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최홍준,이해혁,김태희,김정식,남계현,이권해,박성진 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2
Arteriovenous fistula of uterus is a very rare cause of massive uterine bleeding. However, it is important to make a rapid and precise diagnosis when it does occur, because life-threatening massive vaginal bleeding may occasionally be a symptom of this disease. Uterine curettage or surgical trauma can cause vascular abnormalities, including pseudoaneurysms, acquired ateriovenous malformations, ateriovenous fistulas, and rupture of vessels. The diagnosis is made by angiography, and gray scale ultrasonography, color and duplex doppler ultrasonography, computed tomography, magnetic resonance imaging is helpful. Ultrasonography is the most commonly performed initial imaging examination for evaluation of abnormal uterine bleeding. Color and duplex Doppler US is an appropriate modality for the detection and diagnosis of uterine ateriovenous malformations and for follow-up after embolization. Transcatheter embolization has replaced hysterectomy as the treatment of choice in women wish to retain thier fertility. A case of arteriovenous malformation of the uterus which was diagnosed by ultrasonography. So reported this case with a brief review of the literature.
결정형 규산분진에 폭로된 섬유모세포의 자가증식 : Evaluation by H₂O₂and PDGF-AA 와 TGFβ
안병용,김경아,문제혁,정진숙,김은경,임영 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2
Objectives : The aim of this study is to find out the activity of autoproliferation of rat fibroblast exposed to crystalline silica and the role of mediators secreted from rat fibroblast. Methods : The effect of α-quartz on production of growth factor (platelet-derived growth factor-AA and transforming growth factorβ) from rat fibroblasts were evaluated by ELISA and immunocytochemical analysis. Gene expression of these growth factors in rat fibrobast exposed to crystalline silica was evaluated by RT-PCR. Furthermore, fibroblast proliferation by culture supernatant of rat fibroblast was assayed by the neutral red test. Results : The amounts of H2O2 and growth factors synthesized in rat fibroblasts were significantly increased by the stimulation of crystalline silica (α-quartz) , which showed the dose-dependent manner to the concentration of α-quartz with the maximum response at the dosage of 100 ㎍/㎠. The result of RT-PCR demonstrated that α-quartz induced gene expression of PDGF-AA and TGFβ in rat fibroblast. We also found that supernatant of α-quartz-cocultured rat fibroblast induced a significant proliferation of fibroblast. Conclusion : Crystalline silica directly induce functional change In fibroblast such as increased release of reactive oxygen species and growth factors. The products of these functional change promote fibroblast proliferation via autocrine loop.
김경아,남혜윤,문제혁,정진숙,임영,정치경 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.4
목적 : Rat2 섬유모세포에서 α-quartz에 의해 유도된 NOS의 조절기전을 알아보기 위하여 본 연구가시도 되었다. 방법 : Rat2 세포에 있어서 α-quartz 자극에 의한 NO와 H₂O₂의 분비 및 iNOS의 발현을 관찰하였고, iNOS 억제제인 L-NIL과 H₂O₂ 억제제인Catalase를 전처리하고 마찬가지로 NO와 H₂O₂분비 그리고 iNOS의 단백발현 정도를 western blot-ting을 이용하여 관찰하였다. 결과 : o-quartz는 Rat2 세포에서 iNOS에 의한NO와 H₂O₂의 분비를 유도하였다. L-NIL은 R3t2세포에서 D-quartz에 의한 H₂O₂의 분비와 iNOS발현을 완전히 억제하였다. Catalase로 전처리 하였을 때 o-quartz로 유도된 iNOS에 의하여 생산증가된 H2Oa가 iNOS를 억제하는 autoinhibitorypathway가 차단되어 결과적으로 H₂O₂의 분비와 iNOS의 발현이 증가하였다. 결론 : Rat2 세포에서 α-quartz에 의하여 유도된iNOS는 H₂O₂의 분비를 촉진하고 이 H₂O₂는 iNOS기능을 조절하는 자가조절기전에 중요한 역할을 하는 것으로 생각된다. Objectives : This study was performed in order to investigate the molecular mechanism regulating nitric oxide synthase (NOS) induced by α-quartz in Rat2 fibroblast. Methods : α-quartz-induced nitric Oxide (NO) anti H₂O₂ formation anti α-quarts-induced iNOS protein expression in Rat2 fibroblast were monitored. With iNOS inhibitor (L-N6- (1-iminoethyl) lysine hydrochloride, L-NIL) or antioxidant (catalase), we observed NO and H₂O₂ formation and iNOS protein expression in Rat2 fibroblast stimulated with α-quartz. Results : α-quartz stimulated iNOS-induced NO and H₂O₂ formation in Rat2 fibroblast. L-NIL inhibited H₂O₂ formation and iNOS protein expression by α-quartz in Rat2 fibroblast. Pretreatment with catalase blocked the autoinhibitory pathway of iNOS by iNOS-induced H₂O₂ therefore H₂O₂ and NO production and iNOS protein expression were increased in Rat2 fibrobalst stimulated with α-quartz Conclusion : α-quartz-induced iNOS Stimulated H₂O₂ formation in Rat2 fibroblast. INOS-induced H₂O₂ by α-quartz plays an important role in the autoinhibition pathway for regulating the iNOS function in Rat2 fibroblast
Feeding by the Pfiesteria-Like Heterotrophic Dinoflagellate Luciella masanensis
JEONG, HAE JIN,HA, JEONG HYUN,YOO, YEONG DU,PARK, JAE YEON,KIM, JONG HYEOK,KANG, NAM SEON,KIM, TAE HOON,KIM, HYUNG SEOP,YIH, WON HO Wiley (Blackwell Publishing) 2007 JOURNAL OF EUKARYOTIC MICROBIOLOGY - Vol.54 No.3
