기계적 자극을 이용한 인체 중간엽줄기세포로부터 유사-섬유모세포로의 분화 유도

최경민 ( Kyung Min Choi ),서영권 ( Young Kwon Seo ),윤희훈 ( Hee Hoon Yoon ),권순용 ( Soon Yong Kwon ),이화성 ( Hwa Sung Lee ),박용순 ( Yong Soon Park ),손영숙 ( Young Sook Son ),송계용 ( Kye Yong Song ),김영진 ( Young Jin Kim 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.4

Recently, in vitro reconstruction of anterior cruciate ligament(ACL) has been tried using tissue engineering technique, especially mesenchymal stem cell(MSC) is considered as a good cell source. In this study, we tried to find the effect of mechanical stimulus on the differentiation of bone marrow-derived MSC into ACL fibroblastlike cell using a cell training bioreactor imposing cyclic mechanical tension whose parameters were 120 min/day, 1 Hz, and 5% strain. After 2 weeks of culture, MSCs under cyclic tension showed more regularly oriented alignment and lower expression of CD73 and CD105 than control by microscopy and FACS analysis. In transcriptional level, type I collagen, type II collagen, fibronectin, elastin, α-smooth muscle actin mRNA expressions of MSCs under cyclic tension were higher than control and similar to native ACL. In conclusion, it is thought that mechanical cyclic tension induced the loss of stemness of MSC, therefore improved the differentiation into ACL fibroblast-like cell.

성별에 따른 대학생의 내면화된 수치심과 대인관계 문제가 우울증상에 미치는 영향

최재정,김미연,박민지,유미숙 숙명여자대학교 아동연구소 2015 兒童硏究 Vol.27 No.1

본 연구는 대학생의 내면화된 수치심과 대인관계가 우울증상에 미치는 영향력을 규명하고자 한 연구이다. 연구 대상은 서울, 경기 지역의 대학생 404명을 대상으로 자기보고식 설문조사를 실시하였다. 본 연구에 사용된 측정도구는 대학생의 내면화된 수치심, 대인관계, 우울증상의 척도였다. 분석방법은 SPSS 19.0 통계프로그램을 이용하여 빈도와 백분율을 산출하였고, 내적합치도 계수(Cronbach a)를 구하였다. 본 연구문제를 여자 검증하기 위해 중다회귀분석과 단계적 회귀분석을 실시하였고, 밝혀진 결과를 요약하면 다음과 같다. 첫째, 여자 대학생은 사회적 회피와 헌신성이 우울증상과 관계가 있는 것으로 나타났다. 둘째, 남자 대학생은 실수 불안과 지배성, 비주장성이 우울증상에 영향을 미치는 것으로 나타났다. 셋째, 여학생과 남학생의 결과 차이에서 모두 우울 증상에 유의미한 영향을 미치는 것으로 나타났고, 수치심의 하위요인보다 대인관계문제의 하위요인들이 우울 증상에 보다 많은 영향을 미친다. 수치심보다 대인관계 문제가 우울 증상에 보다 큰 영향을 미친다는 결과는 남녀 대학생 모두에게 공통적으로 나타났다.

서울千戶洞地域調査

崔玲子,梁仁淑,禹敏子 梨花女子大學校 師範大學 社會生活科 1965 綠友硏究論集 Vol.- No.7

pefloxacin과 β-cyclodextrin 과의 포접화합물에 관한 연구

최윤수,김민숙 대구효성가톨릭대학교 1998 연구논문집 Vol.57 No.2

Inclusion complex of pefloxacin(PFX) with β-cyclodextrin(β-CyD) in aqueous solution and in solid phase was studied by solubility test, UV-and IR-spectrometry, melting point test and powder X-ray diffractometry. The stable solid inclusion complex of PFX-β-CyD was prepared by freeze-drying method and molar ratio of the inclusion complex(1:1) was obtained from elemental analytical data. The apparent stability constant k of the inclusion complex was calculated from the solubility diagram by Higuchi equation. Photodegradation studies of PFX and PFX-β-CyD inclusion complex were performed by photochemcial reactor with UV-B light source. Kinetic Photodegradation pattern of PFX and PFX-β-CyD inclusion complex were found as second-order reaction, but photodegradation rate of PFX-β-CyD inclusion complex was decreased than that of PFX alone.

