Effect of n-Butyrate on the In Vitro Reactivation of Latent Herpes Simplex Virus

천연숙,박노희,Chun, Yeon-Sook,Park, No-Hee The Korean Society of Pharmacology 1986 대한약리학잡지 Vol.22 No.2

4-carbon fatty acid인 n-Butyrate(n-BTA)는 herpes virus의 일종인 Epstein-Bat virus(EBV)에 작용해서 잠복형인 EBV를 활성형태로 유도시키는 것으로 알려져 있다. 고로 본 실험에서는 mouse의 삼차신경절에 잠복하고 있는 HSV의 재활성화에 대한 n-BTA의 효과를 실험 관찰하였다. Pentobarbital로 마취시킨 mouse의 양쪽눈 각막을 30 gauge 주사바늘로 scarify한 후에 type I HSV(HSV-1) $10{\mu}1(1{\times}10^5$ plaque-forming units)를 각각 점안 감염시켰다. virus를 감염시킨 4주 후에 mouse의 삼차신경절을 적출하여 시험관 내에서 조직배양을 시행하였다. 조직배양시에 0. 1, 0. 25, 0.5. 1.0 그리고 2.0mM농도의 n-BTA를 첨가하였으며 1일, 2일, 3일간 각각 배양한 후 신경절을 연마하여 연마액내의 HSV-1 titer를Vero cell monolayer에서 plaque assay로 측정하였다. 1) n-BTA첨가군은 잠재성 HSV가 대조군에 비하여 현저하게 빨리 재활성화 되었고 재활성화되는 virus의 양도 현저히 증가되었다. 2) 24시간을 계속해서 n-BTA 각 농도를 첨가해서 배양할 군은 n-BTA 6시간 첨가 배양하고 새로운 배양액으로 갈아서 18시간 배양한 군에 비해 잠재성 virus의 재활성화가 현저히 증가되었다. 3) Gang1ionic latent HSV-1의 재 활성화에 영향을 미치는 각 농도의 n-BTA는 Vero cell의 monolayer에서의 HSV-1의 번식에는 아무런 영향을 미치지 않았다. n-Butyrate (n-BT A) increased the rate and number of infectious units produced in the in vitro reactivation of latent herpes simplex virus. While the mechanism of action of n-BT A is obscure, a continuous presence of n-BT A is necessary for its inductive effect.

Sodium fluoride 의 作用機轉에 對한 硏究

千然淑 고려대학교 의과대학 1973 고려대학교 의과대학 잡지 Vol.10 No.2

摘出臟器에 對한 營養液成分으로서의 Magnesium 및 Manganese의 影響의 比較實驗

千然淑,申萬鍊 우석대학교 의과대학 1968 우석의대잡지 Vol.5 No.1

A study has been made on the effect of manganese chloride or magnesium chloride in Locke-Ringer solution on the contractile action of acetylcholine and ouabain in smooth muscle. The organs used for experiment were the excised atrium, upper intestine of rabbit and also the matured uterus, upper intestine of the rat The concentration of manganese chloride or magnesium chloride in Locke-Ringer solution was 0.2mM, 0.5mM, and 1.0mM. To investigate the relationship between the cholinesterase activity of homogenized tissue and the contractile force of the excised atrium and intestinal smooth muscle immersed in MnCl₂-, or MgCl₂-Locke-Ringer solution, the author observed the movement of excised organ preparations and measured the cholinesterase activity of homogenized atrium and upper intestine of the rabbit. The results obtained were as follows. 1. As the concentration of MnCl₂ or MgCl₂ in Locke-Ringer solution was increased, the amplitude of the isolated atrium was decreased. This depressive effect was more prominent in MnCl₂ than in MgCl₂-Locke-Ringer solution. The depressive effect of 10^(-7) acetylcholine on the amplitude of the atrial contraction was decreased more markedly in MgCl₂-Locke-Ringer solution than in MnCl₂-.The duration caused atrial standstill by ouabain was prolonged as long as two folds in MgCl₂-, or MnCl₂-Locke-Ringer solution than in control. 2. The sensitivity of excised intestine to acetylcholine was markedly increased in magnesium chloride or manganese chloride Locke-Ringer solution. The sensitivity of excised intestine of the rabbit was augmented more than two folds, in 1.0mM MgCl₂- or MnCl₂-Locke-Ringer solution than those in control, and the sensitivity of rat intestine was increased markedly in 0.2mM magnesium chloride or manganese chloride-Locke-Ringer solution. 3. The sensitivity of excised uterus of the rat pretreated with estrogen 24 hours prior to acetylcholine administration in 0.5mM MgCl₂ or MgCl₂ Locke-Ringer solution. This increased sensitivity was more prominent in manganese Locke-Ringer solution than in MgCl₂. 4. The cholinesterase activity of the rabbit intestine and atrium showed the decreasing tendency, as the concentration of MgCl₂ or MgCl₂ in Locke-Ringer solution increased.

