Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

국내 혈액질환 환자에서 침습성 아스페르길루스증의 구제치료로서 Voriconazole의 효과와 안전성

권재철,김시현,최수미,최재기,이동건,박선희,최정현,유진홍,신완식 대한감염학회 2010 감염과 화학요법 Vol.42 No.1

Background: Invasive aspergillosis (IA) is associated with significant morbidity and mortality in patients with hematologic malignancies. We investigated the efficacy and safety of voriconazole (VCZ) when used as salvage therapy for IA in Korean adults with hematologic malignancies who had not responded to prior antifungal therapy. Materials and Methods: We retrospectively reviewed data, collected from January 2007 to October 2008, from patients with proven or probable cases of IA. All were probable IA cases, except for one proven case. All cases were refractory or intolerant to antifungal therapy prior to administration of VCZ. Efficacy and safety were assessed in patients treated with VCZ for more than 3 days and for more than one dose, respectively. A favorable response [complete (CR) or partial (PR)] was defined by significant improvement of all clinical symptoms, signs, and radiologic abnormalities. Results: Fifty patients who met the inclusion criteria were enrolled. There were 27 male and 23 female patients with mean age of 44.4 years (range, 15-65 years). Underlying diseases were acute leukemia (35 cases), chronic myelogenous leukemia (4 cases), myelodysplastic syndrome (3 cases), lymphoma (3 cases) and other hematologic diseases (5 cases). Twenty-two patients had received chemotherapy and 13 patients had undergone hematopoietic stem cell transplantation. The lung was the main infection site (94%) followed by the sinus (6%). Amphotericin B deoxycholate alone was the most frequent previous antifungal therapy. The mean duration of antifungal therapy prior to VCZ therapy was 13.9±8.8 days (2-44 days). The median duration of VCZ therapy was 19 days (interquartile range, 49 days). Sixteen patients (32.0%) showed favorable responses (CR:PR=8:8) at the end of VCZ therapy. The numbers of patients with stable disease, progression and death were, 6 (12%), 6 (12%) and 22 (44%) respectively. Most of those with unfavorable responses had relapsed underlying malignancies or refractory graft versus host diseases. Twelve patients developed drug-related adverse events but only one patient stopped VCZ treatment prematurely. Conclusions: VCZ demonstrated an acceptable level of toxicity in patients with hematologic malignancies but further studies are required to prove its efficacy as salvage therapy.

농촌지역 노인들의 우울증상에 관련된 요인들의 특성

송미숙,전기홍,송현종,김정은,박승구,신상건,이동훈,정성현,최연주,최완철,최민규,황재철,홍지만 아주대학교 1997 아주의학 Vol.2 No.2

This study was performed to identify risk factors for depression among the elderly in a rural area. A survey was done for the elderly living in Gosam-myenn, Ansung-kun during the period of July 28, 1997 and July 30, 1997. Demographic and sodoeconornic characteristics, and functional and health Status were assessed using a structured questionnaire, and the level of depression was measured by GDS score. The data obtained were analyzed by frequencies, percentage, t-test, ANOVA, and multiple regression, and the results were as follows: 1. The total sample consisted of 133 elderly. Of them, 47 elderly (35.4%) showed significant depressive Symptoms. 2. The GDS score was the highest for the group over 85 years of age (p < 0.05). Those living alone or widowed showed lower GDS score than those living with someone or married (p < 0.05). 3. The GDS score was the highest in the absolutely dependent group, followed by the partially dependent group, and the independent group (p < 0.01). The elderly who perceived their health Status as excellent showed the lowest GDS score. In contrast, those who had more than five chronic diseases showed the highest GDS score. 4. The elderly receiving financial and emotional support from their family or friends showed lower GDS score compared to those without any support. 5. The economically dependent group showed higher GDS score than that of independent group. 6. The elderly who involved in leisure activities showed lower GDS score than those without any involvement. 7. In terms of risk factors for depression, we found that perceived health Status, subjective economic Status, leisure activities, living arrangement and financial support were inversely associated with GDS score, whereas labor support was positively associated with GDS score. Based upon the above findings, this study suggests that these risk factors for depression in the elderly should be taken into consideration for a comprehensive mental health program for the elderly living in the rural area.

