,Lee, B‐,C.,Oh, M‐,S.,Cho, Y‐,J.,Koo, J‐,S.,Park, J‐,M.,Bae, H‐,J.,Han, M‐,K.,Ju, Y‐,S.,Kang, D‐,W.,Appelros, P. Blackwell Publishing Ltd 2011 Acta neurologica Scandinavica Vol.123 No.5

<P>Yu K‐H, Hong K‐S, Lee B‐C, Oh M‐S, Cho Y‐J, Koo J‐S, Park J‐M, Bae H‐J, Han M‐K, Ju Y‐S, Kang D‐W, Appelros P, Norrving B, Terent A. Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis. 
Acta Neurol Scand: 2011: 123: 325–331. 
© 2010 John Wiley & Sons A/S.</P><P><B>Background – </B> It has not been clarified whether the disparity in ischemic stroke outcome between populations is caused by ethnic and geographic differences or by variations in case mix. Propensity score matching (PSM) analysis can overcome some analytical problems but is rarely used in stroke outcome research. This study was to compare the ischemic stroke case‐fatality between two PSM cohorts of Sweden and Korea.</P><P><B>Methods – </B> Prognostic variables related to baseline characteristics and stroke care were included in our PSM model. Then, we selected 7675 Swedish and 1220 Korean patients with ischemic stroke from each stroke registers and performed one‐to‐one matching based on propensity scores of each patient.</P><P><B>Results – </B> After PSM, all measured variables were well balanced in 1163 matched subjects, and the 90‐day case‐fatality was identical 6.2% (HR 0.997, 95%CI 0.905–1.099) in Sweden and Korea.</P><P><B>Conclusions – </B> No difference is found in the 90‐day case‐fatality in propensity score‐matched Swedish and Korean patients with ischemic stroke.</P>

Enhanced antitumor immunotherapeutic effect of B-cell-based vaccine transduced with modified adenoviral vector containing type 35 fiber structures

Kim, E-K,Seo, H-S,Chae, M-J,Jeon, I-S,Song, B-Y,Park, Y-J,Ahn, H M,Yun, C-O,Kang, C-Y Macmillan Publishers Limited 2014 Gene therapy Vol.21 No.1

For successful clinical tumor immunotherapy outcomes, strong immune responses against tumor antigens must be generated. Cell-based vaccines compromise one strategy with which to induce appropriate strong immune responses. Previously, we established a natural killer T-cell (NKT) ligand-loaded, adenoviral vector-transduced B-cell-based anticancer cellular vaccine. To enhance tumor antigen delivery to B cells, we established a modified adenoviral vector (Ad-k35) that encoded a truncated form of the breast cancer antigen Her2/neu (Ad-k35HM) in which fiber structure was substituted with adenovirus serotype 35. We observed increased tumor antigen expression with Ad-k35HM in both human and murine B cells. In addition, an Ad-k35HM-transduced B-cell vaccine elicited strong antigen-specific cellular and humoral immune responses that were further enhanced with the additional loading of soluble NKT ligand KBC009. An Ad-k35HM-transduced, KBC009-loaded B-cell vaccine efficiently suppressed the in vivo growth of established tumors in a mouse model. Moreover, the vaccine elicited human leukocyte antigen (HLA)-A2 epitope-specific cytotoxic T-cell responses in B6.Cg (CB)-Tg (HLA-A/H2-D) 2Enge/Jat mice. These findings indicated that the Ad-k35 could be appropriate for the preclinical and clinical development of B-cell-based anticancer immunotherapies.

Microstructure and piezoelectric properties of (Na_0.5K_0.5)NbO₃ lead-free piezoelectric ceramics with V₂O₅ addition

Seo, I.-T.,Park, H.-Y.,Dung, N.V.,Choi, M.-K.,Nahm, S.,Lee, H.-G.,Choi, B.-H. IEEE 2009 and Frequency Control Vol.56 No.11

