소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

Pulse Oximeter를 이용한 치수생활력측정

이제호,구본경,이종갑 大韓小兒齒科學會 2000 大韓小兒齒科學會誌 Vol.27 No.1

치수 생활력 검사시 전통적인 방법으로 전기치수검사나 온도변화검사 등이 있다. EPT와 ice test 즉, 전기치수검사와 온도변화검사는 치아의 신경학적 반응에 의해 치아의 실활여부를 판단하는 방법으로 환자가 아동일 때는 정확한 반응을 얻기가 어렵고, 환자의 주관적 반응을 판단해야 하므로 객관적이지 못하고, 소아환자에게 좋지 못한 자극을 주어 행동 조절의 문제를 일으키며, 거짓 양성반응과 거짓 음성반응이 나올 수 있다는 등의 한계가 있다. 최근에는 전통적인 방법의 한계를 극복하려는 시도에서 혈관의 보전성을 평가하는 방법인 laser doppler flowmetry와 pulse oximeter를 이용하는 방법이 소개되고 있다. Pulse oximeter의 원리는 두 가지 종류의 파장의 빛을 귀, 손가락 등 생체의 말단에 투과시켜 발산된 빛과 감지된 빛간의 두 파장의 흡수비로 산소포화 정도를 알아내는 것으로서 이에 착안하여 또 하나의 생체 말단인 치아에 이를 적용하여 치아의 실활 여부를 판단하는 것이다. 이 보고에서는 치수 생활력 검사시 pulse oximeter의 사용 가능성에 대해 검증하고 이의 임상적용에 대해 기초적 자료를 제공하고자 함을 목적으로 했는데 생활치에서는 평균 96.3%의 산소 포화도를 실활치에서는 평균 0.0%의 산소 포화도를 얻어냄으로서 pulse oximeter가 치아의 실활여부 판단에 있어 유용한 진단도구로서의 가치가 있다는 것이 밝혀졌다. Traditionally, EPT and thermal tests were used as diagnostic methods for pulp vitality test. The thermal and electrical stimulation tests are the methods to determine the vitality of a tooth based on its neuronal response. These have certain limitations, one of them is the difficulty of approaching the correct result in case of treatment of children. The reason is management problem caused by the unpleasant stimulation. Also, the response from patients are not objective, and false positive or false negative could be happened. Recently, laser doppler flowmetry and pulse oximeter which evaluate vascular integrity are introduced-in an effort of overcoming to limitation of traditional methods. The principle of pulse oximeter is to find out level of oxygen saturation by ratio of the two pulses between emitted light and detected light penetrating them to the termination of body, such as ears or fingers. From this point of view, it can be applied to a tooth to determine its vitality. The objective of this study lies mainly on varifying pulse oximeter as a method of determining tooth vitality and providing basic data of its clinical implementation. The result of the research showed that level of oxygen saturation in vital teeth was average of 96.3% and 0.0% in pulpless teeth. As a comprehensive result, pulse oximeter could be an useful diagnostic equipment in determining of tooth vitality.

農業法人 經營體의 運營實態와 育成方向

이기웅,박종구 순천대학교 지역개발연구소 1998 地域開發硏究 Vol.9 No.-

In order to deal with the rapidly changing environment such as trade liberalization and the problem of local autonomy, the Korean government has implemented a comprehensive master policy entitled, "Agricultural Development Plans". It especially focuses on improving the agricultural structure and enhancing the farmers' living standard. In 1994, the government issued a new plan "Agriculture and Fisheries Development Plan(AFDP)". Increasing concern from the rural dwellers about the future of their life has made the government establish new policy, focusing on more welfare-oriented program. These changes and other pressures indicate that agriculture should move in the direction of less cost, improved efficiency, transparency and consistency, and market-orientation. Most have recommended general and widespread reform of agricultural policies based on market-oriented direction with competitiveness in world market. The purposes of this study are to identify the operating situation of farming corporation and to suggest future direction to improve the agricultural structure so that it meets the requirements of a competitive agriculture under the WTO system.

