바베시아 원충감염 예방과 치료에 대한 비타민 E의 응용

이경갑,김근형,이영재,Lee, Kyoung-kap,Kim, Geun-hyoung,Lee, Young-jae 대한수의학회 1999 대한수의학회지 Vol.39 No.5

This study was carried out to investigate the effects of vitamin E on the prevention and treatment of Babesia gibsoni. Fifteen mongrel dogs, uninfected with Babesia spp, were assigned to three groups according to vitamin E(${\alpha}-tocopherol$) concentrations in the RBC. The concentrations in each of the three groups were, respectively : ${\alpha}-tocopherol$ in RBC less than $30{\mu}g/{\mu}l$(Group I), $30{\mu}g/{\mu}l{\sim}60{\mu}g/{\mu}l$(Group II), more than $60{\mu}g/{\mu}l$(Group III). Artificial infection was accomplished by injecting $2{\times}10^7{\sim}2{\times}10^8$ erythrocyte of Babesia gibsoni-infected dog into the cephalic vein. We investigated the clinical signs, vitamin E concentrations in RBC and serum, Vitamin A concentrations in serum, hematological values, white blood cell(WBC) viability and RBC membrane osmotic fragility after infection of Babesia gibsoni for a period of 20 days at 5 day intervals. The results obtained are summarized as follows : 1. After infection by Babesia gibsoni, clinical examination revealed depression, anorexia, pale mucous membranes, dark brown urine and diarrhea in proportion as time went on. After 10 days of infection, one dog each of Groups I, II and III revealed depression and anorexia. Two dogs in Group I and one dog each of Groups II and III showed dark brown urine after 15 days. Diarrhea was observed in one dog in each of the 3 groups after 20 days of infection. 2. After 5 days of infection, two dogs in each of Groups I, II and III showed Babesia gibsoni in RBC of blood smear stained with Giemsa. At the 15th day after infection with Babesia gibsoni, they were observed in all experimental animals. After both 5 days and 10 days of infection, the rate of Babesia gibsoni parasitized RBC(permillage, ‰) was 1‰, and increased as time went on. 3. After 5 days of infection by Babesia gibsoni, Group I, which had the lowest vitamin E concentration, showed significantly decreased RBC and PCV levels(p < 0.01). Group II and group III also showed significantly decreased RBC and PCV levels after 15 days of infection(p < 0.05). Particularly after 10 days of infection, Group I showed lower values in RBC and PCV levels compared to Groups II and III. WBC, RBC, fibrinogen and total protein levels between the groups did not differ during experimental periods. 4. According to the WBC differential counts, the ratios of neutrophil to lymphocyte showed a tendency to be slightly higher in Group III (more than $60{\mu}g/{\mu}l$) than in Groups I and II. 5. WBC viability did not differ between the groups. 6. RBC membrane osmotic fragility did not differ between the groups.

