한우 체지방 감소 쇠고기 생산을 위한 복강 및 피하지방 항체 개발

최창원,김유현,김성진,송만강,권응기,오영균,홍성구,최성호,백경훈,Choi, Chang-Weon,Kim, Yu-Hyun,Kim, Sang-Jin,Song, Man-Kang,Kwon, Eung-Gi,Oh, Young-Kyoon,Hong, Seong-Koo,Choi, Seong-Ho,Baek, Kyung-Hoon 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.5

This study aimed to develop polyclonal antibodies to regional inedible adipocytes of Korean native cattle (Hanwoo) and investigate cross-reactivity of the antibodies. Patterns in plasma membrane proteins (PMPs) from abdominal and subcutaneous adipocytes of Hanwoo isolated by collagenase digestion were investigated using SDS-PAGE. As antigens, abdominal and subcutaneous adipocyte PMPs of Hanwoo were injected to sheep 3 times at 3 wk intervals for passive immunization, and non-immunized serum and antisera were collected before and after the injections. Titers of the antisera obtained from sheep and their cross-reactivities with heart, kidney, liver, lung, muscle, and spleen of Hanwoo were determined by ELISA. Isolation and culture of abdominal and subcutaneous adipocytes of Hanwoo were performed for analysing LDH concentration. Based on the SDS-PAGE analysis, specific proteins of PMPs in abdominal and subcutaneous adipocytes appeared despite rather similar patterns between both adipocytes. At the level of 1:1,000 dilution, little antibody reactivity appeared in non-immunized serum whereas the antisera had relatively strong reactivity up to the level of 1:128,000 and 1:64,000 dilution. These findings may indicate that strong antibodies against adipocyte PMPs can be developed using an immunological approach. Extremely low reactivities of abdominal and subcutaneous adipocyte antisera were detected with PMPs of the organs. Both antisera strongly reacted with each adipocyte PMPs and showed statistically (p<0.01) higher cross-reactivities compared with non-immunized serum. In conclusion, these results may indicate that the present polyclonal antibodies against regional inedible adipocyte PMPs are well developed and have safety in cross-reactivities with body organs. Further studies on in vivo cross-reactivity and fat reduction of the antibodies against abdominal and subcutaneous adipocytes PMPs of Hanwoo should be required for inedible fat-reduced high quality beef production.

저지방 고품질 돈육 생산을 위한 돼지 복강 및 피하지방 항체 개발

최창원,백경훈,최창본,오영균,홍성구,Choi, Chang-Weon,Baek, Kyung-Hoon,Choi, Chang-Bon,Oh, Young-Kyoon,Hong, Seong-Koo 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.1

The aim of the present study was to develop polyclonal antibodies to regional inedible adipocytes of pigs and investigate the effect of these antibodies on adipocytes in vitro. As antigens, abdominal and subcutaneous adipocyte PMPs from pigs were injected into sheep 3 times per 3 wk intervals for passive immunization, and non-immunized serum, antisera against abodominal (AAb) or subcutaneous adipocyte PMPs (SAb) were collected before and after the injections. Titers of the antisera obtained from sheep and their cross-reactivities with the heart, kidney, liver, lung, muscle, and spleen of pig were determined by ELISA. Isolation and culture of abdominal and subcutaneous adipocytes from pigs were performed to analyze LDH concentration. At a 1:1,000 dilution, little antibody reactivity was observed for non-immunized serum whereas both AAb and SAb had relatively strong reactivity up to a dilution of 1:16,000. These findings may indicate that strong antibodies against adipocyte PMPs can be developed using an immunological approach. Extremely low reactivity of AAb and SAb was detected with the PMPs of the organs. Both antisera most strongly reacted with each adipocyte PMPs and showed statistically (p<0.05) higher cross-reactivities compared with the non-immunized serum. In conclusion, these results may indicate that the present polyclonal antibodies against regional inedible adipocyte PMPs are well developed and are safe against cross-reactivities with the organs of pigs. Further studies on the in vivo nutritional safety and fat reduction of these antibodies in pigs will be required fat-reduced high quality pork production.

