Modulation of Gene Expressions and Enzyme Activities of Methionine Sulfoxide Reductases by Cold, Aba or High Salt Treatments in Arabidopsis

Oh, Jee-Eun,Hong, Suk-Whan,Lee, Yunsook,Koh, Eun-Ji,Kim, Kideok,Seo, Youngwon,Chung, Namhyun,Jeong, Moonsoo,Chang,Chang, Cheolseong,Lee, Byoungcheon,Kim, Kyoung Heon,Lee, Kwangwon,Lee, Hojoung 생명자원연구 2005 생명자원연구 Vol.13 No.-

실시간 분광 엘립소메트터를 이용한 a-Si박막의 crystallization현상 연구

오영록,최은호,이기용,방경윤,안일신,김옥경 한양대학교 이학기술연구소 2004 이학기술연구지 Vol.7 No.

실시간 측정과 in-situ 측정을 하기 위해 실시간 분광 엘립소메트터 (real-time spectroscopic rotating compensator ellipsometer) 를 제작 방법을 연구하였다. 높은 온도 에서 얻어지는 Poly-Silicon은Glass에 변형이 생기므로 저온에서의 Poly-Silicon을 만들기 위하여 ni기판에 a-Si 박막을 성장 시킨후 그위에 투명전극을 올려 300℃ 이하의 저온에서 Ni과 투명전극에 전기장을 걸어 주었을때 a-Si이 Poly-Silicon 으로 바뀌는 crystallization 현상을 직접 제작한 ellipsometer 를 이용하여 실시간으로 관찰하였다. We make real-time spectroscopic rotating compensator ellipsometer for real time measurement and in-situ measurement. We study a method to make Poly silicon after depositing amomhous silicon thin film by dc sputter system at different temperature and pressure. Amorphous silicon films of 30㎚ thickness were deposited on the Ni substrate, and then ITO films of 20㎚ thickness were deposited on the a-Si films. The sampie were crystallized below 300℃ in the electric field applied between the Ni and ITO.

Chlorhexidine 처리가 상아질 접착제의 미세인장결합강도에 미치는 영향

오은화,최경규,김종률,박상진 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.2

본 연구는 상아질 혼성층의 교원섬유를 가수분해하는 효소인 MMPs (Matrix metalloproteinses) 억제제로 알려진 chlorhexidine (CHX)을 적용 후 결합강도를 측정하였으며, 이를 각각 열순환 처리 후 결합강도를 측정하였다. 또한 주사전자현미경으로 접착계면메서의 파괴 양상을 비교 분석하였다. 우식이 없는 발거한 32 개의 제 3 대구치의 교합면 상아질을 노출시키고 GI그룹에서는 dentin conditioner를 처리 후 2% chlorhexidine을 적용시키고, 산부식 접착제 그룹에서는 인산 산부식을 시행하고 2 % chlorhexidine을 적용 후 3단계 산부식형 상아질 접착제 (Scotchbond Multipurpose, SM), 2단계 산부식형 상아질 접착제 (Single Bond, SB)를 도포하고, 자가부식 접착제 그룹에서는 2 % chlorhexidine 적용 후 자가부식 상아질 접착제 (Clearfil Tri-S, TS)를 도포한다. 이후 복합 레진 (Z-250)과 GI (Fuji-II LC)를 충전한 시편을 1 ㎟의 단면을 갖는 beam으로 제작하여 열순환 하지 않거나, 10,000 회 열순환 (5 ~ 55 ℃)하였다. Universal testing machine (EZ-test: Shimadzu, Japan)에서 cross head speed 1 mm/min로 인장력을 가하여 , 미세인장결합강도를 측정하였다. 실험 결과는 유의수준 0.05 1evel에서 two-way ANOVA를 이용하여 통계분석하였다. 그 후 파절된 시편의 파괴 양상을 현미경 (SEM)으로 관찰하여 다음과 같은 갈론을 얻었다; 1. 2 % CHX을 적용한 모든 실험군에서 상아질과의 미세인장결합강도가 증가하였고, 열순환은 상아질과의 미세인장 결합강도를 감소시켰다 (P>0.05). 2. CHX 적용 후 열순환 한 군은 CHX을 적용하지 않고 열순환한 군에 비하여, 상아질과의 미세인장결합강도가 높았으며 , 특히 GI와 TS군에서 유의한 차이를 나타내었다 (P<0.05). 3. 파괴 양상 분석 결과, 혼성층에서의 접착성 파괴를 보이며, CHX을 적용하면 혼성층 기저부에서 상부로 파괴 부위가 옮겨가는 양상을 나타내었다. 이상의 연구 결과를 토대로, MMPs 억제제인 2 % CHX은 글래스 아이오노머 시멘트와 상아질 접착제의 초기 미세인장결합강도에는 영향을 미치지 않으며 ,CHX 적용이 접착 내구성을 유지하는데 도움이 되었다. The purpose of this study was to evaluate the effect of chlorhexidine (CHX) on microtensile bond strength (μTBS) of dentin bonding systems. Dentin collagenolytic and gelatinolytic activities can be suppressed by protease inhibitors. indicating that MMPs (Matrix metalloproteinases) inhibition could be beneficial in the preservation of hybrid layers. Chlorhexidine (CHX) is known as an inhibitor of MMPs activity in vitro. The experiment was proceeded as follows : At first, flat occlusal surfaces were prepared on mid-coronal dentin of extracted third molars. GI(Glass lonomer) group was treated with dentin conditioner, and then, applied with 2 % CHX. Both SM (Scotchbond Multipurpose) and SB (Single Bond) group were applied with CHX after acid-etched with 37% phosphoric acid. TS (Clearfil Tri-S) group was applied with CHX, and then, with adhesives. Hybrid composite Z-250 and resin-modified glass ionomer Fuji-II LC was built up on experimental dentin surfaces. Half of them were subjected to 10,000 thermocycle, while the others were tested immediately. With the resulting data, statistically two-way ANOVA was performed to assess the μTBS before and after thermocycling and the effect of CHX. All statistical tests were carried out at the 95 % level of confidence. The failure mode of the testing samples was observed under a scanning electron microscopy (SEM). Within limited results, the results of this study were as follows; 1. In all experimental groups applied with 2 % chlorhexidine, the microtensile bond strength increased, and thermocycling decreased the microtensile bond strength (P > 0.05). 2. Compared to the thermocycling groups without chlorhexidine, those with both thermocycling and chlorhexidine showed higher microtensile bond strength, and there was significant difference especially in GI and TS groups.