Sodium fluoride 의 作用機轉에 對한 硏究 : Ⅰ. 循環器系統 및 腸管 平滑筋에 對한 實驗 Ⅰ. On the circulatory system and intestinal smooth muscle

千然淑 고려대학교 의과대학 1973 고려대 의대 잡지 Vol.10 No.2

To investigate the acting mechanism of sodium fluoride on the blood pressure, the inotropic effect of isolated auricle and the tonicity of isolated intestine of rabbit, the author observed the catecholamine contents of cardiac muscle of rats after perfusion of Lock-Ringer solution containing various dose of sodium fluoride, and the influence of β-adrenergic blocking agent or the other drugs on the pressure response, the positive inotropic effect of isolated auricle and increased tonicity of isolated rabbit intestine induced by sodium fluoride. The results obtained were follows: 1. Large dose of sodium fluoride stimulated the release of catecholamine from the isolated perfused rat heart. 2. Large dose of sodium fluoride increased the amplitude of isolated rabbit auricle, which augmented effect is decreased by pretreatment of DCI or inderal. 3. Large dose of sodium fluoride rised blood pressure of rabbits, which effect is decreased by the pretreatment of DCI or isoproterenol. 4. The tonicity of isolated rabbit intestine is continuously increased by sodium fluoride, which effect is antagonized by DCI. atropine or papaverine.

항 히스타민제의 약리작용과 투여상의 문제점

천연숙 臨床醫學社 1987 臨床醫學 Vol.1987 No.8

Morphine이 Norepinephrine 代謝에 미치는 影響 : Ⅰ. Morphine이 家兎의 腦, 心臟 및 腎臟의 norepinephrine 含量과 轉換에 미치는 效果 Ⅰ. The effect of morphine on the content and turnover of norepinephrine in the brain, heart and kidney of rabbits

千然淑 고려대학교 의과대학 1979 고려대 의대 잡지 Vol.16 No.2

Numerous studies using a variety of species implicated brain catecholamin in the analgesic activity of morphine. Yet the ability of single doses of morphine to change brain norepinephrine level would appear to be a species dependent phenomenon. This study was performed to observe the effect of morphine on the content and turnover of norepinephrine(NE) in the brain, heart and kidney of rabbits. The results were obtained as follows ; 1. Various doses (5, 10 and 20㎎/㎏) of morphine increased the brain NE and renal NE content and decreased the cardiac NE content 20 minutes after the morphine injection, and these NE changing effects of morphine were dose-dependent. 2. The time courses (20, 60 and 90 min.) of the effect of morphine (10 and 20㎎/㎏) on the NE content in several organs of rabbits showed an initial increase in the brain NE content, acute decrease in the cardiac NE content and gradual increase in the renal NE content. 3. The initial increase of brain NE content induced by morphine was continuously increased by iproniazid pretreatment, but the decreased cardiac NE and increased renal NE content induced by morphine were inhibited by the pretreatment.

