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김희선,설혜원,안희진,오선경,구승엽,김석현,최영민,김정구,문신용,Kim, Hee-Sun,Seol, Hye-Won,Ahn, Hee-Jin,Oh, Sun-Kyung,Ku, Seung-Yup,Kim, Seok-Hyun,Choi, Young-Min,Kim, Jung-Gu,Moon, Shin-Yong 대한생식의학회 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.4
Objective: This study was performed to evaluate the possibility of prolonged culture of human embryonic stem cells (hESC; SNUhES2) on human amniotic fluid cells (hAFC), which had been storaged after karyotyping. Method: The hAFC was prepared for feeder layer in the presence of Chang's medium and STO medium (90% DMEM, 10% FBS) at $37^{circ}C$ in a 5% $CO_2$ in air atmosphere. Prior to use as a feeder layer, hAFC was mitotically inactivated by mitomycin C. The hESCs on hAFC were passaged mechanically every seven days with ES culture medium (80% DMEM/F12, 20% SR, bFGF). Results: The hAFC feeder layer support the growth of undifferentiated state of SNUhES2 for at least 59 passages thus far. SNUhES2 colonies on hAFC feeder appeared slightly angular and flatter shape as compared with circular and thicker colonies observed with STO feeder layer and showed higher level with complete undifferentiation in seven days. Like hESC cultured on STO feeders, SNUhES2 grown on hAFC expressed normal karyotype, positive for alkaline phosphatase activity, high telomerase activity, Oct-4, SSEA-3, SSEA-4, Tra-1-60 and Tra-1-81 and formed embryoid bodies (EBs). Conclusion: The hAFC supports undifferentiated growth of hESC. Therefore, these results may help to provide a clinically practicable method for expansion of hESC for cell therapies.
하리자돈(下痢子豚) 분변(糞便)에서 Rotavirus 분리(分離) 동정(同定)
김희선,최정옥,김두희,유영수,안수환,Kim, Hee-sun,Choi, Chung-ok,Kim, Doo-hee,Lyoo, Young-soo,An, Soo-hwan 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.1
Twenty-eight porcine rotavirus were isolated from piglets with diarrhoea in chonnam province. According to the age, 41 to 60 day old pigs showed the highest isolation frequency among the post weaning pigs. The characteristics of the field isolates were determined by electronmicroscopy(EM), immunofluorescent assay(FA), and electrophoretic migration patterns of the genome profiles. Some of the isolates showed remarkable haemagglutination activity against rabbit and dog erythrocytes, ranged from 4 to 2848, respectively. At least 3 serotypes of porcine rotavirus were recognized by serum neutralization test using serotype specific rotavirus hyperimmune sera.
국내 야생 집쥐(Rattus norvigicus)의 유행성 출혈열바이러스 감염증의 역학조사
김희선,이준학,강문일,고홍범,Kim, Hee-sun,Graham, Robert Ross,Lee, Jun-hak,Kang, Mun-il,Koh, Hong-bum 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.2
Sixty eight wild rats were caught from Seoul, Kyonggi, Kangwon, Cholla, and Kyongsang provinces in Korea. All of them were confirmed in the same species, Rattus norvigicus. All of sera from wild rats were examined by indirect immunofluorescent test against Hantaan-related virus. Detection of anti-body to Hantaan-related virus were 4 out of 68 rats(5.9%), including 2 of 8(25.0%) rats in Kangwon and 2 of 20 rats(10.0%) in Kyongsang province. No difference of the sero-positive rates between sex of rats tested to Hantaan-related virus was recognized. According to age, four rats with antibodies(1:320~640) to Hantaan-related virus belonged one subadult, one young adult, one middle aged adult, and one old adult, respectively.
국내 야생따쥐(Crocidura lasiura) 의 Hantavirus 항체 보유실태 및 원인체 분리
김희선,강문일,Kim, Hee-sun,Kang, Mun-il 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.1
Eleven shrews were caught from three areas of Korea. All of them were confirmed in the same species, Crocidura lasiura. All of sera from wild shrews were examined by indirect immunofluorescent test against Hantaan-related virus. The antibody to Hantaan-related virus was detected by 2 out of 11 shrews. Just 2 of 7 shrews from BG area were sero-positive for Hantaan-related virus antigen and none from other. All of sero-positive for Hantaan-related virus antigen belonged to male with antibody titer of 1:40 to 1:80. Two Hantaan-related viruses were isolated in vivo and in vitro.
국내 야생 집쥐(Rattus norvegicus)의 murine typhus 감염조사
김희선,강문일,고홍범,Kim, Hee-sun,Graham, Robert Ross,Kang, Mun-il,Koh, Hong-bum 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.4
Sixty eight wild rats, Rattus norvegicus, were caught from Seoul city, Kyonggi, Kangwon, Cholla, and Kyongsang-provinces. All sera collected from rats were examined by immunofluorescent test to find antibody against murine typhus. The antibody prevalance to murine typhus was 4.4%(3/68). Sero-positive rate was 12.5%(2/16) in Kyonggi province and 12.5%(1/8) in Cholla province. The sero-positive rate difference between sexes of rats was not recognized. Three Rattus norvegicus having antibody to murine typhus were morphologically classified as subadult with 8.3%(1/12), middle-aged adult with 5.9%(1/17), and old adult with 4.8%(1/21), respectively.