면접조사를 통한 초등학생의 영양교육 실태 및 실천도 조사

오유진,이영미,김정현,안홍석,김정원,박혜련,서정숙,김경원,권오란,박혜경,이은주,성현이 대한지역사회영양학회 2008 대한지역사회영양학회지 Vol.13 No.4

This study investigated the experience and practice of elementary school students on nutrition education. The data were collected from 217 male and female students attending 5-6th grade elementary schools in Seoul and Kyunggi-Do from March to June 2007, interviewing face to face by a nutrition teacher and 3 interns of a nutrition teacher. The results were as follows: 86.5% of the subjects learned about ‘Table etiquette’, ‘Reasons for eating fruits and vegetables’ (78.7%), ‘Food waste and environment’ (72.3%), ‘Healthy snacks’ (55.7%), ‘Food sanitation’ (52.3%), ‘Food culture of foreign countries’ (48.1%). Nutrition education experience was significantly different by gender. A total of 43.5% boys responded that they never learned about ‘basic food preperation’ (p < 0.01). They had learned ‘Nutrients for body’ and ‘Food waste and environment’ in school, ‘Healthy weight loss’, ‘Food culture of foreign countries’, ‘Food circulation’ on television, Most content (‘Table etiquette’, ‘Simple cooking’, ‘Food sanitati n’, ‘Eating behaviors for health’, ‘Reasons for eating fruits and vegetables’, ‘Healthy snacks’) was learned from parents. The practice after nutrition education was higher in ‘Table etiquette’ (2.14), ‘Eating fruits and vegetables’ (2.07) than others compared with education experience. The most reason of non-practice on nutrition information was ‘Troublesome’. In ‘Nutrients for body’, a boy answered ‘Difficult for practice’ 20.0%, a girl answered ‘Difficult to understand’ 32.6%, showing a significant difference between the gender groups (p < 0.001). They remembered the ‘Nutrients for body’ (49.6%), ‘Food sanitation’ (44.5%) because of ‘important content’,‘Basic food preparation’ (40.6%), ‘Food culture of foreign countries’ (36.3%) because of ‘interesting content’, ‘Healthy weight loss’ (52.0%), ‘Eating behavior for health’ (44.5%) and ‘Healthy snacks’ (33.7%) because of ‘need for my health’.