<P>Various amounts of Nb<SUB>2</SUB>O<SUB>5</SUB> in the (Na<SUB>0.5</SUB>K<SUB>0.5</SUB>) NbO<SUB>3</SUB> (NKN) ceramic were replaced by V<SUB>2</SUB>O<SUB>5</SUB> to decrease its sintering temperature to below 950°C. A small V<SUB>2</SUB>O<SUB>5</SUB> content resulted in a dense microstructure with an increased grain size for the specimen sintered at 900°C due to the presence of a liquid phase. When V<SUB>2</SUB>O<SUB>5</SUB> was added to the NKN ceramics, their orthorhombic-to-tetragonal transition temperature increased from 178°C to around 200°C. However, their Curie temperature decreased from 402°C to around 330°C. The k<SUB>p</SUB>, <SUB>ε3</SUB> <SUP>T</SUP>/<SUB>ε0</SUB>, and Q<SUB>m</SUB> values increased with V<SUB>2</SUB>O<SUB>5</SUB> addition, probably due to the increased density and poling state, which was identified by the phase angle. The specimen with x = 0.05, sintered at 900°C for 8 h, exhibited the following piezoelectric properties: k<SUB>p</SUB> = 0.32, <SUB>ε3</SUB> <SUP>T</SUP>/<SUB>ε0</SUB> = 245, d<SUB>33</SUB> = 120 (pC/N), and Q<SUB>m</SUB> = 232.</P>

한국인 Long Term Non Progressors로부터 분리된 HIV-1 env 및 nef 유전자와 이들의 면역 유전학적 특성 분석에 의한 질병진전 요인 규명

이주실,강춘,김성순,박근용,고운영,기미경,최병선,남정구,서순덕,이성래,구본기,박혜경,도경미,김태규,최희백,조현일,김요숙,김지영,이해정,안수진 국립보건연구원 1999 국립보건원보 Vol.36 No.-

Overview of KSTAR research progress and future plans toward ITER and K-DEMO

Park, H.K.,Choi, M.J.,Hong, S.H.,In, Y.,Jeon, Y.M.,Ko, J.S.,Ko, W.H.,Kwak, J.G.,Kwon, J.M.,Lee, J.,Lee, J.H.,Lee, W.,Nam, Y.B.,Oh, Y.K.,Park, B.H.,Park, J.K.,Park, Y.S.,Wang, S.J.,Yoo, M.,Yoon, S.W.,B IOP 2019 Nuclear fusion Vol.59 No.11

<P>A decade-long operation of the Korean Superconducting Tokamak Advanced Research (KSTAR) has contributed significantly to the operation of superconducting tokamak devices and the advancement of tokamak physics which will be beneficial for the ITER and K-DEMO programs. Even with limited heating capability, various conventional as well as new operating regimes have been explored and have achieved improved performance. As examples, a long pulse high-confinement mode operation with and without an edge-localized mode (ELM) crash was well over 70 and 30 s, respectively. The unique capabilities of KSTAR allowed it to improve the capability of controlling harmful instabilities, and they have been instrumental in uncovering much new physics. The highlights are that the L/H transition threshold power is sensitive to the resonant magnetic perturbation (RMP) and insensitive to non-resonant magnetic perturbation. Co-<I>I</I> <SUB>p</SUB> offset rotation dominated by an electron channel predicted by general neoclassical toroidal viscosity theory was confirmed. Improved heat dispersal in a divertor system using three rows of rotating RMP was demonstrated and predictive control of the ELM-crash with <I>a priori</I> modeling was successfully tested. In magnetohydrodynamic physics, validation of the full reconnection model (i.e. <I>q</I> <SUB>0</SUB>  >  1 right after the sawtooth crash) and self-consistent validation of the anisotropic distribution of turbulence amplitude and flow in the presence of the 2/1 island with theoretical models were achieved. The turbulence amplitude induced by RMP was linearly increased with the slow RMP coil current ramp-up time (i.e. the magnetic diffusion time scale). The <I>D</I> <SUB> <I>α</I> </SUB> spikes (i.e. ELM-crash amplitude) was linearly decreased with the turbulence amplitude and not correlated with the perpendicular electron flow. In the turbulence area, a non-diffusive ‘avalanche’ transport event and the role of a quiescent coherent mode in confinement were studied. To accommodate the anticipation of a higher performance of the KSTAR plasmas with the increased heating powers, a new divertor/internal interface with a full active cooling system will be implemented after a full test of the new heating (neutral beam injection II and electron cyclotron heating) and current drive (CD) (Helicon and lower hybrid CD) systems. An upgrade plan for the internal hardware, heating systems and efficient CD system may allow for a long pulse operation of higher performance plasmas at <I>β</I> <SUB>N</SUB>  >  3.0 with <I>f</I> <SUB>bs</SUB> ~ 0.5 and <I>T</I> <SUB>i</SUB>  >  10 keV.</P>

