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재래산양 적혈구를 이용한 한우 순환 혈액내 rosette 형성 세포 정량
정기수,김년수,김동훈,강명대,송희종,Cheong, Ki-soo,Kim, Neun-su,Kim, Dong-hoon,Kang, Myoung-dai,Song, Hee-jong 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.4
In order to enumerate the T-lymphocytes in bovine peripheral blood lymphocytes (PBL) by E rosette assay, KGRBC were treated with various concentrations of 2-aminoethylisothiouronium bromide(AET) and dextran(Dex), singly or in combination. To further standardize the assay, optimum concentration of AET- and/or Dex-treatment and incubation time for rosette forming cell(RFC) counts were determined. The levels of B-lymphocytes in the PBL were evaluated by erythzocyte-antibody($EA_{Fc}$)- and erythrocyte-antibody-complement (EAC)-rosetting techniques. The results obtained were as follows; The PBL from 20 clinically normal Korean cattle were formed as low percentage of spontaneous E-rosette ($6.7{\pm}2.4%$) in control group, whereas in KGRBC treated with 0.1M AET for 20 minutes and 8% Dex were formed as $37.3{\pm}2.7%$ and $45.1{\pm}2.1%$, respectively. And the synergistic effects were noted no less than $66.5{\pm}5.6%$ when the KGRBC treated with 0.1M AET and 8% Dex subsequently and rate of RFR did not change significantly between 3~24 hours incubation time at $4^{\circ}C$, EA-and EAC-RFR were $23.3{\pm}9.1%$ and $23.1{\pm}7.9%$, respectively. These results suggest that the KGRBC would be a useful agent for the enumeration of T-lymphocytes by E rosette assay and B-lymphocytes by EA- or EAC-rosette assay in cattle-PBL.
Mucormycosis in a group of chickens
정기수,임병무,Cheong, Ki-soo,Rim, Byung-moo The Korean Society of Veterinary Science 1989 大韓獸醫學會誌 Vol.29 No.1
Mucormycosis는 자연 환경속에 상채하고 있는 Mucorales 목의 곰팡이류에 의해 발생하는 질병인데, 강원도 속초시의 한 양계장에서 82수(연령 100일~2년)의 닭에 집한 발병하였다. 임상중상은 식욕감퇴, 하리, 영양실조, 호흡곤란, 마비 등으로 발병 후 수 주간에 폐사하였으며, 부검시 호흡기관을 비롯하여 간장, 심장, 비장, 신장, 소화관 등에서 회황색의 다발성 결절(0.14~4.6cm)과 소출혈반점을 발견하였다. 병리 조직학적으로 병소는 화농성 혹은 건락성 괴사소와 이를 둘려싸는 위산성 백혈구, 대식세포, 임파구, 거대핵세포의 침륜 등 급성 육아종성염증 소견을 나타내었으며, 본균 특이성의 균사(두께 $4{\sim}24{\mu}$)를 괴사병소 내에서 관찰하였다. 본 질병의 발생은 사료 첨가제와 치료제로써 항생제의 남용이나 타질병의 일차 감염 등이 전소인으로 작용하는 것으로 사료된다. The etiologic agents of mucormycosis are several nonpathogenic fungi of the order Mucorales and are ubiquitous. A total of 82 chickens were infected with the disease and developed anorexia, diarrhea, malnutrition, dyspnea, and paralysis in a chicken farm, Sokcho, Kwangwon. At necropsy there were multiple nodular lesions and hemorrhages in livers, spleens, kidneys, gastrointestinal track, and respiratory system. On histopathological examination it was found that the nodular lesions were consisted of granoulomatous inflammation accompanying characteristic hyphae ($4{\sim}24{\mu}$ wide) of the fungi.
