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도축돈에서 분리된 Streptococcus suis에 대한 연구
윤선종 ( Sun Jong Yoon ),고홍범 ( Hong Bum Koh ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.3
Streptococcus suis has been identified as a major cause of contagious disease in pigs Ithas been isolated worldwide from pigs suffering from meningitis, bronchopneumonia, polyserositis, polyarthritis and septicemia. Two hundred and fifty-five lung samples of slaughter pigs with gross lung lesions were collected from Jan. to Dec. 1995 in southern Chonnam area. Isolation of S suis were tested by biochemical, serological methods and antimicrobial susceptibility. The results were summerrized as follows; 1. S suis was isolated from 30 of 254 pneumonic lungs, 14 isolates were isolated only, 12 were isolated with P. multocida and 4 were isolated with unidentified Gram positive cocci. 2. In biochemical characteristics studies, all isolates were not grown in 6.5% NaCl medium, and most isolates utilized L-leucine-2-naphtylamide and trehalose, didn`t utilize sorbitol, ribose and L-arabinose. 3. In slide agglutination test, 6(20%) isolates were classified as serotype 2,4(13.3%) isolates were as serotype 1/2, 16,2 and 2(6.6%) were as serotype 1, 4, 13. 4. S suis isolates showed marked susceptibility to amoxicillin, oxacillin, cephalothin and cepofera-zone and high resistance to kanamycin, streptomycin, tetracycline and erythromycin.
권용국,윤선종,김기석 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.2
The toxicity of organophosphate is due to the disruption of the nervous system of birds through the inhibition of cholinesterase enzymes. Two dead white-napped cranes (Grus vipio) was found at Cholwon which is the most popular wintering site in Korea, and submitted to National Veterinary Research and Quarantine Service for diagnosis. Gross examinations revealed no evidence of any specific disease condition. Histopatholgically, only one bird incidentally had multifocal necrotizing hepatitis with intranuclear inclusion bodies in the liver which was considered to be Inclusion Body Disease of cranes. The contents of gastrointestinal tracts of the birds contained residues of parathion ranged as 67.4 and 42.1 ppm respectively, using by gas chromatography and mass spectrometry. Based on these evidences, parathion was responsible for the cranes mortality.
김미경,윤선종,김동규,봉영훈,김희진,장정희,정갑수,Kim, MeeKyung,Yun, Seon Jong,Kim, Dong-Gyu,Bong, Young-Hoon,Kim, Heuijin,Jang, Jung-Hee,Chung, Gab-Soo 대한수의학회 2008 大韓獸醫學會誌 Vol.48 No.2
Pesticides are extensively used for the control of crop pests in agriculture and forestry. Organophosphate (OP) and carbamate pesticides are especially effective for the control of a variety of harmful insects. However, these cholinesterase inhibitors are also dangerous to non-target organisms (wildlife and other animals) because of their high acute toxicity. Most poisonings by pesticides occur as a result of misuse or accidental exposure, but intentional killing of unwanted animals also occurs. At the request of a local autonomous entity, we investigated wild bird poisonings by pesticides from 2003 to 2007. The 207 suspicious samples of pesticide poisoning based on the necropsy were analyzed by GC/NPD, GC/FPD, or GC/MSD. We looked for trends in the identification of pesticides in wild birds thought to have died from poisoning. Pesticides were determined in 59% of the total samples analyzed. Phosphamidon and monochrotophos were the most common pesticides identified, which amounted to 77% of the subtotal. Other OP and carbamate pesticides were also found in various concentrations from dead wild birds. The determined rates of pesticides were as high as 86% and 76% in 2003 and 2006, respectively, during an outbreak of avian influenza in Korea.
