DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

인공 치아우식 발생 모델에서 디지털 방사선 공제술을 이용한 인접면 치아우식증의 진단

박정훈,최용석,황의환,이기자,최삼진,박영호,김경숙,진현석,홍경원,오범석,박헌국 대한구강악안면방사선학회 2009 Imaging Science in Dentistry Vol.39 No.1

Purpose : The purpose of the experiment was to evaluating the diagnostic ability of dental caries detection using digital subtraction in the artificial caries activity model. Materials and Methods : Digital radiographies of five teeth with 8 proximal surfaces were obtained by CCD sensor (Kodak RVG 6100 using a size #2). The digital radiographic images and subtraction images from artificial proximal caries were examined and interpreted. In this study, we proposed novel caries detection method which could diagnose the dental proximal caries from single digital radiographic image. Results : In artificial caries activity model, the range of lesional depth was 572-1,374 μm and the range of lesional area was 36.95-138.52mm². The lesional depth and the area were significantly increased with demineralization time (p<0.001). Furthermore, the proximal caries detection using digital subtraction radiography showed high detection rate compared to the proximal caries examination using simple digital radiograph. Conclusion : The results demonstrated that the digital subtraction radiography from single radiographic image of artificial caries was highly efficient in the detection of dental caries compared to the data from simple digital radiograph.

Elevational Distribution of Breeding Bird Communities in Seoraksan National Park, Korea

( Hyun-su Hwang ),( Jae-kang Lee ),( Tae-kyung Eom ),( Ho-kyoung Bae ),( Dong-ho Lee ),( Jong-hwan Lim ),( Sung-cheol Jung ),( Chan-ryul Park ),( Shin-jae Rhim ) 한국산림과학회(구 한국임학회) 2020 한국산림과학회지 Vol.109 No.1

In this study, the elevational distribution of breeding birds in Seoraksan National Park, Korea was investigated. Forty-six species of birds were documented from line transect surveys taken from Seorakdong at 230 m above sea level (a.s.l.) to the Daechungbong summit at 1708 m asl. Birdspecies richness and diversity were highest in Seorakdong and lowest at the Daechungbong summit. As elevation increased, bird species richness and diversity decreased, with a humped-shape trend being observed between 700 m and 1200 m a.s.l. Stepwise analyses revealed that breeding bird species diversity indices were significantly negatively correlated with elevation (r² = 0.327, P < 0.001) and positively correlated with vegetational coverage (r² = 0.324, P = 0.046). Higher elevations supported fewer birds than low and intermediate elevations. Projections at the local scale, including data on behavior and habitat use by birds, will be necessary for optimal conservation and management of the bird communities in Seoraksan National Park.

방사선사진의 인덱스-순서 분석을 통한 치아경조직의 탈염화 검출

박동현,박영호,김경숙,박정훈,이기자,최삼진,최용석,황의환,박헌국 대한구강악안면방사선학회 2009 Imaging Science in Dentistry Vol.39 No.2

Purpose : The purpose of this study was to develop the radiographic technique for detecting the demineralization which is known as indication of dental caries. Materials and Methods : This technique was based on the comparing of multiple radiographs which was irradiated by multiple X-ray spectra. For the meaningful comparing, the multiple radiographs were reconstructed to the dosimetrically consistent images using a standard material. The difference of resulting images of same target with multiple spectra represents the difference of response of material as regards the spectra. Results : We have found about 10% of demineralization of dental hard tissues particularly in the proximal region through the analyzing of differences. Conclusion : Most intriguing thing in this investigation was that the method to analyze difference shows us to an anatomic structure of dental hard tissues even if absolute values of optical density were excluded during the procedures.

DJ-1 deficiency impairs synaptic vesicle endocytosis and reavailability at nerve terminals

Kyung, Jae Won,Kim, Jin-Mo,Lee, Wongyoung,Ha, Tae-Young,Cha, Seon-Heui,Chung, Kyung-Hwun,Choi, Dong-Joo,Jou, Ilo,Song, Woo Keun,Joe, Eun-Hye,Kim, Sung Hyun,Park, Sang Myun National Academy of Sciences 2018 Proceedings of the National Academy of Sciences Vol.115 No.7

<P>Mutations in DJ-1 (PARK7) are a known cause of early-onset autosomal recessive Parkinson's disease (PD). Accumulating evidence indicates that abnormalities of synaptic vesicle trafficking underlie the pathophysiological mechanism of PD. In the present study, we explored whether DJ-1 is involved in CNS synaptic function. DJ-1 deficiency impaired synaptic vesicle endocytosis and reavailability without inducing structural alterations in synapses. Familial mutants of DJ-1 (M26I, E64D, and L166P) were unable to rescue defective endocytosis of synaptic vesicles, whereas WT DJ-1 expression completely restored endocytic function in DJ-1 KO neurons. The defective synaptic endocytosis shown in DJ-1 KO neurons may be attributable to alterations in membrane cholesterol level. Thus, DJ-1 appears essential for synaptic vesicle endocytosis and reavailability, and impairment of this function by familial mutants of DJ-1 may be related to the pathogenesis of PD.</P>