정기수 ( Ki Soo Cheong ),김지태 ( Ji Tae Kim ),이민재 ( Min Jae Lee ),정배동 ( Bae Dong Jung ),안동춘 ( Dong Choon Ahn ),김종택 ( Jong Taek Kim ),김현철 ( Hyeon Cheol Kim ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1
Parasites cause great economic loss in livestock in Korea, and can be categorized as either or indirect losses. In order to monitor the parasites from October 2005 to January 2006, 401 samples were randomly collected from cattle farms and examined the prevalence of gastro-intestinal(GI) parasites by using the sucrose flotation method and formalin-ether centrifugation method. The overall infection rate of GI parasites was about 43.4%. Of these parasites, nematodes were identified as Capillaria bovis (5.5%), Strongyloides papillosus (2.5%), Trichuris discolor (1.2%). Cestoda was observed only one species, Moniezia benedeni (2.2%). The rate of Eimeria spp was 39.2%. A total of five species of Eimeria was identified; among the species identified as above E bovis and E zuernii appeared with relatively higher infection rates. The combined infection of parasites was 35.2% in single species, 29.0% in double and 2.0% in triple infections. No cases of clinical symptoms were observed in this survey. The EPG levels of each species of parasites were very low in all infected cases.
도축우(屠畜牛) 및 도축돈(屠畜豚)에서의 Toxoplasma gondii에 대한 항체(抗體) 조사(調査)
정기수 ( Ki Soo Cheong ),안상철 ( Sang Chul An ),김진옥 ( Jin Ok Kim ),김년수 ( Nyen Su Kim ),장국현 ( Kuk Hyun Chang ) 한국가축위생학회 1994 韓國家畜衛生學會誌 Vol.17 No.1
This study was carried out to investigate antibodies against Toxoplasma gondii by Latex agglutination test from 101 slaugutered pigs and 109 cattle. In the species, 5(4.6%) out of 109 catlle and 4(4.0%) out of 101 pigs were seropositive for Toxoplasma gondii.
최성환,정기수,김인식,태현진,박영재,심정하,안동춘,Choi, Seong-Hwan,Cheong, Ki-Soo,Kim, In-Shik,Tae, Hyun-Jin,Park, Young-Jae,Sim, Jeoung-Ha,Ahn, Dong-Choon 대한수의학회 2006 大韓獸醫學會誌 Vol.46 No.1
This study was aimed to obtain the anatomical information on the location of ostia of left and right coronary artery in 3 weeks old and 6 months old hybrid swine. The each intercommissural distance of 6 months group was twice than 3 weeks old group. The largest sinus was right aortic sinus followed by left and posterior sinus. All left coronary artery ostia in left aortic sinus were located near the right aortic sinus as well as lower than the ostia of right one. Most of the right coronary artery ostia were located at the level of supravalvular ridge of right aortic sinus. In addition the right ostia had more variation than left ones. Comparing to the sites of 3 weeks old pigs, the sites of the right ostia in 6 months group were more variable. These data suggest that the locations of coronary ostia were different with the sites of human's, and changes of the location may be occurred during the growth.
강원도에 집단 도래하는 천연기념물 겨울철새의 생태조사 및 질병발생 가능성에 관한 연구
김종택 ( Jong Taek Kim ),김현철 ( Hyeon Cheol Kim ),박인철 ( In Chul Pak ),정기수 ( Ki Soo Cheong ),장환 ( Hwan Jang ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.3
This report studied the individual number, their favorite habitat, and change pattern of family and group number in Cheolwon Basin, starting in the early Oct. 2004 until late March. 2005 to investigate the ecological features of the Red-crowned Crane and the White-naped Crane. The Cranes arrived Cheolwon Basin in mid-October until next mid-March, and passed the winter in mid-November until next late-February. The most visiting number amounted to 550 individuals and, that period was the most frosting mid-January to mid-February. The White-naped Crane visited Cheolwon Basin in autumn, the early winter and spring. Approximately, the wintering-number is 550 individuals, also, the mid-March was the peak-period of arrival and, the number amounted to 2,162 individuals. The cranes chose the farming area around mountains as their wintering habitat and were less likely to choose the farming area around lake as habitat. The Red-crowned Crane and the White-naped Crane showed the different periodical pattern in familial and group numbers. In case of the crane, the familial pattern was stable, but varied in grouping number, and this pattern was similar to the variation of overall individual number. And, also, the most grouping number was shown in the most freezing period of wintering period. The White-naped Crane showed the similar pattern of the Red-crowned Crane that is, stable family number and varying group number, this pattern affected the total number of entire individuals. Grouping number increased in migration period. Parasite infection rate is G japonensis 35.0%, G vipio 38.7%.
