느타리버섯 신품종 육성 연구(I) : 병재배용 신품종『진미』느타리버섯의 특성

지정현,주영철,최종인 한국버섯학회 2004 한국버섯학회지 Vol.2 No.4

병재배 느타리버섯의 품종 다양화를 위해 단핵균주교잡에 의해 육성된 진미느타리버섯의 균사생장적온은 25∼30℃, 버섯발생 및 생육온도는 15±1℃이고 갓색은 회색이며 얕은깔대기형으로 발이수가 많았다. 배양일수는 20℃에서 22일, 초발이 소요일수는 4일이며 가늘고 긴형으로 농가실증시험에서 병당수량은 145.9g으로 높은 편이었다. 버섯의 갓색과 갓형태, 이형개체 발생,, 균사생장량, 종균배양기간 등에 대한 균일성도 양호하였고, 세균성 갈변병에 대한 저항성은 소정도로 나타났으나 푸른곰팡이병은 발생되지 않았다. 또한 균사 활력이 강하고 15 이하에서는 진한회색을 나타내며 육질이 부드럽고 대가 백색으로 깨끗한 직립형의 특성을 보유하고 있다. Jinmi oyster mushroom(Pleurotus ostreatus) were bred in Mushroom Research Institute, Gyonggi Province A.R.E.S in 2003. This oyster mushroom were bred and cultivated one after mating single spores collected from K3-2 and ASI2018-249. The major characteristics of the mushroom are showing a lot of pinheadings, the gray-colored and infundibuliform pileus. The optimum temperature for the mycelial growth was around 25∼30℃ and that for the pinheading and growth of fruitbody was around 15±1℃. Around 22 days at 20℃ were required for incubation of Jinmi and the yield was shown high by 145.9g/bottle.

치주병원균에 대한 유산균의 억제효과

정하나,김영준,정현주,오종석 전남대학교 치과대학 1999 전남치대논문집 Vol.11 No.1

This study was performed to evaluate the effect of hydrogen peroxide-producing Lactobacillus acidophilus V-20 on the replication of periodontal pathogens, Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. When A. actinomycetemcomitans and P. gingivalis were incubated alone and in the combination with L. acidophilus V-20, the viable cell numbers of A. actinomycetemcomitans and P.gingivalis were compared between those cultures. The effect of S. mutans, E. durans, and L. lactis on the replication of A. actinomycetemcomitans and P. gingivalis was also evaluated. The change of periodontal indexes(probine depth, gingival index, GCF volume) and the viable cell numbers of A. actinomycetemcomitans and black pigmented bacteroides in subgingival plaque sample were evaluated following gargling of fermented milk made from L.acidophilus V-20 for 1 month on patients with periodontal disease in maintenance phase. In the mixed culture of L. acidophilus V-20 and A. actinomycetemcomitans or P. gingivalis, the replication of A. actinomycetemcomitans or P. gingivalis was completely inhibited. But in the mixed culture of P. gingivalis and hydrogen peroxide-nonproducing Lactobacillus casei, the viable cell numbers of P. gingivalis was not decreased when compared with the numbers in the mixed culture of P. gingivalis and L. acidophilus V-20. In the mixed culture of A. actinomycetemcomitans and S. mutans, E. durans, or L. lactis, the viable cell number of A. actinomycetemcomitans was not almost changed when compared with the numbers in the culture of A. actinomycetemcomitans alone. And in the mixed culture of P. gingivalis and E. durans or L. lactis, the viable cell numbers of P. gingivalis was not almost changed compared with the counts in the culture of P. gingivalis alone. But the replication of P. gingivalis was completely inhibited in the mixed culture of P. gingivalis and S. mutans. When the change of periodontal indexes following gargling of fermented milk was compared with baseline, probing depth and gingival index were not changed, but GCF volume was significantly decreased(p<0.05). And when the viable cell numbers of microorganisms in subgingival plaque sample were compared with baseline, total viable cell number was almost unchanged and the viable cell numbers of A. actinomycetemcomitans and black pigmented bacteroides were significantly decreased(p<0.05). These results suggest that L. acidophilus V-20 inhibit the replication of A. actinomycetemcomitans and black pigmented bacteroides by the formation of hydrogen peroxide.

