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      • SCISCIESCOPUS

        Microneedle patch delivery to the skin of virus-like particles containing heterologous M2e extracellular domains of influenza virus induces broad heterosubtypic cross-protection

        Kim,,M.C.,Lee,,J.W.,Choi,,H.J.,Lee,,Y.N.,Hwang,,H.S.,Lee,,J.,Kim,,C.,Lee,,J.S.,Montemagno,,C.,Prausnitz,,M.R.,Kang,,S.M. Elsevier Science Publishers 2015 Journal of controlled release Vol.210 No.-

        A broadly cross-protective influenza vaccine that can be administrated by a painless self-immunization method would be a value as a potential universal mass vaccination strategy. This study developed a minimally-invasive microneedle (MN) patch for skin vaccination with virus-like particles containing influenza virus heterologous M2 extracellular (M2e) domains (M2e5x VLPs) as a universal vaccine candidate without adjuvants. The stability of M2e5x VLP-coated microneedles was maintained for 8weeks at room temperature without losing M2e antigenicity and immunogenicity. MN skin immunization induced strong humoral and mucosal M2e antibody responses and conferred cross-protection against heterosubtypic H1N1, H3N2, and H5N1 influenza virus challenges. In addition, M2e5x VLP MN skin vaccination induced T-helper type 1 responses such as IgG2a isotype antibodies and IFN-γ producing cells at higher levels than those by conventional intramuscular injection. These potential immunological and logistic advantages for skin delivery of M2e5x VLP MN vaccines could offer a promising approach to develop an easy-to-administer universal influenza vaccine.

      • SCIESCOPUSKCI등재

        Machine-to-Machine (M2M) Communications in Vehicular Networks

        Booysen,,M.J.,Gilmore,,J.S.,Zeadally,,S.,Rooyen,,G.J.,Van Korean Society for Internet Information 2012 KSII Transactions on Internet and Information Syst Vol.6 No.2

        To address the need for autonomous control of remote and distributed mobile systems, Machine-to-Machine (M2M) communications are rapidly gaining attention from both academia and industry. M2M communications have recently been deployed in smart grid, home networking, health care, and vehicular networking environments. This paper focuses on M2M communications in the vehicular networking context and investigates areas where M2M principles can improve vehicular networking. Since connected vehicles are essentially a network of machines that are communicating, preferably autonomously, vehicular networks can benefit a lot from M2M communications support. The M2M paradigm enhances vehicular networking by supporting large-scale deployment of devices, cross-platform networking, autonomous monitoring and control, visualization of the system and measurements, and security. We also present some of the challenges that still need to be addressed to fully enable M2M support in the vehicular networking environment. Of these, component standardization and data security management are considered to be the most significant challenges.

      • KCI등재후보SCOPUS

        Machine-to-Machine (M2M) Communications in Vehicular Networks

        ( M. J. Booysen ), ( J. S. Gilmore ), ( S. Zeadally ), ( G. J. Van Rooyen ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.2

        To address the need for autonomous control of remote and distributed mobile systems, Machine-to-Machine (M2M) communications are rapidly gaining attention from both academia and industry. M2M communications have recently been deployed in smart grid, home networking, health care, and vehicular networking environments. This paper focuses on M2M communications in the vehicular networking context and investigates areas where M2M principles can improve vehicular networking. Since connected vehicles are essentially a network of machines that are communicating, preferably autonomously, vehicular networks can benefit a lot from M2M communications support. The M2M paradigm enhances vehicular networking by supporting large-scale deployment of devices, cross-platform networking, autonomous monitoring and control, visualization of the system and measurements, and security. We also present some of the challenges that still need to be addressed to fully enable M2M support in the vehicular networking environment. Of these, component standardization and data security management are considered to be the most significant challenges.

      • KCI등재후보SCOPUS

        Effect of Rare Earth Elements on Proliferation and Fatty Acids Accumulation of 3T3-L1 Cells

        M.,L.,He,W.,Z.,Yang,H.,Hidari,W.,A.,Rambeck 아세아·태평양축산학회 2006 Animal Bioscience Vol.19 No.1

