소 질병의 혈액화학적 연구

김덕환,이교영,이경갑,최희인,오사메 사토시,이찌조 시게루 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.1 No.-

In prder to clarify the changes of serum chemical values(AST, CPK and LDH activities)and LDH isoenzyme fractions in frequent occuring some diseases of dairy cows, total 48 cows(40 patients: 18 with downer cow syndrome, 17 with abomasal displacement and 5 with acute mastitis and 8 controls: clinically healthy cows) were examined. The results obtained were as follows: 1. In changes of total serum enzyme activities of patients group, increase of serum AST(P<0.001), CPK(P<0.01) and LDH activities(P<0.01)were observed in groups of downer cow syndrome and abomasal displacement, respectively by comparison with control group. Increase of AST only was found in group of acute mastitis(P<0.05). 2. In LDH isoenzyme fractions, increase of LDH₁(P<0.01), LDH₂(P<0.05), LDH₃(P<0.01), LDH₄(P<0.01) and LDH_5(P<0.01)fractiona was observed in group of downer cow syndrome and also marker increase of LDH4 and LDH5 fractions was noted in this group. The increase of LDH₁, LDH₂ amd LDH₃ (P<0.01)fractions was found in group of abomasal displacement, but group of acute mastitis showed similar result to that of control group.

Differential Diagnosis of Thyroid Follicular Neoplasm from Nodular Hyperplasia by Shear Wave Elastography

Myung Hi Yoo,Hye Jeong Kim,In Ho Choi,Ji-Oh Mok,Hyeong Kyu Park,Dong Won Byun,Kyoil Suh 순천향대학교 순천향의학연구소 2019 Journal of Soonchunhyang Medical Science Vol.25 No.1

Objective: More than 50% of diagnostic surgery in cytologically indeterminate thyroid nodules revealed benign nodules of which nodular hyperplasia (NH) accounted for about half. Preoperative exclusion of NH may decrease diagnostic surgery. We aimed to study the diagnostic performance of shear wave elastography (SWE) to differentiate follicular neoplasm (FN) from NH in follicular lesions of thyroid nodules. Methods: We analyzed the data from 61 patients who underwent SWE before ultrasound-guided core-needle biopsy (CNB) from August 2016 to May 2018 and CNB results were FN (n=19) and NH (n=42). We analyzed the magnitude of elasticity index (EI) and patterns of high EI area (EI >36 kPa). The patterns of high EI area was classified as marginal pattern (high EI areas are restricted in the outer 1/3 of the nodule) and traversing pattern (high EI areas approaching further to the center of the nodule within inner 2/3 of the nodule). Results: The EMax, EMean, ESD, and percent of high EI area were significantly lower in FN than NH (P<0.001). The diagnostic performance to predict FN showed sensitivity, specificity, and accuracy of 95%, 90%, and 92% by EMax <42.1 kPa, and of 95%, 79%, and 84% by EMean <23.5 kPa, and of 89%, 93%, and 92% by marginal pattern of high EI area, respectively. Conclusion: Novel diagnostic criteria of EMax less than 42.1 kPa and marginal pattern of high EI area on SWE can predict FN with high diagnostic accuracy, waiving diagnostic surgery of NH in indeterminate cytology.