척수를 절단한 흰쥐에 태생기의 Serotonin을 함유한 솔기핵을 이식한 후 척수의 형태학적 재생 및 기능에 관한 연구

김종중,정윤영,장인엽,정주현,문정석,김흥중 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.6

유기농 자재의 시용이 토양의 이화학적 성질과 상추, 가지의 생육 및 체내성분에 미치는 영향

이종성,김기영,황필성,오주성,정원복,정순재 동아대학교 2001 大學院論文集 Vol.26 No.-

This study was carried out to investigate the effect of microbial fertilizers on the application of organic farming materials in soil on growth and yields of leaf lettuce, eggplant. Three microbial fertilizers, Aika, Kalen-gold and EMRO-EM were used. The results were summarized as follows ; 1. The effect of soil chemical properties after application organic arming materials, The amounts of pH, EC, P2O5 and O.M. treated all plot were few of change. 2. Change of soil microbial after application organic farming materials, The number of bacteria, actinomycetes and fungi in N, P, K treated plot showed the increased tendency, to the contrary, The number of bacteria, actimycetes in Aika, kalen-gold and EMRO-EM treated plot showed the increased, The number of fungi showed the dicreased tendency 3. The application effects of Aika, Kalen-gold and EMRO-EM on the growth, yield and components of leaf lettuce and eggplant in comparison with chemical fertilizers plot was not a difference.

水盜作에서 親環境農業 定着을 위한 오리 및 왕우렁이 農法의 可能性 分析

이종성,김기영,황필성,오주성,정원복,정순재 동아대학교 농업생명과학연구소 2001 農業生命資援硏究 Vol.10 No.1

Concerns on use of excess amount of chemical fertilizer and pesticide in current farming system turns both of the producer and consumer of agricultural products to an organic farming which use a less chemicals and more natural manure. Possibility Analysis of Pond-snail and Duck Faming for Environment-friendly Agriculture in paddy field. Depression effect of weed occurrence by pasture of 5kg of the Pond-snail(Ampullarius insularus) per 10a and the 7th day after transplanting. Optimum duck rasing density in rice paddy field was was 25~30 heads per 10a and ducks could be harvested three times in a year starting from 40 days after mature-seedling machine transplanting. The weed control rate of the duck raised paddy field was superior to the conventional agriculture. Yield per 10a of the duck raised and pond-snail(Ampullarius insularus) farming was inferior to conventional agriculture. The earnings of The Duck and Pond-snail farming realize a profit. From these results, it seems that the Organic farming are usefel and pasture of the Duck, Pond-snail(Ampullarius insularus) for depression of the weed occurrence is effective as the environmental perservation farming.

農民所得增大를 위한 고구마의 栽培와 利用에 관한 硏究

黃鍾奎,梁熙天,崔定植,權涌周 全北大學校 1977 論文集 Vol.19 No.-

New varieties of sweet potato Sinmi and Hwangmi were cultivated in the reclaimed hilly area of four kinds of soil series in Jeonbug province and their growth conditions such as cutting time, row spacing, application of fertilizers and plant growth regulators and covering effect were investigated. Besides these studies on cultivation of sweet potatoes, experiments on the change of chemical components of sweet potato tubers, adaptibility for storage and drying of sliced sweet potato tubers were carried out. The results are summarized as follows : 1. The early cutting of May 20, alley of 90 X 20cm, application of potassium(28kg/10a)and covering produced the high yield of sweet potatoes. The apllication of OED green did not increase the yield of sweet potatoes, 2. The yield of Hwangmi was higher than that of Sinmi. These two varieties of sweet potatoes grew well in all the test soil series except Bansan. By early cutting of May 20, the highest yield of Hwangmi was obtained in Songjung series soil and the highest yield of Sinmi was in Yesan. 3. Starch content was higher in Sinmi than in Hwangmi while yield of starch per unit area was high in Hwangmi because of its high yield of sweet potato tubers. Both Hwangmi and Sinmi showed very high water content of more than 70 per cent. The highest yield of total solid matter from Sinmi was on September 15 and that from Hwangmi on September 30. Sinmi and Hwangmi harvested on September 30 showed the highest starch content. 4. Variation in water content, starch, soluble sugar, protein and fat content during storage was minimum in the sweet potatoes stored in the temperature controlled storage chamber and was maximum in those stored in the cave under ground. As a result, percent of grade A sweet potatoes remaining was highest in the temperature controlled storage chamber and lowest in the cave under ground. 5. Hwangmi was more susceptible to Rhizopus nigricans than Sinmmi, especially under the humid condition. 6. Shape and thickness of slice did not affect the yield of dried sweet potatoes but affected the time needed for drying. Depending on the thickness and the shape of slice, variations of 36 hours and 12 hours in drying time were observed respectively. Drying table was most effective in drying of sweet potatoes and drying rack was also effective and required 2 days less time than mat. Dipping in water did not show any effect on drying time but improved the color of dried sweet potatoes.