汎植物凝集素 Phaseolus vulgalis L.의 사람 血淸에 依한 凝集活性變化에 關한 硏究

崔完錫 大韓法醫學會 1990 대한법의학회지 Vol.14 No.1

All human red cells regardless of type were agglutinated by phosphate buffered saline extract of Korean Phaseolus vulgalis L. (P. vulgalis extract). By absorption of the extract with group AB human sera, certain red cells were apparently agglutinated while it was failed to agglutinate other red cells. Since such differences in reactivity were observed also among red cells of group O, it was apparent that the blood factor being detected by the absorbed P. vulgalis extracts, therefore, designated as ab. P(+) type(absorbed P. vulgalis positive type) of which the red cells gave marked agglutination with the absorbed P. vulgalis extracts and the red cells failed to agglutinate were ab. P(-) type(absorbed P. vulgalis negative type). In the experiment on a series of 589 specimens of red blood cells absorbed that the frequency of ab. P(+) type was 10.02%(59) and ab. P(-) type was 89.98%(530), and there were no differences in the distribution of ab. P type between male and female, among ABO blood groups, or healthy and sick persons.

초등학생들의 열과 온도에 대한 대안개념 조사

최행숙,김은경,백성혜,이길재,정완호 한국교원대학교 과학교육연구소 2001 청람과학교육연구논총 Vol.11 No.1

In this study, the contents of elementary school science textbooks related to 'heat and temperature' are analyzed and alternative conceptions of "heat and temperature" among students enrolled in grades 4, 5 and 6 are investigated. 259 students were selected from a elementary school located in urban area. The relationships between students' alternative conceptions and the content of elementary science textbooks are also examined. Students' conceptions are analyzed from their answers to a paper-and-pencil test. The typical alternative conception of "heat and temperature" held by students was follows. They think that "heat" is a material and "temperature" is value of heat amount. They can't distinguish between "heat" and "temperature". Their explanation of "heat and temperature" is focused on hot or cool sensation and other observable characteristics of a material. A textbook analysis indicated that contents on "heat and temperature" were organized without the viewpoint of particle motion theory. This may be one of the causes of students' alternative conceptions.

宋明 理學家詩의 特性에 관한 硏究

崔完植 韓國中國語文學會 1991 中國文學 Vol.19 No.-

魏晋·南北朝代의 自然詩에 關한 硏究

崔完植 서울대학교 교양과정부 1973 論文集 Vol.5 No.-

國際海峽의 上空飛行에 관한 法的考察

崔完植 한국항공대학교 1986 論文集 Vol.24 No.-

The freedom of overflight of international straits conferred by Article 38 of the Geneva Convention on the High Seas raises various questions as to the power of states bordering straits to restrict that freedom and impose obligations on aircraft in transit. Transiting aircraft mayconsiderably affect a littoral state's interests in regulating flight traffic, limiting dangerous or harmful activities such as flight exercises or refueling while in flight, and restricting military activities prejudicial to the defense or security of the coastal state. In examining some of the air law implications of the Law of the Sea (1982), two main issues arise. The first concernes the scope of the right fo transit passage for aircraft guaranteed by the Convention in international straits, archipelagic air routes, and exclusive economic zones. The term "transit passage" raises the question of possible restrictions on rights of overflight on the analogy of the question of "creeping jurisdiction." The Convention has introduced new areas of conflicting rights. International aviation, which is dependent on unambiguous operating rules, may have to face conflicting claims and legal requirements because of the uncertainties in the legal status of those new areas. This article deals with such new issues of international aviation law.

王守仁詩特性考

崔完植 韓國中國語文學會 1983 中國文學 Vol.10 No.-

植物凝集素 Dolichos labab L. 및 토끼赤血球에 의한 사람血淸型에 關한 硏究

崔完錫,文國鎭 고려대학교 의과대학 1985 고려대 의대 잡지 Vol.22 No.1

Since the introduction of phytohemagglutinin (PHA) by Stillmark in 1888, PHA contributed to medicolegal field with new classification of blood type by their agglutination activities to human red blood cells, and some of them have been used as lectins instead of antisera. But the majority of studies concerned to PHA confined to typespecific ones in classifying new types of red blood cells. For the purpose of new classification cf human serum, the author used Dolichos lablab L. extract, which act as panphytagglutin to rabbit red bloood cells, by use of inhibition property of human sera, serum stains, and whole blood stains to PHA and compared the results with various blood types. This study could be concluded a s follows. 1. There were two groups of human sera by their inhibition property on agglutination activity of Dolichos lablab L. extract to rabbit RBC. One had certain inhibiting substance and the other hadn't. 2. The frequency of former was 3%, and the latter was 97%. 3. Agglutination inhibition phenomenon of human sera on Dolichos lablab L. extract to rabbit RBC had to no relation with various blood and serum types, such as DL, DLI, ABO, Cl, H, ab.C. CCI, and haptoglobin. 4. This new classification of human sera is considered to be available for the classification of blood stain.