Anti-Obesity Effect of Auricularia spp.

Kun Hee Park,Kil-ja Kim,Kab Yeul Jang,Kimoon Park 한국버섯학회 2017 버섯 Vol.21 No.2

The purpose of this study is to find out a food material having anti-obesity effect and suggest the effect of the material to people who are interested in anti-obesity. For this study, Auricularia (wood ear) has been used. It is world widely spread mushroom, especially in east north Asia countries. It is also called as wood ear in western countries because of its shape. The most considerable fact is that Auricularia is being used for popular food materials for cooking because of its good taste. Totally 11 strains of Auricularia were offered by Doctor Kim, Kil-ja in Jeollanam do Agricultural Research & Extension Services. There were 8 strains of Auricula-polytricha, and 3 strains of Auricula-judae. Each Auricularia strains were extracted with 50% Ethanol for 12 hours in 40°C and then 11 strains were prepared for in vitro study. In vitro study focused on the inhibition effect on adipocyte differentiation of Auricularia spp. 3T3-L1 (preadipocyte cell) was used for identifying the inhibition effect on adipocyte differentiation. Before that, MTT assay was used for measurement of cell viability. The result is essential for fixing the treat concentration of the extracts. After adipocyte differentiation, cells were stained with Oil Red O solution to dye differentiated adipocyte cells. Spectrophotometer was used to quantify the adipocyte cells. There are few adipocyte differentiation markers. From the markers, this study selected four markers (PPAR γ, FABP4(ap2), CD36, C/EBPα) and used them to measure mRNA expression using real-time PCR and Western blot experiment. As a result, this study found out that all the extract of strains has inhibition effects on adipocyte differentiation, especially the adipocyte differentiation control probability of Auricula-polytricha strain 21001 was 24.6%. Auricula-judae strain 21002 (19.3%), Auricula-polytricha strain 249 (14.0%) followed. The result concerning the inhibition effect was identified through Western blot experiment as well. As a further study, in vivo study is on progressing. 56 ICR mice (6w, male) are being used for the animal study. It consists of 8 groups. One is control, another is positive control, the other groups are Auricula-polytricha strain 21001 treatment group. Different concentration of powder or extract of Auricularia is treated in each treatment group. We are anticipating that the result of this further study will be correspond to that of in vitro study.

Pascal의 Pensees에 나타난 悲慘과 偉大의 意味

金吉子 대구효성가톨릭대학교 1980 연구논문집 Vol.22 No.1

급성 및 만성 간질환에서 C형 간염바이러스 항체(anti-HCV)양성률

김삼용,성자원,김병호,이기천,허승식,길준영,정현용,이헌영,김영건 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

The prevalence of antibody against hepatitis C virus (anti-HCV) was investigated in 331 patients with various liver diseases from April 1992 to September 1992. The presence of anti-HCV was detected by Lucky HCD EIA test in 331 cases and by (Ortho HCV Antibody ELISA test 148 patients) The results are summarized as follows: 1. Overall, antibody to HCV(anti-HCV) was positive in 37(11%) of 331 patients with various liver diseases. 2. 16(11%) of 144 patients with chronic hepatitis, 2(5%) of 37 patients with alcoholic liver disease, 17(22%) of 76 patients with liver cirrhosis and 2(5%) of 38 HBsAg carriers were positive for anti-HCV.None of 12 patients with acute hepatitis, 11 patients with drug induced hepatitis and 13 patients with hepatocellular carcinoma was anti-HCV positive. 3. HBsAg positivity in patients who had anti-HCV was 24%(9/37). 4. A positive correlation was found between Lucky HCD and Ortho HCV test. Of 128 Lucky HCD negative cases, 124 cases were negative for Ortho HCV ELISA and 4 cases were positive for Ortho HCV ELISA Of 20 Lucky HCD positive cases, 11 cases were positive for Ortho HCV ELISA and 9 cases were negative for Ortho HCV ELISA. These results suggest that hepatitis C virus has an important etiologic role in HBsAg negative chronic hepatitis and liver cirrhosis in Korea. The diagnostic value of Lucky HCD EIA test may be slightly better or equivalent to Ortho HCV ELISA test in the diagnosis of Hepatitis C virus infection.

서울뚝섬 近郊農業의 變容

金吉子,裵瑛奎,許淑烈 梨花女子大學校 師範大學 社會生活科 1962 綠友硏究論集 Vol.- No.4

鹽油中 呈味成分의 檢索

金香仙,金賢榮,朴吉子,劉炳淑,李揆媛,洪璟珠 梨花女子大學校 藥學硏究所 1961 梨花藥學硏究 Vol.- No.2

녹차의 주성분인 에피갈로카테킨 갈레이트의 정맥주사후 흰쥐에서의 약물속도론 연구

임재수,황성주,지웅길,김동출 충남대학교 약학대학 의약품개발연구소 2000 藥學論文集 Vol.16 No.-

The pharmcacokinetic study of epigallocatechin gallate was performed following iv bolus administration in Sprague-Dawley rats. The values of systemic clearance (CL) were 68±5 and 27±1 ml/min/kg following iv bolus administration of 1 mg and 5 mg EGCG, respectively. The values of volume of distribution at steady state were 380 and 835 ml/kg body weight after iv bolus administration 1mg and 5mg EGCG, respectively. The decrease in the value of CL and the increase of Vss as a function of dose suggest saturable pharmacokinetics of EGCG in rats.