역대의가(歷代醫家)들의 허노(虛勞)에 관한 문헌적(文獻的) 고찰(考察) - 간노(肝勞)를 중심(中心)으로 -

최창원,이강녕,이영수,김희철,곽정진,Choi Chang-Won,Lee Gang-Nyoung,Lee Young-Soo,Kim Hee-Chul,Kwack Jeong-Jin 대한한의학방제학회 2002 大韓韓醫學方劑學會誌 Vol.10 No.1

From the 24 kinds of literature on the Consumptive disease, it can be concluded as follows. 1. The consumptive disease is the Imparement of deficiency type due to overstrain. it is a general term for these all symptom such as and Deficiency of primordial Qi and Essence of life and blood. 2. The excessive fire due to Yin-Deficiency and the injury of spleen-stomach is accounted much of the cause of Consumptive disease. 3. The main cause of the Hepatic asthenia are the Anger, Consumption and over-thinking. 4. The symptoms of the Consumptive disease are mainly caused by the functional disorder of Liver taking charge of tendons. storing and regulating blood, Heart being in charge of blood circulation. taking charge of mental activities. Spleen taking charge of muscles, transforting and transforming nutrients. Lung taking charge of skins and hairs, taking charge of respirations, Kidney taking charge of bones, storing essence of life. 5. The main symptoms of Hepatic asthenia are flaccidity of muscles and temeons which causes limited movement caused by muscular atonia and the loss of bightness of eyes. 6. The main treatments of Consumptive disease are the invigorating the Spleen-stomach and the invigorating the Kidney and storing essence of life. 7. The treatments of Hepatic asthenia are the moderating the middle and the nourishing the muscles and tendons.

사물인터넷 환경에서 IoT 데이터 정합성 연구

최창원,Choi, Changwon 한국사물인터넷학회 2022 한국사물인터넷학회 논문지 Vol.8 No.5

사물인터넷의 발달에 따라 IoT 기기에서 처리되는 데이터의 정확성도 중요시되고 있다. 사물인터넷에서 생산되는 데이터는 센서마다 다양한 포맷과 프로토콜을 사용하고 있기에 수집된 센서 데이터에 이상이 발생하면 정규화하고 통합하는 과정에서 데이터 오류로 인해 실패하거나 잘못된 데이터를 구성하게 된다. 사용자의 상황이나 IoT 기기의 이상 증상은 정확하게 판단되지 않기 때문에 사용자 서비스 장애가 발생하거나 실패하는 문제가 발생한다. 본 논문은 IoT 환경에서 발생되는 다양한 형태의 데이터가 IoT 기기의 특성을 반영하여 정상적인 범주 내에서 변화되는지를 수학적 함수로 산출하여 데이터의 정합성을 탐지하는 방법을 제안한다. IoT 데이터의 발생 특성을 파악하기 위해 '기울기 분석'을 활용한 방법과 '선형 회귀 분석'을 활용한 방법을 각각 제시하고 실험하였다. 기울기를 활용하는 방법은 '증가하는 속도'가 다음에 일어나는 현상에 영향을 미치는 IoT 데이터(센서 기기)에 적합하며, 선형 회귀를 활용하는 방법은 선형적으로 데이터가 움직일 때 '선형 회귀 함수로부터의 차이'가 다음에 일어나는 현상에 영향을 미치는 데이터(수도, 전기 계량기)에 적합하였다.

태아 영아의 생존 한계의 변화에 대한 연구

최창원,황종희,심재원,김성신,고선영,이은경,장윤실,박원순,Choi, Chang Won,Hwang, Jong Hee,Shim, Jae Won,Kim, Sung Sin,Ko, Sun Young,Lee, Eun Kyung,Chang, Yun Sil,Park, Won Soon 대한소아청소년과학회 2003 Clinical and Experimental Pediatrics (CEP) Vol.46 No.12