줄넘기 운동이 과체중 남자중학생의 건강체력에 미치는 영향

구인권,최희남,김현수 한국학교체육학회 1999 한국학교 체육학회지 Vol.9 No.1

본 연구는 8주간의 줄넘기운동이 남자중학생 비만자의 건강관련 체력변인(근력, 근지구력, 유연성, 전신지구성, 신체구성)에 미치는 영향을 검토하기 위해 체지방율이 25%가 넘는 학생 20명을 운동집단(10명)과 통제집단(10명)으로 분류하여 매주 4회, 6-12분/1set, 그리고 분당 120회의 줄넘기 운동을 138박자 음악에 맞춰 1인용 구름판 위에서 4세트를 실시하였고, 세트당 휴식은 2분간 동적인 휴식을 실시하여 측정한 결과 운동집단에서 체중(1.5kg)과 신체 둘레의 가슴둘레(3.4mm)와 복부둘레(1.4mm), 그리고 체지방률(2.8%)이 모두 유의하게 감소하였다(p<.001), 근기능의 평가항목인 악력(2.6kg)과 아령들기(2.4회/30초)도 유의하게 증가하였으나 통제집단 역시 증가하여 양 그룹사이에서 유의한 차이를 발견하지 못하였다. 반면 하지 근지구력의 측정항목인 앉았다 일어나기는 운동그룹에서 유의한 증가를 보인 반면 (p<.001), 통제집단에서는 유의하지 않았다. 유연성을 평가한 앉아 윗몸 앞으로 구부리기는 운동집단이 9.1에서 10.3cm로 증가하였고(P<.05), 통제집단에서는 유의한 것은 아니나 오히려 약간 감소하였다. 이상과 같이 하지근지구력을 제외한 항목에서는 프로그램 후의 측정값에서 운동그룹과 통제그룹사이에서 유의한 차이를 보이지 않았다. 운동군에서 1600m 걷기로 추정한 최대산섭취량 역시 53.5에서 56.3 mℓ/kg/min으로 유의한 증가(p<.001)를 보였다. 그리고 운동에 참가한 모든 학생이 무릎이나 허리의 통증을 호소한 사람은 한 사람도 없었다. 이상과 같이 음악에 맞추어 실시한 구름판 줄넘기 운동프로그램이 비만 남자중학생의 신체 구성을 포함한 건강관련 체력의 향상과 정형외과적 상해방지에 유효하다는 것을 알았다. The purpose of this study was to investigate the effect of the 8 week rope skip exercise program on the health-related fitness for overweight boys (13.6±0.4 years). Twenty boys were randomized into two groups: nonexercising control (C, n=10) and ouexercise (Ex, n=10) groups, The Ex. group had rope skipping four times a week during the 8 weeks (120 skippings/min) with 138 beat music on the spring board After rope skipping program, the difference of the variation is verified by one-way ANOVA and paired t-test and the significance level is set up to .05. There were significant decrease in the percentage body fat and total fat mass in Ex group. And there was significant improvement in the cardiorespiratory fitness and muscular fitness following 8 week of training (p<.001). The results suggest that the rope skipping with music on the spring board is an effective exercise for overweight boys..

Medicinal Chemistry : Article ; Acredinones A and B, Voltage-dependent Potassium Channel Inhibitors from the Sponge-Derived Fungus Acrmonium sp. F9A015

( Hi Young Kim ),( In Ho Yang ),( Shin Young Ryu ),( Dong Hwan Won ),( Awadut G. Giri ),( Wei Hong Wang ),( Hyuk Jae Choi ),( Jung Wook Chin ),( Dong Yup Hahn ),( Eun Hee Kim ),( Chul Kyeong Han ),( J 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-

Two new benzophenones, acredinones A(1) and B(2), were isolated from a marine-sponge-associated Acre-monium sp. Fungus. Their chemical structures were elucidated on the interpretation of spectroscopic data. The structure of 1 was confirmed by palladium-catalyzed hydrogenation, followed by spectroscopic data analysis. Acredinones A (1) and B (2) inhibited the outward K currents of the insulin secreting cell line INS-1 with IC50 values of 0.59 and 1.0 um, respectively.

Development of a mixed-mode chromatography with tandem mass spectrometry method for the quantitative analysis of 23 underivatized amino acids in human serum

Choi, Min Sun,Rehman, Shaheed Ur,Kim, In Sook,Park, Hi-Joon,Song, Mi-Yeon,Yoo, Hye Hyun Elsevier 2017 Journal of pharmaceutical and biomedical analysis Vol.145 No.-