한약재가 Aspergillus parasiticus R-716의 生育과 aflatoxin 生成에 미치는 影響

구성회,이용욱,정덕화,김종규 한국식품위생학회 1988 식품위생학회지 Vol.3 No.2

한의원에서 오래 前부터 사용되어 오고 있는 몇 종의 한약재를 구입하여 이들을 chloroform으로 추출하여 추출물을 조제한 다음 공시균(Aspergillus parasiticus R-716)의 生育과 aflatoxin 生成에 미치는 影響을 조사한 결과 다음과 같은 結論을 얻었다. 1. 목단 추출물 첨가군에서 가장 현저하게 공시균의 生育이 저해되었고 그외의 첨가군에서도 강황, 봉출, 향부자, 백작액, 백출의 順으로 저해되었다. 2. Aflatoxin 生成은 백출과 강황 추출물 첨가군에서만 저해되었고, 나머지 첨가군에서는 오히려 증가되거나 효과가 적었다. 3. 백출 추출물 0.2ml첨가시 3일째부터 菌體 生成을 시작하여 9일째에 0.953g/30ml이었고, aflatoxin 함량은 792㎍으로서 대조군에 비해 약 50%가 감소되었다. 菌體 g當 aflatoxin 함량은 992㎍으로서 대조군의 1,467㎍에 비해 역시 크게 감소되었으며 NADPH oxidase 活性은 대조군보다 높아 백출 추출물이 공시균의 生育과 aflatoxin 生成에 저해 효과가 있는 것으로 나타났다. The possible effects of some oriental herbs, which have been used to treat cancerlike disease in Korea, on growth and aflatoxin production by Aspergillus parasiticus R-716 were investigated. Zedoaria rhizoma, Curcuma longa, Cyperus rotundus, Angelica gigas, Paeonia albiflora, Paeonia mountan, Atractylis ovata, and Pulsatilla koreana were extracted with chloroform. Among them the extract of Paeonia mountan was remarkably effective on the growth inhibition, and Curcuma longa, Zedoaria rhizoma, Cyperus rotundus, Paeonia albiflora, Atractylis ovata also inhibited the growth. The extract of Atractylis ovata and Curcuma longa, also inhibited the aflatoxin production but the others showed no effect at all or sometimes stimulated effect. With the addition of 0.2ml extract of Atractylis ovata in 30ml SLS medium, the growth was delayed for about 2 days, and after 9 days, mycelium weight was 0.953g, and total aflatoxin was reduced 50%(792㎍)of that produced in the control(1547㎍). Aflatoxin per mycelium weight was decreased 32%(992㎍)of that produced in the control(1467㎍), but NADPH oxidase was higher as compared to the control. The extract of Atractylis ovata appeared to have a inhibitory effect on the growth and aflatoxin production of Aspergillus parasiticus R-716.

市·郡 統合型 地域農業의 組織化에 관한 硏究

이기웅,박종구 순천대학교 지역개발연구소 1997 地域開發硏究 Vol.8 No.-

Every circumstance surrounding Korean agriculture is getting worse and unfavorable. First of all, in view of domestic situation, Korean agriculture faces widespread small scale farming, greater proportion of an old labor force, rice monoculture, stagnant and neglible investments, and the poor infrastructure in support of agricultural production. Thus, the agricultural and rural areas remain underdeveloped with low productivity. In addition to the challenging internal problems of agriculture, the agricultural industry is not internationally competitive and not well prepared for the market liberalization. It is clear that, under open international competition, many low productivity farms would be forced out of business, and the resulting social cost and unrest would be significant. In this point of view Korean government has enforced urban-rural integration Under these considerations, the main objectives of this paper are 1) to analyze the regional agricultural structure of urban-rural integration(Sunchon City) and 2) to find out the organization direction of regional agriculture to urban-rural integration. In conclusion, in order to organize regional agriculture to urban-rural integration, we should make all-out efforts such as Production-Pursuiting Type, Marketing-Oriented Type, and Regional Self-Supporting Type.

超音速流(M∞=4.5)속에 놓인 Wedge 周邊의 密度分布

李鍾憲,田龜濟 東亞大學校 1966 東亞論叢 Vol.3 No.-

本 硏究는 擴散型衝擊波管(Divergent Shock tube)에 依해서 만들어진 超音速流(M∞=4.5, R∞=1.1×??/cm) 속에 놓인 平面 Wedge 周邊의 密度分布를 Mach-Zehender 干涉計를 使用하여 Magnesium 擊發光源(4481Å)에 依한 干涉무늬를 寫眞으로 瞬間撮影하여 決定하는 것이다. Density distribution of the Supersonic flow past a plane wedge was determined by an optical method. Raynolds number of the Uniform flow which is generated by the shock tube is R∞=1.1×?? per centimeter and the Mach number is M∞=4.5. As an optical method the Mach-Zehender Interferometer was used mainly and the wave length of the light source is 4481Å utilizing the magnesium spark.