<P>To explore the feeding ecology of the Pfiesteria-like dinoflagellate (PLD) Luciella masanensis (GenBank Accession no. AM050344, previously Lucy), we investigated the feeding behavior and the kinds of prey species that L. masanensis fed on and determined its growth and ingestion rates of L. masanensis when it fed on the dinoflagellate Amphidinium carterae and an unidentified cryptophyte species (equivalent spherical diam., ESD=5.6 microm), which were the dominant phototrophic species when L. masanensis and similar small heterotrophic dinoflagellates were abundant in Masan Bay, Korea in 2005. Additionally, these parameters were also measured for L. masanensis fed on blood cells of the perch Lateolabrax japonicus and the raphidophyte Heterosigma akashiwo in the laboratory. Luciella masanensis fed on prey cells by using a peduncle after anchoring the prey with tow filament, and was able to feed on diverse prey such as cryptophytes, raphidophytes, diatoms, mixotrophic dinoflagellates, and the blood cells of fish and humans. Among the prey species tested in the present study, perch blood cells were observed to be the optimal prey for L. masanensis. Specific growth rates of L. masanensis feeding on perch blood cells, A. carterae, H. akashiwo, and the cryptophyte, either increased continuously or became saturated with increasing the mean prey concentration. The maximum specific growth rate of L. masanensis feeding on perch blood cells (1.46/day) was much greater than that of A. carterae (0.59/day), the cryptophyte (0.24/day), or H. akashiwo (0.20/day). The maximum ingestion rate of L. masanensis on perch blood cells (2.6 ng C/grazer/day) was also much higher than that of A. carterae (0.32 ng C/grazer/day), the cryptophyte (0.44 ng C/grazer/day), or H. akashiwo (0.16 ng C/grazer/day). The kinds of prey species which L. masanensis is able to feed on were the same as those of Pfiesteria piscicida, but very different from those of another PLD Stoeckeria algicida. However, the maximum growth and ingestion rates of L. masanensis on perch blood cells, A. carterae, H. akashiwo, and the cryptophyte were considerably lower than those of P. piscicida. Therefore, these three dinoflagellates may occupy different ecological niches in marine planktonic communities, even though they have a similar size and shape and the same feeding mechanisms.</P>
In Vitro Genotoxicity Assessment of a Novel Resveratrol Analogue, HS-1793
Jeong, Min Ho,Yang, Kwangmo,Lee, Chang Geun,Jeong, Dong Hyeok,Park, You Soo,Choi, Yoo Jin,Kim, Joong Sun,Oh, Su Jung,Jeong, Soo Kyung,Jo, Wol Soon Korean Society of ToxicologyKorea Environmental Mu 2014 Toxicological Research Vol.30 No.3
Resveratrol has received considerable attention as a polyphenol with various biological effects such as anti-inflammatory, anti-oxidant, anti-mutagenic, anti-carcinogenic, and cardioprotective properties. As part of the overall safety assessment of HS-1793, a novel resveratrol analogue free from the restriction of metabolic instability and the high dose requirement of resveratrol, we assessed genotoxicity in three in vitro assays: a bacterial mutation assay, a comet assay, and a chromosomal aberration assay. In the bacterial reverse mutation assay, HS-1793 did not increase revertant colony numbers in S. typhimurium strains (TA98, TA100, TA1535 and TA1537) or an E. coli strain (WP2 uvrA) regardless of metabolic activation. HS-1793 showed no evidence of genotoxic activity such as DNA damage on L5178Y $Tk^{+/-}$ mouse lymphoma cells with or without the S9 mix in the in vitro comet assay. No statistically significant differences in the incidence of chromosomal aberrations following HS-1793 treatment was observed on Chinese hamster lung cells exposed with or without the S9 mix. These results provide additional evidence that HS-1793 is non-genotoxic at the dose tested in three standard tests and further supports the generally recognized as safe determination of HS-1793 during early drug development.
Inhibitory Effects of Chicken Egg Yolk Antibody on Infection of Escherichia coli in Macrophage
Jin-Ju Lee,Dong-Hyeok Kim,Jeong-Ju Lim,Dae-Geun Kim,Gon-Sup Kim,Won-Gi Min,Hu-Jang Lee,Man-Hee Rhee,Hong-Hee Chang,Suk Kim 경상대학교 농업생명과학연구원 2012 농업생명과학연구 Vol.46 No.2
The present study evaluated the potential use of immunoglobulin prepared from egg yolk of chickens immunized with Escherichia coli K88 (IgY-Ec) in the control of E. coli K88 infection in RAW 264.7 murine macrophage. The binding activity of IgY-Ec against E. coli K88 surface protein was more specific and increased than control IgY. In infection assay of E. coli in macrophage, the specific IgY-Ec to E. coli K88 remarkably inhibited the phagocytic activity comparing to nonspecific IgY (p<0.001). In adherence assay, bacterial adhesion on macrophage cells was definitely reduced by preincubation of IgY-Ec compared with nonspecific IgY (p<0.05). These findings suggested that IgY-Ec have the protective effects against pathogens and IgY-based diets may have potential benefits for preventing or treating various infections in domestic animals.