<P><B>Abstract</B></P> <P>In this study, a robust, selective and simplified method was developed and validated for the simultaneous quantitative analysis of 23 underivatized amino acids in human serum using mixed-mode chromatography with tandem mass spectrometry (LC–MS/MS). Serum samples were deproteinized with acetonitrile and subjected to LC–MS/MS analysis. The chromatographic separation of amino acids was achieved using a mixed-mode column (150×3mm, 3μm) with a gradient elution system; the mobile phase consisted of 50mM ammonium formate and 0.1% formic acid in acetonitrile. The total run time was 22min. Eluted compounds were detected in the electrospray ionization-positive mode with multiple reaction monitoring. The validation study evaluated linearity, repeatability, intra and inter-day accuracy and precision, and matrix effect. The validation results were satisfactory in all the tested parameters. This method was successfully applied to the analysis of amino acids in the clinical sample of human serum.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A simultaneous quantitative method for underivatized amino acids in human serum was developed. </LI> <LI> In this method, mixed-mode chromatography with tandem mass spectrometry was used. </LI> <LI> The analytical method validation was properly performed. </LI> <LI> This method was applied to the analysis of amino acids in the clinical samples of human serum. </LI> </UL> </P>

한국 재래 염소의 성장에 따르는 혈액상의 변동

최희인,Choi, Hi In 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.1

Although considerable research has been done on the changes associated with age in the blood picture of domestic and laboratory animals, little work has been made of the changes occurring at different age in the blood picture of goats. And a comprehensive survey of the bood picture of Korean native goats has not been made. The object of the present investigation was to suggest standards for the blood picture of Korean native goats at frequent intervals from birth to maturity. The goats were kept under average farming conditions in this country. Observations were made at the following ages: at birth; 2,4 and 7 days; 2, 3, 4, 5, 6, 7, 8 and 9 weeks; 2.5, 3,6 and 12 months. Blood samples were drawn from the jugular vein. It was taken between 8 and 9 a.m. except those for the at-birth period. Erythrocyte and leukocyte enumerations and, determinations of hemoglobin in blood and hematocrit value were made in the usual manner. Reticulocytes were enumerated per 1,000 erythrocytes in blood smears stained with briIliant cresyl blue and counterstained with Wright's stain. Erythrocytes counts declined from $8.7{\times}10^8/mm^3$ at birth to a low of 7.0 at 4 days of age. These values increased to 11.5 at 5 weeks and reached a maximum of 14.0 at 3 months of age; it then fell to 11.5 at 12 months of age. Concentrations of hemoglobin in blood and hematocrit values were not related to the changes of erythrocyte counts. The values at birth were higher than at any other period during the first year of life. These fell from highs of 12.3 g/100 ml and 38.0 ml/100 ml to lows of 9.2 and 29 at 4 weeks for concentration of hemoglobin in blood and hematocrit value, respectively. There was a common pattern for the hematocrit value and hemoglobin in blood which showed three phases-a fall during the first month, a rise to the third month, and a fall to the mature level at 12 months of age. Mean corpuscular volume and mean corpuscular hemoglobin showed a common pattern. The values were $44.2{\mu}m^3$ and 14.2 pg at birth and fell, at first slowly and then rapidly, to reach adult levels of 24.1 and 7.9 at 6 weeks of age for mean corpuscular volume and mean corpuscular hemoglobin, respectively. Mean corpuscular hemoglobin concentration was little affected by age. Reticulocyte was observed from birth to 4 weeks of age. Percentage of reticulocyte decreased from 0.85% at birth to 0.06% at 4 weeks of age. Total leucocyte counts increased from $5.64{\times}10^3/mm^3$ at birth to a maximum of 13.4 at 3 months; it then fell to 11.5 at 12 months of age. In differential counts myelocyte, juvenile and band form decreased with advancing age. No myelocyte and juvenile were seen after the age of 7 and 9 weeks, respectively, and band forms were rare after the age of 3 months. Percentage of mature neutrophil showed a quick decline from 52.5% at birth to reach a minimum level (34.5%) at 3 months of age; it then rose to 38% at 12 months of age. Percentage of lymphocyte increased from 39.2% at birth to maximum of 59% at 3 month of age; it then fell to 54.9% at 12 months of age. Percentage of monocyte was not affected with advance of age. Percentage of eosinophil and basophil were increased with advance of age to reach a maximum at 2 to 3 month of age. It then fell to adult level at 12 month of age.