목 적 : 본 연구는 서울에 소재한 삼성서울병원 신생아중환자실에서의 시기에 따른 태아 영아의 생존 한계의 변화를 알아보기 위해서 시행되었다. 방 법 : 1994년 11월부터 2002년 12월까지 삼성서울병원 신생아중환자실에 입원하였던 출생체중 800 g 미만의 초극소미숙아 73명, 재태연령 27주 미만의 초극소미숙아 117명이 연구대상이었다. 연구대상 초극소미숙아들의 의무기록을 생존여부와 만성폐질환, 미숙아 망막증, 뇌실내 출혈와 뇌실주위 백질연화증을 포함하는 주요 합병증들의 발생유무를 중심으로 조사하였다. 태아 영아의 생존 한계는 생존율이 50%에 도달하는 시점으로 정의하였고 총 연구기간을 1998년 이전(제 1기)과 1999년 이후(제 2기)의 두 시기로 나누어 태아 영아의 생존 한계와 주요 합병증의 발생빈도를 비교하였다. 결 과 : 태아 영아의 생존 한계는 제 1기에는 출생체중 800 g대, 재태연령으로는 26주 대에서 제 2기에는 출생체중 600 g대, 재태연령으로는 24주 대로 현저히 낮아졌다. 제 2기에서의 이 한계 출생체중 대와 재태연령 대에서의 주요 합병증들의 발생빈도는 50% 미만이었다. 주요 합병증들의 발생빈도는 출생체중이나 재태연령이 감소함에 따라서 반드시 증가하지는 않았다. 결 론 : 본 연구의 결과, 본원에서는 출생체중이 600 g 이상이거나 재태연령이 24주 이상인 초극소미숙아들에게는 적극적으로 소생술을 시행해야 할 것으로 생각된다. 이 출생체중과 재태연령의 초극소미숙아에서 주요 합병증이 발생할 위험은 그보다 조금 더 큰 출생체중, 더 많은 재태연령의 그것보다 크지 않은 것으로 보인다. Purpose : To determine the change in the limit of viability in fetal infants and the incidence of major complications in neonatal intensive care unit(NICU) at Samsung Medical Center(SMC). Methods : Sevety-three infants weighing less than 800 g at birth and 117 infants born earlier than 27 weeks gestational age, who were admitted to NICU at SMC between November 1994 and December 2002, were enrolled. Their medical records were reviewed with a focus on the survival and major complications, including chronic lung disease, retinopathy of prematurity, intraventricular hemorrhage, and periventricular leukomalacia. The limit of viability in fetal infants was defined as the birth weight or gestational age at which a 50% survival rate is reached and the incidence of major complications were compared by period(period I : Nov. 1994-Dec. 1998, period II : Jan. 1999-Dec. 2002). Results : The limit of viability in fetal infants was markedly lowered from birth weight range of 800-900 g or gestational age range of $26^{+0}-26^{+6}$ weeks in period I to birth weight range of 600-700 g or gestational age range of $24^{+0}-24^{+6}$ weeks in period II. The incidence rates of major complications at the limit of viability were all less than 50% in period II. There was no definite trend toward increased incidence of major complications as birth weight get smaller and gestational age get younger. Conclusion : Our results indicate that at present, fetal infants whose body weight is over 600 g, or whose gestational age is over 24 weeks should be resuscitated vigorously. The risk of major complications at this birth weight or gestational age seems to be not greater than that at a bigger birth weight or an older gestational age.

ATM 트래픽 특성을 고려한 트래픽 쉐이핑 기법

최창원,김태윤,Choi, Chang-Won,Kim, Tai-Yun 한국정보처리학회 1995 정보처리논문지 Vol.2 No.5

ATM 트래픽의 다중화시 셀 지연 변경(cell delay variance)에 의한 트래픽의 특성 변화로 인하여 셀밉집 현상과 셀 분산 현상이 발생된다. 이러한 현상들은 셀 스트림 의 버스티니스를 증가시키고 네트워크의 폭주 상태를 유발하게 된다. 따라서 입력 스 트림을 일정한 간격으로 네트워크나 트래픽 제어 기능으로 전송하는 트래픽 쉐이핑 기능이 필요하다. 기존 방법들의 대부분이 입력 트래픽을 동일한 특성을 갖는 트래픽 으로 간주하여 연구를 수행하였지만, 본 연구에서는 입력 트래픽을 손실과 지연의 관 점으로 트래픽 특성을 고려한 트래픽 쉐이핑 기법을 제안한다. 입력 트래픽의 특성을 고려한 쉐이핑 모델과 알고리즘을 제시하고 셀 지연 변경의 크기에 따른 트래픽 영향 을 연구한다. 제안된 기법을 가상 스케쥴링 기법(virtual scheduling algorithm)과 비교하여 쉐이핑 기법의 효율성을 평가한다. 실험 결과 제안된 기법이 지연에 민감한 트래픽인 경우 약 12%의 평균 지연의 감소와 손실에 민감한 트래픽인 경우 11%의 버 스티니스의 감소를 보였다. In ATM traffic multiplexing, the cell clumping and the cell dispersion are occured due to the cell delay variance(CDV) which changes the traffic characteristics. These cell variances increase the burstiness of t.he cell streams and make the network congested. The function of the traffic shaping is necessary to transmit the input streams into the networks or into the traffic policing schemes with some intevals. Most of the existing studies regard the input traffics as the traffic with the identical characteristics. In this study, the traffic shaping is processed by considering the traffic characteristICS with t.he loss-sensitive traffic and the delay-sensitive traffic. The traffic shaping model and the traffic shaping algorithm which considers the" characteristics of input streams have been presented. The traffic effect On t.he CDV size is also studied. The proposed scheme is compared to Virtual Scheduling Algorithm(VSA) and the efficiency of the proposed scheme is evaluated. According to the simulation results, t.he mean delay is decreased about 12% in delay-sensitive traffic and the traffic burstiness is decreased about 11 % in loss-sensitive traffic.