중합효소연쇄반응을 이용한 도말양성 폐결핵 환자에서의 결핵균 DNA 검출방법에 관한 연구

이종진,김애경,조해정,한표성,홍석철,김주옥,김선영 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

Polymerase chain reaction (PCR) is the most sensitive and rapid method for the diagnosis of M.tuberculosis infection. Many reports have shown different methods for mycobacterial DNA extraction, but revealed many differences in simplicity and time-consumption. We studied which method was the best among 7 different ones for DNA extraction from 32 smear-positive sputa, using PCR of targeting 123bp DNA fragment of IS6110. The methods were the following : SDS-Microwave oven method, NaOH Lysis method, Triton X-100, Proteinase K method, Lysis buffer method. SDS-Proteinase K method, Bead Beater method, and Bead Beater/CTAB method. The amplification products were identified by electrophoresis on ethidium bromide-stained agarose gels, and confirmed by restriction enzyme, Southern blot hybridization, and DNA sequencing. All 32 cases were positive (100%) by the 7 different methods. It took 1 hour or more to detect DNA in SDS-Microwave oven method, NaOH Lysis method, and Bead beater/CTAB method. It took 2 hours or less in the others. SDS-Proteinase K method and Bead Beater/CTAB method were simpler and more convinient than the others. These results suggest that SDS-Proteinase K method and Bead Beater/CTAB method might be better than the others for the extraction of mycobacterial DNA in terms of simplicity and time-consumption, although all the methods were sensitive.

토끼의 간뇌에서 NPY 분비세포에 대한 면역조직화학적 연구

정주현,문정석,김종중,장인엽,정윤영,김흥중 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

The distributions of neuropeptide Y(NPY) in the diencephalon of the adult rabbit was investigated using indirect immunohistochemistry. The method showed that neuropeptide Y had a widespread distributions and was present in extremely high concentration in the hypothalamic nuclei of the rabbit. A few number of NPY-containing perikarya was found in the arcuate nucleus, paraventricular nucleus. medial to the fornix. Neuropeptide Y-immunoreactive axons and terminals in the hypothalamus were found in most hypothalamic nuclei. The highest concentrations of neuropeptide Y-containing fibers were found in the arcuate nucleus. paraventricular nucleus. and alao the supraoptic nucleus. dorsomedial hypothalamic nucleus. preoptic area showed relatively high concentration of neuropeptide Y-immunoreactive fibers. The extremely high concentations of neuropeptide Y in the bypothalamus indicate important role in many brain functions

법랑모세포 분화와 법랑질 형성과정에서 OD314, Apin protein의 발현 및 기능

박종태,최용석,김흥중,정문진,오현주,신인철,박주철,손호현 대한치과보존학회 2006 Restorative Dentistry & Endodontics Vol.31 No.6