위암에서 에스트로젠 수용체 알파의 소실과 프로모터 과메틸레이션

우인숙,문도호,김성훈,임소희,이명아,강진형,홍영선,이경식,최명규,정인식,박경신 대한내과학회 2004 대한내과학회지 Vol.66 No.5

목적 : 위암에서 에스트로겐 수용체 알파의 프로모터 부위의 메틸레이션으로 인한 위암 조직 내 발현소실이나 불활성화에 대해서는 아직 보고된 바가 없다. 본 연구에서는 위암 세포주와 위암으로 진단 받은 환자들의 조직에서 에스트로젠 수용체 알파의 발현을 평가한 후 위암에서 에스트로젠 수용체 알파의 소실이 5' 프로모터 부위의 메틸레이션과 관련이 있는지를 알고자 하였다. 방법 : 위암 세포주에 대하여 에스트로젠 수용체 알파의 발현을 보기 위하여 웨스턴블롯(12개 세포주)과 RT-PCR(13개 세포주)을 시행하였다. DNA에 대하여 bisulfite modification을 하여 메틸레이션 특이적 중합효소 연쇄반응과 TA 클로닝 후에 염기서열 분석을 시행하였다. 위내시경 조직검사에서 위암으로 진단 받은 42명의 위암 환자의 조직에 대하여 에스트로젠 수용체 알파에 대한 면역조직 화학검사를 시행하였다. 결과 : 위스턴블롯과 RT-PCR결과 KatoⅢ 세포주의 mRNA만 약하게 발현되었으며 모두 음성 반응을 보였다. 메틸레이션 특이적 중합효소 연쇄반응에서 모두 양성이었다. 염기서열 분석에서 ATG 시작 코돈 주변의 CpG island의 methylation을 확인할 수 있었다. 결론 : 위암에서 에스트로젠 수용체 알파의 ATG 시작 코돈주변의 프로모터 부위의 CpG 부위에서 관찰된 메틸레이션은 ER-α유전자의 소실을 일으킬 수 있는 원인의 하나가 될 수 있을 것으로 생각된다. Background : The significance of ER expression and hormone manipulation in gastric cancer is not established. There have been several reports supporting the role of the ER gene as tumor suppressor gene in carcinogenesis. The ER-α gene is located on chromosome 6q25.1. Deletions of the long arm of chromosome 6 are common in gastric carcinoma, suggesting the pressence of tumor suppressor genes in this region. The proportion of ER-positive gastric cancers ranges between 0% and 67% depending on the method of detection. Epigenetic inactivation might explain the loss of ER-α gene expression in gastric cancer. There is no information available regarding the methylation status of the ER-α gene promotor region in gastric cancer so far. The aim of this study was to assess the expression of ER-α in gastric cancer cell lines and determine whether methylation of the 5' promotor region is associated with loss of ER-α expression in gastric cancer. Methods : We investigated such methylation in 13 gastric cancer cell lines. Western blot analysis, reverse transcription-polymerase chain reaction (PCR), methylation-specific PCR (MS-PCR) and bisulfite sequencing analyses were used. Immunohistochemical staining for the ER-α gene was dome for forty-two paraffin embedded tissues from gastric cancer patients. Results : ER-α protein was not detected in any cell line, ER-α mRNA was expressed in only Kato Ⅲline. MS-PCR and bisulfite sequencing showed all thirteen gastric cancer cell lines had methylated CpG regions in their ER-α gene promoters. Immunohistochemical staining of ER-α showed no positivity in any of examined samples. Conclusion : Inactivation of ER-α gene expression in gastric cancer cell lines appears associated with CpG island methylation near the TGA initiation codon of the ER-α gene.