폐부종과 심정지가 발생한 페노바비탈 중독환자

오동렬,이운정,박규남,김세경,김영민,이환,유은영,최경호,정시경 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Phenobarbital is a long-acting barbiturate causing generalized depression of neuronal activity in the brain. Its effect is primarily achieved through enhanced GABA-mediated synaptic inhibition. Its use as an antiepileptic agent was first described in 1912. Before the introduction of phenytoin, phenobarbital is used as sedative-hypnotics. It is used for the treatment of epilepsy and status epilepticus. All barbiturates, including phenobarbital, have a high potential for abuse. They were frequently used for suicide attempts in the past, but they have in large part been replaced by benzodiazepines. The onset of symptoms depends on the drug and the route of administration. Mild to moderate barbiturate intoxication resembles ethanol inebriation with slurred speech, ataxia, and lethargy. Severe acute barbiturate intoxication is life threatening. Early deaths are generally cardiovascular-related. Hypotension, shock, pulmonary edema, and cardiac arrest that occurs with large doses are caused by depression of central sympathetic tone and as well as by direct depression of cardiac contractility. The potentially fatal oral dose of phenobarbital is 6-10g. We describe an 23-year-old woman with pulmonary edema, and cardiac arrest ingestion of 18grams of phenobarbital. She was completely recovered by successful cardiopulmonary resuscitation and homoperfusion. We report a case with literature review.

응급환자의 중증도 분류를 위한 지표의 개발 : Triage Score와 Modified Triage Score NEW METHODS TO TRIAGE ALL EMERGENCY PATIENTS

임경수,홍은석,김선만,황성오 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

Up to now, triage system for all emergent patients, which can be used fast and conviently according to their severity has not been developed yet. For the triage of trauma patient, emergency medical technicians and emergency medical doctors are commonly using R.T.S. (Revised Trauma Score). The R.T.S. consists of GCS(Glasgow coma scale), systolic blood, pressure and respiration rate per minute. But it is difficult for emergency medical technicians to calculate GCS in the prehospital because of its complexity and need for more time. Therefore we develop the Triage Score by remodelling the R.T.S. by replacing GCS with AVPU scale and systolic blood pressure that is under 49 mmHg after adding 1-49 mmHg with 0 mmHg and respiration rate per minute that is under 5/min after adding 1-5/min with 0/min. We stuied the significance and the possible triage application of Triage Score in traumatic emergency patients. For nontraumatic emergency patients, we develop the Modified Triage Score to triage the cardiovascular emergencies by adding the item of chest pain to Triage Score. A prospective study was carried out on 530 patients from March 1 to 31 1996. There were 210 trauma patients and 320 nontraumatic emergency patients and the result shows as follows. For trauma patients, there was linear correlation between R.T.S. and Triage Score(R²=0.965). Comparison analysis between high risk group (admission+death patients) and low risk group(discharge patients) of nontraumatic emergency patients by using Modified Triage Score is statistically significant(P<0,001) The cardiovascular emergencies could not categorized as urgency by Triage Score, but that was possible by application of Modified Triage Score. In conclusion, we can triage the injured patients effectively by Triage Score and the nontraumatic emergency patients including emergency cardiovascular disease can be categorized as urgency by applying the Modified Triage Score.

가성부갑상선 기능저하증 환아의 구강 증상 : CASE REPORT

김성오,홍은경,최형준,이제호,손흥규 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.2

부갑상선 호르몬은 혈중 인농도를 낮추고, 칼슘농도를 증가시키는 호르몬으로 부갑상선기능저하증은 부갑상선이 존재하지 않거나 비정상적으로 기능하여 이 호르몬의 분비가 감소되는 질환이며, 가성부갑상선기능저하증은 부갑상선 호르몬 분비는 정상이나, 이 호르몬에 대한 괄과 신장의 반응이상으로 혈중 칼슘 농도가 감소되고 인농도가 증가되는 질환이다. 가성부갑상선기능저하증은 임상적으로 성장 및 발육부전, 둥근 얼굴, 조기 골단폐쇄로 인한 단지증, 이소성 연조직 석회화, 비만, 두개관의 비후, 정신지체, 백내장 등이 나타난다. 구강내 소견으로는 치아의 맹출지연, 법랑질형성부전이 가장 큰 특징이며, 그 외에 결손지, 부정교합, 높은 구개궁, 확장된 치근관과 짧은 치근, 치수강 내 석회화, 높은 우식 이환율, 치근막의 비후 등이 관찰된다. 본 증례에서 환아의 구강내 소견은 맹출한 하악 4전치와 상악 좌우측 영구 중절치, 상악 우측 영구 측절치, 상, 하악 제 1대 구치에서 법랑질형성부전을 보였고, 다수의 유치에서 심한 동요도를 보였다. 방사선 소견으로는 대부분의 미맹출된 영구치들도 법랑질형성부전이 관찰되었으며, 특히 영구 견치의 경우 심한 치관의 형태이상을 볼 수 있었다. 또한 유치의 비정상적인 치근흡수가 나타났고, 하악 전치의 경우 치근형태가 짧고, 치근단이 둥근 형태로 나타났다. 이에 저자는 가성부갑상선기능저하증인 환아의 구강내 소견에 대해 보고하고자 한다. The parathyroid hormone plays a major role in the metabolism of calcium and phosphorus. In hypoparathyroidism the parathyroid glands are atrophied or absent associated with autoantibodies against parathyroid tissue. Pseudohypoparathyroidism is a metabolic disease caused by the disturbance in peripheral action of parathormone, but parathormone level is normal. In general, patients with pseudohypoparathyroidism have short stature, round face, brachydactylia, obesity, mental retardation, cataracts & ectopic calcifications on soft tissues. Dental manifestations are enamel hypoplasia, delayed eruption, blunting of root apex, hypodontia, pulp calcification, thickened lamina dura, excessive caries & malocclusion. In this case, intraoral examination showed enamel hypoplasia on the erupted permanent teeth & hypermobility on the remaining deciduous teeth. From the radiographic view, severe dental anomalies were observed on canines and shortening and blunting of root apex was observed on mandibular incisors. Pathologic root resorption was also observed on deciduous teeth.