        The present study including two experiments was designed to determine the effect of media containing different rare earth elements (REE) on proliferation and fatty acids accumulation in 3T3-L1 cell cultures. In Experiment 1, 3T3-L1 preadipocytes in 96-well plates (1.5횞104 cells/ml) were cultured with Dulbecco`s modified Eagle`s medium (DMEM) containing 10% fetal bovine serum (FBS) for 24 h. Then the media were changed to the following 10 different media for 48 h: DMEM containing 10% FBS for the control; the above media containing 5 쨉M, 10 쨉M or 15 쨉M of LaCl3, CeCl3 or the mixture of these REE chlorides. The proliferation rate of the cells was measured and compared by a non-isotope method-XTT method. In Experiment 2 the cells in 24-well plates (1.5횞104 cells/ml) were cultured in DMEM containing 10% FBS for 7 days until confluent and then were changed to above DMEM containing dexamethasone, methyl-isobutylxanthine and insulin (DMI) for two days. Afterwards the media were changed to the 10 different media with REE supplements as in Experiment 1 and cultured for 6 days. The cells were then harvested for fatty acids analysis by gas chromatography. It was found that supplementation of La (5, 10 and 15 쨉M), Ce (5 쨉M and 15 쨉M) and the mixture REE (5, 10 and 15 쨉M) stimulated (p<0.05) the proliferation of 3T3-L1 preadipocytes (Experiment 1). In the differentiating 3T3-L1 cells supplementation of La (5 쨉M and 10 쨉M), Ce (5 쨉M) and the mixture REE (5 쨉M and 15 쨉M) decreased (p<0.05) the concentration of monounsaturated fatty acids (MUFA) per 105 cells, while the supplementation of La (5 쨉M), Ce (5 쨉M) and the mixture REE (15 쨉M) increased (p<0.05) the ratio of saturated fatty acids (SFA) to MUFA. These results indicate that the supplementation of REE to the media may affect proliferation, differentiation and lipogenesis rates of 3T3-L1 cells. However, the effect may depend upon the level or type of REE applied.

      • Multiple heterologous M2 extracellular domains presented on virus-like particles confer broader and stronger M2 immunity than live influenza A virus infection

        Kim,,M.C.,Lee,,J.S.,Kwon,,Y.M.,O,,E.,Lee,,Y.J.,Choi,,J.G.,Wang,,B.Z.,Compans,,R.W.,Kang,,S.M. Elsevier/North-Holland 2013 Antiviral research Vol.99 No.3

        The influenza M2 ectodomain (M2e) is poorly immunogenic and has some amino acid changes among isolates from different host species. We expressed a tandem repeat construct of heterologous M2e sequences (M2e5x) derived from human, swine, and avian origin influenza A viruses on virus-like particles (M2e5x VLPs) in a membrane-anchored form. Immunization of mice with M2e5x VLPs induced protective antibodies cross-reactive to antigenically different influenza A viruses and conferred cross protection. Anti-M2e antibodies induced by heterologous M2e5x VLPs showed a wider range of cross reactivity to influenza A viruses at higher levels than those by live virus infection, homologous M2e VLPs, or M2e monoclonal antibody 14C2. Fc receptors were found to be important for mediating protection by immune sera from M2e5x VLP vaccination. The present study provides evidence that heterologous recombinant M2e5x VLPs can be more effective in inducing protective M2e immunity than natural virus infection and further supports an approach for developing an effective universal influenza vaccine.

      • SCIESCOPUSKCI등재

        Effect of Rare Earth Elements on Proliferation and Fatty Acids Accumulation of 3T3-L1 Cells

        He,,M.L.,Yang,,W.Z.,Hidari,,H.,Rambeck,,W.A. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.1

        The present study including two experiments was designed to determine the effect of media containing different rare earth elements (REE) on proliferation and fatty acids accumulation in 3T3-L1 cell cultures. In Experiment 1, 3T3-L1 preadipocytes in 96-well plates ($1.5{\times}10^4cells/ml$) were cultured with Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) for 24 h. Then the media were changed to the following 10 different media for 48 h: DMEM containing 10% FBS for the control; the above media containing $5{\mu}M$, $10{\mu}M$ or $15{\mu}M$ of $LaCl_3$, $CeCl_3$ or the mixture of these REE chlorides. The proliferation rate of the cells was measured and compared by a non-isotope method-XTT method. In Experiment 2 the cells in 24-well plates ($1.5{\times}10^4cells/ml$) were cultured in DMEM containing 10% FBS for 7 days until confluent and then were changed to above DMEM containing dexamethasone, methyl-isobutylxanthine and insulin (DMI) for two days. Afterwards the media were changed to the 10 different media with REE supplements as in Experiment 1 and cultured for 6 days. The cells were then harvested for fatty acids analysis by gas chromatography. It was found that supplementation of La (5, 10 and $15{\mu}M$), Ce ($5{\mu}M$ and $15{\mu}M$) and the mixture REE (5, 10 and $15{\mu}M$) stimulated (p<0.05) the proliferation of 3T3-L1 preadipocytes (Experiment 1). In the differentiating 3T3-L1 cells supplementation of La ($5{\mu}M$ and $10{\mu}M$), Ce ($5{\mu}M$) and the mixture REE ($5{\mu}M$ and $15{\mu}M$) decreased (p<0.05) the concentration of monounsaturated fatty acids (MUFA) per $10^5cells$, while the supplementation of La ($5{\mu}M$), Ce ($5{\mu}M$) and the mixture REE ($15{\mu}M$) increased (p<0.05) the ratio of saturated fatty acids (SFA) to MUFA. These results indicate that the supplementation of REE to the media may affect proliferation, differentiation and lipogenesis rates of 3T3-L1 cells. However, the effect may depend upon the level or type of REE applied.

