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      • 충청북도에서 분리한 느타리버섯 갈변병 병원세균의 병원성

        이혁인,이승돈,박경선,김영기,차재순 충북대학교 농업과학기술연구소 1997 農業科學硏究 Vol.14 No.-

        One hundred sixty seven bacterial isolates were obtained from the bacterial brown blotch-disea-sed oyster mushrooms collected from 37 mushroom cultivation houses throughout Chungcheungbukdo. The 21(11.9 %) of them formed white line when they were cultured with white line reacting organism(WLRO) and 14 (6.5%) were classified WLRO by white line test. All white line forming isolates against WLRO skewed strong pathogenicity and hemolytic activity to rat red blood cells. Six of the white line forming isolates were identified as Pseudomonas tolaasii which is known a pa-thogen of brown blotch disease of button mushroom (Agaricus bisporus) and oyster mushroom (Pleurotus ostreatus) and four of them were Psudomonas fluorescens type G (biovar V) by BiologR bacterial identification system. WLROs consisted of heterogeneous group of bacteria ; Seven of them were identified as P. fluorescens type A (blovar I) and the rest of them were P. fluorescens type G,P. corrugata, P. tolaasii, and unidentified isolate. Some of the WLROs showed weak pathoge-nicity on rapid pitting test. P. tolaasii A- R and 6041R were mutagenized with transposon No. 5 (Tn5) and the mutants which could not form white line against WLRO were selected. All white line negative Tn5 mutants lost the pathogenicity and ability of hemolysis. The results suggest that tolaasin which is a lipodepsipeptide toxin produced by P. tolaasii and known an causal agent of white line forming in the bacterium is the primary disease determinant of P. tolaasii.

      • KCI등재

        Immunocapture RT-PCR을 이용한 박과작물 종자전염 바이러스의 검출

        이혁인,김정희,예미지 한국식물병리학회 2010 식물병연구 Vol.16 No.2

        Immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) was applied to the detection of Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), and Zucchini green mottle mosaic virus (ZGMMV) on Cucurbitaceae crops. These seed-borne tobamoviruses were accurately detected from the infected leaves and seeds by IC-RT-PCR. This method was estimated to be about 100 times more sensitive than ELISA, and also it allowed the direct confirmation of ELISA results by using the captured antigens from a completed ELISA microwell. This convenient and reliable method could be used routinely for large-scale field surveys or seed tests of Cucurbitaceae crops. 박과작물에 발생하는 종자전염 바이러스 3종(CGMMV, KGMMV, ZGMMV)에 대한 검출을 위해 IC-RT-PCR의 적용 가능성을 시험하였다. IC-RT-PCR을 이용하여 감염 종자와 잎으로 부터 바이러스의 특이적인 검출이 가능하였으며, 검출 민감도는 ELISA 보다 100배 이상 높았다. 또한 반응을 마친 ELISA 마이크로플레이트에 남아있는 항원을 IC-RT-PCR의 template로 사용할 경우 ELISA 결과를 곧바로 확인할 수 있었다. 따라서 IC-RT-PCR에 의한 본 검정방법은 박과작물의 대규모 포장검사 및 종자검사에 편리하게 사용될 수 있을 것으로 기대된다.

      • KCI등재
      • KCI등재

        Mucosal Mast Cell Count Is Associated With Intestinal Permeability in Patients With Diarrhea Predominant Irritable Bowel Syndrome

        이혁,박정호,박동일,김홍주,조용균,손정일,전우규,김병익,채승완 대한소화기 기능성질환∙운동학회 2013 Journal of Neurogastroenterology and Motility (JNM Vol.19 No.2

        Background/Aims Although mucosal mast cell tryptase is known to significantly increase intestinal permeability, the relationship between mucosal mast cells and intestinal permeability remains unclear. The objective of this study was to evaluate the correlation among intestinal permeability, tryptase activity and mucosal mast cell count. Methods Rectal biopsies from 16 patients with diarrhea-predominant irritable bowel syndrome (IBS-D) and 7 normal subjects were assessed for tryptase activity and macromolecular permeability using horseradish peroxidase in Ussing chambers. In addition, mucosal mast cell levels were immunohistochemically quantified via image analysis. Results Rectal biopsy of tissues from IBS-D patients showed significantly increased permeability compared with those from normal controls (0.644 ± 0.08 and 0.06 ± 0.00 ng/2 hr/mm2, P < 0.01). Tryptase activity was also substantially higher in rectal biopsy samples from IBS-D patients than those from normal controls (0.86 ± 0.18 and 0.28 ± 0.04 mU/mg protein, P < 0.05). Mucosal mast cell counts were not significantly different between the 2 groups (P > 0.05). However, correlation analysis revealed that only mucosal mast cell count was significantly correlated with intestinal permeability in IBS-D patients (r = 0.558, P < 0.05). Conclusions This study demonstrated a positive correlation between the number of mucosal mast cells and intestinal permeability, suggesting that mucosal mast cells play an important role for increased intestinal permeability in patients with IBS-D.