개의 피부질환

이경갑 대한수의사회 2000 대한수의사회지 Vol.36 No.6

한국흑염소에 있어서 DL-Ethionine으로 유발시킨 지방간에 관한 연구

이경갑,최희인,Lee Kyoung-Kap,Choi Hee-In 한국임상수의학회 1991 한국임상수의학회지 Vol.8 No.2

This study was carried out to investigate fatty liver in Korean black goats. Adult female goats were divided into 3 test groups(A, B and C). Group A and B of goats each received 3 test consecutive daily doses of DL-ethionine at 75mg/kg and 150mg/kg body weight, respectively. Group C of goats was given 3 consecutive doses of the compound every 48 hours at 150mg/kg body weight. The clinical symptoms, hematological values, serum chemical values and histopathological study of the liver were investigated in the test animals. The results obtained are as follows ; 1. Fatty liver were observed in every test animal. 2. Some clinical symptoms( anorexia, depression) were appeared from 1st day to 7th day after administration of the compound in every test animal. In addition to these symptoms, diarrhea and salivation were generally observed in test animals which were given the compound at 150mg/kg body weight. The degree of these symptoms was dose dependent. 3. There was no significant variations in total WBC counts and fibrinogen values in the blood of test goats. The PCV values were significantly increased on 5th day of dosing in group A and B of goats. 4. The total lipid value was not changed but the concentration of NEFA was significantly increased on 3rd day of dosing with the compound and returned to normal value after 10 days hereafter. The value of triglycerides was significantly increased on 1st day and returned to normal value on 3rd day of dosing. The value of cholesterol was significantly decreased on 3rd day and returned to normal value on 10th day after treatment. 5. Total protein level was decreased on 10th day of dosing in the groups of B and C, and billirubin level was significantly increased on 7th day of dosing in every test group and returned to normal level after 13th day of administration. 6. The activity of GGT in serum was not changed while the activities of SDH and AST were significantly increased in every test goat and those values were returned to normal after 10~13th day of trestment. 7 The 35K-protein fraction in serum was not detected by SDS-polyacrylamide gel electrophoresis, but this protein fraction was detected by the same method after treating the 21st and 22nd fraction which were obtained by column chromatography with Sephadex G-100. 8. The affected liver was congested and swollen on 3rd day, and yellowish brown in color and mottled appearance on 7th day of treatment. Histopathologically, fat droplets were common in the hepatocytes, this change was intensive on 7th day after treatment in group B and C. Hepatic cell necrosis was observed in some livers but this pathological change was disappeared and returned to normal after 13 days of treatment.

개의 상부 십이지장과 공장이 포함되는 장절제술 일례

이경갑,남치주,성재기,최희인,Lee Kyoung-Kap,Nam Tchi-Chou,Sung Jai-Ki,Choi Hee-In 한국임상수의학회 1988 한국임상수의학회지 Vol.5 No.2

A partial obstruction of small intestine was diagnosed by physical and radiographic examination in a dog which had signs of anorexia, vomiting, diarrhea and dehydration The dog was treated by surgical method. String-like foreign body was located from stomach to upper jejunum. Diffuse laceration, inflammation and perforation caused by foreign body were observed in intestine. Intestinal resection was carried out to remove the foreign body from upper duodenum to upper jejunum. The dog was convalesced successfully after operation.

젖소에서의 간질환 진단을 위한 혈액화학치의 조사

이경갑,최희인,Lee Kyoung-Kap,Choi Hee-In 한국임상수의학회 1986 한국임상수의학회지 Vol.3 No.1

The incidences of liver abscess, fatty liver and fascioliasis were 11.9%, 23.2% and 36.0%, respectively in 211 Holstein cattle observed at the slaughter house. Blood chemical values of them were measured. The results were as follows : 1. Liver abscess group was significantly higher than normal group in the levels of SDH, AST, TP, globulin and fibrinogen, but it was significantly lower than normal group in the levels of albumin and A/G ratio (p<0.01). 2. Fatty liver group was significantly higher than normal group in the levels of SDH, AST, OCT, TP and globulin, but it was significantly lower than normal group in the levels of albumin and A/G ratio (p<0.01). 3. Fasciolisais group was significantly higher than normal group in the levels of AST. TP and globulin (p<0.01), but it was significantly lower than normal group in the levels of albumin and A/G ratio (p<0.01).

적록사슴에서 뿔 및 경정맥 혈액의 성분비교

이경갑,부태삼 濟州大學校 農科大學 動物科學硏究所 1997 動物科學論叢 Vol.12 No.1

The blood components of nok - young(young antler) were examined in the Red Deer. Blood samples were collected from the antler and jugular vein, respectively. The hematological and serum chemical values were as follows; 1. RBC count of nok - hyul(young antler blood) was significantly higher than that of jugular vein blood(p < 0.05). However, fibrinogen concentration of nok - hyul was significantly lower than that of jugular vein blood(p < 0.01). WBC count, packed cell volume(PCV) and total protein(TP) were not significant between nok - hyul and jugular vein blood. 2. The concentration of phosphorous and magnesium of nok - hyul were signhcantly higher than those of jugular vein blood(p < 0.05). Albumin and glutamic oxaloacetic transaminase (GOT) of nok - hyul were hgher than those of jugular vein blood. But globulln and calcium concentration of nok - hyul were lower than those of jugular vein blood. Blood urea nitrogen(BUN) of nok - hyul was not significant when compared with that of jugular vein blood.