혈소판 Cyclic Nucleotide, Inositol Trisphosphate 및 [Ca^(++)]_(i)의 응집반응성 변동에 대한 항우울성 약물의 영향

최상현,박석산,이민수,전보권,천연숙 고려대학교 의과대학 1992 고려대 의대 잡지 Vol.29 No.3

The pharmacodynamic mechanisms of available antidepressant drugs have not yet clarified. Major depression is common and may more frequently occur for the first time in the eldery, and the incidence of thromboembolism is increased with advancing of age. And many studies have shown an usefulness of platelet as models of monoaminergic neurons. Therefore, this paper. deals with the anti-platelet activity of antidepressant drugs in view of the common action in platelet signal-transduction system of three antidepressants showing different modes of action. The IC_(50)s of amitriptyline alprazolam. and rolipram were 1.39×10^(-4), 2.63×10^(-4), and 8.11×10^(-4)M. respectively, and that of each of the reference drugs : sodium nitroprusside. chlorpromazine. and spermine were 1.26×10^(-6), 5.76×10^(-5) and 4.28 ×10^(-3)M. Rolipram and alprazolam produced the significant increase of platelet cGMP level with slight cAMP increase. showing their selectivity on typeⅡ cGMP-specific PDE. Spermine and antidepressants, particularly used in this study induced the marked increase of platelet IP_(3) level with the anti-platelet and [Ca^(++)]_(i),-decreasing activities. These findings suggest that the mechanism of action of antidepressant drugs seems to be related with their enhancing property of the neuronal IP_(3)-mediated signalling. and that the paradoxical mode of their actions on platelet metabolisms of [Ca^(++)]_(i) and IP_(3) may be a clue of the uncovering of a new cellular [Ca^(++)]_(i)-regulating mode.

77K에서 SC(NH₂)₂의 ¹⁴N 핵사중극 공명연구

최숙자,박영민,박현진,전인,송승기,서용문 명지대학교 자연과학연구소 1997 자연과학논문집 Vol.16 No.-

강유전체 질소화합물 thiourea [SC(NH₂)₂]분말시료의 ??plused NQR 실험을 77K 에서 실행하여 공명진동수와 핵사중극 결합상수, 비대칭인자, 완화시간등을 측정했으며, 4개의 공명선이 관측되었다. 결정내에는 주위 환경이 다른 두 종류의 질소 N₁,N₂가 존재함을 알 수 있었다. N₁에 관한 공명진동수는 ??(1)=2.6487㎒, ??(1)=2.032㎒, N₂에 대하여는 ??(2)=3.1852㎑, ??(2)=2.0183㎒이고, 공명선폭은 ??(1)=2.6752㎑, ??(1)=3.1852㎑, ??(2)=3.2618㎑, ??(2)=3.5448㎑ 임을 보였다. 한편 e²qQ/h의 값은 ν(1)에서 3.1205㎒, η는 0.3953이었으며, ν(2)에서는 3.0279㎒, 0.3939이었다. 이는 이미 보고된 결과와 일치하였다. 그리고, FID(Free Induction Decay)와 spin-echo실험을 통하여 얻은 스핀-격자 완화시간 T₁은 ??(1)에서 7.143 s, ??(2)에서 5.88 s이었으며, 스핀-스핀 완화시간 T₂는 ??(1)에서 25㎳, ??(2)에서 33.3㎳, ??(2)에서 33.3㎳의 값을 얻었다. We have measured ??N NQR frequencies, nuclear quadroupole coupling constants, asymmetric parameters and relaxation times in ferroelectric nitrogen thiourea [SC(NH2)2] at 77K, and four NQR resonance lines were observed. The results of measurements show that there are two kinds of nitrogen site, N1 and N2, with different environments in the crystal. The resonance frequencies were found to be ν+(l)=2.64 87MHz, ν_(1)=2.032 MHz for N1 and ν+(2)=2.6285MHz, ν_(2) =2.0183MHz for N2 respectively. The corresponding linewidths were ν+(1)= 2.6752kHz, Δν_(l) =3.1852kHz, ν+(2) =3.2618kHz and Δν_(2) =3.5448kHz, respectively. For N1, e2Q/h is 3.1205 MHz and η is 0.3953, and for N2, these are 3.0979MHz and 0.3939. These results are in agreement with those reported previously. The spin-lattice relaxation time (Ti) and the spin-spin relaxation time (T2) by FID and spin-echo experiments are as follows : T1(ν+(1)) is 7.143 s and T1(ν+(2)) is 5.88 s. T2(ν+(1)) is 25 ms, T2(ν_(1)) is 25 ms, T2(ν+(2)) is 33.3 ms and T2(ν_(2)) is 33.3 ms.

의복행동과 생활적응사이의 관계연구

김재숙,최민숙 충남대학교 1985 忠南科學硏究誌 Vol.12 No.2

The study was designed (1) to examine interrelationships among clothing variables of Creekmore's clothing importance scale, and (2) to compare the result with Gurel's study, and (3) to find out any significant relationships between clothing variables, and social and intersexual adjustments of high school girls. The following results were obtained by analyzing the data obtained from 497 high school girls in Daejoen area. (1) There were significantly close relationships among clothing variables except comfort which seem to indicate lower construct validity. (2) The interrelationships among clothing variables were compared with the result of Gurel's study ; 6-relationships, in which aesthetic and modesty were two major factors, showed remarkable differences. (3) Intersexual adjustment showed higher relationships with clothing variables than social adjustment. And dependence, interest, aesthetic and attention were major clothing variables which seemed to have more closely related to adjustments:

농촌지역 노인들의 우울증상에 관련된 요인들의 특성

송미숙,전기홍,송현종,김정은,박승구,신상건,이동훈,정성현,최연주,최완철,최민규,황재철,홍지만 아주대학교 1997 아주의학 Vol.2 No.2

This study was performed to identify risk factors for depression among the elderly in a rural area. A survey was done for the elderly living in Gosam-myenn, Ansung-kun during the period of July 28, 1997 and July 30, 1997. Demographic and sodoeconornic characteristics, and functional and health Status were assessed using a structured questionnaire, and the level of depression was measured by GDS score. The data obtained were analyzed by frequencies, percentage, t-test, ANOVA, and multiple regression, and the results were as follows: 1. The total sample consisted of 133 elderly. Of them, 47 elderly (35.4%) showed significant depressive Symptoms. 2. The GDS score was the highest for the group over 85 years of age (p < 0.05). Those living alone or widowed showed lower GDS score than those living with someone or married (p < 0.05). 3. The GDS score was the highest in the absolutely dependent group, followed by the partially dependent group, and the independent group (p < 0.01). The elderly who perceived their health Status as excellent showed the lowest GDS score. In contrast, those who had more than five chronic diseases showed the highest GDS score. 4. The elderly receiving financial and emotional support from their family or friends showed lower GDS score compared to those without any support. 5. The economically dependent group showed higher GDS score than that of independent group. 6. The elderly who involved in leisure activities showed lower GDS score than those without any involvement. 7. In terms of risk factors for depression, we found that perceived health Status, subjective economic Status, leisure activities, living arrangement and financial support were inversely associated with GDS score, whereas labor support was positively associated with GDS score. Based upon the above findings, this study suggests that these risk factors for depression in the elderly should be taken into consideration for a comprehensive mental health program for the elderly living in the rural area.

Tributyl Phosphate와 질산염을 이용한 희토류원소의 용매추출 효과에 관한 연구

이정민,김숙영,최범석 경희대학교 자연과학종합연구원 2000 자연과학논문집 Vol.6 No.-

ICP-AES으로 희토류원소의 분석시 스펙트럼의 방해영향이 없는 분석을 시도하기 위하여 13종의 희토류 원소를 tributyl phdspate로 용매 추출하고 물로 역추출하고, 추출시 희토류원소의 추출효율을 조사하였다. 용매추출시 분배계수를 증가시키기 위하여 NaNO_(3) 농도에 따른 영향과, 소수성 용매의 첨가시 추출 효율을 조사하였다. NaNO_(3) 농도가 증가할수록 추출효율은 증가하며, 4M 이상에서 몇가지의 원소를 제외하고 추출효율이 95% 이상으로 높았다. 반면에 소수성용매의 첨가는 추출효율을 오히려 감소시켰다. Group separation of 13 rare earth elements(REE) was achieved by solvent extraction with tributyl phsphate, followed by back extraction with water in order to separate the REE from the matrix. The method can circumvent the spectral interferences for ICP-AES. Effects of the NaNO_(3) concentration and addition of hydrophobic solvents on the extraction efficiencies are investigated. Increases of the NaNO_(3) concentration enhanced the extraction efficiencies of REE and more than 95% recovery was obtained above 4 M of NaNO_(3) concentration. On the other hand, addition of hydrophobic solvents lowered the extraction efficiencies.

두경부 편평세포암종세포주에서 retinoic acid가 linear-quadratic 모델을 적용한 방사선감수성과 apoptosis 유발에 미치는 영향

이은숙,강범현,허민석,이삼선,최현배,최순철,박태원 대한구강악안면방사선학회 2001 Imaging Science in Dentistry Vol.31 No.3

Purpose : To evaluate the effect of all-trans-retinoic acid on radiosensitivity and radiation-induced apoptosis in NHOK, HEp-2 and FaDu cell lines. Material and Methods : We measured the changes in survival fraction at 2 Gy (SF2), α and β after treatment of retinoic acid (1μM) prior to irradiation with doses of 2,4,6 and 10 Gy and correlated the radiosensitizing effect of retinoic acid with them. Also, apoptosis induction was assayed with the flow cytometry on days 1, 2, 3, 4 and 5 after irradiation (2,10 and 20 Gy) combined with retinoic acid. Results and Conclusion : SF2 values for NHOK, HEp-2 and FaDu cell lines were 0.54, 0.64 and 0.41, respectively and the cell line of FaDu was the most radiosensitive, For cell lines of NHOK and HEp-2, pretreatment of cells with retinoic acid resulted in a significant decrease of the SF2 values. The α/β ratios of x-ray survival curve were 8.714(NHOK), 4.098(HEp-2) and 11.79(FaDu). The α/β ratio for NHOK decreased on pretreatment with retinoic acid, whereas those for HEp-2 and FaDu increased. Radiation induced apoptosis in all cell lines but, retinoic acid did not affect the apoptosis. (Korean J Oral Maxillofac Radiol 2001; 31 : 135-43)