Study on the Increase of Egg Weight and Early Selection based on Part Record in Layer

Park, Y.H.,Ohh, B. K. The Korean Society of Poultry Science 1983 韓國家禽學會誌 Vol.10 No.2

산란종계의 란중을 개량하기 위하여 란중에 대한 유전력과 유전상관등의 유전적 모수를 추정한 후 백색 레그혼종 2계통(A, K)에서 주령에 따른 란중의 증가양상을 살펴보았다 또한 선발에 의한 유전적 개량량을 크게 하기 위하여 조기선발을 실시할 경우 조기선발의 최적시기를 찾아내는 것이 중요하므로 년 평균란중에 대한 각 기간별 란중의 상관관계와 아울러 회귀관계를 분석하여 최적 선발시기를 알아보았고 각 기간별 선발에 의한 개량량을 상대적으로 나타내므로써 조기선발에 대한 타당성을 구명하고자 본 시험을 수행하였으며 얻어진 결과는 다음과 같다. 주령이 늘어남에 따라 란중도 증가하였는데 산란초기에서는 증가량이 컸지만 점차 감소하였으며 회귀방정식으로 나타낸 증가량은 A, K계통에서 주당 각각 0.46g과 0.83g이었다. 년 평균 란중에 대한 각 기간별 란중의 회귀계수는 29-32주령의 평균란중이 0.86(A), 0.88(K)로 나타나 가장 큰 수치를 보였다. 45-48주령의 평균란중을 기준으로 했을 때의 선발에 대한 각 기간 평균란중의 선발상대효율에서는 A계통에서 33-36주령, K통계에서 29-32주령의 추정치가 가장 크게 나타나서 이 시기에서의 조기선발에, 대한 타당성을 보여주었다. The records on 787 white Leghorn hens from two different strains, A and K were wed in this study to find out the pattern of increase in egg weight with age and the optimum age of early selection for maximizing the genetic gain. The pattern of increase in egg weight with age in two lines which was expressed on graph showed that the amount of increase was larger in the early part of the laying year and it decreased gradually. Regression equations suggested that egg weight increased linearly with age at the rate of 0.46 gram per week for line A and 0.83 gram for line K. And in the regression analyses of each period mean egg weight on annual mean egg weight, the coefficients of EW 30 that is mean egg weight from 29 weeks of age to 32 weeks of age were 0.86 for line A, 0.88 for line K, and those were the highest figures among the all regression coefficients of each mean egg weight on annual mean egg weight. In estimating the relative selection efficiency of each period mean egg weight on annual mean egg weight in EW 46, selection on EW 34 for line A and on EW 30 for line K was the most efficient for improving egg weight. These results suggested that one periods from 29 weeks of age to 36 weeks of age could be used for the best predictable time for improving annual mean egg weight.

ASK1/JNK-mediated TAp63 activation controls the cell survival signal of baicalein-treated EBV-transformed B cells

Park, G. B.,Kim, Y. S.,Lee, H. K.,Yang, J. W.,Kim, D.,Hur, D. Y. Springer Science + Business Media 2016 MOLECULAR AND CELLULAR BIOCHEMISTRY - Vol.412 No.1

<P>Transcriptionally active p63 (TAp63) promotes cell cycle arrest, senescence, and apoptosis in several cancer cells. Migration inhibitory factor (MIF)/CD74 regulates B-cell survival through nuclear factor (NF)-kappa B-dependent TAp63 expression. In this study, we investigated how the level of TAp63 expression influences the induction of apoptosis in baicalein-treated EBV-transformed B cells. Baicalein induced the expression of TAp63 and apoptosis signal-regulating kinase 1 (ASK1), as well as cytotoxicity, by disrupting the mitochondrial membrane and inhibiting the activation of phosphoinositide 3-kinase (PI3K)/Akt and NF-kappa B. Genetic knockdown of TAp63 or ASK1 by small interfering RNA resulted in protection from apoptosis accompanied by the recovery of CD74, CD44, alpha 4 integrin, Bcl-2, and NF-kappa B activation. Baicalein-induced reactive oxygen species activated the ASK1/JNK pathway with subsequent expression of TAp63. Pre-engagement with MIF/CD74 maintained the expression of CD74, CD44, and alpha 4 integrin, as well as Syk/Src-mediated PI3K/Akt activation, in baicalein-treated EBV-transformed B cells. Meanwhile, ASK1/JNK-dependent TAp63 expression was efficiently suppressed after pre-treatment with MIF. Our results suggest that baicalein-mediated ASK1/JNK activation regulates the mitochondria-dependent apoptosis pathway through the up-regulation of TAp63 and downregulation of NF-kappa B and CD74/CD44 in B-cell malignancies.</P>