하절기 축우의 질병관리

이경갑 한국낙농육우협회 1998 낙농·육우 Vol.18 No.7

개와 고양이 발작증의 치료(2)

이경갑 대한수의사회 1988 대한수의사회지 Vol.24 No.6

소의 혈청 비타민 E 농도와 결핍증에 관한 연구

이경갑,박전홍,임윤규,김희석,이창우,최희인,Lee Hyoung-kap,Park Jun-hong,Lim Yoon-kyu,Kim Hee-seok,Lee Chang-woo,Choi Hee-in 한국임상수의학회 1994 한국임상수의학회지 Vol.11 No.2

Serum $\alpha$-tocopherol was measured in cattle to evaluate normal range and to investigate the difference of $\alpha$-tocopherol levels between healthy cattle and diseased cattle. Seventy two heads of 1 year old beef Cattle have 429.9$\pm$77.2 $\mu\textrm{g}$/100ml of Serum $\alpha$-tocopherol. The Serum $\alpha$-tocopherol values of calves with diarrhea(8 heads), pneumonia(6 heads) and piroplasmosis(4 heads) were 87.1$\pm$19.2, 126.3$\pm$45.7 and 106.3$\pm$30.9$\mu\textrm{g}$/100ml, respectively. But that of calves in good health (S heads) was 357.t$\pm$68.4 $\mu\textrm{g}$/100m1. And the values of diseased calves are significantly lower an that of calves in good health(p<0.05). Seasonaly, serum $\alpha$-tocopherol levels of dairy Holstein cows were 529.9$\pm$ 120.3(March), 540.2$\pm$127.2(June), 566.9$\pm$149.5(September) and 550,0$\pm$ 125.4(December)$\mu\textrm{g}$/100m1, respectively. The values on autumn was the highest than that of othor seasons. Serum H-tocopherol level of cows with retained placenta was 262.2$\pm$40.6$\mu\textrm{g}$/100m1. And the level of retained placenta was significantly lower than that of healthy cattle regardless seasonal variation(p<0.05).

번식우에 대한 rBST투여가 포유기 송아지의 비육과 설사 예방에 미치는 영향

이경갑,류경표,이영재,정종태,김희석,김남중,장병선 한국임상수의학회 1999 한국임상수의학회지 Vol.16 No.2

This study was carried out to investigate weight gain and prevention of diarrhea in suckling calves which were injected with recombinant bovine somatopropin (rBST). A total of 101 breeding cows were assigned to the six groups according to the administered dosage and injected time, respectively. Groups T-1, T-3, T-4 and T-6 were injected starting 1 week before calving and groups T-2 and T-5 were injected on the calving day. The six groups were injected five times at two week intervals. Groups T-1 and T-2 were injected with 250mg composite rBST, Group T-3 was injected with 375 mg composite rBST. Groups T-4 and T-5 were injected with 500mg composite rBST, And group T-6 was injected with 500mg rBST-S. The control group was not injected with BST. The groups injected with 500 mg BrST had a lower rate of morbidity from diarrhea than the control group or the groups injected with 250 mg rBST (T-1 and T-2). Weight gain was higher in group T-4 than in the control group or groups T-1 and T-2. In Korean Native Cattle, the total weight gain was greater in group T-4 than in the control group (p<0.05). In crossbred cows, total weight gain was the highest in group T-4, and the total weight gain rate was greater than in group T-4 and the control group (p<0.05). The results of the hematological values showed that injections of rBST did not affect the level of the RBC, TP and BUN in the breeding cows at 9 weeks after postpartum or the neonatal calves. The results of this study indicate that injecting breeding cows with 500 mg rBST before calving would be effective in the preventing of diarrhea and in increasing weight gain of calves from birth to weaning.