때까치(Lanius bucephalus)의 비번식기 먹이꽂이 행동에 관한 연구

박경현(Park, Kyung-hyun),김지연(Kim, Ji-yeon) 한국조류학회II 2020 한국조류학회지 Vol.27 No.1

2003~2009년 10월부터 다음해 2월까지 길동생태공원에서 때까치의 먹이꽂이 212개를 조사한 결과, 11월 중순부터 먹이꽂이가 급격히 증가하여 추위가 본격화되기 전 먹이를 저장함을 알 수 있었다. 먹이꽂이에 이용된 동물은 메뚜기목과 양서강이 가장 많았고, 그 외 절지, 연체, 환형, 척추동물문의 다양한 먹이를 겨울철 먹이로 이용하였고, 2개월 내에 70%가량이 없어졌다. 먹이꽂이 높이는 평균 131.5cm로, 아까시나무와 버드나무를 주로 이용하였으나, 그 외 다양한 수종도 이용하였다. 수종보다는 먹이를 꽂기 쉬운 높이의 수목 중 DBH 3cm미만인 교목을 선호하였다. 먹이꽂이와 관찰로의 거리를 조사한 결과, 관찰로 주변을 더 선호하였다. 길동생태공원에서 비번식기에 서식하는 때까치의 먹이꽂이 행동에 관한 연구 결과, 도시 비오톱에서 때까치의 비번식기 서식을 위하여 먹이원이 되는 곤충과 양서류 등 종다양성을 높게 유지하는 것이 필요하며, 일정한 거리 내에 습지와 개방된 초지, 산림이 어우러진 수직적 자연경관을 유지해야 함을 알 수 있었다. 2003~2009 from every October to following February(7 years), we have observed 212 impaled preys of Lanius bucephalus in Gildong Ecological Park. As a result, we got to know that the number of impaled preys sharply increased from the middle of November in every year, and the food storage of preys has been completed before severely cold time. The main preys used for its impalement were orthoptera and amphibia. Besides, various preys such as arthropoda, mollusca, annelida and vertebrate were also used as impaled food for winter time. And 70% of its cache of preys disappeared within 2 months. The impalement location on a tree was 131.5cm high of trees on average. And then it mainly used Salix koreensis and Robinia pseudoacacia for impalement but it also used various other trees. It preferred the trees of which DBH(Diameter at Breast Height) is less then 3cm among the trees of easy-to-impale height rather than wood species. According to examining the distance between impalement and observation road, it is recognized that Lanius bucephalus liked the surrounding area of observation road. As a result of this study on impaling behavior of Lanius bucephalus in non-breeding season in Gildong Ecological Park, for the inhabitation of Lanius bucephalus in non-breeding season at the biotop in urban area, we got to know it is required that biodiversity on food source such as insect and amphibian etc. should be kept in a high level, and also within a certain boundary, vertical natural landscape which combines wetlands, open pastures and forest should be maintained.

Elevational Distribution of Breeding Bird Communities in Seoraksan National Park, Korea

Hwang, Hyun-Su,Lee, Jae-Kang,Eom, Tae-Kyung,Bae, Ho-Kyoung,Lee, Dong-Ho,Lim, Jong-Hwan,Jung, Sung-Cheol,Park, Chan-Ryul,Rhim, Shin-Jae Korean Society of Forest Science 2020 한국산림과학회지 Vol.109 No.1

In this study, the elevational distribution of breeding birds in Seoraksan National Park, Korea was investigated. Forty-six species of birds were documented from line transect surveys taken from Seorakdong at 230 m above sea level (a.s.l.) to the Daechungbong summit at 1708 m asl. Birdspecies richness and diversity were highest in Seorakdong and lowest at the Daechungbong summit. As elevation increased, bird species richness and diversity decreased, with a humped-shape trend being observed between 700 m and 1200 m a.s.l. Stepwise analyses revealed that breeding bird species diversity indices were significantly negatively correlated with elevation (r² = 0.327, P < 0.001) and positively correlated with vegetational coverage (r² = 0.324, P = 0.046). Higher elevations supported fewer birds than low and intermediate elevations. Projections at the local scale, including data on behavior and habitat use by birds, will be necessary for optimal conservation and management of the bird communities in Seoraksan National Park.