間歇的 運動에 의한 脫水 및 水分攝取의 生理的 效果

朴哲浩,朴吉俊,李相于,金永明,朴贊熙,玄松子,呂南會,朴相甲,金榮俊 東亞大學校附設스포츠科學硏究所 1989 스포츠科學硏究論文集 Vol.7 No.-

This study aims at revealing the physiological responses of dehydration (DH) and rehydration (RH) in intermittent exercises at the work intensity of 70% HR max. First, with general student public, three times of exercise (each for 20 minutes) at the given work intensity were taken, with a break for 25 minutes respectively. Their heart rate, rectal temperature and plasma electrolyte concentration both DH and RH were measured. Second, in two groups of athletes and non-athletes, 9 rounds of exercise (for 100 minutes) and 8 breaks (for 60 minutes) were taken, with RH forced 10 times to compensate for the subjects' water loss. Their change in sweat loss and plasma electrolyte concentration as well as the IVDP(Intensive Voluntary dehydration Phenomenon) were experimented. The summary of this research follows. 1) Mean heart rate and rectal temperature in intermittent exercises were higher at DH than at RH, growing up at the increasing level of DH. 2) RH saw no change in the plasma concentration of Na+, K+ and Cl-, while DH observed a significant increase. 3) HCO₃made a significant decrease at DH before growing up. 4) Mg++ had no change, while Ca++ increased significantly at RH and DH. 5) Lactate showed an increase with significance at RH and DH, especially greater at DH. 6) Osmotic pressure increased meaningfully at DH. 7) Anion gap increased significantly at DH and RH. 8) On the other hand, when the forced RH was held, the group of athletes had greater sweat loss per body surface area than that of non-athletes. 9) The athletes' plasma concentration of Na+ and Cl- revealed a clear decrease. 10) There was seen no significant change in the plasma concentration of K+, Mg++ and Ca++ in both groups. 11) The IVDP occurred at the RH of 2.3ℓfor athletes and more than 2.5ℓfor no-athletes.

Effect of pH, Light, Plant growth regulators and Concentration of inorganic salt on in vitro Masspropagation of Camellia sinensis (L.) O. Kuntze

Kil Ja Kim,Jung Woon Kim,Chang Yong Yoon,Jae Hong Kim,Tae Ho Han,Chang Hyu Bae 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.38 No.1

Fruiting Body Characteristic of Auricularia polytricha (Mont.) Sacc. “Geoni” by Differences of Cultivation Environment

Kil-Ja Kim,Dami Kim,Ho-Sub An,Myeong-Seok Kim,Dong-Kwan Kim,Heung-Gyu Park 한국버섯학회 2017 버섯 Vol.21 No.2

Auricularia polytricha has strong decomposition ability on wood fiber and a wide range of temperature adaptation as mid-high temperature mushroom. In addition, Auricularia polytricha does not require light in the mycelial growth period, but it is cultivated in the field because the light promotes the differentiation of fruiting bodies and affects the pigment formation. This study was conducted to investigate the possibility of the cultivation of Auricularia polytricha (Mont.) Sacc. "Geoni" which was bred at Jeonnam Agricultural Research Institute in 2012 at a simple-typed PE film mushroom house, and to compare the quality characteristics of the Auricularia polytricha (Mont.) Sacc. "Geoni" cultivated at air-conditioned mushroom house. Auricularia polytricha (Mont.) Sacc. "Geoni" was cultivated with sawdust-based media (0.9 kg) every 3 days from June 15 to July 13 at air-conditioned mushroom house and PE film mushroom house, and harvested until July 26th. The temperature and the humidity of the air-conditioned mushroom house was maintained at 23°C and 90-9 %. The Irrigation was conducted 1 time per day and 2 to 3 times per day at air-conditioned mushroom house and PE film mushroom house respectively. The harvesting period were the same for each treatment. The fruiting body was 6.0 ㎝ and 5.1 ㎝ in major axis and 4.6 ㎝ and 4.0 ㎝ in minor axis at PE film mushroom house and air-conditioned mushroom house respectively, as a result the size of the fruiting body grown at PE film mushroom house tended to be larger. The thickness of fruiting body was 0.85㎜ and 1.24㎜ at PE film mushroom house and air-conditioned mushroom house respectively. These results are considered to be due to the difference in the humidity control conditions between each treatment. The number of fruiting bodies per bag was 4.3 at PE film mushroom house and more tendency than 3.7 at air-conditioned mushroom house. The springiness of the fruiting body was 1.23 ± 0.21 and 1.01 ± 0.17. the chewiness was 620 ± 89 and 507 ± 128, and the resilience was 0.49 ± 0.03 and 0.35 ± 0.07 at PE film mushroom house and air-conditioned mushroom house respectively. As a whole, it was judged that the physical properties of the fruiting body grown at PE film mushroom house were excellent. Also, when comparing the chromaticity of fruiting body, L(lightness)-a(red color)-b(yellow color) value was 30-5.5-3.9 and 39-6.5-7.5 at PE film mushroom house and air-conditioned mushroom house respectively. The yields did not show differentiated tendency by cultivation time. However, the weight of fruiting body per bag was 308g and 252g at PE film mushroom house and air-conditioned mushroom house respectively. Therefore, according to these result, Auricularia polytricha (Mont.) Sacc. "Geoni" was judged to be a suitable cultivar at PE film mushroom house for summer.

식품중 치자황색소의 분석법에 관한 연구

김희연,김소희,홍기형,김미순,김현종,이주연,이영자,하상철,이철원,김길생 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-