정배동 ( Bae Dong Jung ),박인철 ( In Chul Park ),김정남 ( Jung Nam Kim ),김현철 ( Hyeon Cheol Kim ),정기수 ( Ki Soo Cheong ),김종택 ( Jong Taek Kim ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.4
Eurasian eagle owl (Bubo bubo kiautschensis) is a natural monument (No. 324) and is also classified as an endangered wildlife grade II the Ministry of Environment of Korea. Eurasian eagle owl is threatened by the human being. But the efficient rescue and treatment for Eurasian eagle owl are still problematic, due to lack of basic data. In this study, to obtain basic resources for establishing more efficient rescue system, we held a physical examination and measured size physical part. In addition, we analyzed blood sample and feces samples and autopsy. We conduct the physical examination with measuring tapes and scales for 16 live features and 11 dead features. After inhalation anesthesia take the blood samples from wing vein. Blood samples are analyzed in hematologic and chemistric method. Collect the feces through individual floor of cages from 12 live features and conduct the feces analysis. Autopsy was conducted for 11 dead samples. In physical examination, the mean body weight and total length of live features was 2.26±0.35kg, 61±2.38 ㎝. the mean body weight and total length of dead features was 1.57±0.51kg, 59.31±3.51cm. Mean PCV was 46.57±4.97%, while mean TPP was 3.49±0.57g/dL. In feces sample analysis, 9 of 12 owls(75%) were infested by gastrointestinal helminths. The infection rates were: Trematoda (41.7%), Capilaria sp.(66.7%), acanthocephalans (25%). In autopsy, we confirmed 11 features were infested by duplicated infection of more than 2 helminths. Result of this study will be used for establishing reference range for physical features, blood analysis in Eurasian eagle owl. Therefore, to obtain basic resources for establishing more efficient rescue system, we have to make long-term conservation plans for Eurasian eagle owl.
전자스핀공명법(ESR)을 이용한 방사선조사 조제유류의 판별
운재호 ( Jae Ho Woon ),박병룡 ( Byeong Ryong Park ),최병국 ( Byung Kook Choi ),김나영 ( Na Young Kim ),정홍점 ( Hong Jeom Jeong ),정기수 ( Ki Soo Cheong ),김희선 ( Hee Sun Kim ),김창섭 ( Chang Seob Kim ) 한국유가공기술과학회 2010 Journal of Dairy Science and Biotechnology (JMSB) Vol.28 No.2
Electron Spin Resonance (ESR) spectroscopy has been used to detect the presence of radiation-induced free radicals in biological samples since the mid 1950s and to irradiate foods containing cellulose, crystalline sugar, and bone. Therefore, we analyzed the ESR spectrum of irradiated infant formula and its ingredients in this study. Samples were irradiated with 2 different radiation sources of 60Co gamma rays and electron beams (EBs), and the absorbed doses were 0, 1, 3, 5, and 7 kGy. ESR measurements were performed under normal atmospheric conditions using a JEOL JES-FA100 spectrometer equipped with an X-band bridge. Irradiated infant formula showed anunsymmetrical spectrum (g1=2.0050, g2=2.0006); in contrast, non-irradiated samples showed asymmetrical spectrum. The ingredients of irradiated samples showed a multi-component ESR signal in glucose and lactose and a singlet-type spectrum in milk powder (g=2.0050). R2 of the dose-response curve showed a fine linearity of over 0.95 across the entire sample. We also compared the spectra of identical samples irradiated with 60Co gamma rays and EBs, because EBs can be used for food irradiation in foreign countries, although this is not permitted in Korea. However, we could not find any significant differences according to the types of radiation source. Thus, ESR spectroscopy can be used to detect irradiated infant formula and several types of primary ingredients in this formula.