      • KCI등재

        차량의 둔턱 진행 모델 강제진동해석과 응답특성

        이혁,윤문철,김종도 한국기계가공학회 2020 한국기계가공학회지 Vol.19 No.11

        The forced vibration characteristics for two impulse forces with time lag was discussed in the vehicle dullprogress model. Detailed numerical analyses of the time domain were performed systematically. By the twoexciting impulse forces, the responses of displacement, the velocity, and the acceleration were investigated indetail for the vehicle’s vibration. Notably, the forced vibration responses in the time domain can be used toidentify and monitor several vehicle vibration models. In order to define the responses of displacement, thevelocity, and the acceleration, we applied a numerical technique (i.e., the Runge–Kutta–Gill method[1,2]). These variables were subsequently used to analyze the vehicle’s vibration according to the time lapse andwhile it passed over a bump stock; moreover, the characteristics of the variables were analyzed in detailaccording to their force conditions. Finally, the intrinsic characteristics of the forced vibration were discussedin the context of the automobile model. Overall, our results indicate that the tested method can besuccessfully applied under different damped conditions.

      • KCI등재후보
      • KCI우수등재

        CUDA를 이용한 BRDF 프로그램의 병렬화 사례 연구

        이혁,백선호,홍혜란,김준성 대한전자공학회 2024 전자공학회논문지 Vol.61 No.3

        컴퓨터 하드웨어의 발전으로 병렬 프로그래밍은 많은 관심을 받으며 응용분야를 확대하고 있다. 특히, GPU를 이용한 병렬 프로그래밍은 시스템 구성 비용이나 구현의 난이도 측면에서 진입장벽이 낮아져 대량의 고속 연산이 필요한 분야에서 필수적이 되었다. 하지만 GPU의 하드웨어적 특성을 고려하지 않은 무분별한 병렬화는 엔지니어의 수고를 무의미하게 만들뿐 아니라 하드웨어가 가진 연산능력을 충분히 활용하지도 못한다. 본 논문에서는 BRDF 프로그램을 대상으로 일반적인 엔지니어를 위한 실질적인 병렬화 접근 방식을 보여준다. CUDA를 사용하여 다양한 수준에서 BRDF 프로그램을 병렬화하고, 몇 가지 하드웨어의 지표를 확인하여 구현된 병렬화가 자원을 적절하게 사용하고 있는지를 확인함으로써 프로그램의 특성과 함께 하드웨어의 특성을 반영하는 병렬 프로그래밍을 통해 더 높은 성능을 나타내도록 개선한다. 실제 환경의 데이터를 사용한 실험은 병렬 프로그래밍에 약간의 수고만으로도 효율적인 병렬컴퓨팅이 가능함을 보여준다. 병렬화 접근 방식과 문제의 크기에 따라 속도 향상은 4.93에서 64.10 사이로 나타난다. 본 논문에서 보여주는 병렬화 접근 방식은 병렬 프로그램을 도입하고자 하는 연구자들이나, 이미 도입은 했지만 더 높은 성능을 얻고자 하는 연구자들에게 도움이 될 것이다. With advancements in computer hardware, there is a growing interest in parallel programming and its widening scope of applications. In particular, parallel programming using GPUs has become essential in fields demanding extensive high-speed computations, thanks to lower entry barriers in terms of system configuration cost and implementation complexity. However, careless parallelization that overlooks the specific characteristics of GPU's hardware makes the efforts of engineers meaningless and fails to fully exploit the hardware's potential. In this paper, we present a practical parallelization approach for general engineers with a BRDF estimation algorithm. We use CUDA to parallelize the BRDF algorithm at various levels and scrutinize several hardware metrics to ensure efficient resource utilization. By taking into account both algorithm and hardware characteristics, we can enhance the performance of parallel programming. Experiments conducted with real-world data demonstrate that efficient computing is possible with little effort in parallel programming. The observed speedup ranges from 4.93 to 64.10 depending on the chosen parallelization approach and problem size. The parallelization process presented in this paper will be helpful to researchers who want to adopt parallel programming for the first time as well as those who have already adopted it and aim for higher performance.

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