문학,문화 : 디지털 시대 도시의 생태학적 전망

이경래 ( Kyung Lae Lee ),박규현 ( Kyou Hyun Park ),조연정 ( Yeon Jung Cho ) 경희대학교 비교문화연구소 2012 비교문화연구 Vol.26 No.-

City`s Ecological Landscape in the Digital Age Lee Kyung-Lae, Park Kyou-Hyun, Cho Yeon-Jung We all know how beautiful our wild and it`s importance to our living planet Earth. But did you realise the speed at which man himself is damaging it`s unique natural habitat. We are well on our way to destroy our forests, plants, wetlands. We are polluting our oceans and seas. This way, we`re driving numerous animal species, plant species and many others into extinction. Everyone should be aware of the importance of our natural environment. We live in the period of echocide. Why we need nature to survive and how we can deal with the environmental problems we face. This paper has the purpose to reform city`s environment. Because, Metropolis and megalopolis are the principal cause of environmental disruption. To reform the city is needed to consider digital technology in our age. In the face of economic and cultural globalization, many have argued that we live an increasingly placeless world. However, as a growing number of cities participate and compete in key marketplaces of advanced capitalism, the spectacle of the city is more than ever a significant medium of communication in its own right. In doing so, this work is focused specifically on the dimension of city`s media environment. To that end, the paper examined U-City and U-Eco city. In this study, we will introduce the study on model of U-Eco City as one way for the eco-freindly future city.

공무원들의 인식에 나타난 경관자원 특성 분석 연구

박선명(Park Sun-Myung),박선경(Park Sun-Kyung),김주현(Kim Joo-Hyun),김종하(Kim Jong-Ha),이정호(Lee Jung-Ho),최무혁(Choi Moo-Hyuck) 대한건축학회 2010 대한건축학회논문집 Vol.26 No.11

In this study, to set up landscaping plans, characteristics of landscape were extracted based on opportunity and weaknesses factors of landscape after carrying out a questionnaire targeting officers in 23 cities and counties in Kyung-buk province and the findings are as following. First, in analyzing condition, component and value of landscape based on opportunity and weaknesses factors, for opportunity factors, it was found that the condition of landscape was quantitatively great, the component of landscape was industrially great and the value of landscape was great in possibility of development. Also, for weaknesses factors, the 'quality' was superior in its condition, the industry was superior in its 'component' and the shortage of artificial resources was superior in its 'value'. Secondly, in the relation between factors consisting opportunity and weaknesses resources, firstly, it was found that industry, city and nature are superior for opportunity factors, and culture, traffic and population are superior for weaknesses factors. Next, in the relation between component and condition, 'qualitatively superior industry' and 'quantitatively superior industry' had the highest frequencies in opportunity factors and 'qualitatively outstanding city' and 'quantitatively preponderant industry' had the highest frequencies in weaknesses factors. Thirdly, in analyzing the importance of opportunity and weaknesses factors, for opportunity factors, qualitatively superior resource was a priority in the importance of condition and for weaknesses factors, it was qualitatively little greater than the quantity. In the importance of component, the industry was most superior in both of strength and weak points and in the importance of value, the shortage of artificial resources was a priority. Fourthly, in characteristics of landscape, internal characteristics were found stronger than external characteristics in each city and county. This means that there are more resources to strategize in landscape plans of individual city and county unit than other in metropolitan unit. Also, possibility of development, possession of complex resources and geographical adjoin were common points of their strategies and plans.

NSIP와 BOOP의 고해상전산화단층촬영 소견과 비교

백상현,박재성,차장규,홍현숙,김대호,박성진,조준희,이혜경,최진수 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.2

Purpose: BOOP and NSIP also share similar clinical features such as subacute flu-like illness, the finding of bronchoalveolar lavage, the result of pulmonary function test and more favourable outcome than usual interstitial pneumonia(UIP). The author try to compare the HRCT findings of NSIP with BOOP by pattern and distribution of diseases. Materials and Methods: HRCT findings of histopathologically proven NSIP(Group Ⅰ; 6, Group Ⅱ; 7, Group Ⅲ; 2) in 15 patients(5 male, 10 female, age range, 39-69 years; mean age, 53 years), BOOP in 15 patients(6 male, 9 female, age range, 26-76 years; mean age, 52 years) were reviewed retrospectively. Results: The common pattern of the two diseases was admixed consolidation, ground glass attenuation & reticular densities with/without architectural distortion(NSIP; 53.3%, BOOP; 46.7%). The main component of the pattern wan predominantly consolidation(NSIP; 46.7%, BOOP; 73.3%) and ground glass attenuation(NSIP; 40.0%, BOOP;20.0%). Architectural distotion was more provinent finding with NSIP(mild ; 3, moderate ; 4, severe; 2) compared with BOOP(mild; 7, moderate; 2, severe; 0). The predominant overall distribution was lower lung, peribronchovascular & peripheral lung field in both of the two entities(NSIP;73.3%, BOOP; 73.3%). Peribronchovascular involvement was more common with NSIP(100%) than BOOP(80%), whereas peripheral involvement was more common with BOOP(93.3%) than NSIP(80%). Conclusion: The HRCT findings of NSIP and BOOP were similar in pattern and distribution. But NIP manifested more frequently ground-glass attenuation, whereas BOOP showed more frequently consolidation. Architectural distortion was more severe with NSIP than BOOP. NSIP invoved more frequently peribronchovascular space and BOOP were more common in peripheral lung field.