Novel K/Ka Bandpass Filters using LIGA Micromachined Process

Park, K. Y.,Park, J. Y.,Choi, H. K.,Lee, J.C.,Lee, B.,Kim, J. H.,Kim, N. Y.,Park, J. Y.,Kim, G. H.,Kim, D. W.,Bu, J. U.,Chung, K. W. The Korean Institute of Electromagnetic Engineerin 2000 한국전자파학회논문지 Vol.11 No.6

New class of three dimensional (3-D) micromachined microwave planar filters at K and Ka-band are presented using LIGA micro-machined process. The K-and Ka-band filters show wide bandpass characteristics of~36% and ~39% with the insertion loss 1.26dB at 19.11GHz and 1.7dB at GHz, respectively. These filters can be applicable in high power MMIC of MIMIC.

<i>CYP2A6</i> and <i>ERCC1</i> polymorphisms correlate with efficacy of S-1 plus cisplatin in metastatic gastric cancer patients

Park, S R,Kong, S-Y,Nam, B-H,Choi, I J,Kim, C G,Lee, J Y,Cho, S J,Kim, Y W,Ryu, K W,Lee, J H,Rhee, J,Park, Y-I,Kim, N K Nature Publishing Group 2011 The British journal of cancer Vol.104 No.7

<P><B>Background:</B></P><P>We evaluated the association between polymorphisms of cytochrome P450 2A6 (<I>CYP2A6</I>)/excision repair cross-complementation group 1 (<I>ERCC1</I>)/X-ray repair cross-complementing group 1(<I>XRCC1</I>) and treatment outcomes of metastatic gastric cancer (MGC) patients treated with S-1/cisplatin.</P><P><B>Methods:</B></P><P>Among MGC patients (<I>n</I>=108), who received S-1 (40 mg m<SUP>−2</SUP> b.i.d., days 1–14) and cisplatin (60 mg m<SUP>−2</SUP>, day 1) every 3 weeks, we analysed the wild-type allele (<I>W</I>) and variants (<I>V</I>) of <I>CYP2A6</I> (<I>*4</I>, <I>*7, *9, *10</I>), and the polymorphisms of <I>ERCC1</I> (rs11615, rs3212986) and <I>XRCC1</I> (rs25487).</P><P><B>Results:</B></P><P>Patients having fewer <I>CYP2A6</I> variants had better response rates (<I>W</I>/<I>W vs W</I>/<I>V</I> other than <I>*1/*4 vs V</I>/<I>V</I> or <I>*1/*4</I>=66.7 <I>vs</I> 58.3 <I>vs</I> 32.3% <I>P</I>=0.008), time to progression (TTP) (7.2 <I>vs</I> 6.1 <I>vs</I> 3.5 months, <I>P</I>=0.021), and overall survival (23.2 <I>vs</I> 15.4 <I>vs</I> 12.0 months, <I>P</I>=0.004). <I>ERCC1 19442C</I>><I>A</I> (rs3212986) was also associated with response rate (<I>C/C</I>, 46.7% <I>vs C/A</I>, 55.3% <I>vs A/A</I>, 87.5%) (<I>P</I>=0.048) and TTP (4.4 <I>vs</I> 7.6 <I>vs</I> 7.9 months) (<I>P</I>=0.012). Patients carrying both risk genotypes of <I>CYP2A6</I> (<I>V</I>/<I>V</I> or <I>1/*4</I>) and <I>ERCC1 19442C</I>><I>A</I> (<I>C/C</I>) <I>vs</I> those carrying none showed an adjusted odds ratio of 0.113 (<I>P</I>=0.004) for response, and adjusted hazard ratios of 3.748 (<I>P</I>=0.0001) for TTP and 2.961 (<I>P</I>=0.006) for death.</P><P><B>Conclusion:</B></P><P>Polymorphisms of <I>CYP2A6</I> and <I>ERCC1 19442C</I>><I>A</I> correlated with the efficacy of S-1/cisplatin.</P>