양단힌지 비대칭 포물선아치의 자유진동에 관한 연구

이병구,남궁문,김종만 圓光大學校 1986 論文集 Vol.20 No.2

양단힌지 비대칭 포물선아치의 자유진동을 지배하는 미분방정식가 경계조건을 D'Alembert원리와 평형방정식을 이용하여 유도하였다. 유도된 미분방정식에는 회전관성의 영향을 고려하였다. 위의 미분방벙식을 수치적분하기 위하여 Runge-Kutta Method가 이용되었으며, 고유 진동수 매개변수의 값F를 찾기 위하여 시행착오적 고유치문제가 이용되었다. 상세연구로는 양단힌지 포물선아치의 최저차의 3개의 고유진동수를 해석하였다. 회전관성이 고유진동수에 미치는 영향은 무시할 정도로 작았다. 또한 수치해석의 결과로 고유진동수-아치 지간길이의 관계를 그림에 나타내었다. The governing differential equations and the boundary conditions for the free vibration of hinged ended unsymmetric parabolic arch are derived on the basis of the equilibrium equations and the D'Alembert principle. The effect of rotary inertia as well as extentional and flexural deformations is considered in governing differential equations. A trial eigen value method is used for determining the natural frequencies. The Runge-Kutta method is used in this method to perform the integration of the differential equations. The detailed studies are made of the lowest three vibration frequencies for the hinged ended unsymmetric parabolic arches. The effect of the rotary inertia is analyzed and it's numerical data are presented in table. And as the numerical result the frequency versus the span length of arch is presented in figure.

강의 현대화 추진 및 활용방안 연구

구경완,이근복,장종국,김재홍,김영환,최제영,이승훈 永同大學校 1997 硏究論叢 Vol.3 No.1

본 연구는 각종 첨단 기자재를 활용한 강의 현대화 방안을 모색함으로서 공학교육의 효율성을 도모하고 학생들의 학습동기를 유발하여 창조적 능력을 갖츤 전문기술 인력을 양성하는 데 도움을 주고자 하는 것이다. 이를 위하여 현행 공학교육의 현황과 문제점을 분석하고 강의 현대화 사례를 조사하여 본 연구의 기초자료로 삼고자 하였으며, 학부·전공별 강의 현대화 추진 실태분석 및 개선방안을 모색한 후 이상의 연구결과를 종합하여 향후의 강의 형대화 추진방향을 걸정하였다. This study aims to help bring up the well-skilled and creative engineers by searching the modernization of education using various high technology facilities. For this, we analyzed the general condition and problems of current engineering education and surveyed the case study of the modernization of education as basic study. After analyzing the existing situation of promotion searching the proper improvement methods of it, we proposed the new direction of the modernization of education from now on.

섬유상 담체 종류에 따른 생물학적 오수처리 비교연구

이수구,임병란,한종옥 서울産業大學校 1994 논문집 Vol.40 No.1

This study was performed to investigate the treatability of sewage from department store by attached growth biological treatment method using several fibrous media such as Supermodule made of polyvinylidine chloride and acry fiber(FBC), ringlace made of polyvinylidine chloride(HBC) and polypropylene(PP). The pilot plant was set up in the field of K department store and four consecutive aerated reactors were packed with each media and maintained in the condition of hydraulic retention time 12 hours. Experimental results showed that the effluent BOD concentration was stable even though the influent concentration was largely fluctuated in the range of 300~900㎎/L. BOD removal efficiencies in HBC, PP and FBC systems were 90%, 87% and 94%, respectively. The influent BOD was mainly removed in the first, second and third reactors and the removal rate in the fourth reactor was the lowest due to the low concentration and low influent load. The concentration of biomass attached to fibrous media was the highest in the FBC system compared with HBC and PP systems. Total concentration of volatile solids in the FBC system was 5,900 ㎎/L which was high compared with the other media systems. The production ratios of waste sludge were 9.2, 8.45 and 7.3 gDS/㎥ of influent sewage in HBC, PP and FBC systems, respectively.

성인 급성 신부전의 원인 및 예후인자 분석

구영선,장윤경,양종오,강민규,황평주,김종학,나기량,이강욱,신영태 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Acute renal failure is a frequent complication in hospitalized patients and is strongly related to the mortality. The Clinical outcome and prognostic factors of acute renal failure(ARF) have been analyzed by many authors. The present study describes the etiologic and clinical aspects, as well as other factors related to mortality. All the patients suffering from acute renal failure admitted during the period of January 1993 - August 1998 were included in the study. The average age of the patients was 52±17 years and mortality rate was 24%. The causes of acute renal failure were hemorrhagic fever with renal syndrome(HFRS), sepsis, renal hypoperfusion, urinary tract obstruction, acute tubular necrosis, etc. The etiology of ARF was a significant prognostic factor on mortality in ARF. Other significant prognostic factors were oliguria, organ failure, use of vasoconstrictors, hypotension, serum bicarbonate, premorbid conditions, sepsis, neurologic complications, gastrointestinal bleeding. On the other hands, operation, sex, anemia, thrombocytopenia, hypoalbuminemia, BUN, serum creatinine, and hyperkalemia were not significant factors for the mortality. We conclude that major prognostic factors of acute renal failure arc premorbid conditions, sepsis and multiorgan failure, and they are responsible for persistent high mortality of acute renal failure despite of advances of medical care.