Comparison of Cytoplasmic Inclusions Induced by Maize Dwarf Mosaic Virus Strain A and B

최창원,Choi, Chang-Won,Gardner, Wayne S. Korean Society of Electron Microscopy 1994 Applied microscopy Vol.24 No.2

Maize dwarf mosaic virus (MDMV) strain A에 감염된 수수 (Sorghum bicolor L. Moench) 품종 HOK와 strain B에 감염된 Pioneer 8680의 엽세포를 미세구조적으로 비교연구한 결과 종단면과 횡단면으로 초박절편된 표본에서 다양한 원통형 봉입체의 형성이 관찰되었다. MDMV strain A와 B는 세포질 내에 존재하는 특별한 봉입체의 유무에 따라 뚜렷이 구별되었다. MDMV-B에 감염된 Pioneer 8680 수수 엽세포는 pinwheels, bundles, scrolls, 그리고 laminated aggregates 형태의 원통형 봉입체가 관찰된 반면, MDMV-A에 감염된 HOK 수수 엽세포에서는 laminated aggregates가 결여되어 있다. 특히 laminated aggregates의 유무와 관련하여 MDMV 두 strain간에 원통형 봉입체의 미세구조적 차이점은 potyvirus군을 아군으로 분류하는 형태학적 지표가 되어왔다. laminated aggregates가 존재하면 아군 III으로, 존재하지 않을 경우 아군 I로 분류될 수 있다. 이런 원통형 봉입체의 이형화는 바이러스 특이적 유도체로서, 아마도 감염의 발전에 따른 형태 발생을 발현하는 것으로 사료된다. Comparative ultrastructural studies of sorghum (Sorghum bicolor L. Moench) cultivar (cv.) HOK and cv Pioneer 8680 leaf cells separately infected with maize dwarf mosaic virus (MDMV) strain A and B, respectively, revealed the formation of numerous cylindrical inclusions in both cross and longitudinal sections. The MDMV-A and -B were distinguished by the presence or absence of specific inclusion bodies in the cytoplasm. Electron microscope revealed the presence of pinwheels, bundles, scrolls, and laminated aggregates in Pioneer 8680 sorghum leaf cells infected with MDMV-B while no laminated aggregates were found in cells of HOK sorghum leaf cells infected with MDMV-A. Differences in the ultrastructure of cylindrical inclusions between two strains of MDMV, especially with respect to laminated aggregates, have been morphologically indexed to classify potyviruses into subdivision. The presence of laminated aggregates may be assigned to subdivision III while the absence of laminated aggregates is assigned to subdivision I. These variations of structures associated with cylindrical inclusions appeared virus-specific inductions and may be represented the morphogenesis of inclusion bodies following development of infections.

말초신경병증으로 추정되는 비증(痺證) 치험 1례

최창원,Choi, Chang-Won 대한한방내과학회 2009 大韓韓方內科學會誌 Vol.30 No.3

Peripheral neuropathy is a disease of multiple Peripheral nerves. Tingling, pins-and needles, numbness, burning and raw sensation are symptoms of peripheral neuropathy. These symptoms are frequently complained by patients who suffered from obstructive syndrome of Ki and blood (痺證). This case was reported to evaluate the effects of oriental medicine therapy on a patient with obstructive syndrome of Ki and blood (痺證). The subject was a female patient who had obstructive syndrome of Ki and blood (痺證). She complained of calf pain, cold sense, walking discomfort, insomnia, dyspepsia, anorexia etc. We administered the medicine with dispeling the cold pathogenic factor (寒邪), adjusting the constructive and defensive energy (調營衛), tonifying the Ki and blood (補氣血) by stage. After the treatment, the symptoms improved. This case suggests that oriental medicine therapy can be applicable to improve in symptoms with Peripheral neuropathy.