A Survey of Epidemic Diseases in Horses Imported into South Korea between 2003 and 2008
이상규,한재익,윤선종,강현구 한국임상수의학회 2010 한국임상수의학회지 Vol.27 No.3
South Korea is susceptible to foreign diseases due to its high rate of livestock importation. The purpose of this study was to investigate the infectious conditions of contagious disease of horses imported into South Korea from other countries. The horses were tested for contagious equine metritis (CEM), equine infectious anemia (EIA),equine piroplasmosis (EP), equine viral arteritis (EVA), vesicular stomatitis (VS), dourine, and glanders. The prevalence of these infectious diseases in 6,650 horses imported from 24 countries between 2003 and 2008 was reviewed by the National Veterinary Research and Quarantine Service. Seropositive results were found for EIA, EP, EVA, dourine and glanders: 3/6,189 serum samples tested were EIA-positive, 37/6,005 samples tested by complement fixation (CF)were EP-positive, 28/6,043 samples tested by virus neutralization (VN) were EVA-positive, 4/2,071 serum samples tested by CF were positive for dourine, and 4/1,950 samples tested by CF were positive for glanders. No contagious equine metritis or vesicular stomatitis was detected. In total, 76/6,650 imported horses tested positive for an infectious disease. Notably, 4/6 sera (66.6%), all taken from horses imported from Tanzania, were positive for glanders. This is the first report of glanders infection in horses from Tanzania since 1996.
장영호,이광직,윤선종,이명헌,송재영,장병준,최농훈 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.2
The efficacies of six commercial disinfectants were evaluated by using Salmonella enterica serovar Typhimurium under simulated natural conditions such as sub-zero temperature, short disinfecting time, and surface type (uneven or smooth). We used a suspensionmodel test to determine the disinfecting efficacy under varying contact times (1, 5, 10, and 30 min) and temperatures (25oC, 4oC, 0oC, and −10oC). The bactericidal effect according to surface structure was measured by using a carriermodel test at 25oC and −10oC. The effective concentrations of each disinfectant were fixed to give a disinfecting effect within a short time (< 1 min) at 25oC and −10oC. The suspension model results revealed that bactericidal efficacy significantly dropped at low temperature for most of the disinfectants used; a sodium dichloroisocyanurate product showed the strongest efficacy. In the carrier test, bacterial load on a wooden surface was more difficult to remove than that on a stainless-steel surface. The results show that commercial disinfectant products vary in their disinfecting efficacy, which is affected by several field factors including temperature, contact time, and carrier material. Environmental conditions and surface type for disinfection should be considered prior to selecting an optimal disinfectant in the field.
김미경,조병훈,김동규,윤선종,임채미,박수정,김희진,김연희,김수연,윤소미,권진욱,손성완,정갑수,이주호,강문일,Kim, MeeKyung,cho, Byung-Hoon,Kim, Dong-Gyu,Yun, Seon Jong,Lim, Chae-Mi,Park, Su-Jeong,Kim, Heuijin,Kim, Yeon Hee,Kim, Soo-Yeon,Yun, So Mi,K 대한수의학회 2006 大韓獸醫學會誌 Vol.46 No.4
Residual materials such as veterinary drugs, environmental contaminants, and pesticides are affecting food safety. High resolution techniques and quality controls are needed to analyze these materials from part per million to part per trillion quantities in food. In order to achieve quality results, standardized methods and techniques are required. Our laboratories were prepared to obtain a certificate of accreditation for ISO/IEC 17025 in the analytical criteria of animal drugs, dioxins, pesticides, and heavy metals. ISO together with IEC has built a strategic partnership with the World Trade Organization with the common goal of promoting a free and fair global trading system. ISO collaborates with the United Nations Organization and its specialized agencies and commissions, particularly those involved in the harmonization of regulations and public policies including the World Health Organization and CODEX Alimentarius for food safety measurement, management and traceability. Our goal was to have high quality analysts, proper analytical methods, good laboratory facilities, and safety systems within guidelines of ISO/IEC 17025. All staff members took requirement exams. We applied proficiency tests in the analysis of veterinary drugs (nitrofuran metabolites, sulfonamide and tetracyclines), dioxins, organophosphorus pesticides, and heavy metals (Cd, Pb, As) to the Food Analysis Performance Assessment Scheme (FAPAS) at Central Science Laboratory, Department for Environment Food and Rural Affairs (DEFRA), England. The results were very satisfactory. All documents were prepared, including system management, laboratory management, standard operational procedures for testing, reporting, and more. The criteria encompassed the requirements of ISO/IEC 17025:1999. Finally, the Korea Laboratory Accreditation Scheme (KOLAS) accredited our testing laboratories in accordance with the provisions of Article 23 of the National Standards Act. The accreditation will give us the benefit of becoming a regional reference laboratory in Asia.