      • SCISCIESCOPUS

        Hydrogen production by auto-thermal reforming of ethanol over nickel catalyst supported on metal oxide-stabilized zirconia

        Youn,,M.H.,Seo,,J.G.,Song,,I.K. Pergamon Press ; Elsevier Science Ltd 2010 International journal of hydrogen energy Vol.35 No.8

        Metal oxide-stabilized mesoporous zirconia supports (M-ZrO<SUB>2</SUB> with different metal oxide stabilizer (M = Zr, Y, La, Ca, and Mg) were prepared by a templating sol-gel method. 20 wt% Ni catalysts supported on M-ZrO<SUB>2</SUB>(M = Zr, Y, La, Ca, and Mg) were then prepared by an incipient wetness impregnation method for use in hydrogen production by auto-thermal reforming of ethanol. The effect of metal oxide stabilizer (M = Zr, Y, La, Ca, and Mg) on the catalytic performance of supported nickel catalysts was investigated. Ni/M-ZrO<SUB>2</SUB>(M = Y, La, Ca, and Mg) catalysts exhibited a higher catalytic performance than Ni/Zr-ZrO<SUB>2</SUB> because surface oxygen vacancy of M-ZrO<SUB>2</SUB>(M = Y, La, Ca, and Mg) and reducibility of Ni/M-ZrO<SUB>2</SUB>(M = Y, La, Ca, and Mg) were enhanced by the addition of lower valent metal cation. Hydrogen yield over Ni/M-ZrO<SUB>2</SUB>(M = Zr, Y, La, Ca, and Mg) catalyst was monotonically increased with increasing both surface oxygen vacancy of M-ZrO<SUB>2</SUB>support and reducibility of Ni/M-ZrO<SUB>2</SUB>catalyst. Among the catalysts tested, Ni catalyst supported on yttria-stabilized mesoporous zirconia (Ni/Y-ZrO<SUB>2</SUB> showed the best catalytic performance.

      • SCISCIESCOPUS

        Characterization of plasmids encoding CTX-M β-lactamase and their addiction systems in Escherichia coli isolates from animals

        Tamang,,M.D.,Gurung,,M.,Kang,,M.S.,Nam,,H.M.,Moon,,D.C.,Jang,,G.C.,Jung,,S.C.,Park,,Y.H.,Lim,,S.K. Elsevier Scientific Pub. Co 2014 Veterinary microbiology Vol.174 No.3

        This study was focused on characterization of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates from chickens and CTX-M associated plasmid addiction systems (PASs) in E. coli from animals using molecular methods. In total, E. coli from nine (9.0%) of the 100 chicken samples examined produced CTX-M type ESBL namely CTX-M-14 (n=4), CTX-M-15 (n=4), and CTX-M-1 (n=1). All of them harbored an additional bla<SUB>TEM-1</SUB> gene. Transfer of bla<SUB>CTX-M</SUB> gene was observed in eight out of the nine bla<SUB>CTX-M</SUB>-positive isolates by conjugation. Plasmid profiling of bla<SUB>CTX-M</SUB>-positive transconjugants revealed a high-molecular weight (95-165kb) plasmid. Pulsed-field gel electrophoresis showed that most CTX-M-producing chicken isolates were genetically diverse. Furthermore, investigation of 92 conjugation-positive E. coli strains carrying bla<SUB>CTX-M</SUB> genes from pigs (n=76), chickens (n=8), and dogs (n=8) identified 230 PASs in the parental strains and 118 in their transconjugants. Among them, hok-sok, pemKI, and pndAC were the most frequently represented PASs in both the parental strains and the transconjugants. Moreover, the hok-sok and pemKI systems were strongly associated to IncF plasmids and the pndAC system to IncI1-Iγ plasmids. Our results suggest that the rapid spread of CTX-M genes in E. coli isolates among the animals could be attributed to the presence of multiple PASs in the CTX-M plasmids. To our knowledge, this is the first report of characterization of CTX-M associated PASs in E. coli isolates from pigs, chickens, and dogs. In addition, CTX-M-1 was detected for the first time in Korea.