사물인터넷 디바이스의 계정 관리 시스템

최창원,정현철,Choi, Changwon,Chung, Hyuncheol 한국사물인터넷학회 2021 한국사물인터넷학회 논문지 Vol.7 No.1

사물인터넷의 발달에 따라 IOT 장비의 보안 중요성도 더욱 가중되고 있다. 특히 사물인터넷의 특성 상 수십, 수백 개에 이르는 IP 카메라, 홈 IOT 장비, 다양한 측정 장비 등의 계정 관리는 시스템 관리자나 사용자들이 필수적으로 수행해야 하는 번거로운 작업이 되었다. 본 논문에서는 사물인터넷 상에 사용될 수 있는 다양한 장비들의 계정 관리를 체계적으로 설계하여 사용자에게 업무 효율을 높일 수 있는 시스템을 제안한다. 계정 관리의 주요 기능을 6 가지 기능으로 나누어 제공하고 기존의 시스템들보다 개선된 기술들을 적용하였다. 제안 시스템은 권위있는 기관의 인증 테스트를 성공적으로 통과하여 현재 실무 현장에 활용되고 있으며 향후 AI 기술을 적용한 스마트 계정 관리 시스템으로 개발 중이다. As the IOT technology has developed, it becomes more big issues about IOT device security. An account management is a nerve-in-the-box routine job for the system administrator and users who manage the several hundreds IOT devices(IP camera, Home IOT, the various measuring equipment). This study is to propose the account management system by the hierarchical design and it is efficient for the user to manipulate the account management. The designed system supports 6 functions for the account management and apply the advanced technologies for the existed system. After the performance of the designed system is validated successfully by the authoritative test, the designed system is applied for the relative fields. And it is on going for the development of the smart account management system by applying the AI technique.

담배모자이크 바이러스 감염성에 대한 금속의 영향

최창원,Choi, C.W 배재대학교 자연과학연구소 1998 自然科學論文集 Vol.10 No.1

2가 금속인 구리와 아연을 다양한 농도로 각각 담배모자이크 바이러스(TMV)에 처리하여 감염성에 어떤 영향을 주는 가를 조사하였다. TMV의 감염성은 아연의 첨가에 의해 활성화된 반면, 구리의 첨가에 의해 감소되었다. 아연을 첨가한 바이러스 접종원을 담배 잎에 처리한 결과 바이러스만을 접종한 잎보다 더 많은 국부반점이 생성되었다. TMV의 감염성을 감소시키는 구리의 효과는 구리의 농도에 관련되는 결과를 나타내었다. 또한 전기영동으로 다양한 농도의 구리와 아연에 처리된 TMV를 분석한 결과 그 특성이 변화됨이 관찰되었다. 아연 200mM 이상의 농도에 노출시 바이러스는 완전히 분해되었고, 40-20 mM 농도에서는 간신히 바이러스의 흔적을 찾을 수 있으나, 2 mM 농도에서는 상당히 안정되었다. 구리의 경우 20 mM 이하의 농도에 처리되었을 때 구조적으로 완전하게 남아있으나, 100 mM 이상의 처리에서는 완전히 분해되었다. The efficacy of various concentration of divalent copper and zinc ions was evaluated separately for the infectivity of tobacco mosaic virus. Infectivity of TMV was more enhanced by addition of zinc, while it was decreased by addition of copper. The number of local lesions were more produced on tobacco leaves inoculated with inoculum sap containing zinc than those inoculated with sap only. The effect of copper inhibited the infectivity of TMV is dependent on copper concentration. TMV particles treated with various concentration of zinc and copper, respectively, analyzed by electrophoresis, and appeared to be altered in electrophoretic behavior. When TMV was exposed to zinc concentration at more than 200mM, the viral particles were completely degraded, and at 40-20 mM they were barely detectable, but at 2 mM they were quite stable. When TMV was exposed at less than concentration of 20 mM of copper were degraded.