본 연구에서는 법랑모세포 분화와 법랑질 형성에 연관이 있는 OD314 일명 Apin protein의 기능을 밝힐 목적으로, in-situ hybridization에 의한 OD314 mRNA 발현과 법랑모세포 세포주에서 OD314 enamel matrix protein의 발현, 그리고 OD314 유전자를 과발현/억제시킬 수 있는 construct를 제작한 후 법랑질 형성 중에 OD314의 기능을 알아보고자 RT-PCR를 시행하여 다음과 같은 결과를 얻었다. 1. OD314 mRNA는 발생중인 상아모세포보다 법랑모세포에서 강하게 발현되었다. 2. Tuftelin은 석회화 결정이 형성되는 14일까지 발현이 지속되고, 그 이후부터 점차 감소하였다. Amelogenin과enamelin은 7일부터 그 발현이 점점 감소하였다. 3. U6-OD314 siRNA construct를 이용하여 transfection한 법랑모세포 세포주는 OD314와 tuftelin,MMP2 mRNA 발현이 감소하였으며, CM-OD314를 transfection하여 OD314의 과발현을 유도한 경우에는 OD314와 MMP20 mRNA의 발현이 뚜렷이 증대되었다. 이 결과는 OD314가 법랑모세포의 분화와 법랑질의 형성 그리고 석회화 과정에 중요한 역할을 하는 새로운 인자임을 시사한다. This study was aimed to elucidate the biological function of OD314 (Apin protein), which is related to ameloblast differentiation and amelogenesis. Apin protein, calcifying epithelial odontogenic (pindborg) tumors (CEOTs)-associated amyloid, were isolated from CEOTs, and has similar nucleotide sequences to OD314. We examined expression of the OD314 mRNA using in-situ hybridization during tooth development in mice. Expression of OD314 and several enamel matrix proteins were examined in the cultured ameloblast cell line up to 28 days by reverse transcription-polymerase chain reaction (RT-PCR) amplification. After inactivation and over-expression of the OD314 gene in ameloblast cell lines using U6 vector-driven RNA interference and CMV-OD314 construct, RT-PCR were performed to evaluate the effect of the OD314 during amelogenesis. The results were as follows: 1. In in-situ hybridization, OD314 mRNAs were more strongly expressed in ameloblast than odontoblast. 2. When ameloblast cells were cultured in the differentiation and mineralization medium for 28 days, the tuftelin mRNA expression was maintained from the beginning to day 14, and then gradually decreased to day 28. The expressions of amelogenin and enamelin were gradually decreased according to the ameloblast differentiation. 3. Inactivation of OD314 by U6-OD314 siRNA construct down-regulated the expression of OD314, MMP-20, and tuftelin, whereas over-expression of OD314 by CMV-OD314 construct up-regulated the expression of OD314 and MMP-20 without change in tuftelin. These results suggest that OD314 is considered as an ameloblast-enriched gene and may play the important roles in ameloblast differentiation and mineralization.

군산 연안 해역에서의 부영양화 제어에 관한 연구

김종구,정태주 한국환경과학회 2003 한국환경과학회지 Vol.12 No.9

Gunsan coastal area is one of region increasing pollution problems. To improve water quality, the reduction of these nutrients loads should be indispensible. In this study, the three-dimensional numerical hydrodynamic and ecosystem model were applied to analyze the processes affecting the eutrophication. In field survey, the average concentrations of dissolved inorganic nitrogen (DIN) and dissolved inorganic phosphorus(DIP) at surface waters were found to be 0.43㎎/ℓ and 0.03㎎/ℓ respectively, which were exceeding second grade of water quality criteria. In hydrodynamic modelling, the comparison between the simulated and observed tidal ellipses showed fairly good agreement. The ecosystem model was calibrated with the observed data in study area. The simulated results of DIN were fairly good coincided with the observed values within relative error of 32.39%, correlation coefficient(r) of 0.99. In the case of DIP, the simulated results were fairly good coincided with the observed values within relative error of 24.26%, correlation coefficient(r) of 0.82. The simulations of DIN and DIP concentrations using ecosystem model were performed under the conditions of 20~80% reductions for pollutant loading. At simulation results, concentration of DIN and DIP were reduced to 20~80% and under 10% in case of the 80% reduction of polltuant loading, respectively.