非特異性 多發性 神經炎의 筋電圖所見

金敬姬,吳貞姬,李殷龍 大韓再活醫學會 1978 Annals of Rehabilitation Medicine Vol.2 No.1

Gas Chromatography를 이용한 Methoxime tert.-butyldimethylsilyl 유도체로서 α-Keto Acids의 분석에 관한 연구

金京禮,金正浩,金正漢,吳昌桓,黃恩卿 성균관대학교 약학연구소 1991 成均藥硏論文集 Vol.3 No.1

Abstract-Thirteen α-keto acids which are biochemically important were converted to methoxime(MO) derivatives with MOX and then to tert.-butyldimethylsilyl(TBDMS) derivatives using MTBS-TFA in isooctance prior to dual capillary column GC analysis. Single symmetric peaks were obtained for each MO TBDMS α-keto acids on the dual chromatograms. Optimum methoximation required heating at 60℃ for 2 hours and TBDMS derivatization required heating for 30 minutes. MO α-keto acids were stable during the tert.-butyldimethylsilylation and the methoximation of carbonyl group prevented silylation of enols. Mass spectrometric analysis of MO TBDMS derivatives was performed for structure identification. Base peaks at [M-57], intense ions at m/z=89, and weak ions at [M-15] were present in all spectra. The ions at m/z=89 were characteristic for α-keto acids.

남자대학생의 흡연태도와 행동에 관한 연구

강경진,김정미,민혜영,안소진,오경숙,은경희,이현,이희선,홍주미,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

There have been so many researches about problems of cigarettes and relation of smoking and heath since 1950's and generally known that smoking is one of the worst factor of increasing disease and mortality. No matter what such many proofs about various problems of smoking have been researched, smoking rate of current male collage students has not been decreased. Besides, most of the researches about cigarette use and its addiction have been focused on the adolescent or adult women smokers or adult male, not male collage student. Therefore, the subject of this study is about smoking attitude and smoking behavior of male collage students by general features. Smoking attitude was analyzed by modifying 'Guidelines for the content of tobacco smoking surveys of the general population'(WHO, 1982). Two hundred of male collage students participated to the distributed questionnaire for one week in May. The smoking attitude was categorized into duration of smoking and number of cigarette per day(CPD), and nicotine dependency, investigated by Fagerstrom Tolerance Questionnaire(FTQ) (Fagerstorm & Schneider, 1989). All statistics were performed by procedure of SAS according to number, percentage, mean, standard deviation, and ANOVA. The results are as follows: 1. Smoking attitude level was mean 25.73±5.15 marks, ranging from 13 to 39 marks of the total 40 marks. 2. Duration of cigarettes, number of cigarette per day, and nicotine dependency were mean 5.14±2.84 years, 12.88±6.85 pieces, and 4.78±1.69, respectively. 3. Smoking attitude showed significance according to general features such as marital state and allowance, but the significance according to marital state is not valuable because the numerical difference is so big between married one and single that they cannot be compared with each other. 4. Duration of smoking was significant according to general features such as age and allowance. 5. Smoking amount showed significance the factors of age only. 6. ll1ere was no significance in nicotine dependency according to general features. As a result, smoking attitude of male collage student in this study shows moderate level. Smoking attitude and duration is significant according to allowance, and smoking amount is significant according to age. Nicotine dependency according to general features is not significant but comparing with the preceding study about adult male, male collage students in this study are respectively more nicotine-dependent than adult male smokers. In conclusion, the right allowance usage strategies and effective heath education focused on the young are needed. In addition, helpful programs and information spreading strategies are necessary for establishing higher level of smoking attitude. At last, systemic regulations in daily living for ""anti-nico-dependent"" and perspective reforming of male university student are needed for reducing nicotine dependency.