조직내 효소형 분석에 의한 집쥐류의 분류학적 특성 조사
김희선,강문일,이재일,Kim, Hee-sun,Kang, Mun-il,Lee, Jae-il 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.4
Sixty wild rate (fifty eight of Rattus norvegicus and two R rattus) were caught from Seoul, Kyonggi, Kangwon, Honam, and Yongnam areas between August and October 1992. From liver homogenates of the wild rats, isoenzyme patterns were analysed by starch gel electrophoresis. Using 9 enzyme systems, eight electrophoretic types were identified among wild rats with genetic diversity per locus between 0.00 and 0.49 (Mean 0.15). R rattus from Kyonggi (Kanghwa) and Kangwon (Cholwon) were distinct from R norvegicus from nine regions with 0.581 in genetic divergence. Therefore genetic divergence was different not only in interspecies(0.581) but also in intraspecies(0.111~0.375). These data suggested that isoenzyme electrophoresis could be used as a potential application in taxonomic studies of wild rats.
생쥐 8세포배의 Compaction에 미치는 칼슘 Inhihitor의 영향
김희선,배인하,Kim, Hee-Sun,Bae, In-Ha 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.1
In mammalian development, the embryo which is in the process of compaction, involves a progressive flattening of blastomeres against each other with the consequence that the embryo assumes a spherical shape. This stage happens in the first differentiation. The present study was aimed to examine the involvement of other metal ions in compaction by treating with various divalent cations in the absence of $Ca^{++}$. When 8-cell embryos were cultured in $Ca^{++}$-free medium for 24hrs, they developed to 16-cell stage but did not compaction, and degenerated after 48hrs of culture. Embryos were cultured in $Ca^{++}$-free medium for 24hrs and then transferred to the control medium showed the normal compaction afterwards. When 8-cell embryos were cultured in the presence of $Ni^{++}$, known as a $Ca^{++}$ inhibitor, they cleaved to 16-cell stage but did not compact in the absence of $Ca^{++}$. On the other hand, embryos cultured in the media containing both $Ca^{++}$ and $Ni^{++}$ developed normally so that they underwent compaction during culture for 48hrs. However, they failed to hatch during further 24hrs in the same medium, indicationg that $Ni^{++}$ may exert some harmful effects. Embryos grow in the control medium that contained $Ca^{++}$ but not $Ni^{++}$, developed to the hatched blastocysts. The treatment with $Cd^{++}$ $10^{-1}$,$10^{-2}{\mu}M$, $Mn^{++}$ or $Ba^{++}$ 10,100, $1000{\mu}M$ in $Ca^{++}$-free medium, respectively, inhibited compaction and embryonic degeneration began as in $Ca^{++}$-free medium. When 3, 5, 10mM of $Sr^{++}$, known as a substitute for $Ca^{++}$ in cell, was added to $Ca^{++}$-free medium, respectively, compaction was induced unlike the above metal ions. Embryos were cultured in $Sr^{++}$ developed to blastocysts, but failed to hatch after 72hrs and degenrated. On the other hand, when embryos were cultured in 3, 5, 10mM of $Sr^{++}$ but in $Ca^{++}$-free medium for 24hrs respectively and then transferred to the control, they showed the similiar development as that in the control.
국내 야생 집쥐류(Rattus norvegicus와 R rattus)의 spotted fever group rickettsia 감염에 관한 역학조사
김희선,강문일,Kim, Hee-sun,Kang, Mun-il 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.3
Seventy wild rats were caught from Seoul city, Kyonggi, Kangwon, Honam, and yongnam provinces. All of them were in same species, Rattus norvegicus, except two R rattus from Kyonggi province. Seventy sera from wild rats were studied by immunofluorescent antibody assay for evidence of infection by spotted fever group rickettsia. The antidody prevalance was 37.14%(26/70) for spotted fever group rickettsia. The sero-prevalence rates for spotted fever group rickettsia antibody was the hightest in Kyonggi province with 55.56%(10/18), yongnam province with 50.00%(10/20), Kangwon province with 25.00%(2/8), Seoul city with 18.75%(3/16), and Honam province with 12.50%(1/8). The sero-positive rates difference between sexes were higher in female with 46.15%(12/26) than in male with 31.81%(14/44) for spotted fever group rickettsia. Twenty six of 68 Rattus norvegicus with antibody for spotted fever group rickettsia were in subadult with 50.00%(6/12), young adult with 38.89%(7/18), middle-aged adult with 35.29%(6/17), and old adult with 33.33%(7/21). No antibody was detected from R rattus.
김희선,여인권,Kim, Hee-Sun,Yeo, In-Kwon 한국통계학회 2011 응용통계연구 Vol.24 No.6
주가연계증권(ELS)은 주가와 연동되어 수익률이 결정되는 금융상품으로 현재 증권회사뿐만 아니라 은행에서도 많이 판매되고 있는 간접투자상품이다. 이 논문에서는 수익다이어그램을 통해 대표적인 주가연계증권의 수익구조를 알아보며 특히 현재 가장 많이 판매되고 있는 원금비보장 상품인 스텝다운형에 관심을 가진다. KOSPI 200과 홍콩 HSI를 기초자산으로 설정한 스텝다운형 상품에서 투자시점의 시계열 특성에 따른 조기상환여부와 경험적 분포를 이용한 VaR를 통한 위험률에 대해 고찰해 본다. Equity linked securities are indirect investments where the return of investment depends on the performance of the underlying equities. In this paper, we review the profit structure of typical equity linked securities through a profit diagram and investigate which characteristics of time series at the investment affect the early repayment of the stepdown ELS based on KOSPI 200 and HSI. We also compare VaRs using the empirical distribution function for risk management.