      • SCISCIESCOPUS

        Supplemented vaccination with tandem repeat M2e virus-like particles enhances protection against homologous and heterologous HPAI H5 viruses in chickens

        Song,,B.M.,Kang,,H.M.,Lee,,E.K.,Jung,,S.C.,Kim,,M.C.,Lee,,Y.N.,Kang,,S.M.,Lee,,Y.J. Butterworths ; Elsevier Science Ltd 2016 Vaccine Vol.34 No.5

        Highly pathogenic avian influenza (HPAI) H5 viruses derived from A/Goose/Guangdong/1/96 have been continuously circulating globally, severely affecting the public health and poultry industries. The matrix 2 protein ectodomain (M2e) is considered a promising candidate for a universal cross-protective influenza vaccine that provides more effective control over HPAI H5 viruses harboring variant hemagglutinin (HA)-antigens. Here, we evaluated the protective efficacy of a tandem repeat construct of heterologous M2e presented on virus-like particles (M2e5x VLPs) either alone or as a supplement against HPAI H5 viruses in a chicken model. Chickens immunized with M2e5x VLPs alone induced M2e-specific antibodies but were not protected against HPAI H5. The homo- and cross-protective efficacy of M2e5x VLP-supplemented vaccination of chickens was also examined. Importantly, supplementation with M2e5x VLPs induced significantly higher levels of antibodies specific for M2e and different viruses as well as provided improved protection against homologous and heterologous HPAI H5 viruses. Considering the limited efficacy of inactivated vaccines, supplement vaccination with M2e5x VLPs may be an effective measure for preventing outbreaks of HPAI viruses that have the ability to constantly change their antigenic properties in poultry.

      • KCI등재후보

        미세각막절개도 헤드의 선택을 통한 맞춤식각막절개방식의 라식

        강민호,송유미,강성민,박영숙,최철영,이윤정,이병로.Min,Ho,Kang.,M.D..,Yoo,Mi,Song.,M.D..,Sung,Min,Kang.,M.D..,Young,Sook,Park.,M.D..,Chul,Young,Choi.,M.D..,Yoon,Jung,Lee.,M.D..,Byung,Ro,Lee.,M.D. 대한안과학회 2006 대한안과학회지 Vol.47 No.2

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

        Purpose: To compare the intraoperative and postoperative complication rates and visual outcomes with M2 130 head (thick flap group) and M2 110 head (thin flap group). Methods: One-hundred-ninety-five eyes of 104 patients who underwent LASIK with the Moria M2 microkeratome and Allegretto-wave laser were reviewed retrospectively. Selection of M2 heads was based on preoperative pachymetry and estimated ablation depth. Intraoperative and postoperative flap-related complications, mean postoperative uncorrected visual acuity (UCVA), and mean spherical equivalent (MSE) were evaluated and compared. Results: Mean follow-up was 8.1 months. The number of eyes, preoperative MSE and mean corneal thickness of M2 130 were 115, -4.04±1.63diopter (D) and 549.40±39.16??m, and 85, -6.61±3.43D and 525.16±24.53 ?m, respectively, in the 110 head group. Mean UCVA and MSE at postoperative 1 week, 3 months and 6 months were 1.00±0.18, -0.26±0.49D; 1.07±0.68, -0.40±0.51D; and 1.01±0.22, -0.51±0.50D, respectively, in the 130 head group, and 0.90±0.23, -0.46±1.02D; 0.91±0.23, -0.67±0.79D; 0.85±0.46, -0.75±0.88D, respectively, in the 110 head group. There were no significant differences in intraoperative and postoperative flap-related complication rates between the two groups (p=0.316). There were no statistically significant differences in postoperative mean UCVA or MSE between the two groups (p>0.05), except for MSE at the third and sixth postoperative months (p=0.005, 0.013). Conclusions: Proper selection of M2 heads by preoperative pachymetry allowed for an adequate residual stroma bed with good visual outcome. Utilizing one single microkeratome and switching between two heads was advantageous and cost-effective.

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