호흡곤란 평가지표들의 비교

정익주(Ik Joo Chung),최인선(In Seon Choi),박상선(Sang Seon Park),고영일(Young Il Koh),이민수(Min Su Lee),강유호(Yu Ho Kang),이신석(Shin Seok Lee),박재희(Jae Hi Park),박경옥(Kyung Ok Park) 대한내과학회 1993 대한내과학회지 Vol.45 No.5

N/A Background: Although the pulmonary function tests have been widely used as the methods to investigate the dyspneic patients with respiratory disease, it has often been found that the correlation between the lung function and the degree of subjective sense of dyspnea is not so good. And so, various methods of categorizing subjective assessment of dyspnea severity have been developed, and this study was conducted to determine the usefulness of these methods in the evaluation of dyspneic patients with respiratory diseases. Methods: Clinical or psychophysical dyspnea indices {modified Medical Research Council (MRC) scale, Oxygen-Cost Diagram (OCD), Baseline Dyspnea Index (BDI), modified Borg category scale (Borg) and the distance walked in 12 minutes (12MW)} and pulmonary function were measured in 29 consecutive patients with respiratory diseases, who were referred to the clinical pulmonary function laboratory of Chonnam University Hospital from June 1991 to September 1992. Results: The ratings for the modified MRC, OCD, BDI, and Borg, and 12 MW were related significantly each other. However, the relationship between lung function and dyspnea indices were weak. The correlation between the results of two attempts of 12 minute walking test in individual patients was high(r=0.94, p<0.001) but the distance walked on the second occasion was significantly greater(p<0.05). In 10 patients rested at least once during their attemt the distance to the first stop correlated well with the total distance covered in 12 minutes(r=0.91, p<0.005). Conclusion: These resu1ts suggest that the various methods for rating dyspnea are interrelated and reproducible, but those were related weakly to lung function and may yield the independent useful information. And the use of the distance to the first stop in 12 minute walking test may not compromise the value of the test in patients with severe disability.

장결핵 생검조직을 이용한 병리학적 진단 방법에 관한 연구

김경미,이안희,심상인,최규용,오세정 대한병리학회 1997 Journal of Pathology and Translational Medicine Vol.31 No.8

비궤양성 소화불량 환자에서 위점막 산도와 Helicobacter pylori 와의 관계 : phenol red 액을 이용한 색소 내시경

김영신,최규용,정인식,선희식,박두호,한석원,윤종구,채현석,장성원,이안희 대한소화기내시경학회 1997 Clinical Endoscopy Vol.17 No.5

Background/Aims: The Helicobacter pylori(H. pylori) infection induces gastric mucosal injury through the various bacterial cytotoxins, the inflammatory reaction of the host and the increased gastric acid secretion. Urease is a kind of adaptive protein of H. pylori to survive in strong acid environment of the stomach, may increase the Ph of the gastric mucosal surface and induces gastrin release by the feedback mechanism. This study was performed to evaluate whether 0.1% phenol red solution without urea is useful as a Ph indicator of the gastric mucosa for the diagnosis of H. pylori infection in stomach and whether the Ph of the gastric mucosal surface is changed by H. pylori infection. Methods: The gastric mucosa was stained by 0.1% phenol red solution without urea during endoscopy in 89 patients with non-ulcer dyspepsia. The patterns of staining of the gastric mucosa were divided into unstained, patchy regional and diffuse staining by the area of color change from yellow to red. The Ph of the gastric mucosal surface was measured directly on the stained & unstained areas of the gastric mucosa by using Ph meter and antimony Ph electrode through the biopsy channel. Results: The Ph of the stained areas after spray of phenol red solution was significantly higher(6.9) than that(1.9) of the unstained gastric mucosa(P$lt;0.01). The patterns of the staining were different between antrum and body. The patterns of unstaining and patchy staining were more common in the body than in the antrum. But the patterns of regional and diffuse staining were more common in the antrum than in the body(P$lt;0.05). The positive rates of H. pylori in antrum, body & total gastric biopsies were higher in stained than in unstained mucosa significantly(P$lt;0.05). Severity of active inflammatory reactions was higher in stained mucosa than unstained mucosa in the antrum. But there was no difference in severity of active inflammatory reactions between stained mucosa and unstained mucosa in the body. Conclusions: 0.1% phenol red solution without urea is useful as a Ph indicator for the diagnosis of the H. pylori infection in the stomach. H. pylori infection may increase the Ph of gastric mucosal surface and induce severe active inflammation of the gastric mucosa in non-ulcer dyspepsia.