항 바이러스 약물의 기전 및 생체동향

천연숙 약업신문사 1997 의약정보 Vol.1997 No.5

Effect of Chemical Carcinogens on the Replication, Cytolyticity, DNA Synthesis, and Protein Expression of Herpes Simplex Virus in Viral Infected Cells

천연숙,Chun, Yeon-Sook The Korean Society of Pharmacology 1992 대한약리학잡지 Vol.28 No.2

Benzopyrene (BP), 7,12-dimehyl benzanthracene (DMBA), nitrosomethyl urea (NUMU) 및 nicotine과 같은 발암성 화학물질들이 바이러스 감염된 vero 세포의 단층 세포 배양에서 I형 단순성포진 바이러스 (HSV-1)의 복제, 세포융해, DNA합성 및 단백질 합성에 미치는 효과를 관찰하였다. 1. BP와 DMBA는 HSV-1의 복제와 세포융해작용을 유의성있게 억제하였으나 nicotine과 NMU는 별로 억제하지 않았다. 2. 모든 발암성 화학물질은 바이러스의 DNA합성을 억제하지 못하였지만 새로 합성되는 후손바이러스 DNA로 부터 표현되는 gamma 단백질의 표현은 BP와 DMBA에 의해서 현저하게 억제되었다. 그러나 모든 발암성 화학물질은 바이러스의 alpha 및 beta 단백질의 합성은 억제하지 못하였다. 이상의 결과로 보아 발암성화학물질이 존재하고 있는 배지내에서 새로 합성되는 바이러스의 DNA로 부터 표현되는 gamma 단백질의 결함이 있음을 알 수가 있었으며 이같은 개념은 발암화학물질의 존재하에서 바이러스의 DNA와 단백질이 거의 정상적으로 합성됨에도 불구하고 바이러스의 복제가 일어나지 않는다는 사실이 뒷받침해주고 있다. We investigated effects of several chemical carcinogens, i.e., $benzo({\alpha})pyrene$ (BP),7,12-dimethylbenz(a)anthracene (DMBA), nitrosomethyl urea (NMU), and nicotine on the replication, cytolyticity, DNA synthesis, and protein synthesis of type 1 herpes simplex virus (HSV-1) in viral infected Vero cell monolayers. We observed that the BP and DMBA did not show such activity. All chemical carcinogens did not inhibit the synthesis of viral DNA, but the expression of gamma viral proteins that are expressed from the newly synthesized progeny viral DNA was somewhat notably inhibited by BP and DMBA. However, the synthesis of alpha and beta viral proteins was not altered by the chemical carcinogens. These data indicate that the gamma viral proteins expressed from the newly synthesized DNA in the presence of chemical carcinogens in the culture medium may be defective. This is further supported by the fact that the virus fail to replicate in the presence of these chemical carcinogens, in spite of viral DNA and proteins are somewhat normally synthesized.

Guanethidine이 白鼠摘出心臟의 Catecholamine 遊離에 미치는 效果

千然淑 고려대학교 의과대학 1975 고려대 의대 잡지 Vol.12 No.2

The isolated heart of matured rat in the perfusion experiments respond to continuous infusion of guanethidine with an increase in heart rate. This agent appear to increase heart rate by releasing endogenous norepinephrine, since depletion of cardiac norepinephrine store abolishes the action. In the present studies, to inverstigate the role of guanethidine on the release of catecholamine, the author observed the influence of guanethidine on the variations of catecholamine contents in the myocardium and perfusate from the perfusion experiment of isolated rat heart. Perfusions were carried out with varying concentration(1㎎, 5㎎ and 10㎎ per 1L of Tyrode solution) of guanethidine for 1 hour, 2 hours and 3 hours, according to Langendorff method. The results were as follows; 1. The norepinephrine content of isolated myocardium was decreased by the perfusion of guanethidine. 2. The norepinephrine content in the perfusate from isolated myocardium was increased by the perfusion of guanethidine. 3. Total norepinephrine content in both of isolated myocardium and the perfusate from isolated myocardium was increased progressively by the perfusion of increasing concentration of guanethidine in Tyrode solution and the prolongation of perfusion.

Morphine이 Norepinephrine 代謝에 미치는 影響

千然淑 고려대학교 의과대학 1979 고려대학교 의과대학 잡지 Vol.16 No.2